Roux-en-Y gastric bypass surgery changes fungal and bacterial microbiota in morbidly obese patients—A pilot study
The Roux-en-Y gastric bypass (RYGB) remains the most effective treatment for morbidly obese patients to lower body weight and improve glycemic control. There is recent evidence that the mycobiome (fungal microbiome) can aggravate disease severity in a number of diseases including inflammatory bowel...
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| creator | Steinert, Robert E Rehman, Ateequr Souto Lima, Everton Job Agamennone, Valeria Schuren, Frank H. J Gero, Daniel Schreiner, Phillip Vonlanthen, René Ismaeil, Aiman Tzafos, Stefanos Hosa, Hanna Vetter, Diana Misselwitz, Benjamin Bueter, Marco |
| description | The Roux-en-Y gastric bypass (RYGB) remains the most effective treatment for morbidly obese patients to lower body weight and improve glycemic control. There is recent evidence that the mycobiome (fungal microbiome) can aggravate disease severity in a number of diseases including inflammatory bowel disease (IBD), irritable bowel syndrome (IBS) and hepatitis; moreover, a dysbiotic fungal microbiota has been reported in the obese. We characterized fungal and bacterial microbial composition in fecal samples of 16 morbidly obese patients before and three months after RYGB surgery and compared with nine healthy controls. We found that RYGB surgery induced a clear alteration in structure and composition of the gut fungal and bacterial microbiota. Beta diversity analysis revealed significant differences in bacterial microbiota between obese patients before surgery and healthy controls (P < 0.005) and a significant, unidirectional shift in RYGB patients after surgery (P < 0.001 vs. before surgery). In contrast, there was no significant difference in fungal microbiota between groups but individually specific changes after RYGB surgery. Interestingly, RYGB surgery induced a significant reduction in fungal alpha diversity namely Chao1, Sobs, and Shannon diversity index (P |
| doi_str_mv | 10.1371/journal.pone.0236936 |
| format | Article |
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J ; Gero, Daniel ; Schreiner, Phillip ; Vonlanthen, René ; Ismaeil, Aiman ; Tzafos, Stefanos ; Hosa, Hanna ; Vetter, Diana ; Misselwitz, Benjamin ; Bueter, Marco</creator><creatorcontrib>Steinert, Robert E ; Rehman, Ateequr ; Souto Lima, Everton Job ; Agamennone, Valeria ; Schuren, Frank H. J ; Gero, Daniel ; Schreiner, Phillip ; Vonlanthen, René ; Ismaeil, Aiman ; Tzafos, Stefanos ; Hosa, Hanna ; Vetter, Diana ; Misselwitz, Benjamin ; Bueter, Marco</creatorcontrib><description>The Roux-en-Y gastric bypass (RYGB) remains the most effective treatment for morbidly obese patients to lower body weight and improve glycemic control. There is recent evidence that the mycobiome (fungal microbiome) can aggravate disease severity in a number of diseases including inflammatory bowel disease (IBD), irritable bowel syndrome (IBS) and hepatitis; moreover, a dysbiotic fungal microbiota has been reported in the obese. We characterized fungal and bacterial microbial composition in fecal samples of 16 morbidly obese patients before and three months after RYGB surgery and compared with nine healthy controls. We found that RYGB surgery induced a clear alteration in structure and composition of the gut fungal and bacterial microbiota. Beta diversity analysis revealed significant differences in bacterial microbiota between obese patients before surgery and healthy controls (P < 0.005) and a significant, unidirectional shift in RYGB patients after surgery (P < 0.001 vs. before surgery). In contrast, there was no significant difference in fungal microbiota between groups but individually specific changes after RYGB surgery. Interestingly, RYGB surgery induced a significant reduction in fungal alpha diversity namely Chao1, Sobs, and Shannon diversity index (P<0.05, respectively) which contrasts the trend for uniform changes in bacteria towards increased richness and diversity post-surgery. We did not observe any inter-kingdom relations in RYGB patients but in the healthy control cohort and there were several correlations between fungi and bacteria and clinical parameters (P<0.05, respectively) that warrant further research. Our study identifies changes in intestinal fungal communities in RYGB patients that are distinct to changes in the bacterial microbiota.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0236936</identifier><identifier>PMID: 32735609</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Bacteria ; Biology ; Biology and Life Sciences ; Body weight ; Care and treatment ; Composition ; Deoxyribonucleic acid ; DNA ; Feces ; Fungi ; Gastric bypass ; Gastrointestinal surgery ; Genetic testing ; Hepatitis ; Hospitals ; Inflammatory bowel diseases ; Intestine ; Irritable bowel syndrome ; Medicine and Health Sciences ; Metabolism ; Methods ; Microbiomes ; Microbiota ; Microbiota (Symbiotic organisms) ; Microorganisms ; Obesity ; Observations ; Open source software ; Parameter identification ; Patient outcomes ; Patients ; Statistical analysis ; Surgery ; Symbiosis ; Taxonomy ; Variance analysis ; Veganism</subject><ispartof>PloS one, 2020-07, Vol.15 (7), p.e0236936-e0236936</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Steinert et al. 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J</creatorcontrib><creatorcontrib>Gero, Daniel</creatorcontrib><creatorcontrib>Schreiner, Phillip</creatorcontrib><creatorcontrib>Vonlanthen, René</creatorcontrib><creatorcontrib>Ismaeil, Aiman</creatorcontrib><creatorcontrib>Tzafos, Stefanos</creatorcontrib><creatorcontrib>Hosa, Hanna</creatorcontrib><creatorcontrib>Vetter, Diana</creatorcontrib><creatorcontrib>Misselwitz, Benjamin</creatorcontrib><creatorcontrib>Bueter, Marco</creatorcontrib><title>Roux-en-Y gastric bypass surgery changes fungal and bacterial microbiota in morbidly obese patients—A pilot study</title><title>PloS one</title><description>The Roux-en-Y gastric bypass (RYGB) remains the most effective treatment for morbidly obese patients to lower body weight and improve glycemic control. 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Interestingly, RYGB surgery induced a significant reduction in fungal alpha diversity namely Chao1, Sobs, and Shannon diversity index (P<0.05, respectively) which contrasts the trend for uniform changes in bacteria towards increased richness and diversity post-surgery. We did not observe any inter-kingdom relations in RYGB patients but in the healthy control cohort and there were several correlations between fungi and bacteria and clinical parameters (P<0.05, respectively) that warrant further research. 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J</au><au>Gero, Daniel</au><au>Schreiner, Phillip</au><au>Vonlanthen, René</au><au>Ismaeil, Aiman</au><au>Tzafos, Stefanos</au><au>Hosa, Hanna</au><au>Vetter, Diana</au><au>Misselwitz, Benjamin</au><au>Bueter, Marco</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Roux-en-Y gastric bypass surgery changes fungal and bacterial microbiota in morbidly obese patients—A pilot study</atitle><jtitle>PloS one</jtitle><date>2020-07-31</date><risdate>2020</risdate><volume>15</volume><issue>7</issue><spage>e0236936</spage><epage>e0236936</epage><pages>e0236936-e0236936</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The Roux-en-Y gastric bypass (RYGB) remains the most effective treatment for morbidly obese patients to lower body weight and improve glycemic control. There is recent evidence that the mycobiome (fungal microbiome) can aggravate disease severity in a number of diseases including inflammatory bowel disease (IBD), irritable bowel syndrome (IBS) and hepatitis; moreover, a dysbiotic fungal microbiota has been reported in the obese. We characterized fungal and bacterial microbial composition in fecal samples of 16 morbidly obese patients before and three months after RYGB surgery and compared with nine healthy controls. We found that RYGB surgery induced a clear alteration in structure and composition of the gut fungal and bacterial microbiota. Beta diversity analysis revealed significant differences in bacterial microbiota between obese patients before surgery and healthy controls (P < 0.005) and a significant, unidirectional shift in RYGB patients after surgery (P < 0.001 vs. before surgery). In contrast, there was no significant difference in fungal microbiota between groups but individually specific changes after RYGB surgery. Interestingly, RYGB surgery induced a significant reduction in fungal alpha diversity namely Chao1, Sobs, and Shannon diversity index (P<0.05, respectively) which contrasts the trend for uniform changes in bacteria towards increased richness and diversity post-surgery. We did not observe any inter-kingdom relations in RYGB patients but in the healthy control cohort and there were several correlations between fungi and bacteria and clinical parameters (P<0.05, respectively) that warrant further research. Our study identifies changes in intestinal fungal communities in RYGB patients that are distinct to changes in the bacterial microbiota.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>32735609</pmid><doi>10.1371/journal.pone.0236936</doi><tpages>e0236936</tpages><orcidid>https://orcid.org/0000-0003-2941-9801</orcidid><orcidid>https://orcid.org/0000-0001-7884-5036</orcidid><oa>free_for_read</oa></addata></record> |
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| issn | 1932-6203 1932-6203 |
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| subjects | Bacteria Biology Biology and Life Sciences Body weight Care and treatment Composition Deoxyribonucleic acid DNA Feces Fungi Gastric bypass Gastrointestinal surgery Genetic testing Hepatitis Hospitals Inflammatory bowel diseases Intestine Irritable bowel syndrome Medicine and Health Sciences Metabolism Methods Microbiomes Microbiota Microbiota (Symbiotic organisms) Microorganisms Obesity Observations Open source software Parameter identification Patient outcomes Patients Statistical analysis Surgery Symbiosis Taxonomy Variance analysis Veganism |
| title | Roux-en-Y gastric bypass surgery changes fungal and bacterial microbiota in morbidly obese patients—A pilot study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-16T02%3A30%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Roux-en-Y%20gastric%20bypass%20surgery%20changes%20fungal%20and%20bacterial%20microbiota%20in%20morbidly%20obese%20patients%E2%80%94A%20pilot%20study&rft.jtitle=PloS%20one&rft.au=Steinert,%20Robert%20E&rft.date=2020-07-31&rft.volume=15&rft.issue=7&rft.spage=e0236936&rft.epage=e0236936&rft.pages=e0236936-e0236936&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0236936&rft_dat=%3Cgale_plos_%3EA631178112%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2429431719&rft_id=info:pmid/32735609&rft_galeid=A631178112&rft_doaj_id=oai_doaj_org_article_f4755c5106de4f3686aef8605eaf5368&rfr_iscdi=true |