5,5-Dialkylluciferins are thermal stable substrates for bioluminescence-based detection systems

Firefly luciferase-based ATP detection assays are frequently used as a sensitive, cost-efficient method for monitoring hygiene in many industrial settings. Solutions of detection reagent, containing a mixture of a substrate and luciferase enzyme that produces photons in the presence of ATP, are rela...

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Veröffentlicht in:	PloS one 2020-12, Vol.15 (12), p.e0243747-e0243747
Hauptverfasser:	Shi, Ce, Killoran, Michael P, Hall, Mary P, Otto, Paul, Wood, Monika G, Strauss, Ethan, Encell, Lance P, Machleidt, Thomas, Wood, Keith V, Kirkland, Thomas A
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - chemistry Alkylation Ambient temperature ATP Biochemical assays Biological research Biology and Life Sciences Biology, Experimental Bioluminescence Chemical properties Deactivation Engineering and Technology Enzymes Firefly Luciferin - chemistry High resistance Hygiene Inactivation Indicators and Reagents Laboratories Luciferases, Firefly - chemistry Luciferin Luminescent Agents - chemistry Luminescent Measurements - methods Materials Monitoring Monitoring methods Optimization Photons Physical Sciences Protein engineering Reagents Research and Analysis Methods Shelf life Substrate Specificity Substrates Temperature Thermal resistance Thermal stability
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