Species diversity revealed in Sigmella Hebard, 1929 (Blattodea, ectobiidae) based on morphology and four molecular species delimitation methods
Cockroaches are one of the major decomposers involved in biogeochemical cycles. Cockroaches have an amazing amount of diversity, but most of them remain unknown due to the shortage of the trained taxonomists and the limitations of morphology-based identification. We obtained 49 COI sequences (includ...
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| Veröffentlicht in: | PloS one 2020-06, Vol.15 (6), p.e0232821-e0232821 |
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| creator | Li, Meng Zhao, Qiongyao Chen, Rong He, Jiajun Peng, Tao Deng, Wenbo Che, Yanli Wang, Zongqing |
| description | Cockroaches are one of the major decomposers involved in biogeochemical cycles. Cockroaches have an amazing amount of diversity, but most of them remain unknown due to the shortage of the trained taxonomists and the limitations of morphology-based identification. We obtained 49 COI sequences (including 42 novel sequences) and 32 novel 28S sequences for 5 Sigmella morphospecies collected from 11 localities. Three are new to science: Sigmella digitalis sp. nov., Sigmella exserta sp. nov. and Sigmella normalis sp. nov. Based on four species delimitation methods (ABGD, GMYC, BINs and bPTP), a total of 6 molecular operational taxonomic units (MOTUs) were recovered for 5 morphospecies. These were then confirmed by tree building methods using COI and combined data (COI and 28S). We detected more than one MOTU in the morphospecies S. digitalis sp. nov., which can indicate genetic diversity. Detailed morphological evidence for each MOTU is provided to confirm these slight variations and we conclude that natural barriers are likely the main cause of genetic diversity. |
| doi_str_mv | 10.1371/journal.pone.0232821 |
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Cockroaches have an amazing amount of diversity, but most of them remain unknown due to the shortage of the trained taxonomists and the limitations of morphology-based identification. We obtained 49 COI sequences (including 42 novel sequences) and 32 novel 28S sequences for 5 Sigmella morphospecies collected from 11 localities. Three are new to science: Sigmella digitalis sp. nov., Sigmella exserta sp. nov. and Sigmella normalis sp. nov. Based on four species delimitation methods (ABGD, GMYC, BINs and bPTP), a total of 6 molecular operational taxonomic units (MOTUs) were recovered for 5 morphospecies. These were then confirmed by tree building methods using COI and combined data (COI and 28S). We detected more than one MOTU in the morphospecies S. digitalis sp. nov., which can indicate genetic diversity. Detailed morphological evidence for each MOTU is provided to confirm these slight variations and we conclude that natural barriers are likely the main cause of genetic diversity.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0232821</identifier><identifier>PMID: 32520927</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Biodiversity ; Biogeochemical cycles ; Biological research ; Biology and Life Sciences ; Blattoidea ; Cockroaches ; Computer and Information Sciences ; Datasets ; Deoxyribonucleic acid ; Digitalis ; DNA ; Entomology ; Genetic aspects ; Genetic diversity ; Identification and classification ; Medicine and Health Sciences ; Morphology ; New species ; Physiological aspects ; Research and Analysis Methods ; Species diversity</subject><ispartof>PloS one, 2020-06, Vol.15 (6), p.e0232821-e0232821</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Li et al 2020 Li et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c570t-7d4eb5ff655bdc20a779f99d149d3174e382fd24d98308c519ae5841fcd1ebc43</citedby><cites>FETCH-LOGICAL-c570t-7d4eb5ff655bdc20a779f99d149d3174e382fd24d98308c519ae5841fcd1ebc43</cites><orcidid>0000-0002-6295-7372</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286484/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286484/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23865,27923,27924,53790,53792,79371,79372</link.rule.ids></links><search><contributor>Zhang, Feng</contributor><creatorcontrib>Li, Meng</creatorcontrib><creatorcontrib>Zhao, Qiongyao</creatorcontrib><creatorcontrib>Chen, Rong</creatorcontrib><creatorcontrib>He, Jiajun</creatorcontrib><creatorcontrib>Peng, Tao</creatorcontrib><creatorcontrib>Deng, Wenbo</creatorcontrib><creatorcontrib>Che, Yanli</creatorcontrib><creatorcontrib>Wang, Zongqing</creatorcontrib><title>Species diversity revealed in Sigmella Hebard, 1929 (Blattodea, ectobiidae) based on morphology and four molecular species delimitation methods</title><title>PloS one</title><description>Cockroaches are one of the major decomposers involved in biogeochemical cycles. Cockroaches have an amazing amount of diversity, but most of them remain unknown due to the shortage of the trained taxonomists and the limitations of morphology-based identification. We obtained 49 COI sequences (including 42 novel sequences) and 32 novel 28S sequences for 5 Sigmella morphospecies collected from 11 localities. Three are new to science: Sigmella digitalis sp. nov., Sigmella exserta sp. nov. and Sigmella normalis sp. nov. Based on four species delimitation methods (ABGD, GMYC, BINs and bPTP), a total of 6 molecular operational taxonomic units (MOTUs) were recovered for 5 morphospecies. These were then confirmed by tree building methods using COI and combined data (COI and 28S). We detected more than one MOTU in the morphospecies S. digitalis sp. nov., which can indicate genetic diversity. Detailed morphological evidence for each MOTU is provided to confirm these slight variations and we conclude that natural barriers are likely the main cause of genetic diversity.</description><subject>Biodiversity</subject><subject>Biogeochemical cycles</subject><subject>Biological research</subject><subject>Biology and Life Sciences</subject><subject>Blattoidea</subject><subject>Cockroaches</subject><subject>Computer and Information Sciences</subject><subject>Datasets</subject><subject>Deoxyribonucleic acid</subject><subject>Digitalis</subject><subject>DNA</subject><subject>Entomology</subject><subject>Genetic aspects</subject><subject>Genetic diversity</subject><subject>Identification and classification</subject><subject>Medicine and Health Sciences</subject><subject>Morphology</subject><subject>New species</subject><subject>Physiological aspects</subject><subject>Research and Analysis Methods</subject><subject>Species 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diversity revealed in Sigmella Hebard, 1929 (Blattodea, ectobiidae) based on morphology and four molecular species delimitation methods</title><author>Li, Meng ; Zhao, Qiongyao ; Chen, Rong ; He, Jiajun ; Peng, Tao ; Deng, Wenbo ; Che, Yanli ; Wang, Zongqing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c570t-7d4eb5ff655bdc20a779f99d149d3174e382fd24d98308c519ae5841fcd1ebc43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biodiversity</topic><topic>Biogeochemical cycles</topic><topic>Biological research</topic><topic>Biology and Life Sciences</topic><topic>Blattoidea</topic><topic>Cockroaches</topic><topic>Computer and Information Sciences</topic><topic>Datasets</topic><topic>Deoxyribonucleic acid</topic><topic>Digitalis</topic><topic>DNA</topic><topic>Entomology</topic><topic>Genetic aspects</topic><topic>Genetic diversity</topic><topic>Identification and 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Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Meng</au><au>Zhao, Qiongyao</au><au>Chen, Rong</au><au>He, Jiajun</au><au>Peng, Tao</au><au>Deng, Wenbo</au><au>Che, Yanli</au><au>Wang, Zongqing</au><au>Zhang, Feng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Species diversity revealed in Sigmella Hebard, 1929 (Blattodea, ectobiidae) based on morphology and four molecular species delimitation methods</atitle><jtitle>PloS one</jtitle><date>2020-06-10</date><risdate>2020</risdate><volume>15</volume><issue>6</issue><spage>e0232821</spage><epage>e0232821</epage><pages>e0232821-e0232821</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Cockroaches are one of the major decomposers involved in biogeochemical cycles. Cockroaches have an amazing amount of diversity, but most of them remain unknown due to the shortage of the trained taxonomists and the limitations of morphology-based identification. We obtained 49 COI sequences (including 42 novel sequences) and 32 novel 28S sequences for 5 Sigmella morphospecies collected from 11 localities. Three are new to science: Sigmella digitalis sp. nov., Sigmella exserta sp. nov. and Sigmella normalis sp. nov. Based on four species delimitation methods (ABGD, GMYC, BINs and bPTP), a total of 6 molecular operational taxonomic units (MOTUs) were recovered for 5 morphospecies. These were then confirmed by tree building methods using COI and combined data (COI and 28S). We detected more than one MOTU in the morphospecies S. digitalis sp. nov., which can indicate genetic diversity. Detailed morphological evidence for each MOTU is provided to confirm these slight variations and we conclude that natural barriers are likely the main cause of genetic diversity.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>32520927</pmid><doi>10.1371/journal.pone.0232821</doi><orcidid>https://orcid.org/0000-0002-6295-7372</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Biodiversity Biogeochemical cycles Biological research Biology and Life Sciences Blattoidea Cockroaches Computer and Information Sciences Datasets Deoxyribonucleic acid Digitalis DNA Entomology Genetic aspects Genetic diversity Identification and classification Medicine and Health Sciences Morphology New species Physiological aspects Research and Analysis Methods Species diversity |
| title | Species diversity revealed in Sigmella Hebard, 1929 (Blattodea, ectobiidae) based on morphology and four molecular species delimitation methods |
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