Polydopamine-assisted PDGF-BB immobilization on PLGA fibrous substrate enhances wound healing via regulating anti-inflammatory and cytokine secretion
Platelet-derived growth factor-bb (PDGF-BB) is a potent chemokine and mitogen for fibroblasts, keratinocytes, and vascular endothelium in the injured area, believed to be effective in wound healing. However, the short half-life of PDGF-BB and its rapid release from the wound surface limited its effi...
Gespeichert in:
| Veröffentlicht in: | PloS one 2020-09, Vol.15 (9), p.e0239366-e0239366 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0239366 |
|---|---|
| container_issue | 9 |
| container_start_page | e0239366 |
| container_title | PloS one |
| container_volume | 15 |
| creator | Yang, Xiao Zhan, Peng Wang, Xiuyan Zhang, Qiushuang Zhang, Yi Fan, Haitao Li, Ranwei Zhang, Ming |
| description | Platelet-derived growth factor-bb (PDGF-BB) is a potent chemokine and mitogen for fibroblasts, keratinocytes, and vascular endothelium in the injured area, believed to be effective in wound healing. However, the short half-life of PDGF-BB and its rapid release from the wound surface limited its efficacy in vivo and vitro. To evaluate the wound healing effects of dorsal skin in SD rats with polydopamine-assisted immobilized PDGF-BB on PLGA nanofibrous substrate. First, the effects of pDA-coating and PDGF-BB immobilization on the morphology, compositions, and hydrophilicity of substrates were evaluated in details. Second, the wound healing effect of pDA/PLGA/PDGF-BB substrate was assessed in the dorsal skin of SD rats. Last, the cytokine analysis by ELISA method was employed to evaluate the advantages of pDA/PLGA/PDGF-BB substrate on anti-inflammatory, angiogenesis, and cellular proliferation. This method significantly improved the immobilization amount and stability of PDGF-BB on the substrate (p |
| doi_str_mv | 10.1371/journal.pone.0239366 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2447247195</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A636883554</galeid><doaj_id>oai_doaj_org_article_588daf3b181c434eb8d014551c5c1d76</doaj_id><sourcerecordid>A636883554</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-ad6c9b4429a341d7c8256f7a48b580a15f1760fcfeebf472385ee850d670afbf3</originalsourceid><addsrcrecordid>eNqNk9tu1DAQhiMEoqXwBggiISG4yBLHhzg3lbaFLpUqteJ0azmOvevi2IvtFJb34H1xumnVRb1AiZRk8v0z4zlk2XNQzgCswbtLN3jLzWztrJyVFWwgIQ-yfdDAqiBVCR_eed_LnoRwWZYYUkIeZ3uwahqAm2Y_-3PhzKZza95rKwsegg5RdvnF-8VJcXSU6753rTb6N4_a2TzdF2eLea50690Q8jC0IXoeZS7tilshQ_7TDbbLV5IbbZf5lea5l8vBJH365DbqQltleN_z6PwmWbpcbKL7nsLnQQovx0BPs0eKmyCfTc-D7OvJhy_HH4uz88Xp8fysEKSpYsE7IpoWoarhEIGuFrTCRNUc0RbTkgOsQE1KJZSUrUJ1BSmWkuKyI3XJVavgQfZy63dtXGBTRQOrUIJRDRqciNMt0Tl-ydZe99xvmOOaXRucXzLuoxZGMkxpxxVsAQUCQSRb2pUAYQwEFik5knwdTtGGtpedkDbVzuw43f1j9Yot3RWrcWovGZN5Mznw7scgQ2S9DkIaw61M7bjOGyNa0Tqhr_5B7z_dRC15OkBqjEtxxeiUzQkklEKMUaJm91Dp6mSvRRo_pZN9R_B2R5CYKH_FJR9CYKefP_0_e_5tl319hx1nLK6CM8M4MmEXRFtQeBeCl-q2yKBk4_bcVION28Om7UmyF3cbdCu6WRf4F6QRF9w</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2447247195</pqid></control><display><type>article</type><title>Polydopamine-assisted PDGF-BB immobilization on PLGA fibrous substrate enhances wound healing via regulating anti-inflammatory and cytokine secretion</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Yang, Xiao ; Zhan, Peng ; Wang, Xiuyan ; Zhang, Qiushuang ; Zhang, Yi ; Fan, Haitao ; Li, Ranwei ; Zhang, Ming</creator><contributor>Caporali, Andrea</contributor><creatorcontrib>Yang, Xiao ; Zhan, Peng ; Wang, Xiuyan ; Zhang, Qiushuang ; Zhang, Yi ; Fan, Haitao ; Li, Ranwei ; Zhang, Ming ; Caporali, Andrea</creatorcontrib><description><![CDATA[Platelet-derived growth factor-bb (PDGF-BB) is a potent chemokine and mitogen for fibroblasts, keratinocytes, and vascular endothelium in the injured area, believed to be effective in wound healing. However, the short half-life of PDGF-BB and its rapid release from the wound surface limited its efficacy in vivo and vitro. To evaluate the wound healing effects of dorsal skin in SD rats with polydopamine-assisted immobilized PDGF-BB on PLGA nanofibrous substrate. First, the effects of pDA-coating and PDGF-BB immobilization on the morphology, compositions, and hydrophilicity of substrates were evaluated in details. Second, the wound healing effect of pDA/PLGA/PDGF-BB substrate was assessed in the dorsal skin of SD rats. Last, the cytokine analysis by ELISA method was employed to evaluate the advantages of pDA/PLGA/PDGF-BB substrate on anti-inflammatory, angiogenesis, and cellular proliferation. This method significantly improved the immobilization amount and stability of PDGF-BB on the substrate (p<0.01), further improved the hydrophilicity of substrates (p<0.05). Furthermore, the wound closure process was much more accelerated in the pDA/PLGA/PDGF-BB group (p<0.05). H&E and CD31 staining informed that the wound treated by pDA/PLGA/PDGF-BB substrate showed a high degree of regeneration and angiogenesis. The cytokine analysis showed that pDA significantly reduced the high level of inflammatory cytokines such as TNF-α (p<0.05). And the immobilized PDGF-BB significantly elevated the level of TGF-β and VEGF (p<0.05). The pDA/PLGA/PDGF-BB substrate showed great therapeutic effect on wound healing compared with other control groups via regulating anti-inflammatory, angiogenesis, and cellular proliferation. Absolutely, this report offered an available novel method for skin regeneration.]]></description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0239366</identifier><identifier>PMID: 32991599</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Angiogenesis ; Animals ; Anti-Inflammatory Agents - chemistry ; Anti-Inflammatory Agents - pharmacology ; Becaplermin - chemistry ; Becaplermin - pharmacology ; Biology and Life Sciences ; Cell adhesion & migration ; Chemokines ; Copolymers ; Cytokines ; Cytokines - metabolism ; Diabetes ; Dopamine ; Endothelium ; Engineering and Technology ; Equipment and supplies ; Evaluation ; Fibroblasts ; Growth factors ; Health aspects ; Hydrophilicity ; Immobilization ; Immobilized Proteins - chemistry ; Immobilized Proteins - pharmacology ; Indoles - chemistry ; Inflammation ; Keratinocytes ; Kinetics ; Male ; Medical research ; Medicine and Health Sciences ; Methods ; Morphology ; Nanomedicine ; Peptides ; Physical Sciences ; Platelet-derived growth factor ; Platelet-derived growth factor BB ; Polylactic Acid-Polyglycolic Acid Copolymer - chemistry ; Polylactide-co-glycolide ; Polymers ; Polymers - chemistry ; Rats ; Rats, Sprague-Dawley ; Regeneration ; Research and Analysis Methods ; Skin ; Substrates ; Tumor necrosis factor-α ; Ulcers ; Urology ; Vascular endothelial growth factor ; Wound care ; Wound healing ; Wound Healing - drug effects</subject><ispartof>PloS one, 2020-09, Vol.15 (9), p.e0239366-e0239366</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Yang et al 2020 Yang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-ad6c9b4429a341d7c8256f7a48b580a15f1760fcfeebf472385ee850d670afbf3</citedby><cites>FETCH-LOGICAL-c692t-ad6c9b4429a341d7c8256f7a48b580a15f1760fcfeebf472385ee850d670afbf3</cites><orcidid>0000-0002-4872-0651</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523965/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523965/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23868,27926,27927,53793,53795</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32991599$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Caporali, Andrea</contributor><creatorcontrib>Yang, Xiao</creatorcontrib><creatorcontrib>Zhan, Peng</creatorcontrib><creatorcontrib>Wang, Xiuyan</creatorcontrib><creatorcontrib>Zhang, Qiushuang</creatorcontrib><creatorcontrib>Zhang, Yi</creatorcontrib><creatorcontrib>Fan, Haitao</creatorcontrib><creatorcontrib>Li, Ranwei</creatorcontrib><creatorcontrib>Zhang, Ming</creatorcontrib><title>Polydopamine-assisted PDGF-BB immobilization on PLGA fibrous substrate enhances wound healing via regulating anti-inflammatory and cytokine secretion</title><title>PloS one</title><addtitle>PLoS One</addtitle><description><![CDATA[Platelet-derived growth factor-bb (PDGF-BB) is a potent chemokine and mitogen for fibroblasts, keratinocytes, and vascular endothelium in the injured area, believed to be effective in wound healing. However, the short half-life of PDGF-BB and its rapid release from the wound surface limited its efficacy in vivo and vitro. To evaluate the wound healing effects of dorsal skin in SD rats with polydopamine-assisted immobilized PDGF-BB on PLGA nanofibrous substrate. First, the effects of pDA-coating and PDGF-BB immobilization on the morphology, compositions, and hydrophilicity of substrates were evaluated in details. Second, the wound healing effect of pDA/PLGA/PDGF-BB substrate was assessed in the dorsal skin of SD rats. Last, the cytokine analysis by ELISA method was employed to evaluate the advantages of pDA/PLGA/PDGF-BB substrate on anti-inflammatory, angiogenesis, and cellular proliferation. This method significantly improved the immobilization amount and stability of PDGF-BB on the substrate (p<0.01), further improved the hydrophilicity of substrates (p<0.05). Furthermore, the wound closure process was much more accelerated in the pDA/PLGA/PDGF-BB group (p<0.05). H&E and CD31 staining informed that the wound treated by pDA/PLGA/PDGF-BB substrate showed a high degree of regeneration and angiogenesis. The cytokine analysis showed that pDA significantly reduced the high level of inflammatory cytokines such as TNF-α (p<0.05). And the immobilized PDGF-BB significantly elevated the level of TGF-β and VEGF (p<0.05). The pDA/PLGA/PDGF-BB substrate showed great therapeutic effect on wound healing compared with other control groups via regulating anti-inflammatory, angiogenesis, and cellular proliferation. Absolutely, this report offered an available novel method for skin regeneration.]]></description><subject>Angiogenesis</subject><subject>Animals</subject><subject>Anti-Inflammatory Agents - chemistry</subject><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>Becaplermin - chemistry</subject><subject>Becaplermin - pharmacology</subject><subject>Biology and Life Sciences</subject><subject>Cell adhesion & migration</subject><subject>Chemokines</subject><subject>Copolymers</subject><subject>Cytokines</subject><subject>Cytokines - metabolism</subject><subject>Diabetes</subject><subject>Dopamine</subject><subject>Endothelium</subject><subject>Engineering and Technology</subject><subject>Equipment and supplies</subject><subject>Evaluation</subject><subject>Fibroblasts</subject><subject>Growth factors</subject><subject>Health aspects</subject><subject>Hydrophilicity</subject><subject>Immobilization</subject><subject>Immobilized Proteins - chemistry</subject><subject>Immobilized Proteins - pharmacology</subject><subject>Indoles - chemistry</subject><subject>Inflammation</subject><subject>Keratinocytes</subject><subject>Kinetics</subject><subject>Male</subject><subject>Medical research</subject><subject>Medicine and Health Sciences</subject><subject>Methods</subject><subject>Morphology</subject><subject>Nanomedicine</subject><subject>Peptides</subject><subject>Physical Sciences</subject><subject>Platelet-derived growth factor</subject><subject>Platelet-derived growth factor BB</subject><subject>Polylactic Acid-Polyglycolic Acid Copolymer - chemistry</subject><subject>Polylactide-co-glycolide</subject><subject>Polymers</subject><subject>Polymers - chemistry</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Regeneration</subject><subject>Research and Analysis Methods</subject><subject>Skin</subject><subject>Substrates</subject><subject>Tumor necrosis factor-α</subject><subject>Ulcers</subject><subject>Urology</subject><subject>Vascular endothelial growth factor</subject><subject>Wound care</subject><subject>Wound healing</subject><subject>Wound Healing - drug effects</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tu1DAQhiMEoqXwBggiISG4yBLHhzg3lbaFLpUqteJ0azmOvevi2IvtFJb34H1xumnVRb1AiZRk8v0z4zlk2XNQzgCswbtLN3jLzWztrJyVFWwgIQ-yfdDAqiBVCR_eed_LnoRwWZYYUkIeZ3uwahqAm2Y_-3PhzKZza95rKwsegg5RdvnF-8VJcXSU6753rTb6N4_a2TzdF2eLea50690Q8jC0IXoeZS7tilshQ_7TDbbLV5IbbZf5lea5l8vBJH365DbqQltleN_z6PwmWbpcbKL7nsLnQQovx0BPs0eKmyCfTc-D7OvJhy_HH4uz88Xp8fysEKSpYsE7IpoWoarhEIGuFrTCRNUc0RbTkgOsQE1KJZSUrUJ1BSmWkuKyI3XJVavgQfZy63dtXGBTRQOrUIJRDRqciNMt0Tl-ydZe99xvmOOaXRucXzLuoxZGMkxpxxVsAQUCQSRb2pUAYQwEFik5knwdTtGGtpedkDbVzuw43f1j9Yot3RWrcWovGZN5Mznw7scgQ2S9DkIaw61M7bjOGyNa0Tqhr_5B7z_dRC15OkBqjEtxxeiUzQkklEKMUaJm91Dp6mSvRRo_pZN9R_B2R5CYKH_FJR9CYKefP_0_e_5tl319hx1nLK6CM8M4MmEXRFtQeBeCl-q2yKBk4_bcVION28Om7UmyF3cbdCu6WRf4F6QRF9w</recordid><startdate>20200929</startdate><enddate>20200929</enddate><creator>Yang, Xiao</creator><creator>Zhan, Peng</creator><creator>Wang, Xiuyan</creator><creator>Zhang, Qiushuang</creator><creator>Zhang, Yi</creator><creator>Fan, Haitao</creator><creator>Li, Ranwei</creator><creator>Zhang, Ming</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-4872-0651</orcidid></search><sort><creationdate>20200929</creationdate><title>Polydopamine-assisted PDGF-BB immobilization on PLGA fibrous substrate enhances wound healing via regulating anti-inflammatory and cytokine secretion</title><author>Yang, Xiao ; Zhan, Peng ; Wang, Xiuyan ; Zhang, Qiushuang ; Zhang, Yi ; Fan, Haitao ; Li, Ranwei ; Zhang, Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-ad6c9b4429a341d7c8256f7a48b580a15f1760fcfeebf472385ee850d670afbf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Angiogenesis</topic><topic>Animals</topic><topic>Anti-Inflammatory Agents - chemistry</topic><topic>Anti-Inflammatory Agents - pharmacology</topic><topic>Becaplermin - chemistry</topic><topic>Becaplermin - pharmacology</topic><topic>Biology and Life Sciences</topic><topic>Cell adhesion & migration</topic><topic>Chemokines</topic><topic>Copolymers</topic><topic>Cytokines</topic><topic>Cytokines - metabolism</topic><topic>Diabetes</topic><topic>Dopamine</topic><topic>Endothelium</topic><topic>Engineering and Technology</topic><topic>Equipment and supplies</topic><topic>Evaluation</topic><topic>Fibroblasts</topic><topic>Growth factors</topic><topic>Health aspects</topic><topic>Hydrophilicity</topic><topic>Immobilization</topic><topic>Immobilized Proteins - chemistry</topic><topic>Immobilized Proteins - pharmacology</topic><topic>Indoles - chemistry</topic><topic>Inflammation</topic><topic>Keratinocytes</topic><topic>Kinetics</topic><topic>Male</topic><topic>Medical research</topic><topic>Medicine and Health Sciences</topic><topic>Methods</topic><topic>Morphology</topic><topic>Nanomedicine</topic><topic>Peptides</topic><topic>Physical Sciences</topic><topic>Platelet-derived growth factor</topic><topic>Platelet-derived growth factor BB</topic><topic>Polylactic Acid-Polyglycolic Acid Copolymer - chemistry</topic><topic>Polylactide-co-glycolide</topic><topic>Polymers</topic><topic>Polymers - chemistry</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Regeneration</topic><topic>Research and Analysis Methods</topic><topic>Skin</topic><topic>Substrates</topic><topic>Tumor necrosis factor-α</topic><topic>Ulcers</topic><topic>Urology</topic><topic>Vascular endothelial growth factor</topic><topic>Wound care</topic><topic>Wound healing</topic><topic>Wound Healing - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Xiao</creatorcontrib><creatorcontrib>Zhan, Peng</creatorcontrib><creatorcontrib>Wang, Xiuyan</creatorcontrib><creatorcontrib>Zhang, Qiushuang</creatorcontrib><creatorcontrib>Zhang, Yi</creatorcontrib><creatorcontrib>Fan, Haitao</creatorcontrib><creatorcontrib>Li, Ranwei</creatorcontrib><creatorcontrib>Zhang, Ming</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Xiao</au><au>Zhan, Peng</au><au>Wang, Xiuyan</au><au>Zhang, Qiushuang</au><au>Zhang, Yi</au><au>Fan, Haitao</au><au>Li, Ranwei</au><au>Zhang, Ming</au><au>Caporali, Andrea</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polydopamine-assisted PDGF-BB immobilization on PLGA fibrous substrate enhances wound healing via regulating anti-inflammatory and cytokine secretion</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2020-09-29</date><risdate>2020</risdate><volume>15</volume><issue>9</issue><spage>e0239366</spage><epage>e0239366</epage><pages>e0239366-e0239366</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract><![CDATA[Platelet-derived growth factor-bb (PDGF-BB) is a potent chemokine and mitogen for fibroblasts, keratinocytes, and vascular endothelium in the injured area, believed to be effective in wound healing. However, the short half-life of PDGF-BB and its rapid release from the wound surface limited its efficacy in vivo and vitro. To evaluate the wound healing effects of dorsal skin in SD rats with polydopamine-assisted immobilized PDGF-BB on PLGA nanofibrous substrate. First, the effects of pDA-coating and PDGF-BB immobilization on the morphology, compositions, and hydrophilicity of substrates were evaluated in details. Second, the wound healing effect of pDA/PLGA/PDGF-BB substrate was assessed in the dorsal skin of SD rats. Last, the cytokine analysis by ELISA method was employed to evaluate the advantages of pDA/PLGA/PDGF-BB substrate on anti-inflammatory, angiogenesis, and cellular proliferation. This method significantly improved the immobilization amount and stability of PDGF-BB on the substrate (p<0.01), further improved the hydrophilicity of substrates (p<0.05). Furthermore, the wound closure process was much more accelerated in the pDA/PLGA/PDGF-BB group (p<0.05). H&E and CD31 staining informed that the wound treated by pDA/PLGA/PDGF-BB substrate showed a high degree of regeneration and angiogenesis. The cytokine analysis showed that pDA significantly reduced the high level of inflammatory cytokines such as TNF-α (p<0.05). And the immobilized PDGF-BB significantly elevated the level of TGF-β and VEGF (p<0.05). The pDA/PLGA/PDGF-BB substrate showed great therapeutic effect on wound healing compared with other control groups via regulating anti-inflammatory, angiogenesis, and cellular proliferation. Absolutely, this report offered an available novel method for skin regeneration.]]></abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32991599</pmid><doi>10.1371/journal.pone.0239366</doi><tpages>e0239366</tpages><orcidid>https://orcid.org/0000-0002-4872-0651</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2020-09, Vol.15 (9), p.e0239366-e0239366 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2447247195 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Angiogenesis Animals Anti-Inflammatory Agents - chemistry Anti-Inflammatory Agents - pharmacology Becaplermin - chemistry Becaplermin - pharmacology Biology and Life Sciences Cell adhesion & migration Chemokines Copolymers Cytokines Cytokines - metabolism Diabetes Dopamine Endothelium Engineering and Technology Equipment and supplies Evaluation Fibroblasts Growth factors Health aspects Hydrophilicity Immobilization Immobilized Proteins - chemistry Immobilized Proteins - pharmacology Indoles - chemistry Inflammation Keratinocytes Kinetics Male Medical research Medicine and Health Sciences Methods Morphology Nanomedicine Peptides Physical Sciences Platelet-derived growth factor Platelet-derived growth factor BB Polylactic Acid-Polyglycolic Acid Copolymer - chemistry Polylactide-co-glycolide Polymers Polymers - chemistry Rats Rats, Sprague-Dawley Regeneration Research and Analysis Methods Skin Substrates Tumor necrosis factor-α Ulcers Urology Vascular endothelial growth factor Wound care Wound healing Wound Healing - drug effects |
| title | Polydopamine-assisted PDGF-BB immobilization on PLGA fibrous substrate enhances wound healing via regulating anti-inflammatory and cytokine secretion |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T12%3A20%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Polydopamine-assisted%20PDGF-BB%20immobilization%20on%20PLGA%20fibrous%20substrate%20enhances%20wound%20healing%20via%20regulating%20anti-inflammatory%20and%20cytokine%20secretion&rft.jtitle=PloS%20one&rft.au=Yang,%20Xiao&rft.date=2020-09-29&rft.volume=15&rft.issue=9&rft.spage=e0239366&rft.epage=e0239366&rft.pages=e0239366-e0239366&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0239366&rft_dat=%3Cgale_plos_%3EA636883554%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2447247195&rft_id=info:pmid/32991599&rft_galeid=A636883554&rft_doaj_id=oai_doaj_org_article_588daf3b181c434eb8d014551c5c1d76&rfr_iscdi=true |