Protective effects of stem cells from human exfoliated deciduous teeth derived conditioned medium on osteoarthritic chondrocytes
Treatment of osteoarthritis (OA) is still a major clinical challenge due to the limited inherent healing capacity of cartilage. Recent studies utilising stem cells suggest that the therapeutic benefits of these cells are mediated through the paracrine mechanism of bioactive molecules. The present st...
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| Veröffentlicht in: | PloS one 2020-09, Vol.15 (9), p.e0238449-e0238449 |
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| creator | Muhammad, Suleiman Alhaji Nordin, Norshariza Hussin, Paisal Mehat, Muhammad Zulfadli Abu Kasim, Noor Hayaty Fakurazi, Sharida Papaccio, Gianpaolo |
| description | Treatment of osteoarthritis (OA) is still a major clinical challenge due to the limited inherent healing capacity of cartilage. Recent studies utilising stem cells suggest that the therapeutic benefits of these cells are mediated through the paracrine mechanism of bioactive molecules. The present study evaluates the regenerative effect of stem cells from human exfoliated deciduous teeth (SHED) conditioned medium (CM) on OA chondrocytes. The CM was collected after the SHED were cultured in serum-free medium (SFM) for 48 or 72 h and the cells were characterised by the expression of MSC and pluripotency markers. Chondrocytes were stimulated with interleukin-1[beta] and treated with the CM. Subsequently, the expression of aggrecan, collagen type 2 (COL 2), matrix metalloproteinase-13 (MMP-13), nuclear factor-kB (NF-kB) and the level of inflammatory and anti-inflammatory markers were evaluated. SHED expressed mesenchymal stromal cell surface proteins but were negative for haematopoietic markers. SHED also showed protein expression of NANOG, OCT4 and SOX2 with differential subcellular localisation. Treatment of OA chondrocytes with CM enhanced anti-inflammation compared to control cells treated with SFM. Furthermore, the expression of MMP-13 and NF-kB was significantly downregulated in stimulated chondrocytes incubated in CM. The study also revealed that CM increased the expression of aggrecan and COL 2 in OA chondrocytes compared to SFM control. Both CM regenerate extracellular matrix proteins and mitigate increased MMP-13 expression through inhibition of NF-kB in OA chondrocytes due to the presence of bioactive molecules. The study underscores the potential of CM for OA treatment. |
| doi_str_mv | 10.1371/journal.pone.0238449 |
| format | Article |
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Recent studies utilising stem cells suggest that the therapeutic benefits of these cells are mediated through the paracrine mechanism of bioactive molecules. The present study evaluates the regenerative effect of stem cells from human exfoliated deciduous teeth (SHED) conditioned medium (CM) on OA chondrocytes. The CM was collected after the SHED were cultured in serum-free medium (SFM) for 48 or 72 h and the cells were characterised by the expression of MSC and pluripotency markers. Chondrocytes were stimulated with interleukin-1[beta] and treated with the CM. Subsequently, the expression of aggrecan, collagen type 2 (COL 2), matrix metalloproteinase-13 (MMP-13), nuclear factor-kB (NF-kB) and the level of inflammatory and anti-inflammatory markers were evaluated. SHED expressed mesenchymal stromal cell surface proteins but were negative for haematopoietic markers. SHED also showed protein expression of NANOG, OCT4 and SOX2 with differential subcellular localisation. Treatment of OA chondrocytes with CM enhanced anti-inflammation compared to control cells treated with SFM. Furthermore, the expression of MMP-13 and NF-kB was significantly downregulated in stimulated chondrocytes incubated in CM. The study also revealed that CM increased the expression of aggrecan and COL 2 in OA chondrocytes compared to SFM control. Both CM regenerate extracellular matrix proteins and mitigate increased MMP-13 expression through inhibition of NF-kB in OA chondrocytes due to the presence of bioactive molecules. The study underscores the potential of CM for OA treatment.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0238449</identifier><identifier>PMID: 32886713</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Aggrecan ; Biological activity ; Biology and Life Sciences ; Biomedical materials ; Care and treatment ; Cartilage ; Cartilage cells ; Cartilage diseases ; Cell surface ; Chondrocytes ; Collagen ; Collagenase 3 ; Deciduous teeth ; Dentistry ; Extracellular matrix ; Genotype & phenotype ; Hematopoietic stem cells ; IL-1β ; Inflammation ; Interleukins ; Markers ; Matrix metalloproteinase ; Matrix metalloproteinases ; Medicine and Health Sciences ; Mesenchymal stem cells ; Mesenchyme ; Metalloproteinase ; NF-κB protein ; Oct-4 protein ; Osteoarthritis ; Paracrine signalling ; Physiological aspects ; Pluripotency ; Proteins ; Serum-free medium ; Stem cell transplantation ; Stem cells</subject><ispartof>PloS one, 2020-09, Vol.15 (9), p.e0238449-e0238449</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Muhammad et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Recent studies utilising stem cells suggest that the therapeutic benefits of these cells are mediated through the paracrine mechanism of bioactive molecules. The present study evaluates the regenerative effect of stem cells from human exfoliated deciduous teeth (SHED) conditioned medium (CM) on OA chondrocytes. The CM was collected after the SHED were cultured in serum-free medium (SFM) for 48 or 72 h and the cells were characterised by the expression of MSC and pluripotency markers. Chondrocytes were stimulated with interleukin-1[beta] and treated with the CM. Subsequently, the expression of aggrecan, collagen type 2 (COL 2), matrix metalloproteinase-13 (MMP-13), nuclear factor-kB (NF-kB) and the level of inflammatory and anti-inflammatory markers were evaluated. SHED expressed mesenchymal stromal cell surface proteins but were negative for haematopoietic markers. SHED also showed protein expression of NANOG, OCT4 and SOX2 with differential subcellular localisation. Treatment of OA chondrocytes with CM enhanced anti-inflammation compared to control cells treated with SFM. Furthermore, the expression of MMP-13 and NF-kB was significantly downregulated in stimulated chondrocytes incubated in CM. The study also revealed that CM increased the expression of aggrecan and COL 2 in OA chondrocytes compared to SFM control. Both CM regenerate extracellular matrix proteins and mitigate increased MMP-13 expression through inhibition of NF-kB in OA chondrocytes due to the presence of bioactive molecules. 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effects of stem cells from human exfoliated deciduous teeth derived conditioned medium on osteoarthritic chondrocytes</title><author>Muhammad, Suleiman Alhaji ; Nordin, Norshariza ; Hussin, Paisal ; Mehat, Muhammad Zulfadli ; Abu Kasim, Noor Hayaty ; Fakurazi, Sharida ; Papaccio, Gianpaolo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c735t-e328bbe6f79803042d3e4f7aa14bc171c2c213d997490cdd35c2df2832ca2ac63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aggrecan</topic><topic>Biological activity</topic><topic>Biology and Life Sciences</topic><topic>Biomedical materials</topic><topic>Care and treatment</topic><topic>Cartilage</topic><topic>Cartilage cells</topic><topic>Cartilage diseases</topic><topic>Cell surface</topic><topic>Chondrocytes</topic><topic>Collagen</topic><topic>Collagenase 3</topic><topic>Deciduous teeth</topic><topic>Dentistry</topic><topic>Extracellular 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from human exfoliated deciduous teeth derived conditioned medium on osteoarthritic chondrocytes</atitle><jtitle>PloS one</jtitle><date>2020-09-04</date><risdate>2020</risdate><volume>15</volume><issue>9</issue><spage>e0238449</spage><epage>e0238449</epage><pages>e0238449-e0238449</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Treatment of osteoarthritis (OA) is still a major clinical challenge due to the limited inherent healing capacity of cartilage. Recent studies utilising stem cells suggest that the therapeutic benefits of these cells are mediated through the paracrine mechanism of bioactive molecules. The present study evaluates the regenerative effect of stem cells from human exfoliated deciduous teeth (SHED) conditioned medium (CM) on OA chondrocytes. The CM was collected after the SHED were cultured in serum-free medium (SFM) for 48 or 72 h and the cells were characterised by the expression of MSC and pluripotency markers. Chondrocytes were stimulated with interleukin-1[beta] and treated with the CM. Subsequently, the expression of aggrecan, collagen type 2 (COL 2), matrix metalloproteinase-13 (MMP-13), nuclear factor-kB (NF-kB) and the level of inflammatory and anti-inflammatory markers were evaluated. SHED expressed mesenchymal stromal cell surface proteins but were negative for haematopoietic markers. SHED also showed protein expression of NANOG, OCT4 and SOX2 with differential subcellular localisation. Treatment of OA chondrocytes with CM enhanced anti-inflammation compared to control cells treated with SFM. Furthermore, the expression of MMP-13 and NF-kB was significantly downregulated in stimulated chondrocytes incubated in CM. The study also revealed that CM increased the expression of aggrecan and COL 2 in OA chondrocytes compared to SFM control. Both CM regenerate extracellular matrix proteins and mitigate increased MMP-13 expression through inhibition of NF-kB in OA chondrocytes due to the presence of bioactive molecules. The study underscores the potential of CM for OA treatment.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>32886713</pmid><doi>10.1371/journal.pone.0238449</doi><tpages>e0238449</tpages><orcidid>https://orcid.org/0000-0002-9740-6458</orcidid><orcidid>https://orcid.org/0000-0003-2421-3556</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aggrecan Biological activity Biology and Life Sciences Biomedical materials Care and treatment Cartilage Cartilage cells Cartilage diseases Cell surface Chondrocytes Collagen Collagenase 3 Deciduous teeth Dentistry Extracellular matrix Genotype & phenotype Hematopoietic stem cells IL-1β Inflammation Interleukins Markers Matrix metalloproteinase Matrix metalloproteinases Medicine and Health Sciences Mesenchymal stem cells Mesenchyme Metalloproteinase NF-κB protein Oct-4 protein Osteoarthritis Paracrine signalling Physiological aspects Pluripotency Proteins Serum-free medium Stem cell transplantation Stem cells |
| title | Protective effects of stem cells from human exfoliated deciduous teeth derived conditioned medium on osteoarthritic chondrocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T05%3A24%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Protective%20effects%20of%20stem%20cells%20from%20human%20exfoliated%20deciduous%20teeth%20derived%20conditioned%20medium%20on%20osteoarthritic%20chondrocytes&rft.jtitle=PloS%20one&rft.au=Muhammad,%20Suleiman%20Alhaji&rft.date=2020-09-04&rft.volume=15&rft.issue=9&rft.spage=e0238449&rft.epage=e0238449&rft.pages=e0238449-e0238449&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0238449&rft_dat=%3Cgale_plos_%3EA634494189%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2440285687&rft_id=info:pmid/32886713&rft_galeid=A634494189&rft_doaj_id=oai_doaj_org_article_0ebae9011f4d4d3f8aeb91bc187316c8&rfr_iscdi=true |