Molecular species identification of bushmeat recovered from the Serengeti ecosystem in Tanzania
Bushmeat harvesting and consumption represents a potential risk for the spillover of endemic zoonotic pathogens, yet remains a common practice in many parts of the world. Given that the harvesting and selling of bushmeat is illegal in Tanzania and other parts of Africa, the supply chain is informal...
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creator | Schilling, Megan A Estes, Anna B Eblate, Ernest Martin, Andimile Rentsch, Dennis Katani, Robab Joseph, Asteria Kindoro, Fatuma Lyimo, Beatus Radzio-Basu, Jessica Cattadori, Isabella M Hudson, Peter J Kapur, Vivek Buza, Joram J Gwakisa, Paul S |
description | Bushmeat harvesting and consumption represents a potential risk for the spillover of endemic zoonotic pathogens, yet remains a common practice in many parts of the world. Given that the harvesting and selling of bushmeat is illegal in Tanzania and other parts of Africa, the supply chain is informal and may include hunters, whole-sellers, retailers, and individual resellers who typically sell bushmeat in small pieces. These pieces are often further processed, obscuring species-identifying morphological characteristics, contributing to incomplete or mistaken knowledge of species of origin and potentially confounding assessments of pathogen spillover risk and bushmeat offtake. The current investigation sought to identify the species of origin and assess the concordance between seller-reported and laboratory-confirmed species of origin of bushmeat harvested from in and around the Serengeti National Park in Tanzania. After obtaining necessary permits, the species of origin of a total of 151 bushmeat samples purchased from known intermediaries from 2016 to 2018 were characterized by PCR and sequence analysis of the cytochrome B (CytB) gene. Based on these sequence analyses, 30%, 95% Confidence Interval (CI: 24.4-38.6) of bushmeat samples were misidentified by sellers. Misreporting amongst the top five source species (wildebeest, buffalo, impala, zebra, and giraffe) ranged from 20% (CI: 11.4-33.2) for samples reported as wildebeest to 47% (CI: 22.2-72.7) for samples reported as zebra although there was no systematic bias in reporting. Our findings suggest that while misreporting errors are unlikely to confound wildlife offtake estimates for bushmeat consumption within the Serengeti ecosystem, the role of misreporting bias on the risk of spillover events of endemic zoonotic infections from bushmeat requires further investigation. |
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Given that the harvesting and selling of bushmeat is illegal in Tanzania and other parts of Africa, the supply chain is informal and may include hunters, whole-sellers, retailers, and individual resellers who typically sell bushmeat in small pieces. These pieces are often further processed, obscuring species-identifying morphological characteristics, contributing to incomplete or mistaken knowledge of species of origin and potentially confounding assessments of pathogen spillover risk and bushmeat offtake. The current investigation sought to identify the species of origin and assess the concordance between seller-reported and laboratory-confirmed species of origin of bushmeat harvested from in and around the Serengeti National Park in Tanzania. After obtaining necessary permits, the species of origin of a total of 151 bushmeat samples purchased from known intermediaries from 2016 to 2018 were characterized by PCR and sequence analysis of the cytochrome B (CytB) gene. Based on these sequence analyses, 30%, 95% Confidence Interval (CI: 24.4-38.6) of bushmeat samples were misidentified by sellers. Misreporting amongst the top five source species (wildebeest, buffalo, impala, zebra, and giraffe) ranged from 20% (CI: 11.4-33.2) for samples reported as wildebeest to 47% (CI: 22.2-72.7) for samples reported as zebra although there was no systematic bias in reporting. Our findings suggest that while misreporting errors are unlikely to confound wildlife offtake estimates for bushmeat consumption within the Serengeti ecosystem, the role of misreporting bias on the risk of spillover events of endemic zoonotic infections from bushmeat requires further investigation.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0237590</identifier><identifier>PMID: 32925949</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal sciences ; Animals ; Animals, Wild - genetics ; Bias ; Biodiversity ; Bioengineering ; Biology and Life Sciences ; Buffaloes - genetics ; Commerce ; Confidence intervals ; Consumption ; Cytochrome ; Cytochrome b ; Cytochromes ; Cytochromes b - genetics ; Ecology and Environmental Sciences ; Ecosystem ; Ecosystem biology ; Ecosystems ; Equidae - genetics ; Game meat ; Giraffes - genetics ; Health risks ; Humans ; Identification ; Identification and classification ; Laboratories ; Life sciences ; Mandela, Nelson ; Meat - supply & distribution ; Medicine and Health Sciences ; National parks ; Parks, Recreational ; Pathogens ; People and Places ; Physical characteristics ; Population ; Sequence analysis ; Species ; Supervision ; Supply chains ; Tanzania - epidemiology ; Wildlife ; Wildlife conservation ; Zoology ; Zoonoses ; Zoonoses - etiology</subject><ispartof>PloS one, 2020-09, Vol.15 (9), p.e0237590</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Schilling et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Given that the harvesting and selling of bushmeat is illegal in Tanzania and other parts of Africa, the supply chain is informal and may include hunters, whole-sellers, retailers, and individual resellers who typically sell bushmeat in small pieces. These pieces are often further processed, obscuring species-identifying morphological characteristics, contributing to incomplete or mistaken knowledge of species of origin and potentially confounding assessments of pathogen spillover risk and bushmeat offtake. The current investigation sought to identify the species of origin and assess the concordance between seller-reported and laboratory-confirmed species of origin of bushmeat harvested from in and around the Serengeti National Park in Tanzania. After obtaining necessary permits, the species of origin of a total of 151 bushmeat samples purchased from known intermediaries from 2016 to 2018 were characterized by PCR and sequence analysis of the cytochrome B (CytB) gene. 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Jessica</au><au>Cattadori, Isabella M</au><au>Hudson, Peter J</au><au>Kapur, Vivek</au><au>Buza, Joram J</au><au>Gwakisa, Paul S</au><au>Munderloh, Ulrike Gertrud</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular species identification of bushmeat recovered from the Serengeti ecosystem in Tanzania</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2020-09-14</date><risdate>2020</risdate><volume>15</volume><issue>9</issue><spage>e0237590</spage><pages>e0237590-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Bushmeat harvesting and consumption represents a potential risk for the spillover of endemic zoonotic pathogens, yet remains a common practice in many parts of the world. Given that the harvesting and selling of bushmeat is illegal in Tanzania and other parts of Africa, the supply chain is informal and may include hunters, whole-sellers, retailers, and individual resellers who typically sell bushmeat in small pieces. These pieces are often further processed, obscuring species-identifying morphological characteristics, contributing to incomplete or mistaken knowledge of species of origin and potentially confounding assessments of pathogen spillover risk and bushmeat offtake. The current investigation sought to identify the species of origin and assess the concordance between seller-reported and laboratory-confirmed species of origin of bushmeat harvested from in and around the Serengeti National Park in Tanzania. After obtaining necessary permits, the species of origin of a total of 151 bushmeat samples purchased from known intermediaries from 2016 to 2018 were characterized by PCR and sequence analysis of the cytochrome B (CytB) gene. Based on these sequence analyses, 30%, 95% Confidence Interval (CI: 24.4-38.6) of bushmeat samples were misidentified by sellers. Misreporting amongst the top five source species (wildebeest, buffalo, impala, zebra, and giraffe) ranged from 20% (CI: 11.4-33.2) for samples reported as wildebeest to 47% (CI: 22.2-72.7) for samples reported as zebra although there was no systematic bias in reporting. Our findings suggest that while misreporting errors are unlikely to confound wildlife offtake estimates for bushmeat consumption within the Serengeti ecosystem, the role of misreporting bias on the risk of spillover events of endemic zoonotic infections from bushmeat requires further investigation.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32925949</pmid><doi>10.1371/journal.pone.0237590</doi><tpages>e0237590</tpages><orcidid>https://orcid.org/0000-0003-0588-8005</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1932-6203 |
ispartof | PloS one, 2020-09, Vol.15 (9), p.e0237590 |
issn | 1932-6203 1932-6203 |
language | eng |
recordid | cdi_plos_journals_2442589078 |
source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
subjects | Animal sciences Animals Animals, Wild - genetics Bias Biodiversity Bioengineering Biology and Life Sciences Buffaloes - genetics Commerce Confidence intervals Consumption Cytochrome Cytochrome b Cytochromes Cytochromes b - genetics Ecology and Environmental Sciences Ecosystem Ecosystem biology Ecosystems Equidae - genetics Game meat Giraffes - genetics Health risks Humans Identification Identification and classification Laboratories Life sciences Mandela, Nelson Meat - supply & distribution Medicine and Health Sciences National parks Parks, Recreational Pathogens People and Places Physical characteristics Population Sequence analysis Species Supervision Supply chains Tanzania - epidemiology Wildlife Wildlife conservation Zoology Zoonoses Zoonoses - etiology |
title | Molecular species identification of bushmeat recovered from the Serengeti ecosystem in Tanzania |
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