Dihydroxyacetone of wheat root exudates serves as an attractant for Heterodera avenae
Heterodera avenae, as an obligate endoparasite, causes severe yield loss in wheat (Triticum aestivum). Investigation on the mechanisms how H. avenae perceives wheat roots is limited. Here, the attractiveness of root exudates from eight plant genotypes to H. avenae were evaluated on agar plates. Resu...
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| creator | Wang, Gaofeng Wang, Yunhe Abdelnabby, Hazem Xiao, Xueqiong Huang, Wenkun Peng, Deliang Xiao, Yannong |
| description | Heterodera avenae, as an obligate endoparasite, causes severe yield loss in wheat (Triticum aestivum). Investigation on the mechanisms how H. avenae perceives wheat roots is limited. Here, the attractiveness of root exudates from eight plant genotypes to H. avenae were evaluated on agar plates. Results showed that the attraction of H. avenae to the root exudates from the non-host Brachypodium distachyon variety Bd21-3 was the highest, approximately 50 infective second-stage juveniles (J2s) per plate, followed by that from three H. avenae-susceptible wheat varieties, Zhengmai9023, Yanmai84 and Xiangmai25, as well as the resistant one of Xinyuan958, whereas the lowest attractive activity was observed in the two H. avenae-resistant wheat varieties, Xianmai20 (approximately 12 J2s/plate) and Liangxing66 (approximately 11 J2s/plate). Then Bd21-3, Zhengmai9023 and Heng4399 were selected for further assays as their different attractiveness and resistance to H. avenae, and attractants for H. avenae in their root exudates were characterized to be heat-labile and low-molecular compounds (LM) by behavioral bioassay. Based on these properties of the attractants, a principle of identifying attractants for H. avenae was set up. Then LM of six root exudates from the three plants with and without heating were separated and analyzed by HPLC-MS. Finally, dihydroxyacetone (DHA), methylprednisolone succinate, embelin and diethylpropionin in the root exudates were identified to be putative attractants for H. avenae according to the principle, and the attraction of DHA to H. avenae was validated by behavioral bioassay on agar. Our study enhances the recognition to the orientation mechanism of H. avenae towards wheat roots. |
| doi_str_mv | 10.1371/journal.pone.0236317 |
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Investigation on the mechanisms how H. avenae perceives wheat roots is limited. Here, the attractiveness of root exudates from eight plant genotypes to H. avenae were evaluated on agar plates. Results showed that the attraction of H. avenae to the root exudates from the non-host Brachypodium distachyon variety Bd21-3 was the highest, approximately 50 infective second-stage juveniles (J2s) per plate, followed by that from three H. avenae-susceptible wheat varieties, Zhengmai9023, Yanmai84 and Xiangmai25, as well as the resistant one of Xinyuan958, whereas the lowest attractive activity was observed in the two H. avenae-resistant wheat varieties, Xianmai20 (approximately 12 J2s/plate) and Liangxing66 (approximately 11 J2s/plate). Then Bd21-3, Zhengmai9023 and Heng4399 were selected for further assays as their different attractiveness and resistance to H. avenae, and attractants for H. avenae in their root exudates were characterized to be heat-labile and low-molecular compounds (LM) by behavioral bioassay. Based on these properties of the attractants, a principle of identifying attractants for H. avenae was set up. Then LM of six root exudates from the three plants with and without heating were separated and analyzed by HPLC-MS. Finally, dihydroxyacetone (DHA), methylprednisolone succinate, embelin and diethylpropionin in the root exudates were identified to be putative attractants for H. avenae according to the principle, and the attraction of DHA to H. avenae was validated by behavioral bioassay on agar. Our study enhances the recognition to the orientation mechanism of H. avenae towards wheat roots.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0236317</identifier><identifier>PMID: 32702002</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Attractants ; Attraction ; Bioassay ; Bioassays ; Biology and Life Sciences ; Brachypodium - genetics ; Brachypodium - parasitology ; Crop yield ; Dihydroxyacetone ; Dihydroxyacetone - chemistry ; Dihydroxyacetone - physiology ; Disease Resistance - genetics ; Disease Resistance - physiology ; Endoparasites ; Exudates ; Exudation ; Funding ; Genotype ; Genotypes ; Heterodera avenae ; High-performance liquid chromatography ; Laboratories ; Liquid chromatography ; Medicine and Health Sciences ; Methylprednisolone ; Nematodes ; Physical Sciences ; Plant diseases ; Plant Diseases - genetics ; Plant Diseases - parasitology ; Plant pathology ; Plant Roots - chemistry ; Plant Roots - genetics ; Plant Roots - parasitology ; Plant Roots - physiology ; Plant sciences ; Research and Analysis Methods ; Roots ; Triticum - chemistry ; Triticum - genetics ; Triticum - parasitology ; Triticum - physiology ; Triticum aestivum ; Tylenchoidea - genetics ; Tylenchoidea - pathogenicity ; Wheat</subject><ispartof>PloS one, 2020-07, Vol.15 (7), p.e0236317-e0236317</ispartof><rights>2020 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Wang et al 2020 Wang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-695a67117bb6ac97545fb95966bb923eb6dd81d2aec1bdfb7bbe236b7bf059fd3</citedby><cites>FETCH-LOGICAL-c526t-695a67117bb6ac97545fb95966bb923eb6dd81d2aec1bdfb7bbe236b7bf059fd3</cites><orcidid>0000-0003-2231-3254</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377440/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377440/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32702002$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Saia, Sergio</contributor><creatorcontrib>Wang, Gaofeng</creatorcontrib><creatorcontrib>Wang, Yunhe</creatorcontrib><creatorcontrib>Abdelnabby, Hazem</creatorcontrib><creatorcontrib>Xiao, Xueqiong</creatorcontrib><creatorcontrib>Huang, Wenkun</creatorcontrib><creatorcontrib>Peng, Deliang</creatorcontrib><creatorcontrib>Xiao, Yannong</creatorcontrib><title>Dihydroxyacetone of wheat root exudates serves as an attractant for Heterodera avenae</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Heterodera avenae, as an obligate endoparasite, causes severe yield loss in wheat (Triticum aestivum). Investigation on the mechanisms how H. avenae perceives wheat roots is limited. Here, the attractiveness of root exudates from eight plant genotypes to H. avenae were evaluated on agar plates. Results showed that the attraction of H. avenae to the root exudates from the non-host Brachypodium distachyon variety Bd21-3 was the highest, approximately 50 infective second-stage juveniles (J2s) per plate, followed by that from three H. avenae-susceptible wheat varieties, Zhengmai9023, Yanmai84 and Xiangmai25, as well as the resistant one of Xinyuan958, whereas the lowest attractive activity was observed in the two H. avenae-resistant wheat varieties, Xianmai20 (approximately 12 J2s/plate) and Liangxing66 (approximately 11 J2s/plate). Then Bd21-3, Zhengmai9023 and Heng4399 were selected for further assays as their different attractiveness and resistance to H. avenae, and attractants for H. avenae in their root exudates were characterized to be heat-labile and low-molecular compounds (LM) by behavioral bioassay. Based on these properties of the attractants, a principle of identifying attractants for H. avenae was set up. Then LM of six root exudates from the three plants with and without heating were separated and analyzed by HPLC-MS. Finally, dihydroxyacetone (DHA), methylprednisolone succinate, embelin and diethylpropionin in the root exudates were identified to be putative attractants for H. avenae according to the principle, and the attraction of DHA to H. avenae was validated by behavioral bioassay on agar. Our study enhances the recognition to the orientation mechanism of H. avenae towards wheat roots.</description><subject>Animals</subject><subject>Attractants</subject><subject>Attraction</subject><subject>Bioassay</subject><subject>Bioassays</subject><subject>Biology and Life Sciences</subject><subject>Brachypodium - genetics</subject><subject>Brachypodium - parasitology</subject><subject>Crop yield</subject><subject>Dihydroxyacetone</subject><subject>Dihydroxyacetone - chemistry</subject><subject>Dihydroxyacetone - physiology</subject><subject>Disease Resistance - genetics</subject><subject>Disease Resistance - physiology</subject><subject>Endoparasites</subject><subject>Exudates</subject><subject>Exudation</subject><subject>Funding</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Heterodera avenae</subject><subject>High-performance liquid chromatography</subject><subject>Laboratories</subject><subject>Liquid chromatography</subject><subject>Medicine and Health Sciences</subject><subject>Methylprednisolone</subject><subject>Nematodes</subject><subject>Physical Sciences</subject><subject>Plant diseases</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - parasitology</subject><subject>Plant pathology</subject><subject>Plant Roots - chemistry</subject><subject>Plant Roots - genetics</subject><subject>Plant Roots - parasitology</subject><subject>Plant Roots - physiology</subject><subject>Plant sciences</subject><subject>Research and Analysis Methods</subject><subject>Roots</subject><subject>Triticum - chemistry</subject><subject>Triticum - genetics</subject><subject>Triticum - parasitology</subject><subject>Triticum - physiology</subject><subject>Triticum aestivum</subject><subject>Tylenchoidea - genetics</subject><subject>Tylenchoidea - pathogenicity</subject><subject>Wheat</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUl1rFDEUHUSxtfoPRAd88WXXfEySyYsg9aOFgi_2Odwkd7qzzE7WJLN2_73Z7rS0IgRuSM45957Lqaq3lCwpV_TTOkxxhGG5DSMuCeOSU_WsOqWas4VkhD9_dD-pXqW0JkTwVsqX1QlnijBC2Gl1_bVf7X0Mt3twmItUHbr6zwoh1zGEXOPt5CFjqhPGXSlQzlhDzhFchjHXXYj1BWaMwWOEGnY4Ar6uXnQwJHwz17Pq-vu3X-cXi6ufPy7Pv1wtnGAyL6QWIBWlyloJTivRiM5qoaW0VjOOVnrfUs8AHbW-swWHxWipHRG68_ysen_U3Q4hmXkjybCGScGppLIgLo8IH2BttrHfQNybAL25ewjxxkDMvRvQMGIda1vbNtI3pBXAXdsBeE4bi6RRRevz3G2yG_QOx7KF4Yno05-xX5mbsDOKK9U0pAh8nAVi-D1hymbTJ4fDACOG6W5uxYmW-tDrwz_Q_7trjigXQ0oRu4dhKDGHlNyzzCElZk5Job17bOSBdB8L_heygrx_</recordid><startdate>20200723</startdate><enddate>20200723</enddate><creator>Wang, Gaofeng</creator><creator>Wang, Yunhe</creator><creator>Abdelnabby, Hazem</creator><creator>Xiao, Xueqiong</creator><creator>Huang, Wenkun</creator><creator>Peng, Deliang</creator><creator>Xiao, Yannong</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-2231-3254</orcidid></search><sort><creationdate>20200723</creationdate><title>Dihydroxyacetone of wheat root exudates serves as an attractant for Heterodera avenae</title><author>Wang, Gaofeng ; Wang, Yunhe ; Abdelnabby, Hazem ; Xiao, Xueqiong ; Huang, Wenkun ; Peng, Deliang ; Xiao, Yannong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-695a67117bb6ac97545fb95966bb923eb6dd81d2aec1bdfb7bbe236b7bf059fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Attractants</topic><topic>Attraction</topic><topic>Bioassay</topic><topic>Bioassays</topic><topic>Biology and Life Sciences</topic><topic>Brachypodium - genetics</topic><topic>Brachypodium - parasitology</topic><topic>Crop yield</topic><topic>Dihydroxyacetone</topic><topic>Dihydroxyacetone - chemistry</topic><topic>Dihydroxyacetone - physiology</topic><topic>Disease Resistance - genetics</topic><topic>Disease Resistance - physiology</topic><topic>Endoparasites</topic><topic>Exudates</topic><topic>Exudation</topic><topic>Funding</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Heterodera avenae</topic><topic>High-performance liquid chromatography</topic><topic>Laboratories</topic><topic>Liquid chromatography</topic><topic>Medicine and Health Sciences</topic><topic>Methylprednisolone</topic><topic>Nematodes</topic><topic>Physical Sciences</topic><topic>Plant diseases</topic><topic>Plant Diseases - genetics</topic><topic>Plant Diseases - parasitology</topic><topic>Plant pathology</topic><topic>Plant Roots - chemistry</topic><topic>Plant Roots - genetics</topic><topic>Plant Roots - parasitology</topic><topic>Plant Roots - physiology</topic><topic>Plant sciences</topic><topic>Research and Analysis Methods</topic><topic>Roots</topic><topic>Triticum - 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Investigation on the mechanisms how H. avenae perceives wheat roots is limited. Here, the attractiveness of root exudates from eight plant genotypes to H. avenae were evaluated on agar plates. Results showed that the attraction of H. avenae to the root exudates from the non-host Brachypodium distachyon variety Bd21-3 was the highest, approximately 50 infective second-stage juveniles (J2s) per plate, followed by that from three H. avenae-susceptible wheat varieties, Zhengmai9023, Yanmai84 and Xiangmai25, as well as the resistant one of Xinyuan958, whereas the lowest attractive activity was observed in the two H. avenae-resistant wheat varieties, Xianmai20 (approximately 12 J2s/plate) and Liangxing66 (approximately 11 J2s/plate). Then Bd21-3, Zhengmai9023 and Heng4399 were selected for further assays as their different attractiveness and resistance to H. avenae, and attractants for H. avenae in their root exudates were characterized to be heat-labile and low-molecular compounds (LM) by behavioral bioassay. Based on these properties of the attractants, a principle of identifying attractants for H. avenae was set up. Then LM of six root exudates from the three plants with and without heating were separated and analyzed by HPLC-MS. Finally, dihydroxyacetone (DHA), methylprednisolone succinate, embelin and diethylpropionin in the root exudates were identified to be putative attractants for H. avenae according to the principle, and the attraction of DHA to H. avenae was validated by behavioral bioassay on agar. Our study enhances the recognition to the orientation mechanism of H. avenae towards wheat roots.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32702002</pmid><doi>10.1371/journal.pone.0236317</doi><orcidid>https://orcid.org/0000-0003-2231-3254</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Attractants Attraction Bioassay Bioassays Biology and Life Sciences Brachypodium - genetics Brachypodium - parasitology Crop yield Dihydroxyacetone Dihydroxyacetone - chemistry Dihydroxyacetone - physiology Disease Resistance - genetics Disease Resistance - physiology Endoparasites Exudates Exudation Funding Genotype Genotypes Heterodera avenae High-performance liquid chromatography Laboratories Liquid chromatography Medicine and Health Sciences Methylprednisolone Nematodes Physical Sciences Plant diseases Plant Diseases - genetics Plant Diseases - parasitology Plant pathology Plant Roots - chemistry Plant Roots - genetics Plant Roots - parasitology Plant Roots - physiology Plant sciences Research and Analysis Methods Roots Triticum - chemistry Triticum - genetics Triticum - parasitology Triticum - physiology Triticum aestivum Tylenchoidea - genetics Tylenchoidea - pathogenicity Wheat |
| title | Dihydroxyacetone of wheat root exudates serves as an attractant for Heterodera avenae |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T10%3A51%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dihydroxyacetone%20of%20wheat%20root%20exudates%20serves%20as%20an%20attractant%20for%20Heterodera%20avenae&rft.jtitle=PloS%20one&rft.au=Wang,%20Gaofeng&rft.date=2020-07-23&rft.volume=15&rft.issue=7&rft.spage=e0236317&rft.epage=e0236317&rft.pages=e0236317-e0236317&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0236317&rft_dat=%3Cproquest_plos_%3E2426531616%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2426531616&rft_id=info:pmid/32702002&rft_doaj_id=oai_doaj_org_article_20bc288b846d4085a3c8faad314be047&rfr_iscdi=true |