Improved protocol for efficacious in vitro androgenesis and development of doubled haploids in temperate japonica rice
DH (Doubled haploid) is the immortal mapping population and an outcome of single meiotic cycle, contributed from male partner. An improved procedure was developed for high frequency androgenesis in japonica genotypes, K-332 and GS-88 and their F1s. A total of 207 fertile, green, di-haploid plants we...
Gespeichert in:
| Veröffentlicht in: | PloS one 2020-11, Vol.15 (11), p.e0241292-e0241292 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0241292 |
|---|---|
| container_issue | 11 |
| container_start_page | e0241292 |
| container_title | PloS one |
| container_volume | 15 |
| creator | Sakina, Aafreen Mir, Saba Najeeb, Sofi Zargar, Sajad M Nehvi, Firdous A Rather, Zahoor A Salgotra, Romesh K Shikari, Asif B |
| description | DH (Doubled haploid) is the immortal mapping population and an outcome of single meiotic cycle, contributed from male partner. An improved procedure was developed for high frequency androgenesis in japonica genotypes, K-332 and GS-88 and their F1s. A total of 207 fertile, green, di-haploid plants were generated from K-332 × GS-88 hybrids using the improved anther culture protocol. The investigation was carried out to evaluate callus induction potential and regeneration response for the genotypes and the derived F1s on N6 media and modified N6 media (N6M). Whereas, N6 failed to induce callusing, agarose solidified N6M media supplemented with 4% maltose, growth regulators; NAA (2 mg/l), 2, 4-D (0.5 mg/l), Kinetin (0.5 mg/l), and silver nitrate induced high calli percentage of 27.6% in F1s, 9.5% and 6.7% in GS-88 and K-332 respectively. Murashige and Skoog (MS) media supplemented with 3% sucrose, and the hormonal combination BAP (2 mg/l), Kinetin (1 mg/l) and NAA (1 mg/l) induced high green shoot regeneration rates (0-60.0%). The effect of cold pre-treatment at 4°C and the stage of anther collection and their interaction was studied. The effect of cold pre-treatment (CP) of collected boots at 4°C (for CP2: 2, CP4: 4, CP6: 6 and CP8: 8 days) at different stages of panicle emergence (BES4-6: 4-6, BES7-10: 7-10, BES11-13: 11-13, BES>13: more than 13 inches was worked out in relation to the effect on response of calli induction, albino regeneration, green plant regeneration and number of shoots/green calli. CP referred to the number of days for which the collected boots were incubated before they were inoculated. BES was the length (inches) between flag leaf and penultimate leaf at the time of boot collection. We concluded that CP6 and BES7-10 showed better response to callus proliferation and regeneration of plantlets across genotypes. The appropriate pre-treatment, stage of anther collection and favourable media composition resulted in high calli induction and green plant regeneration rates in recalcitrant japonica genotypes. The modified N6 media resulted into efficient callus induction and is expected to be useful for studies which aim at rapid generation of mapping populations for genetic studies. |
| doi_str_mv | 10.1371/journal.pone.0241292 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2456844757</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A640345247</galeid><doaj_id>oai_doaj_org_article_3052037ec7f34fcd8a66906fcf63c6eb</doaj_id><sourcerecordid>A640345247</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-bf896bf36cc550e354786071d6c95f33ea781c13d831cda7a56cabffbc2cad073</originalsourceid><addsrcrecordid>eNqNk12L3CAUhkNp6W63_QelFQqlvZip0cQkN4Vl6cfAwkK_bsXoccbBxKyaof33NTvZZVP2ouTCqM95PefVk2Uvc7zOaZV_2LvR98KuB9fDGpMiJw15lJ3mDSUrRjB9fO__JHsWwh7jktaMPc1OKE0SdU5Os8OmG7w7gEJpiE46i7TzCLQ2UkjjxoBMjw4meodEr7zbQg_BhGmCFBzAuqGDPiKnkXJja5PSTgzWGXUTGaEbwIsIaC9SpkkUeSPhefZECxvgxTyeZT8_f_px8XV1efVlc3F-uZKsIXHV6rphraZMyrLEQMuiqhmucsVkU2pKQaQqZE5VTXOpRCVKJkWrdSuJFApX9Cx7fdRNGQU-WxY4KUpWF0VVTsTmSCgn9nzwphP-D3fC8JsF57dc-GikBU5xmbysQFaaFlqqWjDWYKalZlQyaJPWx_m0se1AyeSLF3YhutzpzY5v3YFXDKeEWBJ4Nwt4dz1CiLwzQYK1ood0FVPeFakJa4qEvvkHfbi6mdqKVIDptUvnykmUn7MC06IkxUStH6DSp6AzMr0vbdL6IuD9IiAxEX7HrRhD4Jvv3_6fvfq1ZN_eY3cgbNwFZ8doXB-WYHEEpXcheNB3JueYT-1x6waf2oPP7ZHCXt2_oLug236gfwGJVAzm</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2456844757</pqid></control><display><type>article</type><title>Improved protocol for efficacious in vitro androgenesis and development of doubled haploids in temperate japonica rice</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Sakina, Aafreen ; Mir, Saba ; Najeeb, Sofi ; Zargar, Sajad M ; Nehvi, Firdous A ; Rather, Zahoor A ; Salgotra, Romesh K ; Shikari, Asif B</creator><contributor>Kumar, Vijay</contributor><creatorcontrib>Sakina, Aafreen ; Mir, Saba ; Najeeb, Sofi ; Zargar, Sajad M ; Nehvi, Firdous A ; Rather, Zahoor A ; Salgotra, Romesh K ; Shikari, Asif B ; Kumar, Vijay</creatorcontrib><description>DH (Doubled haploid) is the immortal mapping population and an outcome of single meiotic cycle, contributed from male partner. An improved procedure was developed for high frequency androgenesis in japonica genotypes, K-332 and GS-88 and their F1s. A total of 207 fertile, green, di-haploid plants were generated from K-332 × GS-88 hybrids using the improved anther culture protocol. The investigation was carried out to evaluate callus induction potential and regeneration response for the genotypes and the derived F1s on N6 media and modified N6 media (N6M). Whereas, N6 failed to induce callusing, agarose solidified N6M media supplemented with 4% maltose, growth regulators; NAA (2 mg/l), 2, 4-D (0.5 mg/l), Kinetin (0.5 mg/l), and silver nitrate induced high calli percentage of 27.6% in F1s, 9.5% and 6.7% in GS-88 and K-332 respectively. Murashige and Skoog (MS) media supplemented with 3% sucrose, and the hormonal combination BAP (2 mg/l), Kinetin (1 mg/l) and NAA (1 mg/l) induced high green shoot regeneration rates (0-60.0%). The effect of cold pre-treatment at 4°C and the stage of anther collection and their interaction was studied. The effect of cold pre-treatment (CP) of collected boots at 4°C (for CP2: 2, CP4: 4, CP6: 6 and CP8: 8 days) at different stages of panicle emergence (BES4-6: 4-6, BES7-10: 7-10, BES11-13: 11-13, BES>13: more than 13 inches was worked out in relation to the effect on response of calli induction, albino regeneration, green plant regeneration and number of shoots/green calli. CP referred to the number of days for which the collected boots were incubated before they were inoculated. BES was the length (inches) between flag leaf and penultimate leaf at the time of boot collection. We concluded that CP6 and BES7-10 showed better response to callus proliferation and regeneration of plantlets across genotypes. The appropriate pre-treatment, stage of anther collection and favourable media composition resulted in high calli induction and green plant regeneration rates in recalcitrant japonica genotypes. The modified N6 media resulted into efficient callus induction and is expected to be useful for studies which aim at rapid generation of mapping populations for genetic studies.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0241292</identifier><identifier>PMID: 33137812</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>2,4-D ; 2,4-Dichlorophenoxyacetic Acid - metabolism ; Androgenesis ; Androgens - genetics ; Androgens - metabolism ; Biology and Life Sciences ; Biotechnology ; Botanical research ; Callus ; Cold effects ; Cold treatment ; Collection ; Culture Media ; Deoxyribonucleic acid ; DNA ; Engineering and Technology ; Experiments ; Gene mapping ; Genetic aspects ; Genotype & phenotype ; Genotypes ; Growth regulators ; Haploidy ; Hybrids ; In Vitro Techniques ; Kinetin ; Kinetin - genetics ; Leaves ; Maltose ; Mapping ; Media ; Meiosis ; Oryza - genetics ; Oryza - growth & development ; Plant asexual reproduction ; Plant Growth Regulators - metabolism ; Plant Growth Regulators - pharmacology ; Plant Leaves - genetics ; Plant Leaves - growth & development ; Plantlets ; Population genetics ; Population studies ; Properties ; Purines - metabolism ; Regeneration ; Research and Analysis Methods ; Rice ; Shoots ; Silver ; Silver nitrate ; Sucrose ; Sugar</subject><ispartof>PloS one, 2020-11, Vol.15 (11), p.e0241292-e0241292</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Sakina et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Sakina et al 2020 Sakina et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-bf896bf36cc550e354786071d6c95f33ea781c13d831cda7a56cabffbc2cad073</citedby><cites>FETCH-LOGICAL-c692t-bf896bf36cc550e354786071d6c95f33ea781c13d831cda7a56cabffbc2cad073</cites><orcidid>0000-0002-2911-5536</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7605686/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7605686/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33137812$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Kumar, Vijay</contributor><creatorcontrib>Sakina, Aafreen</creatorcontrib><creatorcontrib>Mir, Saba</creatorcontrib><creatorcontrib>Najeeb, Sofi</creatorcontrib><creatorcontrib>Zargar, Sajad M</creatorcontrib><creatorcontrib>Nehvi, Firdous A</creatorcontrib><creatorcontrib>Rather, Zahoor A</creatorcontrib><creatorcontrib>Salgotra, Romesh K</creatorcontrib><creatorcontrib>Shikari, Asif B</creatorcontrib><title>Improved protocol for efficacious in vitro androgenesis and development of doubled haploids in temperate japonica rice</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>DH (Doubled haploid) is the immortal mapping population and an outcome of single meiotic cycle, contributed from male partner. An improved procedure was developed for high frequency androgenesis in japonica genotypes, K-332 and GS-88 and their F1s. A total of 207 fertile, green, di-haploid plants were generated from K-332 × GS-88 hybrids using the improved anther culture protocol. The investigation was carried out to evaluate callus induction potential and regeneration response for the genotypes and the derived F1s on N6 media and modified N6 media (N6M). Whereas, N6 failed to induce callusing, agarose solidified N6M media supplemented with 4% maltose, growth regulators; NAA (2 mg/l), 2, 4-D (0.5 mg/l), Kinetin (0.5 mg/l), and silver nitrate induced high calli percentage of 27.6% in F1s, 9.5% and 6.7% in GS-88 and K-332 respectively. Murashige and Skoog (MS) media supplemented with 3% sucrose, and the hormonal combination BAP (2 mg/l), Kinetin (1 mg/l) and NAA (1 mg/l) induced high green shoot regeneration rates (0-60.0%). The effect of cold pre-treatment at 4°C and the stage of anther collection and their interaction was studied. The effect of cold pre-treatment (CP) of collected boots at 4°C (for CP2: 2, CP4: 4, CP6: 6 and CP8: 8 days) at different stages of panicle emergence (BES4-6: 4-6, BES7-10: 7-10, BES11-13: 11-13, BES>13: more than 13 inches was worked out in relation to the effect on response of calli induction, albino regeneration, green plant regeneration and number of shoots/green calli. CP referred to the number of days for which the collected boots were incubated before they were inoculated. BES was the length (inches) between flag leaf and penultimate leaf at the time of boot collection. We concluded that CP6 and BES7-10 showed better response to callus proliferation and regeneration of plantlets across genotypes. The appropriate pre-treatment, stage of anther collection and favourable media composition resulted in high calli induction and green plant regeneration rates in recalcitrant japonica genotypes. The modified N6 media resulted into efficient callus induction and is expected to be useful for studies which aim at rapid generation of mapping populations for genetic studies.</description><subject>2,4-D</subject><subject>2,4-Dichlorophenoxyacetic Acid - metabolism</subject><subject>Androgenesis</subject><subject>Androgens - genetics</subject><subject>Androgens - metabolism</subject><subject>Biology and Life Sciences</subject><subject>Biotechnology</subject><subject>Botanical research</subject><subject>Callus</subject><subject>Cold effects</subject><subject>Cold treatment</subject><subject>Collection</subject><subject>Culture Media</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Engineering and Technology</subject><subject>Experiments</subject><subject>Gene mapping</subject><subject>Genetic aspects</subject><subject>Genotype & phenotype</subject><subject>Genotypes</subject><subject>Growth regulators</subject><subject>Haploidy</subject><subject>Hybrids</subject><subject>In Vitro Techniques</subject><subject>Kinetin</subject><subject>Kinetin - genetics</subject><subject>Leaves</subject><subject>Maltose</subject><subject>Mapping</subject><subject>Media</subject><subject>Meiosis</subject><subject>Oryza - genetics</subject><subject>Oryza - growth & development</subject><subject>Plant asexual reproduction</subject><subject>Plant Growth Regulators - metabolism</subject><subject>Plant Growth Regulators - pharmacology</subject><subject>Plant Leaves - genetics</subject><subject>Plant Leaves - growth & development</subject><subject>Plantlets</subject><subject>Population genetics</subject><subject>Population studies</subject><subject>Properties</subject><subject>Purines - metabolism</subject><subject>Regeneration</subject><subject>Research and Analysis Methods</subject><subject>Rice</subject><subject>Shoots</subject><subject>Silver</subject><subject>Silver nitrate</subject><subject>Sucrose</subject><subject>Sugar</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L3CAUhkNp6W63_QelFQqlvZip0cQkN4Vl6cfAwkK_bsXoccbBxKyaof33NTvZZVP2ouTCqM95PefVk2Uvc7zOaZV_2LvR98KuB9fDGpMiJw15lJ3mDSUrRjB9fO__JHsWwh7jktaMPc1OKE0SdU5Os8OmG7w7gEJpiE46i7TzCLQ2UkjjxoBMjw4meodEr7zbQg_BhGmCFBzAuqGDPiKnkXJja5PSTgzWGXUTGaEbwIsIaC9SpkkUeSPhefZECxvgxTyeZT8_f_px8XV1efVlc3F-uZKsIXHV6rphraZMyrLEQMuiqhmucsVkU2pKQaQqZE5VTXOpRCVKJkWrdSuJFApX9Cx7fdRNGQU-WxY4KUpWF0VVTsTmSCgn9nzwphP-D3fC8JsF57dc-GikBU5xmbysQFaaFlqqWjDWYKalZlQyaJPWx_m0se1AyeSLF3YhutzpzY5v3YFXDKeEWBJ4Nwt4dz1CiLwzQYK1ood0FVPeFakJa4qEvvkHfbi6mdqKVIDptUvnykmUn7MC06IkxUStH6DSp6AzMr0vbdL6IuD9IiAxEX7HrRhD4Jvv3_6fvfq1ZN_eY3cgbNwFZ8doXB-WYHEEpXcheNB3JueYT-1x6waf2oPP7ZHCXt2_oLug236gfwGJVAzm</recordid><startdate>20201102</startdate><enddate>20201102</enddate><creator>Sakina, Aafreen</creator><creator>Mir, Saba</creator><creator>Najeeb, Sofi</creator><creator>Zargar, Sajad M</creator><creator>Nehvi, Firdous A</creator><creator>Rather, Zahoor A</creator><creator>Salgotra, Romesh K</creator><creator>Shikari, Asif B</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-2911-5536</orcidid></search><sort><creationdate>20201102</creationdate><title>Improved protocol for efficacious in vitro androgenesis and development of doubled haploids in temperate japonica rice</title><author>Sakina, Aafreen ; Mir, Saba ; Najeeb, Sofi ; Zargar, Sajad M ; Nehvi, Firdous A ; Rather, Zahoor A ; Salgotra, Romesh K ; Shikari, Asif B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-bf896bf36cc550e354786071d6c95f33ea781c13d831cda7a56cabffbc2cad073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>2,4-D</topic><topic>2,4-Dichlorophenoxyacetic Acid - metabolism</topic><topic>Androgenesis</topic><topic>Androgens - genetics</topic><topic>Androgens - metabolism</topic><topic>Biology and Life Sciences</topic><topic>Biotechnology</topic><topic>Botanical research</topic><topic>Callus</topic><topic>Cold effects</topic><topic>Cold treatment</topic><topic>Collection</topic><topic>Culture Media</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Engineering and Technology</topic><topic>Experiments</topic><topic>Gene mapping</topic><topic>Genetic aspects</topic><topic>Genotype & phenotype</topic><topic>Genotypes</topic><topic>Growth regulators</topic><topic>Haploidy</topic><topic>Hybrids</topic><topic>In Vitro Techniques</topic><topic>Kinetin</topic><topic>Kinetin - genetics</topic><topic>Leaves</topic><topic>Maltose</topic><topic>Mapping</topic><topic>Media</topic><topic>Meiosis</topic><topic>Oryza - genetics</topic><topic>Oryza - growth & development</topic><topic>Plant asexual reproduction</topic><topic>Plant Growth Regulators - metabolism</topic><topic>Plant Growth Regulators - pharmacology</topic><topic>Plant Leaves - genetics</topic><topic>Plant Leaves - growth & development</topic><topic>Plantlets</topic><topic>Population genetics</topic><topic>Population studies</topic><topic>Properties</topic><topic>Purines - metabolism</topic><topic>Regeneration</topic><topic>Research and Analysis Methods</topic><topic>Rice</topic><topic>Shoots</topic><topic>Silver</topic><topic>Silver nitrate</topic><topic>Sucrose</topic><topic>Sugar</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sakina, Aafreen</creatorcontrib><creatorcontrib>Mir, Saba</creatorcontrib><creatorcontrib>Najeeb, Sofi</creatorcontrib><creatorcontrib>Zargar, Sajad M</creatorcontrib><creatorcontrib>Nehvi, Firdous A</creatorcontrib><creatorcontrib>Rather, Zahoor A</creatorcontrib><creatorcontrib>Salgotra, Romesh K</creatorcontrib><creatorcontrib>Shikari, Asif B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sakina, Aafreen</au><au>Mir, Saba</au><au>Najeeb, Sofi</au><au>Zargar, Sajad M</au><au>Nehvi, Firdous A</au><au>Rather, Zahoor A</au><au>Salgotra, Romesh K</au><au>Shikari, Asif B</au><au>Kumar, Vijay</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved protocol for efficacious in vitro androgenesis and development of doubled haploids in temperate japonica rice</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2020-11-02</date><risdate>2020</risdate><volume>15</volume><issue>11</issue><spage>e0241292</spage><epage>e0241292</epage><pages>e0241292-e0241292</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>DH (Doubled haploid) is the immortal mapping population and an outcome of single meiotic cycle, contributed from male partner. An improved procedure was developed for high frequency androgenesis in japonica genotypes, K-332 and GS-88 and their F1s. A total of 207 fertile, green, di-haploid plants were generated from K-332 × GS-88 hybrids using the improved anther culture protocol. The investigation was carried out to evaluate callus induction potential and regeneration response for the genotypes and the derived F1s on N6 media and modified N6 media (N6M). Whereas, N6 failed to induce callusing, agarose solidified N6M media supplemented with 4% maltose, growth regulators; NAA (2 mg/l), 2, 4-D (0.5 mg/l), Kinetin (0.5 mg/l), and silver nitrate induced high calli percentage of 27.6% in F1s, 9.5% and 6.7% in GS-88 and K-332 respectively. Murashige and Skoog (MS) media supplemented with 3% sucrose, and the hormonal combination BAP (2 mg/l), Kinetin (1 mg/l) and NAA (1 mg/l) induced high green shoot regeneration rates (0-60.0%). The effect of cold pre-treatment at 4°C and the stage of anther collection and their interaction was studied. The effect of cold pre-treatment (CP) of collected boots at 4°C (for CP2: 2, CP4: 4, CP6: 6 and CP8: 8 days) at different stages of panicle emergence (BES4-6: 4-6, BES7-10: 7-10, BES11-13: 11-13, BES>13: more than 13 inches was worked out in relation to the effect on response of calli induction, albino regeneration, green plant regeneration and number of shoots/green calli. CP referred to the number of days for which the collected boots were incubated before they were inoculated. BES was the length (inches) between flag leaf and penultimate leaf at the time of boot collection. We concluded that CP6 and BES7-10 showed better response to callus proliferation and regeneration of plantlets across genotypes. The appropriate pre-treatment, stage of anther collection and favourable media composition resulted in high calli induction and green plant regeneration rates in recalcitrant japonica genotypes. The modified N6 media resulted into efficient callus induction and is expected to be useful for studies which aim at rapid generation of mapping populations for genetic studies.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33137812</pmid><doi>10.1371/journal.pone.0241292</doi><tpages>e0241292</tpages><orcidid>https://orcid.org/0000-0002-2911-5536</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2020-11, Vol.15 (11), p.e0241292-e0241292 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2456844757 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 2,4-D 2,4-Dichlorophenoxyacetic Acid - metabolism Androgenesis Androgens - genetics Androgens - metabolism Biology and Life Sciences Biotechnology Botanical research Callus Cold effects Cold treatment Collection Culture Media Deoxyribonucleic acid DNA Engineering and Technology Experiments Gene mapping Genetic aspects Genotype & phenotype Genotypes Growth regulators Haploidy Hybrids In Vitro Techniques Kinetin Kinetin - genetics Leaves Maltose Mapping Media Meiosis Oryza - genetics Oryza - growth & development Plant asexual reproduction Plant Growth Regulators - metabolism Plant Growth Regulators - pharmacology Plant Leaves - genetics Plant Leaves - growth & development Plantlets Population genetics Population studies Properties Purines - metabolism Regeneration Research and Analysis Methods Rice Shoots Silver Silver nitrate Sucrose Sugar |
| title | Improved protocol for efficacious in vitro androgenesis and development of doubled haploids in temperate japonica rice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T00%3A59%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Improved%20protocol%20for%20efficacious%20in%20vitro%20androgenesis%20and%20development%20of%20doubled%20haploids%20in%20temperate%20japonica%20rice&rft.jtitle=PloS%20one&rft.au=Sakina,%20Aafreen&rft.date=2020-11-02&rft.volume=15&rft.issue=11&rft.spage=e0241292&rft.epage=e0241292&rft.pages=e0241292-e0241292&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0241292&rft_dat=%3Cgale_plos_%3EA640345247%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2456844757&rft_id=info:pmid/33137812&rft_galeid=A640345247&rft_doaj_id=oai_doaj_org_article_3052037ec7f34fcd8a66906fcf63c6eb&rfr_iscdi=true |