Development of a multiplex microsphere immunoassay for the detection of antibodies against highly pathogenic viruses in human and animal serum samples
Surveillance of highly pathogenic viruses circulating in both human and animal populations is crucial to unveil endemic infections and potential zoonotic reservoirs. Monitoring the burden of disease by serological assay could be used as an early warning system for imminent outbreaks as an increased...
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| Veröffentlicht in: | PLoS neglected tropical diseases 2020-10, Vol.14 (10), p.e0008699-e0008699 |
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| creator | Surtees, Rebecca Stern, Daniel Ahrens, Katharina Kromarek, Nicole Lander, Angelika Kreher, Petra Weiss, Sabrina Hewson, Roger Punch, Emma K Barr, John N Witkowski, Peter T Couacy-Hymann, Emmanuel Marzi, Andrea Dorner, Brigitte G Kurth, Andreas |
| description | Surveillance of highly pathogenic viruses circulating in both human and animal populations is crucial to unveil endemic infections and potential zoonotic reservoirs. Monitoring the burden of disease by serological assay could be used as an early warning system for imminent outbreaks as an increased seroprevalance often precedes larger outbreaks. However, the multitude of highly pathogenic viruses necessitates the need to identify specific antibodies against several targets from both humans as well as from potential reservoir animals such as bats. In order to address this, we have developed a broadly reactive multiplex microsphere immunoassay (MMIA) for the detection of antibodies against several highly pathogenic viruses from both humans and animals. To this aim, nucleoproteins (NP) of Ebola virus (EBOV), Marburg virus (MARV) and nucleocapsid proteins (NP) of Crimean-Congo haemorrhagic fever virus, Rift Valley fever virus and Dobrava-Belgrade hantavirus were employed in a 5-plex assay for IgG detection. After optimisation, specific binding to each respective NP was shown by testing sera from humans and non-human primates with known infection status. The usefulness of our assay for serosurveillance was shown by determining the immune response against the NP antigens in a panel of 129 human serum samples collected in Guinea between 2011 and 2012 in comparison to a panel of 88 sera from the German blood bank. We found good agreement between our MMIA and commercial or in-house reference methods by ELISA or IIFT with statistically significant higher binding to both EBOV NP and MARV NP coupled microspheres in the Guinea panel. Finally, the MMIA was successfully adapted to detect antibodies from bats that had been inoculated with EBOV- and MARV- virus-like particles, highlighting the versatility of this technique and potentially enabling the monitoring of wildlife as well as human populations with this assay. We were thus able to develop and validate a sensitive and broadly reactive high-throughput serological assay which could be used as a screening tool to detect antibodies against several highly pathogenic viruses. |
| doi_str_mv | 10.1371/journal.pntd.0008699 |
| format | Article |
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Monitoring the burden of disease by serological assay could be used as an early warning system for imminent outbreaks as an increased seroprevalance often precedes larger outbreaks. However, the multitude of highly pathogenic viruses necessitates the need to identify specific antibodies against several targets from both humans as well as from potential reservoir animals such as bats. In order to address this, we have developed a broadly reactive multiplex microsphere immunoassay (MMIA) for the detection of antibodies against several highly pathogenic viruses from both humans and animals. To this aim, nucleoproteins (NP) of Ebola virus (EBOV), Marburg virus (MARV) and nucleocapsid proteins (NP) of Crimean-Congo haemorrhagic fever virus, Rift Valley fever virus and Dobrava-Belgrade hantavirus were employed in a 5-plex assay for IgG detection. After optimisation, specific binding to each respective NP was shown by testing sera from humans and non-human primates with known infection status. The usefulness of our assay for serosurveillance was shown by determining the immune response against the NP antigens in a panel of 129 human serum samples collected in Guinea between 2011 and 2012 in comparison to a panel of 88 sera from the German blood bank. We found good agreement between our MMIA and commercial or in-house reference methods by ELISA or IIFT with statistically significant higher binding to both EBOV NP and MARV NP coupled microspheres in the Guinea panel. Finally, the MMIA was successfully adapted to detect antibodies from bats that had been inoculated with EBOV- and MARV- virus-like particles, highlighting the versatility of this technique and potentially enabling the monitoring of wildlife as well as human populations with this assay. We were thus able to develop and validate a sensitive and broadly reactive high-throughput serological assay which could be used as a screening tool to detect antibodies against several highly pathogenic viruses.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0008699</identifier><identifier>PMID: 33095766</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal population ; Animal populations ; Animals ; Antibodies ; Antibodies, Viral - blood ; Antigens ; Binding ; Biology and life sciences ; Cellular biology ; Chiroptera ; Coccidioidomycosis ; Crimean hemorrhagic fever ; Defence mechanisms ; Detection ; Diagnosis ; Disease ; Early warning systems ; Ebola virus ; ELISA ; Enzyme-linked immunosorbent assay ; Epidemics ; Filoviridae ; Funding ; Hantavirus ; Health aspects ; Health surveillance ; High-throughput screening (Biochemical assaying) ; Human populations ; Humans ; Immune response ; Immune system ; Immunity ; Immunoassay ; Immunoassay - methods ; Immunoassays ; Immunoglobulin G ; Infections ; Inoculation ; Laboratories ; Livestock ; Medicine and Health Sciences ; Methods ; Microspheres ; Monitoring ; Multiplexing ; Nosocomial infections ; Nucleocapsid Proteins - immunology ; Nucleocapsids ; Nucleoproteins ; Optimization ; Orthohantavirus ; Outbreaks ; Pathogens ; Populations ; Primates ; Proteins ; Public health ; Research and Analysis Methods ; Rift Valley fever ; Samples ; Serology ; Serum ; Statistical analysis ; Statistical methods ; Stern, Daniel ; Tropical diseases ; Vector-borne diseases ; Viral antibodies ; Viral diseases ; Virology ; Virus diseases ; Virus Diseases - diagnosis ; Virus Diseases - veterinary ; Virus Diseases - virology ; Virus-like particles ; Viruses ; Wildlife ; Zoonoses</subject><ispartof>PLoS neglected tropical diseases, 2020-10, Vol.14 (10), p.e0008699-e0008699</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication: https://creativecommons.org/publicdomain/zero/1.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c624t-376f660621f44a9d34c5a09b1a535835e339e44e64a85eec34ca0773fa6780f63</citedby><cites>FETCH-LOGICAL-c624t-376f660621f44a9d34c5a09b1a535835e339e44e64a85eec34ca0773fa6780f63</cites><orcidid>0000-0002-4835-3695 ; 0000-0003-0186-9587 ; 0000-0003-0265-8407 ; 0000-0001-9057-4283 ; 0000-0002-5100-2546 ; 0000-0002-6167-8945 ; 0000-0002-9035-2039 ; 0000-0002-5477-6619 ; 0000-0002-6621-6379 ; 0000-0002-9660-9878</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641473/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641473/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33095766$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>McElroy, Anita K.</contributor><creatorcontrib>Surtees, Rebecca</creatorcontrib><creatorcontrib>Stern, Daniel</creatorcontrib><creatorcontrib>Ahrens, Katharina</creatorcontrib><creatorcontrib>Kromarek, Nicole</creatorcontrib><creatorcontrib>Lander, Angelika</creatorcontrib><creatorcontrib>Kreher, Petra</creatorcontrib><creatorcontrib>Weiss, Sabrina</creatorcontrib><creatorcontrib>Hewson, Roger</creatorcontrib><creatorcontrib>Punch, Emma K</creatorcontrib><creatorcontrib>Barr, John N</creatorcontrib><creatorcontrib>Witkowski, Peter T</creatorcontrib><creatorcontrib>Couacy-Hymann, Emmanuel</creatorcontrib><creatorcontrib>Marzi, Andrea</creatorcontrib><creatorcontrib>Dorner, Brigitte G</creatorcontrib><creatorcontrib>Kurth, Andreas</creatorcontrib><title>Development of a multiplex microsphere immunoassay for the detection of antibodies against highly pathogenic viruses in human and animal serum samples</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>Surveillance of highly pathogenic viruses circulating in both human and animal populations is crucial to unveil endemic infections and potential zoonotic reservoirs. Monitoring the burden of disease by serological assay could be used as an early warning system for imminent outbreaks as an increased seroprevalance often precedes larger outbreaks. However, the multitude of highly pathogenic viruses necessitates the need to identify specific antibodies against several targets from both humans as well as from potential reservoir animals such as bats. In order to address this, we have developed a broadly reactive multiplex microsphere immunoassay (MMIA) for the detection of antibodies against several highly pathogenic viruses from both humans and animals. To this aim, nucleoproteins (NP) of Ebola virus (EBOV), Marburg virus (MARV) and nucleocapsid proteins (NP) of Crimean-Congo haemorrhagic fever virus, Rift Valley fever virus and Dobrava-Belgrade hantavirus were employed in a 5-plex assay for IgG detection. After optimisation, specific binding to each respective NP was shown by testing sera from humans and non-human primates with known infection status. The usefulness of our assay for serosurveillance was shown by determining the immune response against the NP antigens in a panel of 129 human serum samples collected in Guinea between 2011 and 2012 in comparison to a panel of 88 sera from the German blood bank. We found good agreement between our MMIA and commercial or in-house reference methods by ELISA or IIFT with statistically significant higher binding to both EBOV NP and MARV NP coupled microspheres in the Guinea panel. Finally, the MMIA was successfully adapted to detect antibodies from bats that had been inoculated with EBOV- and MARV- virus-like particles, highlighting the versatility of this technique and potentially enabling the monitoring of wildlife as well as human populations with this assay. We were thus able to develop and validate a sensitive and broadly reactive high-throughput serological assay which could be used as a screening tool to detect antibodies against several highly pathogenic viruses.</description><subject>Animal population</subject><subject>Animal populations</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Viral - blood</subject><subject>Antigens</subject><subject>Binding</subject><subject>Biology and life sciences</subject><subject>Cellular biology</subject><subject>Chiroptera</subject><subject>Coccidioidomycosis</subject><subject>Crimean hemorrhagic fever</subject><subject>Defence mechanisms</subject><subject>Detection</subject><subject>Diagnosis</subject><subject>Disease</subject><subject>Early warning systems</subject><subject>Ebola virus</subject><subject>ELISA</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Epidemics</subject><subject>Filoviridae</subject><subject>Funding</subject><subject>Hantavirus</subject><subject>Health aspects</subject><subject>Health surveillance</subject><subject>High-throughput screening (Biochemical assaying)</subject><subject>Human populations</subject><subject>Humans</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunity</subject><subject>Immunoassay</subject><subject>Immunoassay - methods</subject><subject>Immunoassays</subject><subject>Immunoglobulin G</subject><subject>Infections</subject><subject>Inoculation</subject><subject>Laboratories</subject><subject>Livestock</subject><subject>Medicine and Health Sciences</subject><subject>Methods</subject><subject>Microspheres</subject><subject>Monitoring</subject><subject>Multiplexing</subject><subject>Nosocomial infections</subject><subject>Nucleocapsid Proteins - immunology</subject><subject>Nucleocapsids</subject><subject>Nucleoproteins</subject><subject>Optimization</subject><subject>Orthohantavirus</subject><subject>Outbreaks</subject><subject>Pathogens</subject><subject>Populations</subject><subject>Primates</subject><subject>Proteins</subject><subject>Public health</subject><subject>Research and Analysis Methods</subject><subject>Rift Valley fever</subject><subject>Samples</subject><subject>Serology</subject><subject>Serum</subject><subject>Statistical analysis</subject><subject>Statistical methods</subject><subject>Stern, Daniel</subject><subject>Tropical diseases</subject><subject>Vector-borne diseases</subject><subject>Viral antibodies</subject><subject>Viral diseases</subject><subject>Virology</subject><subject>Virus diseases</subject><subject>Virus Diseases - diagnosis</subject><subject>Virus Diseases - veterinary</subject><subject>Virus Diseases - virology</subject><subject>Virus-like particles</subject><subject>Viruses</subject><subject>Wildlife</subject><subject>Zoonoses</subject><issn>1935-2735</issn><issn>1935-2727</issn><issn>1935-2735</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>DOA</sourceid><recordid>eNptkt-K1DAUxoso7rr6BqIBQbyZMW3StL0RlvXfwoI3eh1O29M2S5rUJB2cF_F5TXe6y4wsoaQkv-87J4cvSV6ndJuyIv14a2dnQG8nE9otpbQUVfUkOU8rlm-yguVPj_7Pkhfe31KaV3mZPk_OGKNVXghxnvz9jDvUdhrRBGI7AmScdVCTxj9kVI2zfhrQIVHjOBsL3sOedNaRMCBpMWATlDV3QhNUbVuFnkAPyvhABtUPek8mCIPt0aiG7JSbfSSUIcM8gomqNn5qBE08unkkHsZY279MnnWgPb5a94vk19cvP6--b25-fLu-urzZNCLjYcMK0QlBRZZ2nEPVMt7kQKs6hZzlJcuRsQo5R8GhzBGbeA-0KFgHoihpJ9hF8vbgO2nr5TpSLzMuaFWVnNJIXB-I1sKtnFzs1e2lBSXvDqzrJbigGo2Sx0pZK9Kuzjgvi7IuMlpBQbOGdrymdfT6tFab6xHbJs7cgT4xPb0xapC93clC8JQXLBp8WA2c_T2jD3JUvkGtwaCdl75znjJe0jKi7_5DH3_dSvUQH6BMZ2PdZjGVl4LnjGWRi9T2ESquFmNGrMFOxfMTwfsjwYCgw-Ctnpew-FOQH8Alad5h9zCMlMol5vddyyXmco15lL05HuSD6D7X7B_KYfvl</recordid><startdate>20201001</startdate><enddate>20201001</enddate><creator>Surtees, Rebecca</creator><creator>Stern, Daniel</creator><creator>Ahrens, Katharina</creator><creator>Kromarek, Nicole</creator><creator>Lander, Angelika</creator><creator>Kreher, Petra</creator><creator>Weiss, Sabrina</creator><creator>Hewson, Roger</creator><creator>Punch, Emma K</creator><creator>Barr, John N</creator><creator>Witkowski, Peter T</creator><creator>Couacy-Hymann, Emmanuel</creator><creator>Marzi, Andrea</creator><creator>Dorner, Brigitte G</creator><creator>Kurth, Andreas</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SS</scope><scope>7T2</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-4835-3695</orcidid><orcidid>https://orcid.org/0000-0003-0186-9587</orcidid><orcidid>https://orcid.org/0000-0003-0265-8407</orcidid><orcidid>https://orcid.org/0000-0001-9057-4283</orcidid><orcidid>https://orcid.org/0000-0002-5100-2546</orcidid><orcidid>https://orcid.org/0000-0002-6167-8945</orcidid><orcidid>https://orcid.org/0000-0002-9035-2039</orcidid><orcidid>https://orcid.org/0000-0002-5477-6619</orcidid><orcidid>https://orcid.org/0000-0002-6621-6379</orcidid><orcidid>https://orcid.org/0000-0002-9660-9878</orcidid></search><sort><creationdate>20201001</creationdate><title>Development of a multiplex microsphere immunoassay for the detection of antibodies against highly pathogenic viruses in human and animal serum samples</title><author>Surtees, Rebecca ; Stern, Daniel ; Ahrens, Katharina ; Kromarek, Nicole ; Lander, Angelika ; Kreher, Petra ; Weiss, Sabrina ; Hewson, Roger ; Punch, Emma K ; Barr, John N ; Witkowski, Peter T ; Couacy-Hymann, Emmanuel ; Marzi, Andrea ; Dorner, Brigitte G ; Kurth, Andreas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c624t-376f660621f44a9d34c5a09b1a535835e339e44e64a85eec34ca0773fa6780f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animal population</topic><topic>Animal populations</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Viral - blood</topic><topic>Antigens</topic><topic>Binding</topic><topic>Biology and life sciences</topic><topic>Cellular biology</topic><topic>Chiroptera</topic><topic>Coccidioidomycosis</topic><topic>Crimean hemorrhagic fever</topic><topic>Defence mechanisms</topic><topic>Detection</topic><topic>Diagnosis</topic><topic>Disease</topic><topic>Early warning systems</topic><topic>Ebola virus</topic><topic>ELISA</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Epidemics</topic><topic>Filoviridae</topic><topic>Funding</topic><topic>Hantavirus</topic><topic>Health aspects</topic><topic>Health surveillance</topic><topic>High-throughput screening (Biochemical assaying)</topic><topic>Human populations</topic><topic>Humans</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Immunity</topic><topic>Immunoassay</topic><topic>Immunoassay - methods</topic><topic>Immunoassays</topic><topic>Immunoglobulin G</topic><topic>Infections</topic><topic>Inoculation</topic><topic>Laboratories</topic><topic>Livestock</topic><topic>Medicine and Health Sciences</topic><topic>Methods</topic><topic>Microspheres</topic><topic>Monitoring</topic><topic>Multiplexing</topic><topic>Nosocomial infections</topic><topic>Nucleocapsid Proteins - immunology</topic><topic>Nucleocapsids</topic><topic>Nucleoproteins</topic><topic>Optimization</topic><topic>Orthohantavirus</topic><topic>Outbreaks</topic><topic>Pathogens</topic><topic>Populations</topic><topic>Primates</topic><topic>Proteins</topic><topic>Public health</topic><topic>Research and Analysis Methods</topic><topic>Rift Valley fever</topic><topic>Samples</topic><topic>Serology</topic><topic>Serum</topic><topic>Statistical analysis</topic><topic>Statistical methods</topic><topic>Stern, Daniel</topic><topic>Tropical diseases</topic><topic>Vector-borne diseases</topic><topic>Viral antibodies</topic><topic>Viral diseases</topic><topic>Virology</topic><topic>Virus diseases</topic><topic>Virus Diseases - diagnosis</topic><topic>Virus Diseases - veterinary</topic><topic>Virus Diseases - virology</topic><topic>Virus-like particles</topic><topic>Viruses</topic><topic>Wildlife</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Surtees, Rebecca</creatorcontrib><creatorcontrib>Stern, Daniel</creatorcontrib><creatorcontrib>Ahrens, Katharina</creatorcontrib><creatorcontrib>Kromarek, Nicole</creatorcontrib><creatorcontrib>Lander, Angelika</creatorcontrib><creatorcontrib>Kreher, Petra</creatorcontrib><creatorcontrib>Weiss, Sabrina</creatorcontrib><creatorcontrib>Hewson, Roger</creatorcontrib><creatorcontrib>Punch, Emma K</creatorcontrib><creatorcontrib>Barr, John N</creatorcontrib><creatorcontrib>Witkowski, Peter T</creatorcontrib><creatorcontrib>Couacy-Hymann, Emmanuel</creatorcontrib><creatorcontrib>Marzi, Andrea</creatorcontrib><creatorcontrib>Dorner, Brigitte G</creatorcontrib><creatorcontrib>Kurth, Andreas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PLoS neglected tropical diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Surtees, Rebecca</au><au>Stern, Daniel</au><au>Ahrens, Katharina</au><au>Kromarek, Nicole</au><au>Lander, Angelika</au><au>Kreher, Petra</au><au>Weiss, Sabrina</au><au>Hewson, Roger</au><au>Punch, Emma K</au><au>Barr, John N</au><au>Witkowski, Peter T</au><au>Couacy-Hymann, Emmanuel</au><au>Marzi, Andrea</au><au>Dorner, Brigitte G</au><au>Kurth, Andreas</au><au>McElroy, Anita K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a multiplex microsphere immunoassay for the detection of antibodies against highly pathogenic viruses in human and animal serum samples</atitle><jtitle>PLoS neglected tropical diseases</jtitle><addtitle>PLoS Negl Trop Dis</addtitle><date>2020-10-01</date><risdate>2020</risdate><volume>14</volume><issue>10</issue><spage>e0008699</spage><epage>e0008699</epage><pages>e0008699-e0008699</pages><issn>1935-2735</issn><issn>1935-2727</issn><eissn>1935-2735</eissn><abstract>Surveillance of highly pathogenic viruses circulating in both human and animal populations is crucial to unveil endemic infections and potential zoonotic reservoirs. Monitoring the burden of disease by serological assay could be used as an early warning system for imminent outbreaks as an increased seroprevalance often precedes larger outbreaks. However, the multitude of highly pathogenic viruses necessitates the need to identify specific antibodies against several targets from both humans as well as from potential reservoir animals such as bats. In order to address this, we have developed a broadly reactive multiplex microsphere immunoassay (MMIA) for the detection of antibodies against several highly pathogenic viruses from both humans and animals. To this aim, nucleoproteins (NP) of Ebola virus (EBOV), Marburg virus (MARV) and nucleocapsid proteins (NP) of Crimean-Congo haemorrhagic fever virus, Rift Valley fever virus and Dobrava-Belgrade hantavirus were employed in a 5-plex assay for IgG detection. After optimisation, specific binding to each respective NP was shown by testing sera from humans and non-human primates with known infection status. The usefulness of our assay for serosurveillance was shown by determining the immune response against the NP antigens in a panel of 129 human serum samples collected in Guinea between 2011 and 2012 in comparison to a panel of 88 sera from the German blood bank. We found good agreement between our MMIA and commercial or in-house reference methods by ELISA or IIFT with statistically significant higher binding to both EBOV NP and MARV NP coupled microspheres in the Guinea panel. Finally, the MMIA was successfully adapted to detect antibodies from bats that had been inoculated with EBOV- and MARV- virus-like particles, highlighting the versatility of this technique and potentially enabling the monitoring of wildlife as well as human populations with this assay. We were thus able to develop and validate a sensitive and broadly reactive high-throughput serological assay which could be used as a screening tool to detect antibodies against several highly pathogenic viruses.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33095766</pmid><doi>10.1371/journal.pntd.0008699</doi><orcidid>https://orcid.org/0000-0002-4835-3695</orcidid><orcidid>https://orcid.org/0000-0003-0186-9587</orcidid><orcidid>https://orcid.org/0000-0003-0265-8407</orcidid><orcidid>https://orcid.org/0000-0001-9057-4283</orcidid><orcidid>https://orcid.org/0000-0002-5100-2546</orcidid><orcidid>https://orcid.org/0000-0002-6167-8945</orcidid><orcidid>https://orcid.org/0000-0002-9035-2039</orcidid><orcidid>https://orcid.org/0000-0002-5477-6619</orcidid><orcidid>https://orcid.org/0000-0002-6621-6379</orcidid><orcidid>https://orcid.org/0000-0002-9660-9878</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1935-2735 |
| ispartof | PLoS neglected tropical diseases, 2020-10, Vol.14 (10), p.e0008699-e0008699 |
| issn | 1935-2735 1935-2727 1935-2735 |
| language | eng |
| recordid | cdi_plos_journals_2460998400 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Public Library of Science (PLoS); PubMed Central |
| subjects | Animal population Animal populations Animals Antibodies Antibodies, Viral - blood Antigens Binding Biology and life sciences Cellular biology Chiroptera Coccidioidomycosis Crimean hemorrhagic fever Defence mechanisms Detection Diagnosis Disease Early warning systems Ebola virus ELISA Enzyme-linked immunosorbent assay Epidemics Filoviridae Funding Hantavirus Health aspects Health surveillance High-throughput screening (Biochemical assaying) Human populations Humans Immune response Immune system Immunity Immunoassay Immunoassay - methods Immunoassays Immunoglobulin G Infections Inoculation Laboratories Livestock Medicine and Health Sciences Methods Microspheres Monitoring Multiplexing Nosocomial infections Nucleocapsid Proteins - immunology Nucleocapsids Nucleoproteins Optimization Orthohantavirus Outbreaks Pathogens Populations Primates Proteins Public health Research and Analysis Methods Rift Valley fever Samples Serology Serum Statistical analysis Statistical methods Stern, Daniel Tropical diseases Vector-borne diseases Viral antibodies Viral diseases Virology Virus diseases Virus Diseases - diagnosis Virus Diseases - veterinary Virus Diseases - virology Virus-like particles Viruses Wildlife Zoonoses |
| title | Development of a multiplex microsphere immunoassay for the detection of antibodies against highly pathogenic viruses in human and animal serum samples |
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