Genetic analysis of Cryptozona siamensis (Stylommatophora, Ariophantidae) populations in Thailand using the mitochondrial 16S rRNA and COI sequences
Cryptozona siamensis, one of the most widespread land snails, is native to Thailand, and plays a key role as an agricultural pest and intermediate host for Angiostrongylus spp. However, its genetic diversity and population structure has not yet been investigated, and are poorly understood. Therefore...
Gespeichert in:
| Veröffentlicht in: | PloS one 2020-09, Vol.15 (9), p.e0239264-e0239264 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0239264 |
|---|---|
| container_issue | 9 |
| container_start_page | e0239264 |
| container_title | PloS one |
| container_volume | 15 |
| creator | Dumidae, Abdulhakam Janthu, Pichamon Subkrasae, Chanakan Pumidonming, Wilawan Dekumyoy, Paron Thanwisai, Aunchalee Vitta, Apichat |
| description | Cryptozona siamensis, one of the most widespread land snails, is native to Thailand, and plays a key role as an agricultural pest and intermediate host for Angiostrongylus spp. However, its genetic diversity and population structure has not yet been investigated, and are poorly understood. Therefore, a genetic analysis of the C. siamensis population in Thailand was conducted, based mitochondrial 16S rRNA (402 bp) and COI (602 bp) gene fragment sequences. Cryptozona siamensis randomly collected from 17 locations in four populations across Thailand, between May 2017 and July 2018. Fifty-eight snails were used to examine the phylogeny, genetic diversity, and genetic structure. The maximum likelihood tree based on the 16S rRNA and COI fragment sequences revealed two main clades. A total of 14 haplotypes with 44 nucleotide variable sites were found in the 16S rRNA sequences, while 14 haplotypes with 57 nucleotide variable sites were found in the COI sequences. The genetic diversity of C. siamensis in term of the number of haplotypes and haplotype diversity, was found to be high but the nucleotide diversity showed low levels of genetic differentiation for the COI sequence as also noted with the 16S rRNA sequence. The population genetic structure of C. siamensis revealed genetic difference in most populations in Thailand. However, low genetic difference in some populations may be due to high gene flow. This study provides novel insights into the basic molecular genetics of C. siamensis. |
| doi_str_mv | 10.1371/journal.pone.0239264 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2442589074</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A635428646</galeid><doaj_id>oai_doaj_org_article_1ce66befb0534d5e8ab2c698d4b3bfd8</doaj_id><sourcerecordid>A635428646</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4854-8e433db7b6db1e1e73a7cb4429bd7fa34524e39a8cbe01aa06d344ab7e3c38a33</originalsourceid><addsrcrecordid>eNptklGL1DAQx4so3nn6DQQDvpzgrm2TpumLsCx6LhweeOdzmCTT3RxtUpNWWD-HH9isW8WVIw8Jk__8ZubPZNnLIl8WtC7e3fspOOiWg3e4zEvalJw9ys6LhpYLXub08T_vs-xZjPd5XlHB-dPsjJZNWTWCnWc_r9DhaDWBxNpHG4lvyTrsh9H_8A5ItNCjO8Qvb8d95_seRj_sfIC3ZBVseoIbrQF8QwY_TB2M1rtIrCN3O7AdOEOmaN2WjDskvR293nlngoWOFPyWhC-fV-QgWt9sSMRvEzqN8Xn2pIUu4ov5vsi-fvxwt_60uL652qxX1wvNRMUWAhmlRtWKG1VggTWFWivGykaZugXKqpIhbUBohXkBkHNDGQNVI9VUAKUX2asjd-h8lLOhUZYJUYkmr1lSbI4K4-FeDsH2EPbSg5W_Az5sJYRkX4ey0Mi5wlYll5mpUIAqNW-EYYqq1ojEej9Xm1SPRqMbA3Qn0NMfZ3dy67_LmommqooEuJwBwSen4ih7GzV2yWX007FvwXJByyR9_Z_04elm1RbSANa1PtXVB6hccVolGmc8qZYPqNIx2Fudtq-1KX6SwI4JOvgYA7Z_ZyxyedjdP83Iw-7KeXfpL-ST5QQ</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2442589074</pqid></control><display><type>article</type><title>Genetic analysis of Cryptozona siamensis (Stylommatophora, Ariophantidae) populations in Thailand using the mitochondrial 16S rRNA and COI sequences</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Dumidae, Abdulhakam ; Janthu, Pichamon ; Subkrasae, Chanakan ; Pumidonming, Wilawan ; Dekumyoy, Paron ; Thanwisai, Aunchalee ; Vitta, Apichat</creator><creatorcontrib>Dumidae, Abdulhakam ; Janthu, Pichamon ; Subkrasae, Chanakan ; Pumidonming, Wilawan ; Dekumyoy, Paron ; Thanwisai, Aunchalee ; Vitta, Apichat</creatorcontrib><description>Cryptozona siamensis, one of the most widespread land snails, is native to Thailand, and plays a key role as an agricultural pest and intermediate host for Angiostrongylus spp. However, its genetic diversity and population structure has not yet been investigated, and are poorly understood. Therefore, a genetic analysis of the C. siamensis population in Thailand was conducted, based mitochondrial 16S rRNA (402 bp) and COI (602 bp) gene fragment sequences. Cryptozona siamensis randomly collected from 17 locations in four populations across Thailand, between May 2017 and July 2018. Fifty-eight snails were used to examine the phylogeny, genetic diversity, and genetic structure. The maximum likelihood tree based on the 16S rRNA and COI fragment sequences revealed two main clades. A total of 14 haplotypes with 44 nucleotide variable sites were found in the 16S rRNA sequences, while 14 haplotypes with 57 nucleotide variable sites were found in the COI sequences. The genetic diversity of C. siamensis in term of the number of haplotypes and haplotype diversity, was found to be high but the nucleotide diversity showed low levels of genetic differentiation for the COI sequence as also noted with the 16S rRNA sequence. The population genetic structure of C. siamensis revealed genetic difference in most populations in Thailand. However, low genetic difference in some populations may be due to high gene flow. This study provides novel insights into the basic molecular genetics of C. siamensis.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0239264</identifier><identifier>PMID: 32925984</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Angiostrongylus ; Ariophantidae ; Biodiversity ; Biology and Life Sciences ; Computer and Information Sciences ; Cryptozona siamensis ; Deoxyribonucleic acid ; DNA ; Earth Sciences ; Ecology and Environmental Sciences ; Gene flow ; Gene sequencing ; Genes ; Genetic analysis ; Genetic aspects ; Genetic diversity ; Genetic structure ; Genetics ; Haplotypes ; Identification and classification ; Methods ; Mitochondria ; Mitochondrial DNA ; Mollusks ; Morphology ; Nucleotides ; Parasitology ; People and Places ; Phylogeny ; Population ; Population genetics ; Population structure ; Populations ; RNA sequencing ; rRNA 16S ; Snails ; Stylommatophora ; Thermal cycling</subject><ispartof>PloS one, 2020-09, Vol.15 (9), p.e0239264-e0239264</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Dumidae et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Dumidae et al 2020 Dumidae et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4854-8e433db7b6db1e1e73a7cb4429bd7fa34524e39a8cbe01aa06d344ab7e3c38a33</citedby><cites>FETCH-LOGICAL-c4854-8e433db7b6db1e1e73a7cb4429bd7fa34524e39a8cbe01aa06d344ab7e3c38a33</cites><orcidid>0000-0002-2483-0100</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7489551/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7489551/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids></links><search><creatorcontrib>Dumidae, Abdulhakam</creatorcontrib><creatorcontrib>Janthu, Pichamon</creatorcontrib><creatorcontrib>Subkrasae, Chanakan</creatorcontrib><creatorcontrib>Pumidonming, Wilawan</creatorcontrib><creatorcontrib>Dekumyoy, Paron</creatorcontrib><creatorcontrib>Thanwisai, Aunchalee</creatorcontrib><creatorcontrib>Vitta, Apichat</creatorcontrib><title>Genetic analysis of Cryptozona siamensis (Stylommatophora, Ariophantidae) populations in Thailand using the mitochondrial 16S rRNA and COI sequences</title><title>PloS one</title><description>Cryptozona siamensis, one of the most widespread land snails, is native to Thailand, and plays a key role as an agricultural pest and intermediate host for Angiostrongylus spp. However, its genetic diversity and population structure has not yet been investigated, and are poorly understood. Therefore, a genetic analysis of the C. siamensis population in Thailand was conducted, based mitochondrial 16S rRNA (402 bp) and COI (602 bp) gene fragment sequences. Cryptozona siamensis randomly collected from 17 locations in four populations across Thailand, between May 2017 and July 2018. Fifty-eight snails were used to examine the phylogeny, genetic diversity, and genetic structure. The maximum likelihood tree based on the 16S rRNA and COI fragment sequences revealed two main clades. A total of 14 haplotypes with 44 nucleotide variable sites were found in the 16S rRNA sequences, while 14 haplotypes with 57 nucleotide variable sites were found in the COI sequences. The genetic diversity of C. siamensis in term of the number of haplotypes and haplotype diversity, was found to be high but the nucleotide diversity showed low levels of genetic differentiation for the COI sequence as also noted with the 16S rRNA sequence. The population genetic structure of C. siamensis revealed genetic difference in most populations in Thailand. However, low genetic difference in some populations may be due to high gene flow. This study provides novel insights into the basic molecular genetics of C. siamensis.</description><subject>Angiostrongylus</subject><subject>Ariophantidae</subject><subject>Biodiversity</subject><subject>Biology and Life Sciences</subject><subject>Computer and Information Sciences</subject><subject>Cryptozona siamensis</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Earth Sciences</subject><subject>Ecology and Environmental Sciences</subject><subject>Gene flow</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genetic analysis</subject><subject>Genetic aspects</subject><subject>Genetic diversity</subject><subject>Genetic structure</subject><subject>Genetics</subject><subject>Haplotypes</subject><subject>Identification and classification</subject><subject>Methods</subject><subject>Mitochondria</subject><subject>Mitochondrial DNA</subject><subject>Mollusks</subject><subject>Morphology</subject><subject>Nucleotides</subject><subject>Parasitology</subject><subject>People and Places</subject><subject>Phylogeny</subject><subject>Population</subject><subject>Population genetics</subject><subject>Population structure</subject><subject>Populations</subject><subject>RNA sequencing</subject><subject>rRNA 16S</subject><subject>Snails</subject><subject>Stylommatophora</subject><subject>Thermal cycling</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptklGL1DAQx4so3nn6DQQDvpzgrm2TpumLsCx6LhweeOdzmCTT3RxtUpNWWD-HH9isW8WVIw8Jk__8ZubPZNnLIl8WtC7e3fspOOiWg3e4zEvalJw9ys6LhpYLXub08T_vs-xZjPd5XlHB-dPsjJZNWTWCnWc_r9DhaDWBxNpHG4lvyTrsh9H_8A5ItNCjO8Qvb8d95_seRj_sfIC3ZBVseoIbrQF8QwY_TB2M1rtIrCN3O7AdOEOmaN2WjDskvR293nlngoWOFPyWhC-fV-QgWt9sSMRvEzqN8Xn2pIUu4ov5vsi-fvxwt_60uL652qxX1wvNRMUWAhmlRtWKG1VggTWFWivGykaZugXKqpIhbUBohXkBkHNDGQNVI9VUAKUX2asjd-h8lLOhUZYJUYkmr1lSbI4K4-FeDsH2EPbSg5W_Az5sJYRkX4ey0Mi5wlYll5mpUIAqNW-EYYqq1ojEej9Xm1SPRqMbA3Qn0NMfZ3dy67_LmommqooEuJwBwSen4ih7GzV2yWX007FvwXJByyR9_Z_04elm1RbSANa1PtXVB6hccVolGmc8qZYPqNIx2Fudtq-1KX6SwI4JOvgYA7Z_ZyxyedjdP83Iw-7KeXfpL-ST5QQ</recordid><startdate>20200914</startdate><enddate>20200914</enddate><creator>Dumidae, Abdulhakam</creator><creator>Janthu, Pichamon</creator><creator>Subkrasae, Chanakan</creator><creator>Pumidonming, Wilawan</creator><creator>Dekumyoy, Paron</creator><creator>Thanwisai, Aunchalee</creator><creator>Vitta, Apichat</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-2483-0100</orcidid></search><sort><creationdate>20200914</creationdate><title>Genetic analysis of Cryptozona siamensis (Stylommatophora, Ariophantidae) populations in Thailand using the mitochondrial 16S rRNA and COI sequences</title><author>Dumidae, Abdulhakam ; Janthu, Pichamon ; Subkrasae, Chanakan ; Pumidonming, Wilawan ; Dekumyoy, Paron ; Thanwisai, Aunchalee ; Vitta, Apichat</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4854-8e433db7b6db1e1e73a7cb4429bd7fa34524e39a8cbe01aa06d344ab7e3c38a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Angiostrongylus</topic><topic>Ariophantidae</topic><topic>Biodiversity</topic><topic>Biology and Life Sciences</topic><topic>Computer and Information Sciences</topic><topic>Cryptozona siamensis</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Earth Sciences</topic><topic>Ecology and Environmental Sciences</topic><topic>Gene flow</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genetic analysis</topic><topic>Genetic aspects</topic><topic>Genetic diversity</topic><topic>Genetic structure</topic><topic>Genetics</topic><topic>Haplotypes</topic><topic>Identification and classification</topic><topic>Methods</topic><topic>Mitochondria</topic><topic>Mitochondrial DNA</topic><topic>Mollusks</topic><topic>Morphology</topic><topic>Nucleotides</topic><topic>Parasitology</topic><topic>People and Places</topic><topic>Phylogeny</topic><topic>Population</topic><topic>Population genetics</topic><topic>Population structure</topic><topic>Populations</topic><topic>RNA sequencing</topic><topic>rRNA 16S</topic><topic>Snails</topic><topic>Stylommatophora</topic><topic>Thermal cycling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dumidae, Abdulhakam</creatorcontrib><creatorcontrib>Janthu, Pichamon</creatorcontrib><creatorcontrib>Subkrasae, Chanakan</creatorcontrib><creatorcontrib>Pumidonming, Wilawan</creatorcontrib><creatorcontrib>Dekumyoy, Paron</creatorcontrib><creatorcontrib>Thanwisai, Aunchalee</creatorcontrib><creatorcontrib>Vitta, Apichat</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dumidae, Abdulhakam</au><au>Janthu, Pichamon</au><au>Subkrasae, Chanakan</au><au>Pumidonming, Wilawan</au><au>Dekumyoy, Paron</au><au>Thanwisai, Aunchalee</au><au>Vitta, Apichat</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic analysis of Cryptozona siamensis (Stylommatophora, Ariophantidae) populations in Thailand using the mitochondrial 16S rRNA and COI sequences</atitle><jtitle>PloS one</jtitle><date>2020-09-14</date><risdate>2020</risdate><volume>15</volume><issue>9</issue><spage>e0239264</spage><epage>e0239264</epage><pages>e0239264-e0239264</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Cryptozona siamensis, one of the most widespread land snails, is native to Thailand, and plays a key role as an agricultural pest and intermediate host for Angiostrongylus spp. However, its genetic diversity and population structure has not yet been investigated, and are poorly understood. Therefore, a genetic analysis of the C. siamensis population in Thailand was conducted, based mitochondrial 16S rRNA (402 bp) and COI (602 bp) gene fragment sequences. Cryptozona siamensis randomly collected from 17 locations in four populations across Thailand, between May 2017 and July 2018. Fifty-eight snails were used to examine the phylogeny, genetic diversity, and genetic structure. The maximum likelihood tree based on the 16S rRNA and COI fragment sequences revealed two main clades. A total of 14 haplotypes with 44 nucleotide variable sites were found in the 16S rRNA sequences, while 14 haplotypes with 57 nucleotide variable sites were found in the COI sequences. The genetic diversity of C. siamensis in term of the number of haplotypes and haplotype diversity, was found to be high but the nucleotide diversity showed low levels of genetic differentiation for the COI sequence as also noted with the 16S rRNA sequence. The population genetic structure of C. siamensis revealed genetic difference in most populations in Thailand. However, low genetic difference in some populations may be due to high gene flow. This study provides novel insights into the basic molecular genetics of C. siamensis.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>32925984</pmid><doi>10.1371/journal.pone.0239264</doi><orcidid>https://orcid.org/0000-0002-2483-0100</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2020-09, Vol.15 (9), p.e0239264-e0239264 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2442589074 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Angiostrongylus Ariophantidae Biodiversity Biology and Life Sciences Computer and Information Sciences Cryptozona siamensis Deoxyribonucleic acid DNA Earth Sciences Ecology and Environmental Sciences Gene flow Gene sequencing Genes Genetic analysis Genetic aspects Genetic diversity Genetic structure Genetics Haplotypes Identification and classification Methods Mitochondria Mitochondrial DNA Mollusks Morphology Nucleotides Parasitology People and Places Phylogeny Population Population genetics Population structure Populations RNA sequencing rRNA 16S Snails Stylommatophora Thermal cycling |
| title | Genetic analysis of Cryptozona siamensis (Stylommatophora, Ariophantidae) populations in Thailand using the mitochondrial 16S rRNA and COI sequences |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T23%3A29%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic%20analysis%20of%20Cryptozona%20siamensis%20(Stylommatophora,%20Ariophantidae)%20populations%20in%20Thailand%20using%20the%20mitochondrial%2016S%20rRNA%20and%20COI%20sequences&rft.jtitle=PloS%20one&rft.au=Dumidae,%20Abdulhakam&rft.date=2020-09-14&rft.volume=15&rft.issue=9&rft.spage=e0239264&rft.epage=e0239264&rft.pages=e0239264-e0239264&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0239264&rft_dat=%3Cgale_plos_%3EA635428646%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2442589074&rft_id=info:pmid/32925984&rft_galeid=A635428646&rft_doaj_id=oai_doaj_org_article_1ce66befb0534d5e8ab2c698d4b3bfd8&rfr_iscdi=true |