WY7 is a newly identified promoter from the rubber powdery mildew pathogen that regulates exogenous gene expression in both monocots and dicots
Promoters are very important for transcriptional regulation and gene expression, and have become invaluable tools for genetic engineering. Owing to the characteristics of obligate biotrophs, molecular research into obligate biotrophic fungi is seriously lagging behind, and very few of their endogeno...
Gespeichert in:
| Veröffentlicht in: | PloS one 2020-06, Vol.15 (6), p.e0233911-e0233911 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0233911 |
|---|---|
| container_issue | 6 |
| container_start_page | e0233911 |
| container_title | PloS one |
| container_volume | 15 |
| creator | Wang, Yi Wang, Chen Rajaofera, Mamy Jayne Nelly Zhu, Li Liu, Wenbo Zheng, Fucong Miao, Weiguo |
| description | Promoters are very important for transcriptional regulation and gene expression, and have become invaluable tools for genetic engineering. Owing to the characteristics of obligate biotrophs, molecular research into obligate biotrophic fungi is seriously lagging behind, and very few of their endogenous promoters have been developed. In this study, a WY7 fragment was predicted in the genome of Oidium heveae Steinmann using PromoterScan. Its promoter function was verified with transient transformations (Agrobacterium tumefaciens-mediated transformation, ATMT) in Nicotiana tabacum cv. Xanthi nc. The analysis of the transcription range showed that WY7 could regulate GUS expression in both monocots (Zea mays Linn and Oryza sativa L. spp. Japonica cv. Nipponbare) and dicots (N. tabacum and Hylocereus undulates Britt). The results of the quantitative detection showed that the GUS transient expression levels when regulated by WY7 was more than 11.7 times that of the CaMV 35S promoter in dicots (N. tabacum) and 5.13 times that of the ACT1 promoter in monocots (O. sativa). GUS staining was not detected in the T1 generation of the WY7-GUS transgenic N. tabacum. This showed that WY7 is an inducible promoter. The cis elements of WY7 were predicted using PlantCARE, and further experiments indicated that WY7 was a low temperature- and salt-inducible promoter. Soluble proteins produced by WY7-hpa1Xoo transgenic tobacco elicited hypersensitive responses (HR) in N. tabacum leaves. N. tabacum transformed with pBI121-WY7-hpa1Xoo exhibited enhanced resistance to the tobacco mosaic virus (TMV). The WY7 promoter has a lot of potential as a tool for plant genetic engineering. Further in-depth studies will help to better understand the transcriptional regulation mechanisms of O. heveae. |
| doi_str_mv | 10.1371/journal.pone.0233911 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2408524484</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A625464926</galeid><doaj_id>oai_doaj_org_article_baaccf79437142f1b455b2e8a22f47e7</doaj_id><sourcerecordid>A625464926</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-749ebd50c6438231a811fcd3fe5023fd65cd3358e466c56bca3e472e9bacf4813</originalsourceid><addsrcrecordid>eNqNk9tu1DAQhiMEolB4AwSWkBBc7BIf4iQ3SFXFoVKlShzFleU4411XiR1sh7ZPwSsz226rLuoFyoVnJt_MxP9kiuIZLZeU1_TtaZij18NyCh6WJeO8pfRe8Yi2nC0kK_n9W_Ze8Til07KseCPlw2KPM1G3VVU-Kv78-FkTl4gmHs6GC-J68NlZBz2ZYhhDhkgsGiSvgcS569CfwlkP8YKMbujhjEw6r8MKPCI6kwiredAZEoHzTTTMieAB6E4RUnLBE-dJF_KajMEHEzJ29z3p3cZ8UjywekjwdHvuF98-vP96-GlxfPLx6PDgeGFky_KiFi10fVUaKXjDONUNpdb03EKFUtheVujwqgEhpalkZzQHUTNoO22saCjfL15c1Z2GkNRWy6SYKJuKCdEIJI6uiD7oUzVFN-p4oYJ26jIQ4krpmJ0ZQHVaG2PrVuBcBLO0E1XVMWg0Y1bUUGOtd9tuczdCb1DjqIedortvvFurVfitaia5pCUWeL0tEMOvGVJWo0sGhkF7QIUvvxt1KEWL6Mt_0Ltvt6VWGi_gvA3Y12yKqgPJKiFFi633i-UdFD49jDguD9ZhfCfhzU4CMhnO80rPKamjL5__nz35vsu-usWuQQ95ncIwZ_yd0i4orkATQ0oR7I3ItFSbvblWQ232Rm33BtOe3x7QTdL1ovC_9bAVcw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2408524484</pqid></control><display><type>article</type><title>WY7 is a newly identified promoter from the rubber powdery mildew pathogen that regulates exogenous gene expression in both monocots and dicots</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Wang, Yi ; Wang, Chen ; Rajaofera, Mamy Jayne Nelly ; Zhu, Li ; Liu, Wenbo ; Zheng, Fucong ; Miao, Weiguo</creator><contributor>Wang, Zonghua</contributor><creatorcontrib>Wang, Yi ; Wang, Chen ; Rajaofera, Mamy Jayne Nelly ; Zhu, Li ; Liu, Wenbo ; Zheng, Fucong ; Miao, Weiguo ; Wang, Zonghua</creatorcontrib><description>Promoters are very important for transcriptional regulation and gene expression, and have become invaluable tools for genetic engineering. Owing to the characteristics of obligate biotrophs, molecular research into obligate biotrophic fungi is seriously lagging behind, and very few of their endogenous promoters have been developed. In this study, a WY7 fragment was predicted in the genome of Oidium heveae Steinmann using PromoterScan. Its promoter function was verified with transient transformations (Agrobacterium tumefaciens-mediated transformation, ATMT) in Nicotiana tabacum cv. Xanthi nc. The analysis of the transcription range showed that WY7 could regulate GUS expression in both monocots (Zea mays Linn and Oryza sativa L. spp. Japonica cv. Nipponbare) and dicots (N. tabacum and Hylocereus undulates Britt). The results of the quantitative detection showed that the GUS transient expression levels when regulated by WY7 was more than 11.7 times that of the CaMV 35S promoter in dicots (N. tabacum) and 5.13 times that of the ACT1 promoter in monocots (O. sativa). GUS staining was not detected in the T1 generation of the WY7-GUS transgenic N. tabacum. This showed that WY7 is an inducible promoter. The cis elements of WY7 were predicted using PlantCARE, and further experiments indicated that WY7 was a low temperature- and salt-inducible promoter. Soluble proteins produced by WY7-hpa1Xoo transgenic tobacco elicited hypersensitive responses (HR) in N. tabacum leaves. N. tabacum transformed with pBI121-WY7-hpa1Xoo exhibited enhanced resistance to the tobacco mosaic virus (TMV). The WY7 promoter has a lot of potential as a tool for plant genetic engineering. Further in-depth studies will help to better understand the transcriptional regulation mechanisms of O. heveae.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0233911</identifier><identifier>PMID: 32479550</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Airborne microorganisms ; Analysis ; Biochemistry ; Biology and Life Sciences ; Computer and Information Sciences ; Corn ; Disease prevention ; Disease resistance ; Diseases and pests ; E coli ; Education ; Engineering and Technology ; Fungi ; Fungi - genetics ; Fungi - pathogenicity ; Gene expression ; Gene Expression Regulation, Fungal ; Gene regulation ; Genes ; Genetic aspects ; Genetic engineering ; Genetic Engineering - methods ; Genetic regulation ; Genetic transformation ; Genetically altered foods ; Genetically modified organisms ; Genome, Fungal ; Genomes ; Genomics ; Hevea - genetics ; Hevea - microbiology ; Host-Pathogen Interactions - genetics ; Identification and classification ; Laboratories ; Liliopsida ; Low temperature ; Magnoliopsida ; Magnoliopsida - genetics ; Magnoliopsida - microbiology ; Medicine and Health Sciences ; Nicotiana - genetics ; Nicotiana - microbiology ; Nicotiana tabacum ; Oryza - genetics ; Oryza - microbiology ; Oryza sativa ; Pathogens ; Phytopathogenic fungi ; Plant diseases ; Plant Diseases - microbiology ; Plant Diseases - prevention & control ; Plant genetic engineering ; Plant Leaves - microbiology ; Plants, Genetically Modified ; Powdery mildew ; Promoter Regions, Genetic ; Promoters ; Promoters (Genetics) ; Proteins ; Rubber ; Seeds ; Tobacco ; Tobacco (Plant) ; Transcription ; Transcription (Genetics) ; Transformation, Genetic ; Transgenic plants ; Viruses ; Wang, Chen ; Zea mays - genetics ; Zea mays - microbiology</subject><ispartof>PloS one, 2020-06, Vol.15 (6), p.e0233911-e0233911</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Wang et al 2020 Wang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-749ebd50c6438231a811fcd3fe5023fd65cd3358e466c56bca3e472e9bacf4813</citedby><cites>FETCH-LOGICAL-c692t-749ebd50c6438231a811fcd3fe5023fd65cd3358e466c56bca3e472e9bacf4813</cites><orcidid>0000-0001-8991-5511</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263610/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263610/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32479550$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Wang, Zonghua</contributor><creatorcontrib>Wang, Yi</creatorcontrib><creatorcontrib>Wang, Chen</creatorcontrib><creatorcontrib>Rajaofera, Mamy Jayne Nelly</creatorcontrib><creatorcontrib>Zhu, Li</creatorcontrib><creatorcontrib>Liu, Wenbo</creatorcontrib><creatorcontrib>Zheng, Fucong</creatorcontrib><creatorcontrib>Miao, Weiguo</creatorcontrib><title>WY7 is a newly identified promoter from the rubber powdery mildew pathogen that regulates exogenous gene expression in both monocots and dicots</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Promoters are very important for transcriptional regulation and gene expression, and have become invaluable tools for genetic engineering. Owing to the characteristics of obligate biotrophs, molecular research into obligate biotrophic fungi is seriously lagging behind, and very few of their endogenous promoters have been developed. In this study, a WY7 fragment was predicted in the genome of Oidium heveae Steinmann using PromoterScan. Its promoter function was verified with transient transformations (Agrobacterium tumefaciens-mediated transformation, ATMT) in Nicotiana tabacum cv. Xanthi nc. The analysis of the transcription range showed that WY7 could regulate GUS expression in both monocots (Zea mays Linn and Oryza sativa L. spp. Japonica cv. Nipponbare) and dicots (N. tabacum and Hylocereus undulates Britt). The results of the quantitative detection showed that the GUS transient expression levels when regulated by WY7 was more than 11.7 times that of the CaMV 35S promoter in dicots (N. tabacum) and 5.13 times that of the ACT1 promoter in monocots (O. sativa). GUS staining was not detected in the T1 generation of the WY7-GUS transgenic N. tabacum. This showed that WY7 is an inducible promoter. The cis elements of WY7 were predicted using PlantCARE, and further experiments indicated that WY7 was a low temperature- and salt-inducible promoter. Soluble proteins produced by WY7-hpa1Xoo transgenic tobacco elicited hypersensitive responses (HR) in N. tabacum leaves. N. tabacum transformed with pBI121-WY7-hpa1Xoo exhibited enhanced resistance to the tobacco mosaic virus (TMV). The WY7 promoter has a lot of potential as a tool for plant genetic engineering. Further in-depth studies will help to better understand the transcriptional regulation mechanisms of O. heveae.</description><subject>Airborne microorganisms</subject><subject>Analysis</subject><subject>Biochemistry</subject><subject>Biology and Life Sciences</subject><subject>Computer and Information Sciences</subject><subject>Corn</subject><subject>Disease prevention</subject><subject>Disease resistance</subject><subject>Diseases and pests</subject><subject>E coli</subject><subject>Education</subject><subject>Engineering and Technology</subject><subject>Fungi</subject><subject>Fungi - genetics</subject><subject>Fungi - pathogenicity</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Gene regulation</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetic engineering</subject><subject>Genetic Engineering - methods</subject><subject>Genetic regulation</subject><subject>Genetic transformation</subject><subject>Genetically altered foods</subject><subject>Genetically modified organisms</subject><subject>Genome, Fungal</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Hevea - genetics</subject><subject>Hevea - microbiology</subject><subject>Host-Pathogen Interactions - genetics</subject><subject>Identification and classification</subject><subject>Laboratories</subject><subject>Liliopsida</subject><subject>Low temperature</subject><subject>Magnoliopsida</subject><subject>Magnoliopsida - genetics</subject><subject>Magnoliopsida - microbiology</subject><subject>Medicine and Health Sciences</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - microbiology</subject><subject>Nicotiana tabacum</subject><subject>Oryza - genetics</subject><subject>Oryza - microbiology</subject><subject>Oryza sativa</subject><subject>Pathogens</subject><subject>Phytopathogenic fungi</subject><subject>Plant diseases</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Diseases - prevention & control</subject><subject>Plant genetic engineering</subject><subject>Plant Leaves - microbiology</subject><subject>Plants, Genetically Modified</subject><subject>Powdery mildew</subject><subject>Promoter Regions, Genetic</subject><subject>Promoters</subject><subject>Promoters (Genetics)</subject><subject>Proteins</subject><subject>Rubber</subject><subject>Seeds</subject><subject>Tobacco</subject><subject>Tobacco (Plant)</subject><subject>Transcription</subject><subject>Transcription (Genetics)</subject><subject>Transformation, Genetic</subject><subject>Transgenic plants</subject><subject>Viruses</subject><subject>Wang, Chen</subject><subject>Zea mays - genetics</subject><subject>Zea mays - microbiology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tu1DAQhiMEolB4AwSWkBBc7BIf4iQ3SFXFoVKlShzFleU4411XiR1sh7ZPwSsz226rLuoFyoVnJt_MxP9kiuIZLZeU1_TtaZij18NyCh6WJeO8pfRe8Yi2nC0kK_n9W_Ze8Til07KseCPlw2KPM1G3VVU-Kv78-FkTl4gmHs6GC-J68NlZBz2ZYhhDhkgsGiSvgcS569CfwlkP8YKMbujhjEw6r8MKPCI6kwiredAZEoHzTTTMieAB6E4RUnLBE-dJF_KajMEHEzJ29z3p3cZ8UjywekjwdHvuF98-vP96-GlxfPLx6PDgeGFky_KiFi10fVUaKXjDONUNpdb03EKFUtheVujwqgEhpalkZzQHUTNoO22saCjfL15c1Z2GkNRWy6SYKJuKCdEIJI6uiD7oUzVFN-p4oYJ26jIQ4krpmJ0ZQHVaG2PrVuBcBLO0E1XVMWg0Y1bUUGOtd9tuczdCb1DjqIedortvvFurVfitaia5pCUWeL0tEMOvGVJWo0sGhkF7QIUvvxt1KEWL6Mt_0Ltvt6VWGi_gvA3Y12yKqgPJKiFFi633i-UdFD49jDguD9ZhfCfhzU4CMhnO80rPKamjL5__nz35vsu-usWuQQ95ncIwZ_yd0i4orkATQ0oR7I3ItFSbvblWQ232Rm33BtOe3x7QTdL1ovC_9bAVcw</recordid><startdate>20200601</startdate><enddate>20200601</enddate><creator>Wang, Yi</creator><creator>Wang, Chen</creator><creator>Rajaofera, Mamy Jayne Nelly</creator><creator>Zhu, Li</creator><creator>Liu, Wenbo</creator><creator>Zheng, Fucong</creator><creator>Miao, Weiguo</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-8991-5511</orcidid></search><sort><creationdate>20200601</creationdate><title>WY7 is a newly identified promoter from the rubber powdery mildew pathogen that regulates exogenous gene expression in both monocots and dicots</title><author>Wang, Yi ; Wang, Chen ; Rajaofera, Mamy Jayne Nelly ; Zhu, Li ; Liu, Wenbo ; Zheng, Fucong ; Miao, Weiguo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-749ebd50c6438231a811fcd3fe5023fd65cd3358e466c56bca3e472e9bacf4813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Airborne microorganisms</topic><topic>Analysis</topic><topic>Biochemistry</topic><topic>Biology and Life Sciences</topic><topic>Computer and Information Sciences</topic><topic>Corn</topic><topic>Disease prevention</topic><topic>Disease resistance</topic><topic>Diseases and pests</topic><topic>E coli</topic><topic>Education</topic><topic>Engineering and Technology</topic><topic>Fungi</topic><topic>Fungi - genetics</topic><topic>Fungi - pathogenicity</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Gene regulation</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genetic engineering</topic><topic>Genetic Engineering - methods</topic><topic>Genetic regulation</topic><topic>Genetic transformation</topic><topic>Genetically altered foods</topic><topic>Genetically modified organisms</topic><topic>Genome, Fungal</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Hevea - genetics</topic><topic>Hevea - microbiology</topic><topic>Host-Pathogen Interactions - genetics</topic><topic>Identification and classification</topic><topic>Laboratories</topic><topic>Liliopsida</topic><topic>Low temperature</topic><topic>Magnoliopsida</topic><topic>Magnoliopsida - genetics</topic><topic>Magnoliopsida - microbiology</topic><topic>Medicine and Health Sciences</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - microbiology</topic><topic>Nicotiana tabacum</topic><topic>Oryza - genetics</topic><topic>Oryza - microbiology</topic><topic>Oryza sativa</topic><topic>Pathogens</topic><topic>Phytopathogenic fungi</topic><topic>Plant diseases</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Diseases - prevention & control</topic><topic>Plant genetic engineering</topic><topic>Plant Leaves - microbiology</topic><topic>Plants, Genetically Modified</topic><topic>Powdery mildew</topic><topic>Promoter Regions, Genetic</topic><topic>Promoters</topic><topic>Promoters (Genetics)</topic><topic>Proteins</topic><topic>Rubber</topic><topic>Seeds</topic><topic>Tobacco</topic><topic>Tobacco (Plant)</topic><topic>Transcription</topic><topic>Transcription (Genetics)</topic><topic>Transformation, Genetic</topic><topic>Transgenic plants</topic><topic>Viruses</topic><topic>Wang, Chen</topic><topic>Zea mays - genetics</topic><topic>Zea mays - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yi</creatorcontrib><creatorcontrib>Wang, Chen</creatorcontrib><creatorcontrib>Rajaofera, Mamy Jayne Nelly</creatorcontrib><creatorcontrib>Zhu, Li</creatorcontrib><creatorcontrib>Liu, Wenbo</creatorcontrib><creatorcontrib>Zheng, Fucong</creatorcontrib><creatorcontrib>Miao, Weiguo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yi</au><au>Wang, Chen</au><au>Rajaofera, Mamy Jayne Nelly</au><au>Zhu, Li</au><au>Liu, Wenbo</au><au>Zheng, Fucong</au><au>Miao, Weiguo</au><au>Wang, Zonghua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>WY7 is a newly identified promoter from the rubber powdery mildew pathogen that regulates exogenous gene expression in both monocots and dicots</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2020-06-01</date><risdate>2020</risdate><volume>15</volume><issue>6</issue><spage>e0233911</spage><epage>e0233911</epage><pages>e0233911-e0233911</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Promoters are very important for transcriptional regulation and gene expression, and have become invaluable tools for genetic engineering. Owing to the characteristics of obligate biotrophs, molecular research into obligate biotrophic fungi is seriously lagging behind, and very few of their endogenous promoters have been developed. In this study, a WY7 fragment was predicted in the genome of Oidium heveae Steinmann using PromoterScan. Its promoter function was verified with transient transformations (Agrobacterium tumefaciens-mediated transformation, ATMT) in Nicotiana tabacum cv. Xanthi nc. The analysis of the transcription range showed that WY7 could regulate GUS expression in both monocots (Zea mays Linn and Oryza sativa L. spp. Japonica cv. Nipponbare) and dicots (N. tabacum and Hylocereus undulates Britt). The results of the quantitative detection showed that the GUS transient expression levels when regulated by WY7 was more than 11.7 times that of the CaMV 35S promoter in dicots (N. tabacum) and 5.13 times that of the ACT1 promoter in monocots (O. sativa). GUS staining was not detected in the T1 generation of the WY7-GUS transgenic N. tabacum. This showed that WY7 is an inducible promoter. The cis elements of WY7 were predicted using PlantCARE, and further experiments indicated that WY7 was a low temperature- and salt-inducible promoter. Soluble proteins produced by WY7-hpa1Xoo transgenic tobacco elicited hypersensitive responses (HR) in N. tabacum leaves. N. tabacum transformed with pBI121-WY7-hpa1Xoo exhibited enhanced resistance to the tobacco mosaic virus (TMV). The WY7 promoter has a lot of potential as a tool for plant genetic engineering. Further in-depth studies will help to better understand the transcriptional regulation mechanisms of O. heveae.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32479550</pmid><doi>10.1371/journal.pone.0233911</doi><tpages>e0233911</tpages><orcidid>https://orcid.org/0000-0001-8991-5511</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2020-06, Vol.15 (6), p.e0233911-e0233911 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2408524484 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Airborne microorganisms Analysis Biochemistry Biology and Life Sciences Computer and Information Sciences Corn Disease prevention Disease resistance Diseases and pests E coli Education Engineering and Technology Fungi Fungi - genetics Fungi - pathogenicity Gene expression Gene Expression Regulation, Fungal Gene regulation Genes Genetic aspects Genetic engineering Genetic Engineering - methods Genetic regulation Genetic transformation Genetically altered foods Genetically modified organisms Genome, Fungal Genomes Genomics Hevea - genetics Hevea - microbiology Host-Pathogen Interactions - genetics Identification and classification Laboratories Liliopsida Low temperature Magnoliopsida Magnoliopsida - genetics Magnoliopsida - microbiology Medicine and Health Sciences Nicotiana - genetics Nicotiana - microbiology Nicotiana tabacum Oryza - genetics Oryza - microbiology Oryza sativa Pathogens Phytopathogenic fungi Plant diseases Plant Diseases - microbiology Plant Diseases - prevention & control Plant genetic engineering Plant Leaves - microbiology Plants, Genetically Modified Powdery mildew Promoter Regions, Genetic Promoters Promoters (Genetics) Proteins Rubber Seeds Tobacco Tobacco (Plant) Transcription Transcription (Genetics) Transformation, Genetic Transgenic plants Viruses Wang, Chen Zea mays - genetics Zea mays - microbiology |
| title | WY7 is a newly identified promoter from the rubber powdery mildew pathogen that regulates exogenous gene expression in both monocots and dicots |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T15%3A09%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=WY7%20is%20a%20newly%20identified%20promoter%20from%20the%20rubber%20powdery%20mildew%20pathogen%20that%20regulates%20exogenous%20gene%20expression%20in%20both%20monocots%20and%20dicots&rft.jtitle=PloS%20one&rft.au=Wang,%20Yi&rft.date=2020-06-01&rft.volume=15&rft.issue=6&rft.spage=e0233911&rft.epage=e0233911&rft.pages=e0233911-e0233911&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0233911&rft_dat=%3Cgale_plos_%3EA625464926%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2408524484&rft_id=info:pmid/32479550&rft_galeid=A625464926&rft_doaj_id=oai_doaj_org_article_baaccf79437142f1b455b2e8a22f47e7&rfr_iscdi=true |