Comparative study on three viral enrichment approaches based on RNA extraction for plant virus/viroid detection using high-throughput sequencing

High-throughput sequencing (HTS) has become increasingly popular as virus diagnostic tool. It has been used to detect and identify plant viruses and viroids in different types of matrices and tissues. A viral sequence enrichment method prior to HTS is required to increase the viral reads in the gene...

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Veröffentlicht in:	PloS one 2020-08, Vol.15 (8), p.e0237951-e0237951
Hauptverfasser:	Gaafar, Yahya Zakaria Abdou, Ziebell, Heiko
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies Biology and Life Sciences Comparative studies Cultivars Deoxyribonucleic acid Depletion Diagnosis Diagnostic software Diagnostic systems DNA DNA polymerase Double-stranded RNA EDTA Enrichment Epidemiology Flowers & plants Genetic aspects Genomes High-throughput screening (Biochemical assaying) Identification and classification Infections Insects Methods Next-generation sequencing Nucleotides Pathogens Plant diseases Plant extracts Plant viruses Research and analysis methods RNA sequencing RNA viruses rRNA Viroids Viruses
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