In vivo two-photon microscopic observation and ablation in deeper brain regions realized by modifications of excitation beam diameter and immersion liquid

In vivo two-photon microscopy utilizing a nonlinear optical process enables, in living mouse brains, not only the visualization of morphologies and functions of neural networks in deep regions but also their optical manipulation at targeted sites with high spatial precision. Because the two-photon e...

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Veröffentlicht in:	PloS one 2020-08, Vol.15 (8), p.e0237230-e0237230
Hauptverfasser:	Yamaguchi, Kazushi, Kitamura, Ryoji, Kawakami, Ryosuke, Otomo, Kohei, Nemoto, Tomomi
Format:	Artikel
Sprache:	eng
Schlagworte:	Ablation Astrocytes Biology and Life Sciences Brain cells Brain mapping Brain research Calcium Calcium (intracellular) Calcium signalling Dendrites Efficiency Engineering and Technology Excitation Experiments Glycerol Hippocampus Immersion Information science Laboratory animals Laser ablation Laser beams Laser scanning microscopy Lasers Light Medicine and Health Sciences Methods Microscopy Morphology Neural circuitry Neural networks Neurons Normal distribution Observations Photon density Photons Physical Sciences Physiology Pyramidal cells Refractive index Refractivity Research and Analysis Methods Software Submerging Visualization
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