Detection of arboviruses in mosquitoes: Evidence of circulation of chikungunya virus in Iran
Mosquitoes are vectors of viruses affecting animal and human health. In Iran, the prevalence of mosquito-borne viruses remains poorly investigated. Once infected, mosquito females remain infected for all their life making virus detections possible at early steps before infections are reported in ver...
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| Veröffentlicht in: | PLoS neglected tropical diseases 2020-06, Vol.14 (6), p.e0008135-e0008135 |
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| creator | Bakhshi, Hasan Mousson, Laurence Moutailler, Sara Vazeille, Marie Piorkowski, Géraldine Zakeri, Sedigheh Raz, Abbasali de Lamballerie, Xavier Dinparast-Djadid, Navid Failloux, Anna-Bella |
| description | Mosquitoes are vectors of viruses affecting animal and human health. In Iran, the prevalence of mosquito-borne viruses remains poorly investigated. Once infected, mosquito females remain infected for all their life making virus detections possible at early steps before infections are reported in vertebrate hosts. In this study, we used a recently developed high-throughput chip based on the BioMark Dynamic arrays system capable of detecting 37 arboviruses in a single experiment. A total of 1,212 mosquitoes collected in Mazandaran, North-Khorasan, and Fars provinces of Iran were analyzed. Eighteen species were identified, belonging to five genera; the most prevalent species were Anopheles maculipennis s.l. (42.41%), Culex pipiens (19.39%), An. superpictus (11.72%), and Cx. tritaeniorhynchus (10.64%). We detected chikungunya virus (CHIKV) of the Asian genotype in six mosquito pools collected in North Khorasan and Mazandaran provinces. To our knowledge, this is the first report of mosquitoes infected with CHIKV in Iran. Our high-throughput screening method can be proposed as a novel epidemiological surveillance tool to identify circulating arboviruses and to support preparedness to an epidemic in animals and humans. |
| doi_str_mv | 10.1371/journal.pntd.0008135 |
| format | Article |
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In Iran, the prevalence of mosquito-borne viruses remains poorly investigated. Once infected, mosquito females remain infected for all their life making virus detections possible at early steps before infections are reported in vertebrate hosts. In this study, we used a recently developed high-throughput chip based on the BioMark Dynamic arrays system capable of detecting 37 arboviruses in a single experiment. A total of 1,212 mosquitoes collected in Mazandaran, North-Khorasan, and Fars provinces of Iran were analyzed. Eighteen species were identified, belonging to five genera; the most prevalent species were Anopheles maculipennis s.l. (42.41%), Culex pipiens (19.39%), An. superpictus (11.72%), and Cx. tritaeniorhynchus (10.64%). We detected chikungunya virus (CHIKV) of the Asian genotype in six mosquito pools collected in North Khorasan and Mazandaran provinces. To our knowledge, this is the first report of mosquitoes infected with CHIKV in Iran. Our high-throughput screening method can be proposed as a novel epidemiological surveillance tool to identify circulating arboviruses and to support preparedness to an epidemic in animals and humans.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0008135</identifier><identifier>PMID: 32603322</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Anopheles maculipennis ; Anopheles superpictus ; Aquatic insects ; Arboviruses ; Biology and Life Sciences ; Biotechnology ; Chikungunya virus ; Chikungunya virus - isolation & purification ; Collections ; Culex pipiens ; Culex tritaeniorhynchus ; Culicidae ; Culicidae - classification ; Culicidae - virology ; Distribution ; Epidemics ; Epidemiology ; Female ; Funding ; Gene expression ; Genotypes ; High-throughput screening ; Identification and classification ; Infections ; Iran ; Life Sciences ; Malaria ; Male ; Mathematical analysis ; Medicine and Health Sciences ; Microbiology and Parasitology ; Mosquitoes ; People and Places ; Provinces ; Risk factors ; Thermal cycling ; Tropical diseases ; Vector-borne diseases ; Vectors ; Vertebrates ; Virology ; Viruses ; West Nile virus</subject><ispartof>PLoS neglected tropical diseases, 2020-06, Vol.14 (6), p.e0008135-e0008135</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Bakhshi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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In Iran, the prevalence of mosquito-borne viruses remains poorly investigated. Once infected, mosquito females remain infected for all their life making virus detections possible at early steps before infections are reported in vertebrate hosts. In this study, we used a recently developed high-throughput chip based on the BioMark Dynamic arrays system capable of detecting 37 arboviruses in a single experiment. A total of 1,212 mosquitoes collected in Mazandaran, North-Khorasan, and Fars provinces of Iran were analyzed. Eighteen species were identified, belonging to five genera; the most prevalent species were Anopheles maculipennis s.l. (42.41%), Culex pipiens (19.39%), An. superpictus (11.72%), and Cx. tritaeniorhynchus (10.64%). We detected chikungunya virus (CHIKV) of the Asian genotype in six mosquito pools collected in North Khorasan and Mazandaran provinces. To our knowledge, this is the first report of mosquitoes infected with CHIKV in Iran. Our high-throughput screening method can be proposed as a novel epidemiological surveillance tool to identify circulating arboviruses and to support preparedness to an epidemic in animals and humans.</description><subject>Animals</subject><subject>Anopheles maculipennis</subject><subject>Anopheles superpictus</subject><subject>Aquatic insects</subject><subject>Arboviruses</subject><subject>Biology and Life Sciences</subject><subject>Biotechnology</subject><subject>Chikungunya virus</subject><subject>Chikungunya virus - isolation & purification</subject><subject>Collections</subject><subject>Culex pipiens</subject><subject>Culex tritaeniorhynchus</subject><subject>Culicidae</subject><subject>Culicidae - classification</subject><subject>Culicidae - virology</subject><subject>Distribution</subject><subject>Epidemics</subject><subject>Epidemiology</subject><subject>Female</subject><subject>Funding</subject><subject>Gene 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of arboviruses in mosquitoes: Evidence of circulation of chikungunya virus in Iran</title><author>Bakhshi, Hasan ; Mousson, Laurence ; Moutailler, Sara ; Vazeille, Marie ; Piorkowski, Géraldine ; Zakeri, Sedigheh ; Raz, Abbasali ; de Lamballerie, Xavier ; Dinparast-Djadid, Navid ; Failloux, Anna-Bella</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c662t-519fc7196e54828c54daf7e3f07c5a449a0cb3d9caf9c79737ed5680dc61ae533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Anopheles maculipennis</topic><topic>Anopheles superpictus</topic><topic>Aquatic insects</topic><topic>Arboviruses</topic><topic>Biology and Life Sciences</topic><topic>Biotechnology</topic><topic>Chikungunya virus</topic><topic>Chikungunya virus - isolation & purification</topic><topic>Collections</topic><topic>Culex pipiens</topic><topic>Culex 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In Iran, the prevalence of mosquito-borne viruses remains poorly investigated. Once infected, mosquito females remain infected for all their life making virus detections possible at early steps before infections are reported in vertebrate hosts. In this study, we used a recently developed high-throughput chip based on the BioMark Dynamic arrays system capable of detecting 37 arboviruses in a single experiment. A total of 1,212 mosquitoes collected in Mazandaran, North-Khorasan, and Fars provinces of Iran were analyzed. Eighteen species were identified, belonging to five genera; the most prevalent species were Anopheles maculipennis s.l. (42.41%), Culex pipiens (19.39%), An. superpictus (11.72%), and Cx. tritaeniorhynchus (10.64%). We detected chikungunya virus (CHIKV) of the Asian genotype in six mosquito pools collected in North Khorasan and Mazandaran provinces. To our knowledge, this is the first report of mosquitoes infected with CHIKV in Iran. Our high-throughput screening method can be proposed as a novel epidemiological surveillance tool to identify circulating arboviruses and to support preparedness to an epidemic in animals and humans.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32603322</pmid><doi>10.1371/journal.pntd.0008135</doi><orcidid>https://orcid.org/0000-0001-6890-0820</orcidid><orcidid>https://orcid.org/0000-0003-3010-6968</orcidid><orcidid>https://orcid.org/0000-0003-4935-4662</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Public Library of Science (PLoS); PubMed Central |
| subjects | Animals Anopheles maculipennis Anopheles superpictus Aquatic insects Arboviruses Biology and Life Sciences Biotechnology Chikungunya virus Chikungunya virus - isolation & purification Collections Culex pipiens Culex tritaeniorhynchus Culicidae Culicidae - classification Culicidae - virology Distribution Epidemics Epidemiology Female Funding Gene expression Genotypes High-throughput screening Identification and classification Infections Iran Life Sciences Malaria Male Mathematical analysis Medicine and Health Sciences Microbiology and Parasitology Mosquitoes People and Places Provinces Risk factors Thermal cycling Tropical diseases Vector-borne diseases Vectors Vertebrates Virology Viruses West Nile virus |
| title | Detection of arboviruses in mosquitoes: Evidence of circulation of chikungunya virus in Iran |
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