Pax6 organizes the anterior eye segment by guiding two distinct neural crest waves
Cranial neural crest (NC) contributes to the developing vertebrate eye. By multidimensional, quantitative imaging, we traced the origin of the ocular NC cells to two distinct NC populations that differ in the maintenance of sox10 expression, Wnt signalling, origin, route, mode and destination of mig...
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| creator | Takamiya, Masanari Stegmaier, Johannes Kobitski, Andrei Yu Schott, Benjamin Weger, Benjamin D Margariti, Dimitra Cereceda Delgado, Angel R Gourain, Victor Scherr, Tim Yang, Lixin Sorge, Sebastian Otte, Jens C Hartmann, Volker van Wezel, Jos Stotzka, Rainer Reinhard, Thomas Schlunck, Gunther Dickmeis, Thomas Rastegar, Sepand Mikut, Ralf Nienhaus, Gerd Ulrich Strahle, Uwe |
| description | Cranial neural crest (NC) contributes to the developing vertebrate eye. By multidimensional, quantitative imaging, we traced the origin of the ocular NC cells to two distinct NC populations that differ in the maintenance of sox10 expression, Wnt signalling, origin, route, mode and destination of migration. The first NC population migrates to the proximal and the second NC cell group populates the distal (anterior) part of the eye. By analysing zebrafish pax6a/b compound mutants presenting anterior segment dysgenesis, we demonstrate that Pax6a/b guide the two NC populations to distinct proximodistal locations. We further provide evidence that the lens whose formation is pax6a/b-dependent and lens-derived TGF[beta] signals contribute to the building of the anterior segment. Taken together, our results reveal multiple roles of Pax6a/b in the control of NC cells during development of the anterior segment. |
| doi_str_mv | 10.1371/journal.pgen.1008774 |
| format | Article |
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By multidimensional, quantitative imaging, we traced the origin of the ocular NC cells to two distinct NC populations that differ in the maintenance of sox10 expression, Wnt signalling, origin, route, mode and destination of migration. The first NC population migrates to the proximal and the second NC cell group populates the distal (anterior) part of the eye. By analysing zebrafish pax6a/b compound mutants presenting anterior segment dysgenesis, we demonstrate that Pax6a/b guide the two NC populations to distinct proximodistal locations. We further provide evidence that the lens whose formation is pax6a/b-dependent and lens-derived TGF[beta] signals contribute to the building of the anterior segment. Taken together, our results reveal multiple roles of Pax6a/b in the control of NC cells during development of the anterior segment.</description><identifier>ISSN: 1553-7404</identifier><identifier>ISSN: 1553-7390</identifier><identifier>EISSN: 1553-7404</identifier><identifier>DOI: 10.1371/journal.pgen.1008774</identifier><identifier>PMID: 32555736</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Anterior segment dysgenesis syndrome ; Applied physics ; Automation ; Biology and Life Sciences ; Cell development (Biology) ; Cornea ; Cytological research ; Data processing ; Defects ; Endothelium ; Eye ; Gene expression ; Informatics ; Information processing ; Mammals ; Medicine and Health Sciences ; Neural crest ; Organogenesis ; Pax6 protein ; Physiological aspects ; Research and Analysis Methods ; Sox10 protein ; Stem cells ; Wnt protein</subject><ispartof>PLoS genetics, 2020-06, Vol.16 (6), p.e1008774-e1008774</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Takamiya et al. 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By multidimensional, quantitative imaging, we traced the origin of the ocular NC cells to two distinct NC populations that differ in the maintenance of sox10 expression, Wnt signalling, origin, route, mode and destination of migration. The first NC population migrates to the proximal and the second NC cell group populates the distal (anterior) part of the eye. By analysing zebrafish pax6a/b compound mutants presenting anterior segment dysgenesis, we demonstrate that Pax6a/b guide the two NC populations to distinct proximodistal locations. We further provide evidence that the lens whose formation is pax6a/b-dependent and lens-derived TGF[beta] signals contribute to the building of the anterior segment. 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| identifier | ISSN: 1553-7404 |
| ispartof | PLoS genetics, 2020-06, Vol.16 (6), p.e1008774-e1008774 |
| issn | 1553-7404 1553-7390 1553-7404 |
| language | eng |
| recordid | cdi_plos_journals_2424468859 |
| source | DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Anterior segment dysgenesis syndrome Applied physics Automation Biology and Life Sciences Cell development (Biology) Cornea Cytological research Data processing Defects Endothelium Eye Gene expression Informatics Information processing Mammals Medicine and Health Sciences Neural crest Organogenesis Pax6 protein Physiological aspects Research and Analysis Methods Sox10 protein Stem cells Wnt protein |
| title | Pax6 organizes the anterior eye segment by guiding two distinct neural crest waves |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T04%3A40%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pax6%20organizes%20the%20anterior%20eye%20segment%20by%20guiding%20two%20distinct%20neural%20crest%20waves&rft.jtitle=PLoS%20genetics&rft.au=Takamiya,%20Masanari&rft.date=2020-06-17&rft.volume=16&rft.issue=6&rft.spage=e1008774&rft.epage=e1008774&rft.pages=e1008774-e1008774&rft.issn=1553-7404&rft.eissn=1553-7404&rft_id=info:doi/10.1371/journal.pgen.1008774&rft_dat=%3Cgale_plos_%3EA632948907%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2424468859&rft_id=info:pmid/32555736&rft_galeid=A632948907&rft_doaj_id=oai_doaj_org_article_8e4da45e746442678c656ac1c39e9a74&rfr_iscdi=true |