Extracellular Vesicle cystatin c is associated with unstable angina in troponin negative patients with acute chest pain
Despite the use of high-sensitive cardiac troponin there remains a group of high-sensitive cardiac troponin negative patients with unstable angina with a non-neglectable risk for future adverse cardiovascular events, emphasising the need for additional risk stratification. Plasma extracellular vesic...
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| Veröffentlicht in: | PloS one 2020-08, Vol.15 (8), p.e0237036 |
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| creator | Dekker, Mirthe Waissi, Farahnaz van Bennekom, Joelle Silvis, Max J M Timmerman, Nathalie Schoneveld, Arjan H Grobbee, Diederick E de Winter, Robbert J Mosterd, Arend Timmers, Leo de Kleijn, Dominique P V |
| description | Despite the use of high-sensitive cardiac troponin there remains a group of high-sensitive cardiac troponin negative patients with unstable angina with a non-neglectable risk for future adverse cardiovascular events, emphasising the need for additional risk stratification. Plasma extracellular vesicles are small bilayer membrane vesicles known for their potential role as biomarker source. Their role in unstable angina remains unexplored. We investigate if extracellular vesicle proteins are associated with unstable angina in patients with chest pain and low high-sensitive cardiac troponin.
The MINERVA study included patients presenting with acute chest pain but no acute coronary syndrome. We performed an exploratory retrospective case-control analysis among 269 patients. Cases were defined as patients with low high-sensitive cardiac troponin and proven ischemia. Patients without ischemia were selected as controls. Blood samples were fractionated to analyse the EV proteins in three plasma-subfractions: TEX, HDL and LDL. Protein levels were quantified using electrochemiluminescence immunoassay.
Lower levels of (adjusted) EV cystatin c in the TEX subfraction were associated with having unstable angina (OR 0.93 95% CI 0.88-0.99).
In patients with acute chest pain but low high-sensitive cardiac troponin, lower levels of plasma extracellular vesicle cystatin c are associated with having unstable angina. This finding is hypothesis generating only considering the small sample size and needs to be confirmed in larger cohort studies, but still identifies extracellular vesicle proteins as source for additional risk stratification. |
| doi_str_mv | 10.1371/journal.pone.0237036 |
| format | Article |
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The MINERVA study included patients presenting with acute chest pain but no acute coronary syndrome. We performed an exploratory retrospective case-control analysis among 269 patients. Cases were defined as patients with low high-sensitive cardiac troponin and proven ischemia. Patients without ischemia were selected as controls. Blood samples were fractionated to analyse the EV proteins in three plasma-subfractions: TEX, HDL and LDL. Protein levels were quantified using electrochemiluminescence immunoassay.
Lower levels of (adjusted) EV cystatin c in the TEX subfraction were associated with having unstable angina (OR 0.93 95% CI 0.88-0.99).
In patients with acute chest pain but low high-sensitive cardiac troponin, lower levels of plasma extracellular vesicle cystatin c are associated with having unstable angina. This finding is hypothesis generating only considering the small sample size and needs to be confirmed in larger cohort studies, but still identifies extracellular vesicle proteins as source for additional risk stratification.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0237036</identifier><identifier>PMID: 32756583</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acute Coronary Syndrome - physiopathology ; Acute coronary syndromes ; Adult ; Aged ; Angina ; Angina pectoris ; Angina, Unstable - blood ; Angina, Unstable - metabolism ; Angina, Unstable - physiopathology ; Biology and Life Sciences ; Biomarkers ; Biomarkers - blood ; Calcium-binding protein ; Cardiology ; Cardiovascular diseases ; Case-Control Studies ; Chest ; Chest Pain - blood ; Chest Pain - metabolism ; Chest Pain - physiopathology ; Cohort Studies ; Creatine Kinase - blood ; Cystatin ; Cystatin C ; Cystatin C - analysis ; Cystatin C - blood ; Cystatin C - metabolism ; Diagnosis ; Electrocardiography ; Electrochemiluminescence ; Extracellular vesicles ; Extracellular Vesicles - metabolism ; Extracellular Vesicles - physiology ; Female ; Funding ; Health aspects ; Health risks ; Heart ; Heart attacks ; High density lipoprotein ; Humans ; Immunoassay ; Ischemia ; Lipids ; Lipoproteins (high density) ; Lipoproteins (low density) ; Low density lipoprotein ; Male ; Medicine and Health Sciences ; Membrane vesicles ; Middle Aged ; Myocardial Infarction - physiopathology ; Pain ; Pathophysiology ; Physiological aspects ; Plasma ; Proteins ; Retrospective Studies ; Risk ; Studies ; Troponin ; Troponin - blood ; Unstable angina ; Vascular surgery ; Vesicles</subject><ispartof>PloS one, 2020-08, Vol.15 (8), p.e0237036</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Dekker et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Dekker et al 2020 Dekker et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-a95bd4175fb4e5129cf890c2ee56a669153f08164c49908030d6b3bede4cb3053</citedby><cites>FETCH-LOGICAL-c692t-a95bd4175fb4e5129cf890c2ee56a669153f08164c49908030d6b3bede4cb3053</cites><orcidid>0000-0002-9336-9469 ; 0000-0002-6306-6096</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406038/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406038/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32756583$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>den Uil, Corstiaan</contributor><creatorcontrib>Dekker, Mirthe</creatorcontrib><creatorcontrib>Waissi, Farahnaz</creatorcontrib><creatorcontrib>van Bennekom, Joelle</creatorcontrib><creatorcontrib>Silvis, Max J M</creatorcontrib><creatorcontrib>Timmerman, Nathalie</creatorcontrib><creatorcontrib>Schoneveld, Arjan H</creatorcontrib><creatorcontrib>Grobbee, Diederick E</creatorcontrib><creatorcontrib>de Winter, Robbert J</creatorcontrib><creatorcontrib>Mosterd, Arend</creatorcontrib><creatorcontrib>Timmers, Leo</creatorcontrib><creatorcontrib>de Kleijn, Dominique P V</creatorcontrib><title>Extracellular Vesicle cystatin c is associated with unstable angina in troponin negative patients with acute chest pain</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Despite the use of high-sensitive cardiac troponin there remains a group of high-sensitive cardiac troponin negative patients with unstable angina with a non-neglectable risk for future adverse cardiovascular events, emphasising the need for additional risk stratification. Plasma extracellular vesicles are small bilayer membrane vesicles known for their potential role as biomarker source. Their role in unstable angina remains unexplored. We investigate if extracellular vesicle proteins are associated with unstable angina in patients with chest pain and low high-sensitive cardiac troponin.
The MINERVA study included patients presenting with acute chest pain but no acute coronary syndrome. We performed an exploratory retrospective case-control analysis among 269 patients. Cases were defined as patients with low high-sensitive cardiac troponin and proven ischemia. Patients without ischemia were selected as controls. Blood samples were fractionated to analyse the EV proteins in three plasma-subfractions: TEX, HDL and LDL. Protein levels were quantified using electrochemiluminescence immunoassay.
Lower levels of (adjusted) EV cystatin c in the TEX subfraction were associated with having unstable angina (OR 0.93 95% CI 0.88-0.99).
In patients with acute chest pain but low high-sensitive cardiac troponin, lower levels of plasma extracellular vesicle cystatin c are associated with having unstable angina. This finding is hypothesis generating only considering the small sample size and needs to be confirmed in larger cohort studies, but still identifies extracellular vesicle proteins as source for additional risk stratification.</description><subject>Acute Coronary Syndrome - physiopathology</subject><subject>Acute coronary syndromes</subject><subject>Adult</subject><subject>Aged</subject><subject>Angina</subject><subject>Angina pectoris</subject><subject>Angina, Unstable - blood</subject><subject>Angina, Unstable - metabolism</subject><subject>Angina, Unstable - physiopathology</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Biomarkers - blood</subject><subject>Calcium-binding protein</subject><subject>Cardiology</subject><subject>Cardiovascular diseases</subject><subject>Case-Control Studies</subject><subject>Chest</subject><subject>Chest Pain - blood</subject><subject>Chest Pain - metabolism</subject><subject>Chest Pain - physiopathology</subject><subject>Cohort Studies</subject><subject>Creatine Kinase - blood</subject><subject>Cystatin</subject><subject>Cystatin C</subject><subject>Cystatin C - analysis</subject><subject>Cystatin C - blood</subject><subject>Cystatin C - metabolism</subject><subject>Diagnosis</subject><subject>Electrocardiography</subject><subject>Electrochemiluminescence</subject><subject>Extracellular vesicles</subject><subject>Extracellular Vesicles - metabolism</subject><subject>Extracellular Vesicles - physiology</subject><subject>Female</subject><subject>Funding</subject><subject>Health aspects</subject><subject>Health risks</subject><subject>Heart</subject><subject>Heart attacks</subject><subject>High density lipoprotein</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Ischemia</subject><subject>Lipids</subject><subject>Lipoproteins (high density)</subject><subject>Lipoproteins (low density)</subject><subject>Low density lipoprotein</subject><subject>Male</subject><subject>Medicine and Health Sciences</subject><subject>Membrane vesicles</subject><subject>Middle Aged</subject><subject>Myocardial Infarction - 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physiopathology</topic><topic>Acute coronary syndromes</topic><topic>Adult</topic><topic>Aged</topic><topic>Angina</topic><topic>Angina pectoris</topic><topic>Angina, Unstable - blood</topic><topic>Angina, Unstable - metabolism</topic><topic>Angina, Unstable - physiopathology</topic><topic>Biology and Life Sciences</topic><topic>Biomarkers</topic><topic>Biomarkers - blood</topic><topic>Calcium-binding protein</topic><topic>Cardiology</topic><topic>Cardiovascular diseases</topic><topic>Case-Control Studies</topic><topic>Chest</topic><topic>Chest Pain - blood</topic><topic>Chest Pain - metabolism</topic><topic>Chest Pain - physiopathology</topic><topic>Cohort Studies</topic><topic>Creatine Kinase - blood</topic><topic>Cystatin</topic><topic>Cystatin C</topic><topic>Cystatin C - analysis</topic><topic>Cystatin C - blood</topic><topic>Cystatin C - metabolism</topic><topic>Diagnosis</topic><topic>Electrocardiography</topic><topic>Electrochemiluminescence</topic><topic>Extracellular vesicles</topic><topic>Extracellular Vesicles - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dekker, Mirthe</au><au>Waissi, Farahnaz</au><au>van Bennekom, Joelle</au><au>Silvis, Max J M</au><au>Timmerman, Nathalie</au><au>Schoneveld, Arjan H</au><au>Grobbee, Diederick E</au><au>de Winter, Robbert J</au><au>Mosterd, Arend</au><au>Timmers, Leo</au><au>de Kleijn, Dominique P V</au><au>den Uil, Corstiaan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extracellular Vesicle cystatin c is associated with unstable angina in troponin negative patients with acute chest pain</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2020-08-05</date><risdate>2020</risdate><volume>15</volume><issue>8</issue><spage>e0237036</spage><pages>e0237036-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Despite the use of high-sensitive cardiac troponin there remains a group of high-sensitive cardiac troponin negative patients with unstable angina with a non-neglectable risk for future adverse cardiovascular events, emphasising the need for additional risk stratification. Plasma extracellular vesicles are small bilayer membrane vesicles known for their potential role as biomarker source. Their role in unstable angina remains unexplored. We investigate if extracellular vesicle proteins are associated with unstable angina in patients with chest pain and low high-sensitive cardiac troponin.
The MINERVA study included patients presenting with acute chest pain but no acute coronary syndrome. We performed an exploratory retrospective case-control analysis among 269 patients. Cases were defined as patients with low high-sensitive cardiac troponin and proven ischemia. Patients without ischemia were selected as controls. Blood samples were fractionated to analyse the EV proteins in three plasma-subfractions: TEX, HDL and LDL. Protein levels were quantified using electrochemiluminescence immunoassay.
Lower levels of (adjusted) EV cystatin c in the TEX subfraction were associated with having unstable angina (OR 0.93 95% CI 0.88-0.99).
In patients with acute chest pain but low high-sensitive cardiac troponin, lower levels of plasma extracellular vesicle cystatin c are associated with having unstable angina. This finding is hypothesis generating only considering the small sample size and needs to be confirmed in larger cohort studies, but still identifies extracellular vesicle proteins as source for additional risk stratification.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32756583</pmid><doi>10.1371/journal.pone.0237036</doi><tpages>e0237036</tpages><orcidid>https://orcid.org/0000-0002-9336-9469</orcidid><orcidid>https://orcid.org/0000-0002-6306-6096</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2020-08, Vol.15 (8), p.e0237036 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2430658345 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acute Coronary Syndrome - physiopathology Acute coronary syndromes Adult Aged Angina Angina pectoris Angina, Unstable - blood Angina, Unstable - metabolism Angina, Unstable - physiopathology Biology and Life Sciences Biomarkers Biomarkers - blood Calcium-binding protein Cardiology Cardiovascular diseases Case-Control Studies Chest Chest Pain - blood Chest Pain - metabolism Chest Pain - physiopathology Cohort Studies Creatine Kinase - blood Cystatin Cystatin C Cystatin C - analysis Cystatin C - blood Cystatin C - metabolism Diagnosis Electrocardiography Electrochemiluminescence Extracellular vesicles Extracellular Vesicles - metabolism Extracellular Vesicles - physiology Female Funding Health aspects Health risks Heart Heart attacks High density lipoprotein Humans Immunoassay Ischemia Lipids Lipoproteins (high density) Lipoproteins (low density) Low density lipoprotein Male Medicine and Health Sciences Membrane vesicles Middle Aged Myocardial Infarction - physiopathology Pain Pathophysiology Physiological aspects Plasma Proteins Retrospective Studies Risk Studies Troponin Troponin - blood Unstable angina Vascular surgery Vesicles |
| title | Extracellular Vesicle cystatin c is associated with unstable angina in troponin negative patients with acute chest pain |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T02%3A17%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Extracellular%20Vesicle%20cystatin%20c%20is%20associated%20with%20unstable%20angina%20in%20troponin%20negative%20patients%20with%20acute%20chest%20pain&rft.jtitle=PloS%20one&rft.au=Dekker,%20Mirthe&rft.date=2020-08-05&rft.volume=15&rft.issue=8&rft.spage=e0237036&rft.pages=e0237036-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0237036&rft_dat=%3Cgale_plos_%3EA631637453%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2430658345&rft_id=info:pmid/32756583&rft_galeid=A631637453&rft_doaj_id=oai_doaj_org_article_383929d67bbf4807b02097add5e6b0d9&rfr_iscdi=true |