Marker assisted selection of new high oleic and low linolenic winter oilseed rape (Brassica napus L.) inbred lines revealing good agricultural value
Development of oilseed rape (Brassica napus L.) breeding lines producing oil characterized by high oleic and low linolenic acid content is an important goal of rapeseed breeding programs worldwide. Such kind of oil is ideal for deep frying and can also be used as a raw material for biodiesel product...
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| creator | Spasibionek, Stanislaw Mikolajczyk, Katarzyna Cwiek-Kupczynska, Hanna Pietka, Teresa Krotka, Krystyna Matuszczak, Marcin Nowakowska, Joanna Michalski, Krzysztof Bartkowiak-Broda, Iwona |
| description | Development of oilseed rape (Brassica napus L.) breeding lines producing oil characterized by high oleic and low linolenic acid content is an important goal of rapeseed breeding programs worldwide. Such kind of oil is ideal for deep frying and can also be used as a raw material for biodiesel production. By performing chemical mutagenesis using ethyl methanesulfonate, we obtained mutant winter rapeseed breeding lines that can produce oil with a high content of oleic acid (C18:1, more than 75%) and a low content of linolenic acid (C18:3, less than 3%). However, the mutant lines revealed low agricultural value as they were characterized by low seed yield, low wintering, and high content of glucosinolates in seed meal. The aim of this work was to improve the mutant lines and develop high-oleic and low-linolenic recombinants exhibiting both good oil quality and high agronomic value. The plant materials used in this study included high-oleic and low-linolenic mutant breeding lines and high-yielding domestic canola-type breeding lines of good agricultural value with high oleic acid content and extremely low glucosinolates content. Field trials were conducted in four environments, in a randomized complete block design. Phenotyping was performed for wintering, yield of seed and oil, and seed quality traits. Genotype x environment interaction was investigated with respect to the content of C18:1 and C18:3 acids in seed oil. Genotyping was done for the selection of homozygous high oleic and low linolenic lines using allele-specific CAPS markers and SNaPshot assay, respectively. Finally, new high oleic and low linolenic winter rapeseed recombinant lines were obtained for use as a starting material for the development of new varieties that may be of high value on the oil crop market. |
| doi_str_mv | 10.1371/journal.pone.0233959 |
| format | Article |
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Such kind of oil is ideal for deep frying and can also be used as a raw material for biodiesel production. By performing chemical mutagenesis using ethyl methanesulfonate, we obtained mutant winter rapeseed breeding lines that can produce oil with a high content of oleic acid (C18:1, more than 75%) and a low content of linolenic acid (C18:3, less than 3%). However, the mutant lines revealed low agricultural value as they were characterized by low seed yield, low wintering, and high content of glucosinolates in seed meal. The aim of this work was to improve the mutant lines and develop high-oleic and low-linolenic recombinants exhibiting both good oil quality and high agronomic value. The plant materials used in this study included high-oleic and low-linolenic mutant breeding lines and high-yielding domestic canola-type breeding lines of good agricultural value with high oleic acid content and extremely low glucosinolates content. Field trials were conducted in four environments, in a randomized complete block design. Phenotyping was performed for wintering, yield of seed and oil, and seed quality traits. Genotype x environment interaction was investigated with respect to the content of C18:1 and C18:3 acids in seed oil. Genotyping was done for the selection of homozygous high oleic and low linolenic lines using allele-specific CAPS markers and SNaPshot assay, respectively. Finally, new high oleic and low linolenic winter rapeseed recombinant lines were obtained for use as a starting material for the development of new varieties that may be of high value on the oil crop market.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0233959</identifier><identifier>PMID: 32497146</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Agricultural development ; Agricultural industry ; Agronomy ; Biodiesel fuels ; Biofuels ; Biology and Life Sciences ; Brassica ; Brassica napus ; Breeding ; Canola ; Chemical mutagenesis ; Chemical properties ; Composition ; Deoxyribonucleic acid ; DNA ; Ethyl methanesulfonate ; Fatty acids ; Frying ; Genetic aspects ; Genetics ; Genotype & phenotype ; Genotype-environment interactions ; Genotypes ; Genotyping ; Glucosinolates ; Inbreeding ; Linolenic acid ; Linolenic acids ; Lipid synthesis ; Marker-assisted selection ; Markers ; Methods ; Monounsaturated fatty acids ; Mutagenesis ; Mutants ; Nutrition ; Oils & fats ; Oilseed crops ; Oilseeds ; Oleic acid ; Phenotyping ; Physical Sciences ; Plant breeding ; Production processes ; Rape (Plant) ; Rape plants ; Rapeseed ; Rapeseed oil ; Raw materials ; Recombinants ; Seed meal ; Seeds ; Unsaturated fatty acids ; Winter</subject><ispartof>PloS one, 2020-06, Vol.15 (6), p.e0233959</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Spasibionek et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Spasibionek et al 2020 Spasibionek et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c570t-7c42d5772df23ef56e63a9cf5a3dcb0e3000b3509cfdfde32190068168369843</citedby><cites>FETCH-LOGICAL-c570t-7c42d5772df23ef56e63a9cf5a3dcb0e3000b3509cfdfde32190068168369843</cites><orcidid>0000-0001-9113-567X ; 0000-0002-5874-2213</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7272079/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7272079/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids></links><search><contributor>Li, Maoteng</contributor><creatorcontrib>Spasibionek, Stanislaw</creatorcontrib><creatorcontrib>Mikolajczyk, Katarzyna</creatorcontrib><creatorcontrib>Cwiek-Kupczynska, Hanna</creatorcontrib><creatorcontrib>Pietka, Teresa</creatorcontrib><creatorcontrib>Krotka, Krystyna</creatorcontrib><creatorcontrib>Matuszczak, Marcin</creatorcontrib><creatorcontrib>Nowakowska, Joanna</creatorcontrib><creatorcontrib>Michalski, Krzysztof</creatorcontrib><creatorcontrib>Bartkowiak-Broda, Iwona</creatorcontrib><title>Marker assisted selection of new high oleic and low linolenic winter oilseed rape (Brassica napus L.) inbred lines revealing good agricultural value</title><title>PloS one</title><description>Development of oilseed rape (Brassica napus L.) breeding lines producing oil characterized by high oleic and low linolenic acid content is an important goal of rapeseed breeding programs worldwide. Such kind of oil is ideal for deep frying and can also be used as a raw material for biodiesel production. By performing chemical mutagenesis using ethyl methanesulfonate, we obtained mutant winter rapeseed breeding lines that can produce oil with a high content of oleic acid (C18:1, more than 75%) and a low content of linolenic acid (C18:3, less than 3%). However, the mutant lines revealed low agricultural value as they were characterized by low seed yield, low wintering, and high content of glucosinolates in seed meal. The aim of this work was to improve the mutant lines and develop high-oleic and low-linolenic recombinants exhibiting both good oil quality and high agronomic value. The plant materials used in this study included high-oleic and low-linolenic mutant breeding lines and high-yielding domestic canola-type breeding lines of good agricultural value with high oleic acid content and extremely low glucosinolates content. Field trials were conducted in four environments, in a randomized complete block design. Phenotyping was performed for wintering, yield of seed and oil, and seed quality traits. Genotype x environment interaction was investigated with respect to the content of C18:1 and C18:3 acids in seed oil. Genotyping was done for the selection of homozygous high oleic and low linolenic lines using allele-specific CAPS markers and SNaPshot assay, respectively. Finally, new high oleic and low linolenic winter rapeseed recombinant lines were obtained for use as a starting material for the development of new varieties that may be of high value on the oil crop market.</description><subject>Agricultural development</subject><subject>Agricultural industry</subject><subject>Agronomy</subject><subject>Biodiesel fuels</subject><subject>Biofuels</subject><subject>Biology and Life Sciences</subject><subject>Brassica</subject><subject>Brassica napus</subject><subject>Breeding</subject><subject>Canola</subject><subject>Chemical mutagenesis</subject><subject>Chemical properties</subject><subject>Composition</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Ethyl methanesulfonate</subject><subject>Fatty acids</subject><subject>Frying</subject><subject>Genetic aspects</subject><subject>Genetics</subject><subject>Genotype & phenotype</subject><subject>Genotype-environment interactions</subject><subject>Genotypes</subject><subject>Genotyping</subject><subject>Glucosinolates</subject><subject>Inbreeding</subject><subject>Linolenic acid</subject><subject>Linolenic acids</subject><subject>Lipid synthesis</subject><subject>Marker-assisted selection</subject><subject>Markers</subject><subject>Methods</subject><subject>Monounsaturated fatty acids</subject><subject>Mutagenesis</subject><subject>Mutants</subject><subject>Nutrition</subject><subject>Oils & fats</subject><subject>Oilseed crops</subject><subject>Oilseeds</subject><subject>Oleic acid</subject><subject>Phenotyping</subject><subject>Physical Sciences</subject><subject>Plant breeding</subject><subject>Production processes</subject><subject>Rape (Plant)</subject><subject>Rape plants</subject><subject>Rapeseed</subject><subject>Rapeseed oil</subject><subject>Raw materials</subject><subject>Recombinants</subject><subject>Seed meal</subject><subject>Seeds</subject><subject>Unsaturated fatty 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assisted selection of new high oleic and low linolenic winter oilseed rape (Brassica napus L.) inbred lines revealing good agricultural value</title><author>Spasibionek, Stanislaw ; Mikolajczyk, Katarzyna ; Cwiek-Kupczynska, Hanna ; Pietka, Teresa ; Krotka, Krystyna ; Matuszczak, Marcin ; Nowakowska, Joanna ; Michalski, Krzysztof ; Bartkowiak-Broda, Iwona</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c570t-7c42d5772df23ef56e63a9cf5a3dcb0e3000b3509cfdfde32190068168369843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Agricultural development</topic><topic>Agricultural industry</topic><topic>Agronomy</topic><topic>Biodiesel fuels</topic><topic>Biofuels</topic><topic>Biology and Life Sciences</topic><topic>Brassica</topic><topic>Brassica napus</topic><topic>Breeding</topic><topic>Canola</topic><topic>Chemical mutagenesis</topic><topic>Chemical 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linolenic acid content is an important goal of rapeseed breeding programs worldwide. Such kind of oil is ideal for deep frying and can also be used as a raw material for biodiesel production. By performing chemical mutagenesis using ethyl methanesulfonate, we obtained mutant winter rapeseed breeding lines that can produce oil with a high content of oleic acid (C18:1, more than 75%) and a low content of linolenic acid (C18:3, less than 3%). However, the mutant lines revealed low agricultural value as they were characterized by low seed yield, low wintering, and high content of glucosinolates in seed meal. The aim of this work was to improve the mutant lines and develop high-oleic and low-linolenic recombinants exhibiting both good oil quality and high agronomic value. The plant materials used in this study included high-oleic and low-linolenic mutant breeding lines and high-yielding domestic canola-type breeding lines of good agricultural value with high oleic acid content and extremely low glucosinolates content. Field trials were conducted in four environments, in a randomized complete block design. Phenotyping was performed for wintering, yield of seed and oil, and seed quality traits. Genotype x environment interaction was investigated with respect to the content of C18:1 and C18:3 acids in seed oil. Genotyping was done for the selection of homozygous high oleic and low linolenic lines using allele-specific CAPS markers and SNaPshot assay, respectively. Finally, new high oleic and low linolenic winter rapeseed recombinant lines were obtained for use as a starting material for the development of new varieties that may be of high value on the oil crop market.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>32497146</pmid><doi>10.1371/journal.pone.0233959</doi><orcidid>https://orcid.org/0000-0001-9113-567X</orcidid><orcidid>https://orcid.org/0000-0002-5874-2213</orcidid><oa>free_for_read</oa></addata></record> |
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| language | eng |
| recordid | cdi_plos_journals_2409620452 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Agricultural development Agricultural industry Agronomy Biodiesel fuels Biofuels Biology and Life Sciences Brassica Brassica napus Breeding Canola Chemical mutagenesis Chemical properties Composition Deoxyribonucleic acid DNA Ethyl methanesulfonate Fatty acids Frying Genetic aspects Genetics Genotype & phenotype Genotype-environment interactions Genotypes Genotyping Glucosinolates Inbreeding Linolenic acid Linolenic acids Lipid synthesis Marker-assisted selection Markers Methods Monounsaturated fatty acids Mutagenesis Mutants Nutrition Oils & fats Oilseed crops Oilseeds Oleic acid Phenotyping Physical Sciences Plant breeding Production processes Rape (Plant) Rape plants Rapeseed Rapeseed oil Raw materials Recombinants Seed meal Seeds Unsaturated fatty acids Winter |
| title | Marker assisted selection of new high oleic and low linolenic winter oilseed rape (Brassica napus L.) inbred lines revealing good agricultural value |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T06%3A45%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Marker%20assisted%20selection%20of%20new%20high%20oleic%20and%20low%20linolenic%20winter%20oilseed%20rape%20(Brassica%20napus%20L.)%20inbred%20lines%20revealing%20good%20agricultural%20value&rft.jtitle=PloS%20one&rft.au=Spasibionek,%20Stanislaw&rft.date=2020-06-04&rft.volume=15&rft.issue=6&rft.spage=e0233959&rft.pages=e0233959-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0233959&rft_dat=%3Cgale_plos_%3EA625751178%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2409620452&rft_id=info:pmid/32497146&rft_galeid=A625751178&rft_doaj_id=oai_doaj_org_article_41dd685a93c740dd9380eb02f6ab92eb&rfr_iscdi=true |