Diversity of phlebotomine sand flies and molecular detection of trypanosomatids in Brumadinho, Minas Gerais, Brazil
This study aimed to describe the sand fly fauna and detect trypanosomatids in these insects from Casa Branca, state of Minas Gerais, Brazil, an endemic area of both visceral (VL) and tegumentary leishmaniasis (TL). Sand flies were collected bimonthly from May 2013 to July 2014, using automatic light...
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| creator | Tanure, Aline Rêgo, Felipe Dutra Tonelli, Gabriel Barbosa Campos, Aldenise Martins Shimabukuro, Paloma Helena Fernandes Gontijo, Célia Maria Ferreira Paz, Gustavo Fontes Andrade-Filho, José Dilermando |
| description | This study aimed to describe the sand fly fauna and detect trypanosomatids in these insects from Casa Branca, state of Minas Gerais, Brazil, an endemic area of both visceral (VL) and tegumentary leishmaniasis (TL). Sand flies were collected bimonthly from May 2013 to July 2014, using automatic light traps exposed for three consecutive nights in peridomiciliary areas of nine houses with previous reports of VL and TL. ITS1-PCR and DNA sequencing were performed for trypanosomatids identification. A total of 16,771 sand flies were collected belonging to 23 species. The most abundant species was Nyssomyia whitmani (Antunes & Coutinho, 1939) (70.9%), followed by Lutzomyia longipalpis (Lutz & Neiva, 1912) (15.2%) and Migonemyia migonei (França, 1920) (9.1%). Leishmania amazonensis DNA was detected in Ny. whitmani (four pools) and Le. braziliensis DNA was detected in Psychodopygus lloydi (one pool). In seven pools of Ny. whitmani and in one pool of Lu. longipalpis positive for Leishmania DNA, the parasite species was not determined due to the low quality of the sequences. Moreover, DNA of Herpetomonas spp. was detected in Ny. whitmani (two pools) and Cortelezzii complex (one pool). DNA of Crithidia spp. was detected in Ny. whitmani and Ps. lloydi (both one pool). Our results suggest that Ny. whitmani may be involved in the transmission of Le. amazonensis in the study area. The molecular detection of Le. amazonensis suggests the presence of this species in a sylvatic cycle between vertebrate and invertebrate hosts in the region of Casa Branca. Our data also reveal the occurrence of other non-Leishmania trypanosomatids in sand flies in Casa Branca District. |
| doi_str_mv | 10.1371/journal.pone.0234445 |
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Sand flies were collected bimonthly from May 2013 to July 2014, using automatic light traps exposed for three consecutive nights in peridomiciliary areas of nine houses with previous reports of VL and TL. ITS1-PCR and DNA sequencing were performed for trypanosomatids identification. A total of 16,771 sand flies were collected belonging to 23 species. The most abundant species was Nyssomyia whitmani (Antunes & Coutinho, 1939) (70.9%), followed by Lutzomyia longipalpis (Lutz & Neiva, 1912) (15.2%) and Migonemyia migonei (França, 1920) (9.1%). Leishmania amazonensis DNA was detected in Ny. whitmani (four pools) and Le. braziliensis DNA was detected in Psychodopygus lloydi (one pool). In seven pools of Ny. whitmani and in one pool of Lu. longipalpis positive for Leishmania DNA, the parasite species was not determined due to the low quality of the sequences. Moreover, DNA of Herpetomonas spp. was detected in Ny. whitmani (two pools) and Cortelezzii complex (one pool). DNA of Crithidia spp. was detected in Ny. whitmani and Ps. lloydi (both one pool). Our results suggest that Ny. whitmani may be involved in the transmission of Le. amazonensis in the study area. The molecular detection of Le. amazonensis suggests the presence of this species in a sylvatic cycle between vertebrate and invertebrate hosts in the region of Casa Branca. Our data also reveal the occurrence of other non-Leishmania trypanosomatids in sand flies in Casa Branca District.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0234445</identifier><identifier>PMID: 32579586</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Biology and Life Sciences ; Deoxyribonucleic acid ; Diptera ; Distribution ; DNA ; DNA sequencing ; Ecology and Environmental Sciences ; Females ; Flies ; Gene sequencing ; Genetic aspects ; Identification ; Identification and classification ; Infections ; Insects ; Invertebrates ; Laboratories ; Leishmania ; Leishmaniasis ; Light traps ; Medicine and Health Sciences ; Natural history ; Nucleotide sequence ; Parasites ; Parasitic diseases ; People and places ; Pools ; Residential areas ; Risk factors ; Species ; Studies ; Tegumentary leishmaniasis ; Trypanosomatina ; Vector-borne diseases ; Vertebrates</subject><ispartof>PloS one, 2020-06, Vol.15 (6), p.e0234445-e0234445</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Tanure et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Tanure et al 2020 Tanure et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c669t-4a06a99da1d679eb7d601fe864300f7096c24751edff0674e9e20e7ccfd9d85a3</citedby><cites>FETCH-LOGICAL-c669t-4a06a99da1d679eb7d601fe864300f7096c24751edff0674e9e20e7ccfd9d85a3</cites><orcidid>0000-0002-5114-953X ; 0000-0002-9754-8464</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314019/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314019/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53769,53771,79346,79347</link.rule.ids></links><search><contributor>Yurchenko, Vyacheslav</contributor><creatorcontrib>Tanure, Aline</creatorcontrib><creatorcontrib>Rêgo, Felipe Dutra</creatorcontrib><creatorcontrib>Tonelli, Gabriel Barbosa</creatorcontrib><creatorcontrib>Campos, Aldenise Martins</creatorcontrib><creatorcontrib>Shimabukuro, Paloma Helena Fernandes</creatorcontrib><creatorcontrib>Gontijo, Célia Maria Ferreira</creatorcontrib><creatorcontrib>Paz, Gustavo Fontes</creatorcontrib><creatorcontrib>Andrade-Filho, José Dilermando</creatorcontrib><title>Diversity of phlebotomine sand flies and molecular detection of trypanosomatids in Brumadinho, Minas Gerais, Brazil</title><title>PloS one</title><description>This study aimed to describe the sand fly fauna and detect trypanosomatids in these insects from Casa Branca, state of Minas Gerais, Brazil, an endemic area of both visceral (VL) and tegumentary leishmaniasis (TL). 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DNA of Crithidia spp. was detected in Ny. whitmani and Ps. lloydi (both one pool). Our results suggest that Ny. whitmani may be involved in the transmission of Le. amazonensis in the study area. The molecular detection of Le. amazonensis suggests the presence of this species in a sylvatic cycle between vertebrate and invertebrate hosts in the region of Casa Branca. Our data also reveal the occurrence of other non-Leishmania trypanosomatids in sand flies in Casa Branca District.</description><subject>Biology and Life Sciences</subject><subject>Deoxyribonucleic acid</subject><subject>Diptera</subject><subject>Distribution</subject><subject>DNA</subject><subject>DNA sequencing</subject><subject>Ecology and Environmental Sciences</subject><subject>Females</subject><subject>Flies</subject><subject>Gene sequencing</subject><subject>Genetic aspects</subject><subject>Identification</subject><subject>Identification and classification</subject><subject>Infections</subject><subject>Insects</subject><subject>Invertebrates</subject><subject>Laboratories</subject><subject>Leishmania</subject><subject>Leishmaniasis</subject><subject>Light traps</subject><subject>Medicine and Health Sciences</subject><subject>Natural history</subject><subject>Nucleotide sequence</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>People and 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Fontes</au><au>Andrade-Filho, José Dilermando</au><au>Yurchenko, Vyacheslav</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diversity of phlebotomine sand flies and molecular detection of trypanosomatids in Brumadinho, Minas Gerais, Brazil</atitle><jtitle>PloS one</jtitle><date>2020-06-24</date><risdate>2020</risdate><volume>15</volume><issue>6</issue><spage>e0234445</spage><epage>e0234445</epage><pages>e0234445-e0234445</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>This study aimed to describe the sand fly fauna and detect trypanosomatids in these insects from Casa Branca, state of Minas Gerais, Brazil, an endemic area of both visceral (VL) and tegumentary leishmaniasis (TL). Sand flies were collected bimonthly from May 2013 to July 2014, using automatic light traps exposed for three consecutive nights in peridomiciliary areas of nine houses with previous reports of VL and TL. ITS1-PCR and DNA sequencing were performed for trypanosomatids identification. A total of 16,771 sand flies were collected belonging to 23 species. The most abundant species was Nyssomyia whitmani (Antunes & Coutinho, 1939) (70.9%), followed by Lutzomyia longipalpis (Lutz & Neiva, 1912) (15.2%) and Migonemyia migonei (França, 1920) (9.1%). Leishmania amazonensis DNA was detected in Ny. whitmani (four pools) and Le. braziliensis DNA was detected in Psychodopygus lloydi (one pool). In seven pools of Ny. whitmani and in one pool of Lu. longipalpis positive for Leishmania DNA, the parasite species was not determined due to the low quality of the sequences. Moreover, DNA of Herpetomonas spp. was detected in Ny. whitmani (two pools) and Cortelezzii complex (one pool). DNA of Crithidia spp. was detected in Ny. whitmani and Ps. lloydi (both one pool). Our results suggest that Ny. whitmani may be involved in the transmission of Le. amazonensis in the study area. The molecular detection of Le. amazonensis suggests the presence of this species in a sylvatic cycle between vertebrate and invertebrate hosts in the region of Casa Branca. Our data also reveal the occurrence of other non-Leishmania trypanosomatids in sand flies in Casa Branca District.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>32579586</pmid><doi>10.1371/journal.pone.0234445</doi><tpages>e0234445</tpages><orcidid>https://orcid.org/0000-0002-5114-953X</orcidid><orcidid>https://orcid.org/0000-0002-9754-8464</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Biology and Life Sciences Deoxyribonucleic acid Diptera Distribution DNA DNA sequencing Ecology and Environmental Sciences Females Flies Gene sequencing Genetic aspects Identification Identification and classification Infections Insects Invertebrates Laboratories Leishmania Leishmaniasis Light traps Medicine and Health Sciences Natural history Nucleotide sequence Parasites Parasitic diseases People and places Pools Residential areas Risk factors Species Studies Tegumentary leishmaniasis Trypanosomatina Vector-borne diseases Vertebrates |
| title | Diversity of phlebotomine sand flies and molecular detection of trypanosomatids in Brumadinho, Minas Gerais, Brazil |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T01%3A45%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Diversity%20of%20phlebotomine%20sand%20flies%20and%20molecular%20detection%20of%20trypanosomatids%20in%20Brumadinho,%20Minas%20Gerais,%20Brazil&rft.jtitle=PloS%20one&rft.au=Tanure,%20Aline&rft.date=2020-06-24&rft.volume=15&rft.issue=6&rft.spage=e0234445&rft.epage=e0234445&rft.pages=e0234445-e0234445&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0234445&rft_dat=%3Cgale_plos_%3EA627563358%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2416996795&rft_id=info:pmid/32579586&rft_galeid=A627563358&rft_doaj_id=oai_doaj_org_article_9aa8b433a2bc460a923ae3f099f3b65d&rfr_iscdi=true |