High-fidelity SaCas9 identified by directional screening in human cells

CRISPR-Staphylococcus aureus Cas9 (CRISPR-SaCas9) has been harnessed as an effective in vivo genome-editing tool to manipulate genomes. However, off-target effects remain a major bottleneck that precludes safe and reliable applications in genome editing. Here, we characterize the off-target effects...

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Veröffentlicht in:	PLoS biology 2020-07, Vol.18 (7), p.e3000747-e3000747
Hauptverfasser:	Xie, Haihua, Ge, Xianglian, Yang, Fayu, Wang, Bang, Li, Shuang, Duan, Jinzhi, Lv, Xiujuan, Cheng, Congsheng, Song, Zongming, Liu, Changbao, Zhao, Junzhao, Zhang, Yu, Wu, Jinyu, Gao, Caixia, Zhang, Jinwei, Gu, Feng
Format:	Artikel
Sprache:	eng
Schlagworte:	Accuracy Bacterial Proteins - metabolism Biology and Life Sciences Cells CRISPR CRISPR-Associated Protein 9 - metabolism Deoxyribonucleic acid Diabetes DNA Editing Engineering and Technology Fidelity Funding Gene Library Genetic aspects Genetic Engineering Genetic Loci Genetic variation Genome editing Genome, Human Genomes Genomics HEK293 Cells Hospitals Humans Identification and classification Kidney diseases Laboratories Methods Methods and Resources Molecular biology Mutation Nucleotides Nucleotides - genetics Optometry Phenotype Reproducibility of Results Research and analysis methods Ribonucleic acid RNA Screening Software Staphylococcus aureus - metabolism Toolkits Transcriptional Activation - genetics
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