The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella
The injection of laboratory animals with pathogenic microorganisms poses a significant safety risk because of the potential for injury by accidental needlestick. This is especially true for researchers using invertebrate models of disease due to the required precision and accuracy of the injection....
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| Veröffentlicht in: | PloS one 2020-07, Vol.15 (7), p.e0230767-e0230767 |
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| creator | Fredericks, Lance R Lee, Mark D Roslund, Cooper R Crabtree, Angela M Allen, Peter B Rowley, Paul A |
| description | The injection of laboratory animals with pathogenic microorganisms poses a significant safety risk because of the potential for injury by accidental needlestick. This is especially true for researchers using invertebrate models of disease due to the required precision and accuracy of the injection. The restraint of the greater wax moth larvae (Galleria mellonella) is often achieved by grasping a larva firmly between finger and thumb. Needle resistant gloves or forceps can be used to reduce the risk of a needlestick but can result in animal injury, a loss of throughput, and inconsistencies in experimental data. Restraint devices are commonly used for the manipulation of small mammals, and in this manuscript, we describe the construction of two devices that can be used to entrap and restrain G. mellonella larvae prior to injection with pathogenic microbes. These devices reduce the manual handling of larvae and provide an engineering control to protect against accidental needlestick injury while maintaining a high rate of injection. |
| doi_str_mv | 10.1371/journal.pone.0230767 |
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This is especially true for researchers using invertebrate models of disease due to the required precision and accuracy of the injection. The restraint of the greater wax moth larvae (Galleria mellonella) is often achieved by grasping a larva firmly between finger and thumb. Needle resistant gloves or forceps can be used to reduce the risk of a needlestick but can result in animal injury, a loss of throughput, and inconsistencies in experimental data. Restraint devices are commonly used for the manipulation of small mammals, and in this manuscript, we describe the construction of two devices that can be used to entrap and restrain G. mellonella larvae prior to injection with pathogenic microbes. These devices reduce the manual handling of larvae and provide an engineering control to protect against accidental needlestick injury while maintaining a high rate of injection.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0230767</identifier><identifier>PMID: 32730254</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Accident Prevention ; Animals ; Biology and Life Sciences ; Butterflies & moths ; Constraints ; Decontamination - instrumentation ; Electronic devices ; Engineering and Technology ; Equipment Reuse ; Galleria mellonella ; Gloves ; Injection ; Injections - instrumentation ; Injuries ; Invertebrates ; Laboratory animals ; Larva - microbiology ; Larvae ; Mammals ; Medical instruments ; Medicine and Health Sciences ; Methods ; Microbiology - instrumentation ; Microorganisms ; Moths - microbiology ; Motor ability ; Needlestick injuries ; Pathogens ; Physical Sciences ; Physiology ; Porous materials ; Risk reduction ; Safety engineering</subject><ispartof>PloS one, 2020-07, Vol.15 (7), p.e0230767-e0230767</ispartof><rights>2020 Fredericks et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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| subjects | Accident Prevention Animals Biology and Life Sciences Butterflies & moths Constraints Decontamination - instrumentation Electronic devices Engineering and Technology Equipment Reuse Galleria mellonella Gloves Injection Injections - instrumentation Injuries Invertebrates Laboratory animals Larva - microbiology Larvae Mammals Medical instruments Medicine and Health Sciences Methods Microbiology - instrumentation Microorganisms Moths - microbiology Motor ability Needlestick injuries Pathogens Physical Sciences Physiology Porous materials Risk reduction Safety engineering |
| title | The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella |
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