Fine mapping of an anthracnose-resistance locus in Andean common bean cultivar Amendoim Cavalo
Anthracnose, caused by the fungal pathogen Colletotrichum lindemuthianum, is one of the world's most destructive diseases of common bean. The use of resistant cultivars is the most cost-effective strategy to manage this disease; however, durable resistance is difficult to achieve due to the vas...
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| creator | Gilio, Thiago Alexandre Santana Hurtado-Gonzales, Oscar P Gonçalves-Vidigal, Maria Celeste Valentini, Giseli Ferreira Elias, Julio Cesar Song, Qijian Pastor-Corrales, Marcial A |
| description | Anthracnose, caused by the fungal pathogen Colletotrichum lindemuthianum, is one of the world's most destructive diseases of common bean. The use of resistant cultivars is the most cost-effective strategy to manage this disease; however, durable resistance is difficult to achieve due to the vast virulence diversity of the anthracnose pathogen. Finding new genes with broad-spectrum resistance increases the prospect of designing an effective anthracnose-management strategy. Genetic analysis confirmed the presence of a single, dominant anthracnose-resistance locus in AC, which we provisionally named Co-AC. Bulk segregant analysis and genetic mapping of two F2 populations from the crosses AC × PI207262 and AC × G 2333 were used to determine the position of the Co-AC locus in a 631 Kbp genomic region flanked by the SNP markers SS56 and SS92 on the lower arm of chromosome Pv01. By genotyping 77 F3 plants from the AC × PI207262 cross using nine additional markers, we fine-mapped the Co-AC locus to a significantly smaller genomic region (9.4 Kbp) flanked by the SNP markers SS102 and SS165. This 9.4 Kbp region harbors three predicted genes based on the common bean reference genome, notably including the gene model Phvul.001G244300, which encodes Clathrin heavy chain 1, a protein that supports specific stomatal regulation functions and might play a role in plant defense signaling. Because the Co-AC resistance locus is linked in cis, it can be selected with great efficiency using molecular markers. These results will be very useful for breeding programs aimed at developing bean cultivars with anthracnose resistance using marker-assisted selection. This study revealed the broad-spectrum resistance of AC to C. lindemuthianum and the existence of the Co-AC anthracnose-resistance locus. Fine mapping positioned this locus in a small genomic region on the lower end of chromosome Pv01 that contained three candidate genes for the Co-AC locus. |
| doi_str_mv | 10.1371/journal.pone.0239763 |
| format | Article |
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The use of resistant cultivars is the most cost-effective strategy to manage this disease; however, durable resistance is difficult to achieve due to the vast virulence diversity of the anthracnose pathogen. Finding new genes with broad-spectrum resistance increases the prospect of designing an effective anthracnose-management strategy. Genetic analysis confirmed the presence of a single, dominant anthracnose-resistance locus in AC, which we provisionally named Co-AC. Bulk segregant analysis and genetic mapping of two F2 populations from the crosses AC × PI207262 and AC × G 2333 were used to determine the position of the Co-AC locus in a 631 Kbp genomic region flanked by the SNP markers SS56 and SS92 on the lower arm of chromosome Pv01. By genotyping 77 F3 plants from the AC × PI207262 cross using nine additional markers, we fine-mapped the Co-AC locus to a significantly smaller genomic region (9.4 Kbp) flanked by the SNP markers SS102 and SS165. This 9.4 Kbp region harbors three predicted genes based on the common bean reference genome, notably including the gene model Phvul.001G244300, which encodes Clathrin heavy chain 1, a protein that supports specific stomatal regulation functions and might play a role in plant defense signaling. Because the Co-AC resistance locus is linked in cis, it can be selected with great efficiency using molecular markers. These results will be very useful for breeding programs aimed at developing bean cultivars with anthracnose resistance using marker-assisted selection. This study revealed the broad-spectrum resistance of AC to C. lindemuthianum and the existence of the Co-AC anthracnose-resistance locus. Fine mapping positioned this locus in a small genomic region on the lower end of chromosome Pv01 that contained three candidate genes for the Co-AC locus.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0239763</identifier><identifier>PMID: 33027258</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agricultural research ; Anthracnose ; Beans ; Biology and Life Sciences ; Breeding ; Breeding - methods ; Chromosome Mapping - methods ; Chromosomes ; Clathrin ; Colletotrichum - pathogenicity ; Common beans ; Cultivars ; Disease resistance ; Disease resistance (Plants) ; Disease Resistance - genetics ; Diseases and pests ; Funding ; Gene mapping ; Genes ; Genes, Plant - genetics ; Genetic analysis ; Genetic aspects ; Genetic Linkage - genetics ; Genetic Markers - genetics ; Genomes ; Genomics ; Genotype ; Genotyping ; Laboratories ; Loci ; Marker-assisted selection ; Markers ; Pathogens ; Phaseolus - genetics ; Phaseolus - microbiology ; Plant breeding ; Plant Diseases - genetics ; Plant Diseases - microbiology ; Polymorphism, Single Nucleotide - genetics ; Population ; Research and Analysis Methods ; Seeds ; Single-nucleotide polymorphism ; Soybeans ; Stomata ; Virulence</subject><ispartof>PloS one, 2020-10, Vol.15 (10), p.e0239763-e0239763</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication: https://creativecommons.org/publicdomain/zero/1.0/ (the “License”). 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This 9.4 Kbp region harbors three predicted genes based on the common bean reference genome, notably including the gene model Phvul.001G244300, which encodes Clathrin heavy chain 1, a protein that supports specific stomatal regulation functions and might play a role in plant defense signaling. Because the Co-AC resistance locus is linked in cis, it can be selected with great efficiency using molecular markers. These results will be very useful for breeding programs aimed at developing bean cultivars with anthracnose resistance using marker-assisted selection. This study revealed the broad-spectrum resistance of AC to C. lindemuthianum and the existence of the Co-AC anthracnose-resistance locus. Fine mapping positioned this locus in a small genomic region on the lower end of chromosome Pv01 that contained three candidate genes for the Co-AC locus.</description><subject>Agricultural research</subject><subject>Anthracnose</subject><subject>Beans</subject><subject>Biology and Life Sciences</subject><subject>Breeding</subject><subject>Breeding - methods</subject><subject>Chromosome Mapping - methods</subject><subject>Chromosomes</subject><subject>Clathrin</subject><subject>Colletotrichum - pathogenicity</subject><subject>Common beans</subject><subject>Cultivars</subject><subject>Disease resistance</subject><subject>Disease resistance (Plants)</subject><subject>Disease Resistance - genetics</subject><subject>Diseases and pests</subject><subject>Funding</subject><subject>Gene mapping</subject><subject>Genes</subject><subject>Genes, Plant - genetics</subject><subject>Genetic analysis</subject><subject>Genetic aspects</subject><subject>Genetic Linkage - genetics</subject><subject>Genetic Markers - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gilio, Thiago Alexandre Santana</au><au>Hurtado-Gonzales, Oscar P</au><au>Gonçalves-Vidigal, Maria Celeste</au><au>Valentini, Giseli</au><au>Ferreira Elias, Julio Cesar</au><au>Song, Qijian</au><au>Pastor-Corrales, Marcial A</au><au>Mir, Reyazul Rouf</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fine mapping of an anthracnose-resistance locus in Andean common bean cultivar Amendoim Cavalo</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2020-10-07</date><risdate>2020</risdate><volume>15</volume><issue>10</issue><spage>e0239763</spage><epage>e0239763</epage><pages>e0239763-e0239763</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Anthracnose, caused by the fungal pathogen Colletotrichum lindemuthianum, is one of the world's most destructive diseases of common bean. The use of resistant cultivars is the most cost-effective strategy to manage this disease; however, durable resistance is difficult to achieve due to the vast virulence diversity of the anthracnose pathogen. Finding new genes with broad-spectrum resistance increases the prospect of designing an effective anthracnose-management strategy. Genetic analysis confirmed the presence of a single, dominant anthracnose-resistance locus in AC, which we provisionally named Co-AC. Bulk segregant analysis and genetic mapping of two F2 populations from the crosses AC × PI207262 and AC × G 2333 were used to determine the position of the Co-AC locus in a 631 Kbp genomic region flanked by the SNP markers SS56 and SS92 on the lower arm of chromosome Pv01. By genotyping 77 F3 plants from the AC × PI207262 cross using nine additional markers, we fine-mapped the Co-AC locus to a significantly smaller genomic region (9.4 Kbp) flanked by the SNP markers SS102 and SS165. This 9.4 Kbp region harbors three predicted genes based on the common bean reference genome, notably including the gene model Phvul.001G244300, which encodes Clathrin heavy chain 1, a protein that supports specific stomatal regulation functions and might play a role in plant defense signaling. Because the Co-AC resistance locus is linked in cis, it can be selected with great efficiency using molecular markers. These results will be very useful for breeding programs aimed at developing bean cultivars with anthracnose resistance using marker-assisted selection. This study revealed the broad-spectrum resistance of AC to C. lindemuthianum and the existence of the Co-AC anthracnose-resistance locus. Fine mapping positioned this locus in a small genomic region on the lower end of chromosome Pv01 that contained three candidate genes for the Co-AC locus.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33027258</pmid><doi>10.1371/journal.pone.0239763</doi><tpages>e0239763</tpages><orcidid>https://orcid.org/0000-0003-4078-5407</orcidid><oa>free_for_read</oa></addata></record> |
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| language | eng |
| recordid | cdi_plos_journals_2449153752 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Agricultural research Anthracnose Beans Biology and Life Sciences Breeding Breeding - methods Chromosome Mapping - methods Chromosomes Clathrin Colletotrichum - pathogenicity Common beans Cultivars Disease resistance Disease resistance (Plants) Disease Resistance - genetics Diseases and pests Funding Gene mapping Genes Genes, Plant - genetics Genetic analysis Genetic aspects Genetic Linkage - genetics Genetic Markers - genetics Genomes Genomics Genotype Genotyping Laboratories Loci Marker-assisted selection Markers Pathogens Phaseolus - genetics Phaseolus - microbiology Plant breeding Plant Diseases - genetics Plant Diseases - microbiology Polymorphism, Single Nucleotide - genetics Population Research and Analysis Methods Seeds Single-nucleotide polymorphism Soybeans Stomata Virulence |
| title | Fine mapping of an anthracnose-resistance locus in Andean common bean cultivar Amendoim Cavalo |
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