Sensitivity of yeast to lithium chloride connects the activity of YTA6 and YPR096C to translation of structured mRNAs
Lithium Chloride (LiCl) toxicity, mode of action and cellular responses have been the subject of active investigations over the past decades. In yeast, LiCl treatment is reported to reduce the activity and alters the expression of PGM2, a gene that encodes a phosphoglucomutase involved in sugar meta...
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| creator | Hajikarimlou, Maryam Moteshareie, Houman Omidi, Katayoun Hooshyar, Mohsen Shaikho, Sarah Kazmirchuk, Tom Burnside, Daniel Takallou, Sarah Zare, Narges Jagadeesan, Sasi Kumar Puchacz, Nathalie Babu, Mohan Smith, Myron Holcik, Martin Samanfar, Bahram Golshani, Ashkan Lustig, Arthur J |
| description | Lithium Chloride (LiCl) toxicity, mode of action and cellular responses have been the subject of active investigations over the past decades. In yeast, LiCl treatment is reported to reduce the activity and alters the expression of PGM2, a gene that encodes a phosphoglucomutase involved in sugar metabolism. Reduced activity of phosphoglucomutase in the presence of galactose causes an accumulation of intermediate metabolites of galactose metabolism leading to a number of phenotypes including growth defect. In the current study, we identify two understudied yeast genes, YTA6 and YPR096C that when deleted, cell sensitivity to LiCl is increased when galactose is used as a carbon source. The 5'-UTR of PGM2 mRNA is structured. Using this region, we show that YTA6 and YPR096C influence the translation of PGM2 mRNA. |
| doi_str_mv | 10.1371/journal.pone.0235033 |
| format | Article |
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In yeast, LiCl treatment is reported to reduce the activity and alters the expression of PGM2, a gene that encodes a phosphoglucomutase involved in sugar metabolism. Reduced activity of phosphoglucomutase in the presence of galactose causes an accumulation of intermediate metabolites of galactose metabolism leading to a number of phenotypes including growth defect. In the current study, we identify two understudied yeast genes, YTA6 and YPR096C that when deleted, cell sensitivity to LiCl is increased when galactose is used as a carbon source. The 5'-UTR of PGM2 mRNA is structured. Using this region, we show that YTA6 and YPR096C influence the translation of PGM2 mRNA.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0235033</identifier><identifier>PMID: 32639961</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Amino acids ; Biology ; Biology and Life Sciences ; Carbon sources ; Cellular structure ; E coli ; Galactose ; Galactose metabolism ; Gene expression ; Genes ; Genetic aspects ; Glucose ; Growth disorders ; Health aspects ; Kinases ; Lithium ; Lithium chloride ; Lithium chlorides ; Lithium compounds ; Metabolism ; Metabolites ; Mode of action ; mRNA ; Phenotypes ; Phosphoglucomutase ; Physical Sciences ; Physiological aspects ; Plasmids ; Proteins ; R&D ; Research & development ; Research and Analysis Methods ; Risk factors ; Sensitivity ; Sensitivity analysis ; Signal transduction ; Toxicity ; Translation ; Y chromosomes ; Yeast ; Yeasts ; Yeasts (Fungi)</subject><ispartof>PloS one, 2020-07, Vol.15 (7), p.e0235033-e0235033</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Hajikarimlou et al. 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In yeast, LiCl treatment is reported to reduce the activity and alters the expression of PGM2, a gene that encodes a phosphoglucomutase involved in sugar metabolism. Reduced activity of phosphoglucomutase in the presence of galactose causes an accumulation of intermediate metabolites of galactose metabolism leading to a number of phenotypes including growth defect. In the current study, we identify two understudied yeast genes, YTA6 and YPR096C that when deleted, cell sensitivity to LiCl is increased when galactose is used as a carbon source. The 5'-UTR of PGM2 mRNA is structured. Using this region, we show that YTA6 and YPR096C influence the translation of PGM2 mRNA.</description><subject>Amino acids</subject><subject>Biology</subject><subject>Biology and Life Sciences</subject><subject>Carbon sources</subject><subject>Cellular structure</subject><subject>E coli</subject><subject>Galactose</subject><subject>Galactose metabolism</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Glucose</subject><subject>Growth disorders</subject><subject>Health aspects</subject><subject>Kinases</subject><subject>Lithium</subject><subject>Lithium chloride</subject><subject>Lithium chlorides</subject><subject>Lithium compounds</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Mode of action</subject><subject>mRNA</subject><subject>Phenotypes</subject><subject>Phosphoglucomutase</subject><subject>Physical Sciences</subject><subject>Physiological 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of yeast to lithium chloride connects the activity of YTA6 and YPR096C to translation of structured mRNAs</title><author>Hajikarimlou, Maryam ; Moteshareie, Houman ; Omidi, Katayoun ; Hooshyar, Mohsen ; Shaikho, Sarah ; Kazmirchuk, Tom ; Burnside, Daniel ; Takallou, Sarah ; Zare, Narges ; Jagadeesan, Sasi Kumar ; Puchacz, Nathalie ; Babu, Mohan ; Smith, Myron ; Holcik, Martin ; Samanfar, Bahram ; Golshani, Ashkan ; Lustig, Arthur J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c669t-5413deda55a38bd4c4f563ee4346d63323fb774cbe23ed523d3b122971cd15903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Amino acids</topic><topic>Biology</topic><topic>Biology and Life Sciences</topic><topic>Carbon sources</topic><topic>Cellular structure</topic><topic>E coli</topic><topic>Galactose</topic><topic>Galactose metabolism</topic><topic>Gene 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In yeast, LiCl treatment is reported to reduce the activity and alters the expression of PGM2, a gene that encodes a phosphoglucomutase involved in sugar metabolism. Reduced activity of phosphoglucomutase in the presence of galactose causes an accumulation of intermediate metabolites of galactose metabolism leading to a number of phenotypes including growth defect. In the current study, we identify two understudied yeast genes, YTA6 and YPR096C that when deleted, cell sensitivity to LiCl is increased when galactose is used as a carbon source. The 5'-UTR of PGM2 mRNA is structured. Using this region, we show that YTA6 and YPR096C influence the translation of PGM2 mRNA.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>32639961</pmid><doi>10.1371/journal.pone.0235033</doi><tpages>e0235033</tpages><orcidid>https://orcid.org/0000-0003-2962-6819</orcidid><orcidid>https://orcid.org/0000-0003-2420-7164</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2020-07, Vol.15 (7), p.e0235033-e0235033 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2421428554 |
| source | DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Amino acids Biology Biology and Life Sciences Carbon sources Cellular structure E coli Galactose Galactose metabolism Gene expression Genes Genetic aspects Glucose Growth disorders Health aspects Kinases Lithium Lithium chloride Lithium chlorides Lithium compounds Metabolism Metabolites Mode of action mRNA Phenotypes Phosphoglucomutase Physical Sciences Physiological aspects Plasmids Proteins R&D Research & development Research and Analysis Methods Risk factors Sensitivity Sensitivity analysis Signal transduction Toxicity Translation Y chromosomes Yeast Yeasts Yeasts (Fungi) |
| title | Sensitivity of yeast to lithium chloride connects the activity of YTA6 and YPR096C to translation of structured mRNAs |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T13%3A36%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sensitivity%20of%20yeast%20to%20lithium%20chloride%20connects%20the%20activity%20of%20YTA6%20and%20YPR096C%20to%20translation%20of%20structured%20mRNAs&rft.jtitle=PloS%20one&rft.au=Hajikarimlou,%20Maryam&rft.date=2020-07-08&rft.volume=15&rft.issue=7&rft.spage=e0235033&rft.epage=e0235033&rft.pages=e0235033-e0235033&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0235033&rft_dat=%3Cgale_plos_%3EA628903429%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2421428554&rft_id=info:pmid/32639961&rft_galeid=A628903429&rft_doaj_id=oai_doaj_org_article_53e008f2d41e42c6b8717a121939d6e9&rfr_iscdi=true |