Isolation and characterization marine bacteria capable of degrading lignin-derived compounds
Lignin, a characteristic component of terrestrial plants. Rivers transport large amounts of vascular plant organic matter into the oceans where lignin can degrade over time; however, microorganisms involved in this degradation have not been identified. In this study, several bacterial strains were i...
Gespeichert in:
| Veröffentlicht in: | PloS one 2020-10, Vol.15 (10), p.e0240187 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 10 |
| container_start_page | e0240187 |
| container_title | PloS one |
| container_volume | 15 |
| creator | Lu, Peng Wang, Weinan Zhang, Guangxi Li, Wen Jiang, Anjie Cao, Mengjiao Zhang, Xiaoyan Xing, Ke Peng, Xue Yuan, Bo Feng, Zhaozhong |
| description | Lignin, a characteristic component of terrestrial plants. Rivers transport large amounts of vascular plant organic matter into the oceans where lignin can degrade over time; however, microorganisms involved in this degradation have not been identified. In this study, several bacterial strains were isolated from marine samples using the lignin-derived compound vanillic acid (4-hydroxy-3-methoxybenzoic acid) as the sole carbon and energy source. The optimum growth temperature for all isolates ranged from 30 to 35°C. All isolates grew well in a wide NaCl concentration range of 0 to over 50 g/L, with an optimum concentration of 22.8 g/L, which is the same as natural seawater. Phylogenetic analysis indicates that these strains are the members of Halomonas, Arthrobacter, Pseudoalteromonas, Marinomonas, and Thalassospira. These isolates are also able to use other lignin-derived compounds, such as 4-hydroxybenzoic acid, ferulic acid, syringic acid, and benzoic acid. Vanillic acid was detected in all culture media when isolates were grown on ferulic acid as the sole carbon source; however, no 4-hydroxy-3-methoxystyrene was detected, indicating that ferulic acid metabolism by these strains occurs via the elimination of two side chain carbons. Furthermore, the isolates exhibit 3,4-dioxygenase or 4,5-dioxygenase activity for protocatechuic acid ring-cleavage, which is consistent with the genetic sequences of related genera. This study was conducted to isolate and characterize marine bacteria of degrading lignin-derived compounds, thereby revealing the degradation of aromatic compounds in the marine environment and opening up new avenues for the development and utilization of marine biological resources. |
| doi_str_mv | 10.1371/journal.pone.0240187 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2449153789</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A637699242</galeid><doaj_id>oai_doaj_org_article_3d0692cf38cc4a68b85b0aaf0d9ca3f8</doaj_id><sourcerecordid>A637699242</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-3bfc0903612656ced35f0217899de7f975ea83f567f3945d9478a158c294ba023</originalsourceid><addsrcrecordid>eNqNk1uL1TAQx4so7rr6DUQLgujDOebSps2LsCxeDiwseHsSwjSXnhx6kpq0i_rpzbHd5VT2QfKQMPnNfzKTmSx7itEa0wq_2fkxOOjWvXd6jUiBcF3dy04xp2TFCKL3j84n2aMYdwiVtGbsYXZCKSIVxeQ0-76JvoPBepeDU7ncQgA56GB_T8Y9BOt03kxGyCX00HQ69yZXug2grGvzzrbOupVKxLVOIn7f-9Gp-Dh7YKCL-sm8n2Vf37_7cvFxdXn1YXNxfrmSjJNhRRsjEUeUYcJKJrWipUEEVzXnSleGV6WGmpqSVYbyolS8qGrAZS0JLxpAhJ5lzyfdvvNRzIWJghQFxyVNOonYTITysBN9sCmvX8KDFX8NPrQCwmBlpwVVKL1KGlpLWQCrm7psEIBBikugpk5ab-doY7PXSmo3BOgWossbZ7ei9deiKgtUVywJvJoFgv8x6jiIvY1Sdx047cfp3YRhRouEvvgHvTu7mWohJWCd8SmuPIiKc0YrxjkpDlVa30GlpfTeytRFxib7wuH1wiExg_45tDDGKDafP_0_e_Vtyb48YrcaumGbunA8NFxcgsUEyuBjDNrcFhkjcRiCm2qIwxCIeQiS27PjD7p1uul6-gdW7gIE</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2449153789</pqid></control><display><type>article</type><title>Isolation and characterization marine bacteria capable of degrading lignin-derived compounds</title><source>Public Library of Science (PLoS) Journals Open Access</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Lu, Peng ; Wang, Weinan ; Zhang, Guangxi ; Li, Wen ; Jiang, Anjie ; Cao, Mengjiao ; Zhang, Xiaoyan ; Xing, Ke ; Peng, Xue ; Yuan, Bo ; Feng, Zhaozhong</creator><contributor>Arora, Pankaj Kumar</contributor><creatorcontrib>Lu, Peng ; Wang, Weinan ; Zhang, Guangxi ; Li, Wen ; Jiang, Anjie ; Cao, Mengjiao ; Zhang, Xiaoyan ; Xing, Ke ; Peng, Xue ; Yuan, Bo ; Feng, Zhaozhong ; Arora, Pankaj Kumar</creatorcontrib><description>Lignin, a characteristic component of terrestrial plants. Rivers transport large amounts of vascular plant organic matter into the oceans where lignin can degrade over time; however, microorganisms involved in this degradation have not been identified. In this study, several bacterial strains were isolated from marine samples using the lignin-derived compound vanillic acid (4-hydroxy-3-methoxybenzoic acid) as the sole carbon and energy source. The optimum growth temperature for all isolates ranged from 30 to 35°C. All isolates grew well in a wide NaCl concentration range of 0 to over 50 g/L, with an optimum concentration of 22.8 g/L, which is the same as natural seawater. Phylogenetic analysis indicates that these strains are the members of Halomonas, Arthrobacter, Pseudoalteromonas, Marinomonas, and Thalassospira. These isolates are also able to use other lignin-derived compounds, such as 4-hydroxybenzoic acid, ferulic acid, syringic acid, and benzoic acid. Vanillic acid was detected in all culture media when isolates were grown on ferulic acid as the sole carbon source; however, no 4-hydroxy-3-methoxystyrene was detected, indicating that ferulic acid metabolism by these strains occurs via the elimination of two side chain carbons. Furthermore, the isolates exhibit 3,4-dioxygenase or 4,5-dioxygenase activity for protocatechuic acid ring-cleavage, which is consistent with the genetic sequences of related genera. This study was conducted to isolate and characterize marine bacteria of degrading lignin-derived compounds, thereby revealing the degradation of aromatic compounds in the marine environment and opening up new avenues for the development and utilization of marine biological resources.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0240187</identifier><identifier>PMID: 33027312</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Aromatic compounds ; Arthrobacter ; Arthrobacter - metabolism ; Bacteria ; Bacterial Proteins - metabolism ; Benzoic acid ; Benzoic Acid - metabolism ; Biodegradation ; Biology and Life Sciences ; Biomarkers ; Biotransformation ; Carbon ; Carbon sources ; Cellulose ; Chemical analysis ; Chemical properties ; Computer and Information Sciences ; Coumaric Acids - metabolism ; Culture media ; Deoxyribonucleic acid ; Dioxygenase ; Dioxygenases - metabolism ; DNA ; Earth Sciences ; Ecology and Environmental Sciences ; Energy sources ; Environmental degradation ; Enzymes ; Ferulic acid ; Funding ; Gallic Acid - analogs & derivatives ; Gallic Acid - metabolism ; Gene sequencing ; Halomonas - metabolism ; Hydroxybenzoates - metabolism ; Industrial Microbiology - methods ; Life sciences ; Lignin ; Lignin - analogs & derivatives ; Lignin - metabolism ; Marine bacteria ; Marine biology ; Marine environment ; Marine resources ; Marinomonas - metabolism ; Metabolism ; Methods ; Microbiota ; Microorganisms ; Oceans ; Organic matter ; p-Hydroxybenzoic acid ; Phylogenetics ; Phylogeny ; Physical Sciences ; Physiological aspects ; Plants ; Protocatechuic acid ; Pseudoalteromonas - metabolism ; Seawater ; Seawater - microbiology ; Sediments ; Sodium chloride ; Strains (organisms) ; Terrestrial environments ; Vanillic acid ; Water analysis</subject><ispartof>PloS one, 2020-10, Vol.15 (10), p.e0240187</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Lu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Lu et al 2020 Lu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-3bfc0903612656ced35f0217899de7f975ea83f567f3945d9478a158c294ba023</citedby><cites>FETCH-LOGICAL-c692t-3bfc0903612656ced35f0217899de7f975ea83f567f3945d9478a158c294ba023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540876/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540876/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33027312$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Arora, Pankaj Kumar</contributor><creatorcontrib>Lu, Peng</creatorcontrib><creatorcontrib>Wang, Weinan</creatorcontrib><creatorcontrib>Zhang, Guangxi</creatorcontrib><creatorcontrib>Li, Wen</creatorcontrib><creatorcontrib>Jiang, Anjie</creatorcontrib><creatorcontrib>Cao, Mengjiao</creatorcontrib><creatorcontrib>Zhang, Xiaoyan</creatorcontrib><creatorcontrib>Xing, Ke</creatorcontrib><creatorcontrib>Peng, Xue</creatorcontrib><creatorcontrib>Yuan, Bo</creatorcontrib><creatorcontrib>Feng, Zhaozhong</creatorcontrib><title>Isolation and characterization marine bacteria capable of degrading lignin-derived compounds</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Lignin, a characteristic component of terrestrial plants. Rivers transport large amounts of vascular plant organic matter into the oceans where lignin can degrade over time; however, microorganisms involved in this degradation have not been identified. In this study, several bacterial strains were isolated from marine samples using the lignin-derived compound vanillic acid (4-hydroxy-3-methoxybenzoic acid) as the sole carbon and energy source. The optimum growth temperature for all isolates ranged from 30 to 35°C. All isolates grew well in a wide NaCl concentration range of 0 to over 50 g/L, with an optimum concentration of 22.8 g/L, which is the same as natural seawater. Phylogenetic analysis indicates that these strains are the members of Halomonas, Arthrobacter, Pseudoalteromonas, Marinomonas, and Thalassospira. These isolates are also able to use other lignin-derived compounds, such as 4-hydroxybenzoic acid, ferulic acid, syringic acid, and benzoic acid. Vanillic acid was detected in all culture media when isolates were grown on ferulic acid as the sole carbon source; however, no 4-hydroxy-3-methoxystyrene was detected, indicating that ferulic acid metabolism by these strains occurs via the elimination of two side chain carbons. Furthermore, the isolates exhibit 3,4-dioxygenase or 4,5-dioxygenase activity for protocatechuic acid ring-cleavage, which is consistent with the genetic sequences of related genera. This study was conducted to isolate and characterize marine bacteria of degrading lignin-derived compounds, thereby revealing the degradation of aromatic compounds in the marine environment and opening up new avenues for the development and utilization of marine biological resources.</description><subject>Acids</subject><subject>Aromatic compounds</subject><subject>Arthrobacter</subject><subject>Arthrobacter - metabolism</subject><subject>Bacteria</subject><subject>Bacterial Proteins - metabolism</subject><subject>Benzoic acid</subject><subject>Benzoic Acid - metabolism</subject><subject>Biodegradation</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Biotransformation</subject><subject>Carbon</subject><subject>Carbon sources</subject><subject>Cellulose</subject><subject>Chemical analysis</subject><subject>Chemical properties</subject><subject>Computer and Information Sciences</subject><subject>Coumaric Acids - metabolism</subject><subject>Culture media</subject><subject>Deoxyribonucleic acid</subject><subject>Dioxygenase</subject><subject>Dioxygenases - metabolism</subject><subject>DNA</subject><subject>Earth Sciences</subject><subject>Ecology and Environmental Sciences</subject><subject>Energy sources</subject><subject>Environmental degradation</subject><subject>Enzymes</subject><subject>Ferulic acid</subject><subject>Funding</subject><subject>Gallic Acid - analogs & derivatives</subject><subject>Gallic Acid - metabolism</subject><subject>Gene sequencing</subject><subject>Halomonas - metabolism</subject><subject>Hydroxybenzoates - metabolism</subject><subject>Industrial Microbiology - methods</subject><subject>Life sciences</subject><subject>Lignin</subject><subject>Lignin - analogs & derivatives</subject><subject>Lignin - metabolism</subject><subject>Marine bacteria</subject><subject>Marine biology</subject><subject>Marine environment</subject><subject>Marine resources</subject><subject>Marinomonas - metabolism</subject><subject>Metabolism</subject><subject>Methods</subject><subject>Microbiota</subject><subject>Microorganisms</subject><subject>Oceans</subject><subject>Organic matter</subject><subject>p-Hydroxybenzoic acid</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Physical Sciences</subject><subject>Physiological aspects</subject><subject>Plants</subject><subject>Protocatechuic acid</subject><subject>Pseudoalteromonas - metabolism</subject><subject>Seawater</subject><subject>Seawater - microbiology</subject><subject>Sediments</subject><subject>Sodium chloride</subject><subject>Strains (organisms)</subject><subject>Terrestrial environments</subject><subject>Vanillic acid</subject><subject>Water analysis</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1uL1TAQx4so7rr6DUQLgujDOebSps2LsCxeDiwseHsSwjSXnhx6kpq0i_rpzbHd5VT2QfKQMPnNfzKTmSx7itEa0wq_2fkxOOjWvXd6jUiBcF3dy04xp2TFCKL3j84n2aMYdwiVtGbsYXZCKSIVxeQ0-76JvoPBepeDU7ncQgA56GB_T8Y9BOt03kxGyCX00HQ69yZXug2grGvzzrbOupVKxLVOIn7f-9Gp-Dh7YKCL-sm8n2Vf37_7cvFxdXn1YXNxfrmSjJNhRRsjEUeUYcJKJrWipUEEVzXnSleGV6WGmpqSVYbyolS8qGrAZS0JLxpAhJ5lzyfdvvNRzIWJghQFxyVNOonYTITysBN9sCmvX8KDFX8NPrQCwmBlpwVVKL1KGlpLWQCrm7psEIBBikugpk5ab-doY7PXSmo3BOgWossbZ7ei9deiKgtUVywJvJoFgv8x6jiIvY1Sdx047cfp3YRhRouEvvgHvTu7mWohJWCd8SmuPIiKc0YrxjkpDlVa30GlpfTeytRFxib7wuH1wiExg_45tDDGKDafP_0_e_Vtyb48YrcaumGbunA8NFxcgsUEyuBjDNrcFhkjcRiCm2qIwxCIeQiS27PjD7p1uul6-gdW7gIE</recordid><startdate>20201007</startdate><enddate>20201007</enddate><creator>Lu, Peng</creator><creator>Wang, Weinan</creator><creator>Zhang, Guangxi</creator><creator>Li, Wen</creator><creator>Jiang, Anjie</creator><creator>Cao, Mengjiao</creator><creator>Zhang, Xiaoyan</creator><creator>Xing, Ke</creator><creator>Peng, Xue</creator><creator>Yuan, Bo</creator><creator>Feng, Zhaozhong</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20201007</creationdate><title>Isolation and characterization marine bacteria capable of degrading lignin-derived compounds</title><author>Lu, Peng ; Wang, Weinan ; Zhang, Guangxi ; Li, Wen ; Jiang, Anjie ; Cao, Mengjiao ; Zhang, Xiaoyan ; Xing, Ke ; Peng, Xue ; Yuan, Bo ; Feng, Zhaozhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-3bfc0903612656ced35f0217899de7f975ea83f567f3945d9478a158c294ba023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acids</topic><topic>Aromatic compounds</topic><topic>Arthrobacter</topic><topic>Arthrobacter - metabolism</topic><topic>Bacteria</topic><topic>Bacterial Proteins - metabolism</topic><topic>Benzoic acid</topic><topic>Benzoic Acid - metabolism</topic><topic>Biodegradation</topic><topic>Biology and Life Sciences</topic><topic>Biomarkers</topic><topic>Biotransformation</topic><topic>Carbon</topic><topic>Carbon sources</topic><topic>Cellulose</topic><topic>Chemical analysis</topic><topic>Chemical properties</topic><topic>Computer and Information Sciences</topic><topic>Coumaric Acids - metabolism</topic><topic>Culture media</topic><topic>Deoxyribonucleic acid</topic><topic>Dioxygenase</topic><topic>Dioxygenases - metabolism</topic><topic>DNA</topic><topic>Earth Sciences</topic><topic>Ecology and Environmental Sciences</topic><topic>Energy sources</topic><topic>Environmental degradation</topic><topic>Enzymes</topic><topic>Ferulic acid</topic><topic>Funding</topic><topic>Gallic Acid - analogs & derivatives</topic><topic>Gallic Acid - metabolism</topic><topic>Gene sequencing</topic><topic>Halomonas - metabolism</topic><topic>Hydroxybenzoates - metabolism</topic><topic>Industrial Microbiology - methods</topic><topic>Life sciences</topic><topic>Lignin</topic><topic>Lignin - analogs & derivatives</topic><topic>Lignin - metabolism</topic><topic>Marine bacteria</topic><topic>Marine biology</topic><topic>Marine environment</topic><topic>Marine resources</topic><topic>Marinomonas - metabolism</topic><topic>Metabolism</topic><topic>Methods</topic><topic>Microbiota</topic><topic>Microorganisms</topic><topic>Oceans</topic><topic>Organic matter</topic><topic>p-Hydroxybenzoic acid</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Physical Sciences</topic><topic>Physiological aspects</topic><topic>Plants</topic><topic>Protocatechuic acid</topic><topic>Pseudoalteromonas - metabolism</topic><topic>Seawater</topic><topic>Seawater - microbiology</topic><topic>Sediments</topic><topic>Sodium chloride</topic><topic>Strains (organisms)</topic><topic>Terrestrial environments</topic><topic>Vanillic acid</topic><topic>Water analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Peng</creatorcontrib><creatorcontrib>Wang, Weinan</creatorcontrib><creatorcontrib>Zhang, Guangxi</creatorcontrib><creatorcontrib>Li, Wen</creatorcontrib><creatorcontrib>Jiang, Anjie</creatorcontrib><creatorcontrib>Cao, Mengjiao</creatorcontrib><creatorcontrib>Zhang, Xiaoyan</creatorcontrib><creatorcontrib>Xing, Ke</creatorcontrib><creatorcontrib>Peng, Xue</creatorcontrib><creatorcontrib>Yuan, Bo</creatorcontrib><creatorcontrib>Feng, Zhaozhong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Opposing Viewpoints in Context (Gale)</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Peng</au><au>Wang, Weinan</au><au>Zhang, Guangxi</au><au>Li, Wen</au><au>Jiang, Anjie</au><au>Cao, Mengjiao</au><au>Zhang, Xiaoyan</au><au>Xing, Ke</au><au>Peng, Xue</au><au>Yuan, Bo</au><au>Feng, Zhaozhong</au><au>Arora, Pankaj Kumar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization marine bacteria capable of degrading lignin-derived compounds</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2020-10-07</date><risdate>2020</risdate><volume>15</volume><issue>10</issue><spage>e0240187</spage><pages>e0240187-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Lignin, a characteristic component of terrestrial plants. Rivers transport large amounts of vascular plant organic matter into the oceans where lignin can degrade over time; however, microorganisms involved in this degradation have not been identified. In this study, several bacterial strains were isolated from marine samples using the lignin-derived compound vanillic acid (4-hydroxy-3-methoxybenzoic acid) as the sole carbon and energy source. The optimum growth temperature for all isolates ranged from 30 to 35°C. All isolates grew well in a wide NaCl concentration range of 0 to over 50 g/L, with an optimum concentration of 22.8 g/L, which is the same as natural seawater. Phylogenetic analysis indicates that these strains are the members of Halomonas, Arthrobacter, Pseudoalteromonas, Marinomonas, and Thalassospira. These isolates are also able to use other lignin-derived compounds, such as 4-hydroxybenzoic acid, ferulic acid, syringic acid, and benzoic acid. Vanillic acid was detected in all culture media when isolates were grown on ferulic acid as the sole carbon source; however, no 4-hydroxy-3-methoxystyrene was detected, indicating that ferulic acid metabolism by these strains occurs via the elimination of two side chain carbons. Furthermore, the isolates exhibit 3,4-dioxygenase or 4,5-dioxygenase activity for protocatechuic acid ring-cleavage, which is consistent with the genetic sequences of related genera. This study was conducted to isolate and characterize marine bacteria of degrading lignin-derived compounds, thereby revealing the degradation of aromatic compounds in the marine environment and opening up new avenues for the development and utilization of marine biological resources.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33027312</pmid><doi>10.1371/journal.pone.0240187</doi><tpages>e0240187</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2020-10, Vol.15 (10), p.e0240187 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2449153789 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acids Aromatic compounds Arthrobacter Arthrobacter - metabolism Bacteria Bacterial Proteins - metabolism Benzoic acid Benzoic Acid - metabolism Biodegradation Biology and Life Sciences Biomarkers Biotransformation Carbon Carbon sources Cellulose Chemical analysis Chemical properties Computer and Information Sciences Coumaric Acids - metabolism Culture media Deoxyribonucleic acid Dioxygenase Dioxygenases - metabolism DNA Earth Sciences Ecology and Environmental Sciences Energy sources Environmental degradation Enzymes Ferulic acid Funding Gallic Acid - analogs & derivatives Gallic Acid - metabolism Gene sequencing Halomonas - metabolism Hydroxybenzoates - metabolism Industrial Microbiology - methods Life sciences Lignin Lignin - analogs & derivatives Lignin - metabolism Marine bacteria Marine biology Marine environment Marine resources Marinomonas - metabolism Metabolism Methods Microbiota Microorganisms Oceans Organic matter p-Hydroxybenzoic acid Phylogenetics Phylogeny Physical Sciences Physiological aspects Plants Protocatechuic acid Pseudoalteromonas - metabolism Seawater Seawater - microbiology Sediments Sodium chloride Strains (organisms) Terrestrial environments Vanillic acid Water analysis |
| title | Isolation and characterization marine bacteria capable of degrading lignin-derived compounds |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T16%3A11%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isolation%20and%20characterization%20marine%20bacteria%20capable%20of%20degrading%20lignin-derived%20compounds&rft.jtitle=PloS%20one&rft.au=Lu,%20Peng&rft.date=2020-10-07&rft.volume=15&rft.issue=10&rft.spage=e0240187&rft.pages=e0240187-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0240187&rft_dat=%3Cgale_plos_%3EA637699242%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2449153789&rft_id=info:pmid/33027312&rft_galeid=A637699242&rft_doaj_id=oai_doaj_org_article_3d0692cf38cc4a68b85b0aaf0d9ca3f8&rfr_iscdi=true |