The route of infection with Leptospira interrogans serovar Copenhageni affects the kinetics of bacterial dissemination and kidney colonization
The goal of this study was to characterize how natural routes of infection affect the kinetics of pathogenic Leptospira dissemination to blood and kidney. C3H/HeJ mice were sublethally infected with L. interrogans serovar Copenhageni FioCruz L1-130 (Leptospira) through exposure of a dermis wound and...
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| creator | Nair, Nisha Guedes, Mariana Soares Werts, Catherine Gomes-Solecki, Maria |
| description | The goal of this study was to characterize how natural routes of infection affect the kinetics of pathogenic Leptospira dissemination to blood and kidney. C3H/HeJ mice were sublethally infected with L. interrogans serovar Copenhageni FioCruz L1-130 (Leptospira) through exposure of a dermis wound and through oral and nasal mucosa, in comparison to uninfected mice and to mice infected via standard intraperitoneal inoculation. In striking contrast to oral mucosa inoculation, transdermal and nasal mucosa infections led to weight loss, renal colonization and inflammation, as previously observed for conjunctival and intraperitoneal infections. However, the timing at which Leptospira gained access to blood, as well as Leptospira' colonization of the kidney and shedding in urine, differed from intraperitoneal infection. Furthermore, a comparative analysis of transcription of pro-inflammatory mediators in kidney and total immunoglobulin isotyping in serum from infected mice, showed increased innate immune response markers (KC, MIP-2, TNF-α) and lower Th1 associated IFN-γ in kidney, as well as lower Th1 associated IgG2a in mice infected through the nasal mucosa as compared to intraperitoneal infection. We conclude that the route of infection affects the timing at which Leptospira gains access to blood for dissemination, as well as the dynamics of colonization and inflammation of the kidney. |
| doi_str_mv | 10.1371/journal.pntd.0007950 |
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C3H/HeJ mice were sublethally infected with L. interrogans serovar Copenhageni FioCruz L1-130 (Leptospira) through exposure of a dermis wound and through oral and nasal mucosa, in comparison to uninfected mice and to mice infected via standard intraperitoneal inoculation. In striking contrast to oral mucosa inoculation, transdermal and nasal mucosa infections led to weight loss, renal colonization and inflammation, as previously observed for conjunctival and intraperitoneal infections. However, the timing at which Leptospira gained access to blood, as well as Leptospira' colonization of the kidney and shedding in urine, differed from intraperitoneal infection. Furthermore, a comparative analysis of transcription of pro-inflammatory mediators in kidney and total immunoglobulin isotyping in serum from infected mice, showed increased innate immune response markers (KC, MIP-2, TNF-α) and lower Th1 associated IFN-γ in kidney, as well as lower Th1 associated IgG2a in mice infected through the nasal mucosa as compared to intraperitoneal infection. We conclude that the route of infection affects the timing at which Leptospira gains access to blood for dissemination, as well as the dynamics of colonization and inflammation of the kidney.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0007950</identifier><identifier>PMID: 31905198</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Access ; Animal biology ; Animals ; Asymptomatic ; Bacteriology ; Biochemistry ; Biology and Life Sciences ; Blood ; Colonization ; Comparative analysis ; Defence mechanisms ; Dermis ; Food contamination & poisoning ; Immune response ; Immune system ; Immunity ; Immunity, Innate ; Immunoglobulin G ; Immunoglobulins ; Immunoglobulins - blood ; Immunology ; Infections ; Inflammation ; Innate immunity ; Inoculation ; Kidneys ; Kinetics ; Laboratory animals ; Leptospira ; Leptospira interrogans ; Leptospira interrogans - immunology ; Leptospira interrogans - physiology ; Leptospirosis ; Leptospirosis - immunology ; Leptospirosis - microbiology ; Leptospirosis - transmission ; Life Sciences ; Lymphocytes T ; Medicine and Health Sciences ; Mice, Inbred C3H ; Microbiology and Parasitology ; Mouth Mucosa ; Mucosa ; Nasal Mucosa ; Nephritis ; Nephritis - immunology ; Nephritis - microbiology ; Nephritis - pathology ; Pathogens ; Research and Analysis Methods ; Serum ; Skin ; Skin - injuries ; Skin - microbiology ; Software ; Transcription ; Tropical diseases ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α ; Urine ; Urine - microbiology ; Veterinary medicine and animal Health ; Weight loss ; Zoonoses ; γ-Interferon</subject><ispartof>PLoS neglected tropical diseases, 2020-01, Vol.14 (1), p.e0007950-e0007950</ispartof><rights>2020 Nair et al. 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C3H/HeJ mice were sublethally infected with L. interrogans serovar Copenhageni FioCruz L1-130 (Leptospira) through exposure of a dermis wound and through oral and nasal mucosa, in comparison to uninfected mice and to mice infected via standard intraperitoneal inoculation. In striking contrast to oral mucosa inoculation, transdermal and nasal mucosa infections led to weight loss, renal colonization and inflammation, as previously observed for conjunctival and intraperitoneal infections. However, the timing at which Leptospira gained access to blood, as well as Leptospira' colonization of the kidney and shedding in urine, differed from intraperitoneal infection. Furthermore, a comparative analysis of transcription of pro-inflammatory mediators in kidney and total immunoglobulin isotyping in serum from infected mice, showed increased innate immune response markers (KC, MIP-2, TNF-α) and lower Th1 associated IFN-γ in kidney, as well as lower Th1 associated IgG2a in mice infected through the nasal mucosa as compared to intraperitoneal infection. 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immunology</subject><subject>Leptospira interrogans - physiology</subject><subject>Leptospirosis</subject><subject>Leptospirosis - immunology</subject><subject>Leptospirosis - microbiology</subject><subject>Leptospirosis - transmission</subject><subject>Life Sciences</subject><subject>Lymphocytes T</subject><subject>Medicine and Health Sciences</subject><subject>Mice, Inbred C3H</subject><subject>Microbiology and Parasitology</subject><subject>Mouth Mucosa</subject><subject>Mucosa</subject><subject>Nasal Mucosa</subject><subject>Nephritis</subject><subject>Nephritis - immunology</subject><subject>Nephritis - microbiology</subject><subject>Nephritis - pathology</subject><subject>Pathogens</subject><subject>Research and Analysis Methods</subject><subject>Serum</subject><subject>Skin</subject><subject>Skin - injuries</subject><subject>Skin - microbiology</subject><subject>Software</subject><subject>Transcription</subject><subject>Tropical diseases</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis factor-α</subject><subject>Urine</subject><subject>Urine - 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blood</topic><topic>Immunology</topic><topic>Infections</topic><topic>Inflammation</topic><topic>Innate immunity</topic><topic>Inoculation</topic><topic>Kidneys</topic><topic>Kinetics</topic><topic>Laboratory animals</topic><topic>Leptospira</topic><topic>Leptospira interrogans</topic><topic>Leptospira interrogans - immunology</topic><topic>Leptospira interrogans - physiology</topic><topic>Leptospirosis</topic><topic>Leptospirosis - immunology</topic><topic>Leptospirosis - microbiology</topic><topic>Leptospirosis - transmission</topic><topic>Life Sciences</topic><topic>Lymphocytes T</topic><topic>Medicine and Health Sciences</topic><topic>Mice, Inbred C3H</topic><topic>Microbiology and Parasitology</topic><topic>Mouth Mucosa</topic><topic>Mucosa</topic><topic>Nasal Mucosa</topic><topic>Nephritis</topic><topic>Nephritis - immunology</topic><topic>Nephritis - microbiology</topic><topic>Nephritis - pathology</topic><topic>Pathogens</topic><topic>Research and Analysis Methods</topic><topic>Serum</topic><topic>Skin</topic><topic>Skin - 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Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PLoS neglected tropical diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nair, Nisha</au><au>Guedes, Mariana Soares</au><au>Werts, Catherine</au><au>Gomes-Solecki, Maria</au><au>Caimano, Melissa J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The route of infection with Leptospira interrogans serovar Copenhageni affects the kinetics of bacterial dissemination and kidney colonization</atitle><jtitle>PLoS neglected tropical diseases</jtitle><addtitle>PLoS Negl Trop Dis</addtitle><date>2020-01-01</date><risdate>2020</risdate><volume>14</volume><issue>1</issue><spage>e0007950</spage><epage>e0007950</epage><pages>e0007950-e0007950</pages><issn>1935-2735</issn><issn>1935-2727</issn><eissn>1935-2735</eissn><abstract>The goal of this study was to characterize how natural routes of infection affect the kinetics of pathogenic Leptospira dissemination to blood and kidney. C3H/HeJ mice were sublethally infected with L. interrogans serovar Copenhageni FioCruz L1-130 (Leptospira) through exposure of a dermis wound and through oral and nasal mucosa, in comparison to uninfected mice and to mice infected via standard intraperitoneal inoculation. In striking contrast to oral mucosa inoculation, transdermal and nasal mucosa infections led to weight loss, renal colonization and inflammation, as previously observed for conjunctival and intraperitoneal infections. However, the timing at which Leptospira gained access to blood, as well as Leptospira' colonization of the kidney and shedding in urine, differed from intraperitoneal infection. Furthermore, a comparative analysis of transcription of pro-inflammatory mediators in kidney and total immunoglobulin isotyping in serum from infected mice, showed increased innate immune response markers (KC, MIP-2, TNF-α) and lower Th1 associated IFN-γ in kidney, as well as lower Th1 associated IgG2a in mice infected through the nasal mucosa as compared to intraperitoneal infection. We conclude that the route of infection affects the timing at which Leptospira gains access to blood for dissemination, as well as the dynamics of colonization and inflammation of the kidney.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31905198</pmid><doi>10.1371/journal.pntd.0007950</doi><orcidid>https://orcid.org/0000-0002-5549-0620</orcidid><orcidid>https://orcid.org/0000-0002-3715-4543</orcidid><orcidid>https://orcid.org/0000-0003-3079-5476</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1935-2735 |
| ispartof | PLoS neglected tropical diseases, 2020-01, Vol.14 (1), p.e0007950-e0007950 |
| issn | 1935-2735 1935-2727 1935-2735 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Public Library of Science (PLoS); PubMed Central |
| subjects | Access Animal biology Animals Asymptomatic Bacteriology Biochemistry Biology and Life Sciences Blood Colonization Comparative analysis Defence mechanisms Dermis Food contamination & poisoning Immune response Immune system Immunity Immunity, Innate Immunoglobulin G Immunoglobulins Immunoglobulins - blood Immunology Infections Inflammation Innate immunity Inoculation Kidneys Kinetics Laboratory animals Leptospira Leptospira interrogans Leptospira interrogans - immunology Leptospira interrogans - physiology Leptospirosis Leptospirosis - immunology Leptospirosis - microbiology Leptospirosis - transmission Life Sciences Lymphocytes T Medicine and Health Sciences Mice, Inbred C3H Microbiology and Parasitology Mouth Mucosa Mucosa Nasal Mucosa Nephritis Nephritis - immunology Nephritis - microbiology Nephritis - pathology Pathogens Research and Analysis Methods Serum Skin Skin - injuries Skin - microbiology Software Transcription Tropical diseases Tumor necrosis factor-TNF Tumor necrosis factor-α Urine Urine - microbiology Veterinary medicine and animal Health Weight loss Zoonoses γ-Interferon |
| title | The route of infection with Leptospira interrogans serovar Copenhageni affects the kinetics of bacterial dissemination and kidney colonization |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T16%3A09%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20route%20of%20infection%20with%20Leptospira%20interrogans%20serovar%20Copenhageni%20affects%20the%20kinetics%20of%20bacterial%20dissemination%20and%20kidney%20colonization&rft.jtitle=PLoS%20neglected%20tropical%20diseases&rft.au=Nair,%20Nisha&rft.date=2020-01-01&rft.volume=14&rft.issue=1&rft.spage=e0007950&rft.epage=e0007950&rft.pages=e0007950-e0007950&rft.issn=1935-2735&rft.eissn=1935-2735&rft_id=info:doi/10.1371/journal.pntd.0007950&rft_dat=%3Cproquest_plos_%3E2334243276%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2460991336&rft_id=info:pmid/31905198&rft_doaj_id=oai_doaj_org_article_e4ca194fc1d040eb8ce7dfb58f399f3d&rfr_iscdi=true |