Aquaporin expression in the alimentary canal of the honey bee Apis mellifera L. (Hymenoptera: Apidae) and functional characterization of Am_Eglp 1
Aquaporins (AQP) are a family of plasma membrane proteins responsible for water transport through cell membranes. They are differentially expressed in different parts of the alimentary canal of insects where they regulate water transport. These proteins have been studied in detail in some insects, b...
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| description | Aquaporins (AQP) are a family of plasma membrane proteins responsible for water transport through cell membranes. They are differentially expressed in different parts of the alimentary canal of insects where they regulate water transport. These proteins have been studied in detail in some insects, but few data are available for aquaporins of the honey bee, Apis mellifera. We used quantitative PCR to study the expression of six putative aquaporin genes in forager honey bees. We found differential expression of all putative AQP genes in crop, midgut, ileum, rectum and Malpighian tubules. We found the entomoglyceroporin Am_Eglp 1 expressed at extremely high levels in the midgut. We performed a functional characterization of Am_Eglp 1 using heterologous expression in Xenopus laevis oocyte followed by water uptake assays. Our results confirmed that the Am_Eglp 1 gene encodes a functional water transporter. This study shows that all putative honey bee aquaporin genes have complex expression patterns in the digestive and excretory organs of honey bee workers. Our results suggest that Am_Eglp 1 is the principal water transporter in the midgut of A. mellifera workers. |
| doi_str_mv | 10.1371/journal.pone.0236724 |
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These proteins have been studied in detail in some insects, but few data are available for aquaporins of the honey bee, Apis mellifera. We used quantitative PCR to study the expression of six putative aquaporin genes in forager honey bees. We found differential expression of all putative AQP genes in crop, midgut, ileum, rectum and Malpighian tubules. We found the entomoglyceroporin Am_Eglp 1 expressed at extremely high levels in the midgut. We performed a functional characterization of Am_Eglp 1 using heterologous expression in Xenopus laevis oocyte followed by water uptake assays. Our results confirmed that the Am_Eglp 1 gene encodes a functional water transporter. This study shows that all putative honey bee aquaporin genes have complex expression patterns in the digestive and excretory organs of honey bee workers. Our results suggest that Am_Eglp 1 is the principal water transporter in the midgut of A. mellifera workers.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0236724</identifier><identifier>PMID: 32956406</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amino acids ; Animals ; Apis mellifera ; Aquaporins ; Aquaporins - genetics ; Aquaporins - metabolism ; Bees ; Bees - genetics ; Bees - metabolism ; Biology and Life Sciences ; Cell membranes ; Digestive system ; European honeybee ; Gametocytes ; Gastrointestinal tract ; Gastrointestinal Tract - metabolism ; Gene expression ; Genes ; Genes, Insect ; Genetic aspects ; Genomes ; Honey ; Ileum ; Insect Proteins - genetics ; Insect Proteins - metabolism ; Insects ; Malpighian tubules ; Membrane proteins ; Midgut ; Molecular weight ; Oocytes ; Organs ; Permeability ; Physical Sciences ; Physiological aspects ; Protein transport ; Proteins ; Rectum ; Research and Analysis Methods ; Water transport ; Water uptake ; Workers (insect caste) ; Xenopus laevis</subject><ispartof>PloS one, 2020-09, Vol.15 (9), p.e0236724-e0236724</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Linhares Lino de Souza et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Our results suggest that Am_Eglp 1 is the principal water transporter in the midgut of A. mellifera workers.</description><subject>Amino acids</subject><subject>Animals</subject><subject>Apis mellifera</subject><subject>Aquaporins</subject><subject>Aquaporins - genetics</subject><subject>Aquaporins - metabolism</subject><subject>Bees</subject><subject>Bees - genetics</subject><subject>Bees - metabolism</subject><subject>Biology and Life Sciences</subject><subject>Cell membranes</subject><subject>Digestive system</subject><subject>European honeybee</subject><subject>Gametocytes</subject><subject>Gastrointestinal tract</subject><subject>Gastrointestinal Tract - metabolism</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genes, Insect</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Honey</subject><subject>Ileum</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - metabolism</subject><subject>Insects</subject><subject>Malpighian tubules</subject><subject>Membrane proteins</subject><subject>Midgut</subject><subject>Molecular weight</subject><subject>Oocytes</subject><subject>Organs</subject><subject>Permeability</subject><subject>Physical Sciences</subject><subject>Physiological aspects</subject><subject>Protein transport</subject><subject>Proteins</subject><subject>Rectum</subject><subject>Research and Analysis Methods</subject><subject>Water transport</subject><subject>Water uptake</subject><subject>Workers (insect caste)</subject><subject>Xenopus laevis</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUstu1DAUjRCIlsIfILDEpixm8DsOC6RRVWilkdjA2rrj2DOukji1E8TwGXwxTietOqjywvb1efge3aJ4S_CSsJJ8uglj7KBZ9qGzS0yZLCl_VpySitGFpJg9f3Q-KV6ldIOxYErKl8UJo5WQHMvT4u_qdoQ-RN8h-7uPNiUfOpRvw84iaHxruwHiHhnIXii4u_ouW-7Rxlq06n1CrW0a72wEtF6i86t95oR-yPfP03sN9iOCrkZu7MyQ1bOO2UEEkyH-D0ylSXjV6stt0yPyunjhoEn2zbyfFT-_Xv64uFqsv3-7vlitF0ZgxRdUUSCEClwpVZXYEleDqmu6oaAwq2jNMZNYGcZKU5HSKSMM5kQJZ7CiVrGz4v1Bt29C0nOcSVPOuahYpWRGXB8QdYAb3Uff5ih0AK_vCiFuNcTBm8bqkm4IUY4bseFcAlTcbVzFS6I42KqGrPVldhs3ra1NzjVCcyR6_NL5nd6GX7oUWHCJs8D5LBDD7WjToFufTI4eOhvGw79VySUTGfrhP-jT3c2oLeQGfOdC9jWTqF5JJgmWgk-2yydQedW29SYPgvO5fkTgB4KJIaVo3UOPBOtpcu8_o6fJ1fPkZtq7x_k8kO5Hlf0Dd8jqvg</recordid><startdate>20200921</startdate><enddate>20200921</enddate><creator>Linhares Lino de Souza, Débora</creator><creator>Serrão, Jose Eduardo</creator><creator>Hansen, Immo Alex</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-6560-2606</orcidid><orcidid>https://orcid.org/0000-0002-0477-4252</orcidid></search><sort><creationdate>20200921</creationdate><title>Aquaporin expression in the alimentary canal of the honey bee Apis mellifera L. 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We found the entomoglyceroporin Am_Eglp 1 expressed at extremely high levels in the midgut. We performed a functional characterization of Am_Eglp 1 using heterologous expression in Xenopus laevis oocyte followed by water uptake assays. Our results confirmed that the Am_Eglp 1 gene encodes a functional water transporter. This study shows that all putative honey bee aquaporin genes have complex expression patterns in the digestive and excretory organs of honey bee workers. Our results suggest that Am_Eglp 1 is the principal water transporter in the midgut of A. mellifera workers.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32956406</pmid><doi>10.1371/journal.pone.0236724</doi><orcidid>https://orcid.org/0000-0002-6560-2606</orcidid><orcidid>https://orcid.org/0000-0002-0477-4252</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Amino acids Animals Apis mellifera Aquaporins Aquaporins - genetics Aquaporins - metabolism Bees Bees - genetics Bees - metabolism Biology and Life Sciences Cell membranes Digestive system European honeybee Gametocytes Gastrointestinal tract Gastrointestinal Tract - metabolism Gene expression Genes Genes, Insect Genetic aspects Genomes Honey Ileum Insect Proteins - genetics Insect Proteins - metabolism Insects Malpighian tubules Membrane proteins Midgut Molecular weight Oocytes Organs Permeability Physical Sciences Physiological aspects Protein transport Proteins Rectum Research and Analysis Methods Water transport Water uptake Workers (insect caste) Xenopus laevis |
| title | Aquaporin expression in the alimentary canal of the honey bee Apis mellifera L. (Hymenoptera: Apidae) and functional characterization of Am_Eglp 1 |
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