High genetic similarity between non-typhoidal Salmonella isolated from paired blood and stool samples of children in the Democratic Republic of the Congo
Non-typhoidal Salmonella (NTS) serotypes Typhimurium and Enteritidis are a major cause of bloodstream infections in children in sub-Saharan Africa but their reservoir is unknown. We compared pairs of NTS blood and stool isolates (with the same NTS serotype recovered in the same patient) for genetic...
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| creator | Phoba, Marie-France Barbé, Barbara Ley, Benedikt Van Puyvelde, Sandra Post, Annelies Mattheus, Wesley Deborggraeve, Stijn Lunguya, Octavie Jacobs, Jan |
| description | Non-typhoidal Salmonella (NTS) serotypes Typhimurium and Enteritidis are a major cause of bloodstream infections in children in sub-Saharan Africa but their reservoir is unknown. We compared pairs of NTS blood and stool isolates (with the same NTS serotype recovered in the same patient) for genetic similarity.
Between November 2013 and April 2017, hospital-admitted children (29 days to 14 years) with culture-confirmed NTS bloodstream infections were enrolled in a cross-sectional study at Kisantu Hospital, DR Congo. Stool cultures for Salmonella were performed on a subset of enrolled children, as well as on a control group of non-febrile hospital-admitted children. Pairs of blood and stool NTS isolates were assessed for genetic similarity by multiple-locus variable-number of tandem repeats (MLVA) and genomics analysis.
A total of 299 children with NTS grown from blood cultures (Typhimurium 68.6%, Enteritidis 30.4%, other NTS 1.0%) had a stool sample processed; in 105 (35.1%) of them NTS was detected (Typhimurium 70.5%, Enteritidis 25.7%, other NTS 3.8%). A total of 87/105 (82.9%) pairs of blood and stool NTS isolates were observed (representing 29.1% of the 299 children). Among 1598 controls, the proportion of NTS stool excretion was 2.1% (p < 0.0001). MLVA types among paired isolates were identical in 82/87 (94.3%) pairs (27.4% of the 299 children; 61/66 (92.4%) in Typhimurium and 21/21 (100%) in Enteritidis pairs). Genomics analysis confirmed high genetic similarity within 41/43 (95.3%) pairs, showing a median SNP difference of 1 (range 0-77) and 1 (range 0-4) for Typhimurium and Enteritidis pairs respectively. Typhimurium and Enteritidis isolates belonged to sequence types ST313 lineage II and ST11 respectively.
Nearly 30% of children with NTS bloodstream infection showed stool excretion of an NTS isolate with high genetic similarity, adding to the evidence of humans as a potential reservoir for NTS. |
| doi_str_mv | 10.1371/journal.pntd.0008377 |
| format | Article |
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Between November 2013 and April 2017, hospital-admitted children (29 days to 14 years) with culture-confirmed NTS bloodstream infections were enrolled in a cross-sectional study at Kisantu Hospital, DR Congo. Stool cultures for Salmonella were performed on a subset of enrolled children, as well as on a control group of non-febrile hospital-admitted children. Pairs of blood and stool NTS isolates were assessed for genetic similarity by multiple-locus variable-number of tandem repeats (MLVA) and genomics analysis.
A total of 299 children with NTS grown from blood cultures (Typhimurium 68.6%, Enteritidis 30.4%, other NTS 1.0%) had a stool sample processed; in 105 (35.1%) of them NTS was detected (Typhimurium 70.5%, Enteritidis 25.7%, other NTS 3.8%). A total of 87/105 (82.9%) pairs of blood and stool NTS isolates were observed (representing 29.1% of the 299 children). Among 1598 controls, the proportion of NTS stool excretion was 2.1% (p < 0.0001). MLVA types among paired isolates were identical in 82/87 (94.3%) pairs (27.4% of the 299 children; 61/66 (92.4%) in Typhimurium and 21/21 (100%) in Enteritidis pairs). Genomics analysis confirmed high genetic similarity within 41/43 (95.3%) pairs, showing a median SNP difference of 1 (range 0-77) and 1 (range 0-4) for Typhimurium and Enteritidis pairs respectively. Typhimurium and Enteritidis isolates belonged to sequence types ST313 lineage II and ST11 respectively.
Nearly 30% of children with NTS bloodstream infection showed stool excretion of an NTS isolate with high genetic similarity, adding to the evidence of humans as a potential reservoir for NTS.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0008377</identifier><identifier>PMID: 32614856</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Bacteremia ; Bacteremia - microbiology ; Biology and Life Sciences ; Blood ; Causes of ; Child ; Child, Preschool ; Children ; Computer and Information Sciences ; Cross-Sectional Studies ; Distribution ; Excretion ; Feces - microbiology ; Funding ; Genetic analysis ; Genetic aspects ; Genomes ; Genomics ; Health aspects ; Hospitals ; Host-bacteria relationships ; Humans ; Infant ; Infections ; Medicine ; Medicine and Health Sciences ; Microbiology ; Pediatric research ; Phylogenetics ; Polymorphism, Single Nucleotide ; Salmonella ; Salmonella - classification ; Salmonella - genetics ; Serotypes ; Similarity ; Single-nucleotide polymorphism ; Tropical diseases</subject><ispartof>PLoS neglected tropical diseases, 2020-07, Vol.14 (7), p.e0008377-e0008377</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Phoba et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Phoba et al 2020 Phoba et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c624t-f2d92eea111f9769246f63709513f493a97e4db371a7392c5b0d30876c7af0283</citedby><cites>FETCH-LOGICAL-c624t-f2d92eea111f9769246f63709513f493a97e4db371a7392c5b0d30876c7af0283</cites><orcidid>0000-0003-4978-3379 ; 0000-0001-8434-5732</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331982/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331982/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32614856$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Senok, Abiola</contributor><creatorcontrib>Phoba, Marie-France</creatorcontrib><creatorcontrib>Barbé, Barbara</creatorcontrib><creatorcontrib>Ley, Benedikt</creatorcontrib><creatorcontrib>Van Puyvelde, Sandra</creatorcontrib><creatorcontrib>Post, Annelies</creatorcontrib><creatorcontrib>Mattheus, Wesley</creatorcontrib><creatorcontrib>Deborggraeve, Stijn</creatorcontrib><creatorcontrib>Lunguya, Octavie</creatorcontrib><creatorcontrib>Jacobs, Jan</creatorcontrib><title>High genetic similarity between non-typhoidal Salmonella isolated from paired blood and stool samples of children in the Democratic Republic of the Congo</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>Non-typhoidal Salmonella (NTS) serotypes Typhimurium and Enteritidis are a major cause of bloodstream infections in children in sub-Saharan Africa but their reservoir is unknown. We compared pairs of NTS blood and stool isolates (with the same NTS serotype recovered in the same patient) for genetic similarity.
Between November 2013 and April 2017, hospital-admitted children (29 days to 14 years) with culture-confirmed NTS bloodstream infections were enrolled in a cross-sectional study at Kisantu Hospital, DR Congo. Stool cultures for Salmonella were performed on a subset of enrolled children, as well as on a control group of non-febrile hospital-admitted children. Pairs of blood and stool NTS isolates were assessed for genetic similarity by multiple-locus variable-number of tandem repeats (MLVA) and genomics analysis.
A total of 299 children with NTS grown from blood cultures (Typhimurium 68.6%, Enteritidis 30.4%, other NTS 1.0%) had a stool sample processed; in 105 (35.1%) of them NTS was detected (Typhimurium 70.5%, Enteritidis 25.7%, other NTS 3.8%). A total of 87/105 (82.9%) pairs of blood and stool NTS isolates were observed (representing 29.1% of the 299 children). Among 1598 controls, the proportion of NTS stool excretion was 2.1% (p < 0.0001). MLVA types among paired isolates were identical in 82/87 (94.3%) pairs (27.4% of the 299 children; 61/66 (92.4%) in Typhimurium and 21/21 (100%) in Enteritidis pairs). Genomics analysis confirmed high genetic similarity within 41/43 (95.3%) pairs, showing a median SNP difference of 1 (range 0-77) and 1 (range 0-4) for Typhimurium and Enteritidis pairs respectively. Typhimurium and Enteritidis isolates belonged to sequence types ST313 lineage II and ST11 respectively.
Nearly 30% of children with NTS bloodstream infection showed stool excretion of an NTS isolate with high genetic similarity, adding to the evidence of humans as a potential reservoir for NTS.</description><subject>Adolescent</subject><subject>Bacteremia</subject><subject>Bacteremia - microbiology</subject><subject>Biology and Life Sciences</subject><subject>Blood</subject><subject>Causes of</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Children</subject><subject>Computer and Information Sciences</subject><subject>Cross-Sectional Studies</subject><subject>Distribution</subject><subject>Excretion</subject><subject>Feces - microbiology</subject><subject>Funding</subject><subject>Genetic analysis</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Health aspects</subject><subject>Hospitals</subject><subject>Host-bacteria relationships</subject><subject>Humans</subject><subject>Infant</subject><subject>Infections</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Microbiology</subject><subject>Pediatric research</subject><subject>Phylogenetics</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Salmonella</subject><subject>Salmonella - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PLoS neglected tropical diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Phoba, Marie-France</au><au>Barbé, Barbara</au><au>Ley, Benedikt</au><au>Van Puyvelde, Sandra</au><au>Post, Annelies</au><au>Mattheus, Wesley</au><au>Deborggraeve, Stijn</au><au>Lunguya, Octavie</au><au>Jacobs, Jan</au><au>Senok, Abiola</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High genetic similarity between non-typhoidal Salmonella isolated from paired blood and stool samples of children in the Democratic Republic of the Congo</atitle><jtitle>PLoS neglected tropical diseases</jtitle><addtitle>PLoS Negl Trop Dis</addtitle><date>2020-07-01</date><risdate>2020</risdate><volume>14</volume><issue>7</issue><spage>e0008377</spage><epage>e0008377</epage><pages>e0008377-e0008377</pages><issn>1935-2735</issn><issn>1935-2727</issn><eissn>1935-2735</eissn><abstract>Non-typhoidal Salmonella (NTS) serotypes Typhimurium and Enteritidis are a major cause of bloodstream infections in children in sub-Saharan Africa but their reservoir is unknown. We compared pairs of NTS blood and stool isolates (with the same NTS serotype recovered in the same patient) for genetic similarity.
Between November 2013 and April 2017, hospital-admitted children (29 days to 14 years) with culture-confirmed NTS bloodstream infections were enrolled in a cross-sectional study at Kisantu Hospital, DR Congo. Stool cultures for Salmonella were performed on a subset of enrolled children, as well as on a control group of non-febrile hospital-admitted children. Pairs of blood and stool NTS isolates were assessed for genetic similarity by multiple-locus variable-number of tandem repeats (MLVA) and genomics analysis.
A total of 299 children with NTS grown from blood cultures (Typhimurium 68.6%, Enteritidis 30.4%, other NTS 1.0%) had a stool sample processed; in 105 (35.1%) of them NTS was detected (Typhimurium 70.5%, Enteritidis 25.7%, other NTS 3.8%). A total of 87/105 (82.9%) pairs of blood and stool NTS isolates were observed (representing 29.1% of the 299 children). Among 1598 controls, the proportion of NTS stool excretion was 2.1% (p < 0.0001). MLVA types among paired isolates were identical in 82/87 (94.3%) pairs (27.4% of the 299 children; 61/66 (92.4%) in Typhimurium and 21/21 (100%) in Enteritidis pairs). Genomics analysis confirmed high genetic similarity within 41/43 (95.3%) pairs, showing a median SNP difference of 1 (range 0-77) and 1 (range 0-4) for Typhimurium and Enteritidis pairs respectively. Typhimurium and Enteritidis isolates belonged to sequence types ST313 lineage II and ST11 respectively.
Nearly 30% of children with NTS bloodstream infection showed stool excretion of an NTS isolate with high genetic similarity, adding to the evidence of humans as a potential reservoir for NTS.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32614856</pmid><doi>10.1371/journal.pntd.0008377</doi><orcidid>https://orcid.org/0000-0003-4978-3379</orcidid><orcidid>https://orcid.org/0000-0001-8434-5732</orcidid><oa>free_for_read</oa></addata></record> |
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| issn | 1935-2735 1935-2727 1935-2735 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central Open Access; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Adolescent Bacteremia Bacteremia - microbiology Biology and Life Sciences Blood Causes of Child Child, Preschool Children Computer and Information Sciences Cross-Sectional Studies Distribution Excretion Feces - microbiology Funding Genetic analysis Genetic aspects Genomes Genomics Health aspects Hospitals Host-bacteria relationships Humans Infant Infections Medicine Medicine and Health Sciences Microbiology Pediatric research Phylogenetics Polymorphism, Single Nucleotide Salmonella Salmonella - classification Salmonella - genetics Serotypes Similarity Single-nucleotide polymorphism Tropical diseases |
| title | High genetic similarity between non-typhoidal Salmonella isolated from paired blood and stool samples of children in the Democratic Republic of the Congo |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T07%3A50%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=High%20genetic%20similarity%20between%20non-typhoidal%20Salmonella%20isolated%20from%20paired%20blood%20and%20stool%20samples%20of%20children%20in%20the%20Democratic%20Republic%20of%20the%20Congo&rft.jtitle=PLoS%20neglected%20tropical%20diseases&rft.au=Phoba,%20Marie-France&rft.date=2020-07-01&rft.volume=14&rft.issue=7&rft.spage=e0008377&rft.epage=e0008377&rft.pages=e0008377-e0008377&rft.issn=1935-2735&rft.eissn=1935-2735&rft_id=info:doi/10.1371/journal.pntd.0008377&rft_dat=%3Cgale_plos_%3EA632967982%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2434499627&rft_id=info:pmid/32614856&rft_galeid=A632967982&rft_doaj_id=oai_doaj_org_article_c2efb7a580fb4d4fa4ccd51a82958f28&rfr_iscdi=true |