Transcriptome analysis of the growth performance of hybrid mandarin fish after food conversion
During recent years, China has become a hotspot for the domestication of mandarin fish, and this is of great commercial value. Although the food preference of domesticated mandarin fish has been studied, little is known about genes regulating their growth. We raised hybrid mandarin fish on artificia...
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| description | During recent years, China has become a hotspot for the domestication of mandarin fish, and this is of great commercial value. Although the food preference of domesticated mandarin fish has been studied, little is known about genes regulating their growth. We raised hybrid mandarin fish on artificial feed for 3 months, the results showed that the survival rate of hybrid mandarin fish was 60.00%. Their total length and body weight were 18.34 ±0.43 cm and 100.44 ±4.87 g. The absolute length and weight gain rates were 0.14 cm/d and 1.08 g/d, respectively. Finally, RNA sequencing (RNA-Seq) was performed to identify potential genes and pathways activated in response to growth performance. The transcriptome analysis generated 68, 197 transcripts and 45,871 unigenes. Among them, 1025 genes were up-regulated and 593 genes were down-regulated between the fast- and slow-growth fish. Finally, we obtained 32 differentially expressed genes, which were mainly related to fatty acid biosynthesis (e.g. FASN and ACACB), collecting duct acid secretion (e.g. ATP6E and KCC4), cell cycle (e.g. CDC20 and CCNB), and the insulin-like growth factor (IGF) system (IGFBP1). These pathways might be related to the growth of hybrid mandarin fish. In addition, more potential single nucleotide polymorphisms (SNPs) were detected in the fast-growth fish than in the slow-growth fish. The results suggest that the interaction of metabolism and abundant alleles might determine the growth of hybrid mandarin fish after food conversion. |
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Although the food preference of domesticated mandarin fish has been studied, little is known about genes regulating their growth. We raised hybrid mandarin fish on artificial feed for 3 months, the results showed that the survival rate of hybrid mandarin fish was 60.00%. Their total length and body weight were 18.34 ±0.43 cm and 100.44 ±4.87 g. The absolute length and weight gain rates were 0.14 cm/d and 1.08 g/d, respectively. Finally, RNA sequencing (RNA-Seq) was performed to identify potential genes and pathways activated in response to growth performance. The transcriptome analysis generated 68, 197 transcripts and 45,871 unigenes. Among them, 1025 genes were up-regulated and 593 genes were down-regulated between the fast- and slow-growth fish. Finally, we obtained 32 differentially expressed genes, which were mainly related to fatty acid biosynthesis (e.g. FASN and ACACB), collecting duct acid secretion (e.g. ATP6E and KCC4), cell cycle (e.g. CDC20 and CCNB), and the insulin-like growth factor (IGF) system (IGFBP1). These pathways might be related to the growth of hybrid mandarin fish. In addition, more potential single nucleotide polymorphisms (SNPs) were detected in the fast-growth fish than in the slow-growth fish. The results suggest that the interaction of metabolism and abundant alleles might determine the growth of hybrid mandarin fish after food conversion.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0240308</identifier><identifier>PMID: 33035258</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Accuracy ; Adaptation ; Analysis ; Animal Feed ; Animals ; Biology and Life Sciences ; Biosynthesis ; Body Weight ; Body weight gain ; Cell cycle ; China ; Collecting duct ; Conversion ; Domestication ; Engineering research ; Fatty acids ; Fish ; Fish Proteins - genetics ; Fisheries ; Fishes - genetics ; Fishes - growth & development ; Food ; Food and nutrition ; Food conversion ; Food preferences ; Gene expression ; Gene Expression Profiling - veterinary ; Gene Expression Regulation, Developmental ; Gene Regulatory Networks ; Gene sequencing ; Genes ; Genetic aspects ; Growth factors ; High-Throughput Nucleotide Sequencing ; Hybrid animals ; Insulin ; Insulin-like growth factor-binding protein 1 ; Insulin-like growth factors ; Laboratory animals ; Mandarinfish ; Metabolism ; Nucleotides ; Physiological aspects ; Research and analysis methods ; Ribonucleic acid ; RNA ; Science education ; Sequence Analysis, RNA ; Single-nucleotide polymorphism ; Survival ; Transcription (Genetics)</subject><ispartof>PloS one, 2020-10, Vol.15 (10), p.e0240308-e0240308</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Guan et al. 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Although the food preference of domesticated mandarin fish has been studied, little is known about genes regulating their growth. We raised hybrid mandarin fish on artificial feed for 3 months, the results showed that the survival rate of hybrid mandarin fish was 60.00%. Their total length and body weight were 18.34 ±0.43 cm and 100.44 ±4.87 g. The absolute length and weight gain rates were 0.14 cm/d and 1.08 g/d, respectively. Finally, RNA sequencing (RNA-Seq) was performed to identify potential genes and pathways activated in response to growth performance. The transcriptome analysis generated 68, 197 transcripts and 45,871 unigenes. Among them, 1025 genes were up-regulated and 593 genes were down-regulated between the fast- and slow-growth fish. 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The results suggest that the interaction of metabolism and abundant alleles might determine the growth of hybrid mandarin fish after food conversion.</description><subject>Accuracy</subject><subject>Adaptation</subject><subject>Analysis</subject><subject>Animal Feed</subject><subject>Animals</subject><subject>Biology and Life Sciences</subject><subject>Biosynthesis</subject><subject>Body Weight</subject><subject>Body weight gain</subject><subject>Cell cycle</subject><subject>China</subject><subject>Collecting duct</subject><subject>Conversion</subject><subject>Domestication</subject><subject>Engineering research</subject><subject>Fatty acids</subject><subject>Fish</subject><subject>Fish Proteins - genetics</subject><subject>Fisheries</subject><subject>Fishes - genetics</subject><subject>Fishes - growth & development</subject><subject>Food</subject><subject>Food and nutrition</subject><subject>Food conversion</subject><subject>Food preferences</subject><subject>Gene expression</subject><subject>Gene Expression Profiling - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guan, Wen-Zhi</au><au>Qiu, Gao-Feng</au><au>Feng-Liu</au><au>Chen, Tzong-Yueh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptome analysis of the growth performance of hybrid mandarin fish after food conversion</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2020-10-09</date><risdate>2020</risdate><volume>15</volume><issue>10</issue><spage>e0240308</spage><epage>e0240308</epage><pages>e0240308-e0240308</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>During recent years, China has become a hotspot for the domestication of mandarin fish, and this is of great commercial value. Although the food preference of domesticated mandarin fish has been studied, little is known about genes regulating their growth. We raised hybrid mandarin fish on artificial feed for 3 months, the results showed that the survival rate of hybrid mandarin fish was 60.00%. Their total length and body weight were 18.34 ±0.43 cm and 100.44 ±4.87 g. The absolute length and weight gain rates were 0.14 cm/d and 1.08 g/d, respectively. Finally, RNA sequencing (RNA-Seq) was performed to identify potential genes and pathways activated in response to growth performance. The transcriptome analysis generated 68, 197 transcripts and 45,871 unigenes. Among them, 1025 genes were up-regulated and 593 genes were down-regulated between the fast- and slow-growth fish. Finally, we obtained 32 differentially expressed genes, which were mainly related to fatty acid biosynthesis (e.g. FASN and ACACB), collecting duct acid secretion (e.g. ATP6E and KCC4), cell cycle (e.g. CDC20 and CCNB), and the insulin-like growth factor (IGF) system (IGFBP1). These pathways might be related to the growth of hybrid mandarin fish. In addition, more potential single nucleotide polymorphisms (SNPs) were detected in the fast-growth fish than in the slow-growth fish. The results suggest that the interaction of metabolism and abundant alleles might determine the growth of hybrid mandarin fish after food conversion.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33035258</pmid><doi>10.1371/journal.pone.0240308</doi><tpages>e0240308</tpages><orcidid>https://orcid.org/0000-0002-6733-8095</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Accuracy Adaptation Analysis Animal Feed Animals Biology and Life Sciences Biosynthesis Body Weight Body weight gain Cell cycle China Collecting duct Conversion Domestication Engineering research Fatty acids Fish Fish Proteins - genetics Fisheries Fishes - genetics Fishes - growth & development Food Food and nutrition Food conversion Food preferences Gene expression Gene Expression Profiling - veterinary Gene Expression Regulation, Developmental Gene Regulatory Networks Gene sequencing Genes Genetic aspects Growth factors High-Throughput Nucleotide Sequencing Hybrid animals Insulin Insulin-like growth factor-binding protein 1 Insulin-like growth factors Laboratory animals Mandarinfish Metabolism Nucleotides Physiological aspects Research and analysis methods Ribonucleic acid RNA Science education Sequence Analysis, RNA Single-nucleotide polymorphism Survival Transcription (Genetics) |
| title | Transcriptome analysis of the growth performance of hybrid mandarin fish after food conversion |
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