Phosphorylation of mouse intestinal basolateral amino acid uniporter LAT4 is controlled by food-entrained diurnal rhythm and dietary proteins
Adaptive regulation of epithelial transporters to nutrient intake is essential to decrease energy costs of their synthesis and maintenance, however such regulation is understudied. Previously we demonstrated that the transport function of the basolateral amino acid uniporter LAT4 (Slc43a2) is increa...
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| description | Adaptive regulation of epithelial transporters to nutrient intake is essential to decrease energy costs of their synthesis and maintenance, however such regulation is understudied. Previously we demonstrated that the transport function of the basolateral amino acid uniporter LAT4 (Slc43a2) is increased by dephosphorylation of serine 274 (S274) and nearly abolished by dephosphorylation of serine 297 (S297) when expressed in Xenopus oocytes. Phosphorylation changes in the jejunum of food-entrained mice suggested an increase in LAT4 transport function during food expectation. Thus, we investigated further how phosphorylation, expression and localization of mouse intestinal LAT4 respond to food-entrained diurnal rhythm and dietary protein content. In mice entrained with 18% protein diet, LAT4 mRNA was not submitted to diurnal regulation, unlike mRNAs of luminal symporters and antiporters. Only in duodenum, LAT4 protein expression increased during food intake. Concurrently, S274 phosphorylation was decreased in all three small intestinal segments, whereas S297 phosphorylation was increased only in jejunum. Interestingly, during food intake, S274 phosphorylation was nearly absent in ileum and accompanied by strong phosphorylation of mTORC1 target S6. Entraining mice with 8% protein diet provoked a shift in jejunal LAT4 localization from the cell surface to intracellular stores and increased S274 phosphorylation in both jejunum and ileum during food anticipation, suggesting decreased transport function. In contrast, 40% dietary protein content led to increased LAT4 expression in jejunum and its internalization in ileum. Ex vivo treatments of isolated intestinal villi fraction demonstrated that S274 phosphorylation was stimulated by protein kinase A. Rapamycin-sensitive insulin treatment and amino acids increased S297 phosphorylation, suggesting that the response to food intake might be regulated via the insulin-mTORC1 pathway. Ghrelin, an oscillating orexigenic hormone, did not affect phosphorylation of intestinal LAT4. Overall, we show that phosphorylation, expression and localization of intestinal mouse LAT4 responds to diurnal and dietary stimuli in location-specific manner. |
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Previously we demonstrated that the transport function of the basolateral amino acid uniporter LAT4 (Slc43a2) is increased by dephosphorylation of serine 274 (S274) and nearly abolished by dephosphorylation of serine 297 (S297) when expressed in Xenopus oocytes. Phosphorylation changes in the jejunum of food-entrained mice suggested an increase in LAT4 transport function during food expectation. Thus, we investigated further how phosphorylation, expression and localization of mouse intestinal LAT4 respond to food-entrained diurnal rhythm and dietary protein content. In mice entrained with 18% protein diet, LAT4 mRNA was not submitted to diurnal regulation, unlike mRNAs of luminal symporters and antiporters. Only in duodenum, LAT4 protein expression increased during food intake. Concurrently, S274 phosphorylation was decreased in all three small intestinal segments, whereas S297 phosphorylation was increased only in jejunum. Interestingly, during food intake, S274 phosphorylation was nearly absent in ileum and accompanied by strong phosphorylation of mTORC1 target S6. Entraining mice with 8% protein diet provoked a shift in jejunal LAT4 localization from the cell surface to intracellular stores and increased S274 phosphorylation in both jejunum and ileum during food anticipation, suggesting decreased transport function. In contrast, 40% dietary protein content led to increased LAT4 expression in jejunum and its internalization in ileum. Ex vivo treatments of isolated intestinal villi fraction demonstrated that S274 phosphorylation was stimulated by protein kinase A. Rapamycin-sensitive insulin treatment and amino acids increased S297 phosphorylation, suggesting that the response to food intake might be regulated via the insulin-mTORC1 pathway. Ghrelin, an oscillating orexigenic hormone, did not affect phosphorylation of intestinal LAT4. Overall, we show that phosphorylation, expression and localization of intestinal mouse LAT4 responds to diurnal and dietary stimuli in location-specific manner.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0233863</identifier><identifier>PMID: 32470053</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adaptation ; Amino acid metabolism ; Amino acids ; Biology and Life Sciences ; Cell surface ; Circadian rhythms ; Dephosphorylation ; Diet ; Diurnal ; Duodenum ; Energy costs ; Entrainment ; Food ; Food intake ; Gametocytes ; Ghrelin ; Hypotheses ; Ileum ; Insulin ; Internalization ; Intestine ; Jejunum ; Kinases ; Localization ; Mammals ; Medicine and Health Sciences ; mRNA ; Nutrient content ; Oocytes ; Phosphorylation ; Physiological aspects ; Physiological research ; Physiology ; Protein kinase A ; Proteins ; Rapamycin ; Research and Analysis Methods ; Rhythm ; Serine ; Small intestine ; Studies ; Transport proteins</subject><ispartof>PloS one, 2020-05, Vol.15 (5), p.e0233863-e0233863</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Oparija-Rogenmozere et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Oparija-Rogenmozere et al 2020 Oparija-Rogenmozere et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-eab4699e633912d007b1c20bb7cfcd639a026d29687b774b8abe07e8e28378993</citedby><cites>FETCH-LOGICAL-c692t-eab4699e633912d007b1c20bb7cfcd639a026d29687b774b8abe07e8e28378993</cites><orcidid>0000-0003-3250-9824</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7259769/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7259769/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32470053$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Albrecht, Urs</contributor><creatorcontrib>Oparija-Rogenmozere, Lalita</creatorcontrib><creatorcontrib>Rajendran, Anuradha</creatorcontrib><creatorcontrib>Poncet, Nadège</creatorcontrib><creatorcontrib>Camargo, Simone M R</creatorcontrib><creatorcontrib>Verrey, François</creatorcontrib><title>Phosphorylation of mouse intestinal basolateral amino acid uniporter LAT4 is controlled by food-entrained diurnal rhythm and dietary proteins</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Adaptive regulation of epithelial transporters to nutrient intake is essential to decrease energy costs of their synthesis and maintenance, however such regulation is understudied. Previously we demonstrated that the transport function of the basolateral amino acid uniporter LAT4 (Slc43a2) is increased by dephosphorylation of serine 274 (S274) and nearly abolished by dephosphorylation of serine 297 (S297) when expressed in Xenopus oocytes. Phosphorylation changes in the jejunum of food-entrained mice suggested an increase in LAT4 transport function during food expectation. Thus, we investigated further how phosphorylation, expression and localization of mouse intestinal LAT4 respond to food-entrained diurnal rhythm and dietary protein content. In mice entrained with 18% protein diet, LAT4 mRNA was not submitted to diurnal regulation, unlike mRNAs of luminal symporters and antiporters. Only in duodenum, LAT4 protein expression increased during food intake. Concurrently, S274 phosphorylation was decreased in all three small intestinal segments, whereas S297 phosphorylation was increased only in jejunum. Interestingly, during food intake, S274 phosphorylation was nearly absent in ileum and accompanied by strong phosphorylation of mTORC1 target S6. Entraining mice with 8% protein diet provoked a shift in jejunal LAT4 localization from the cell surface to intracellular stores and increased S274 phosphorylation in both jejunum and ileum during food anticipation, suggesting decreased transport function. In contrast, 40% dietary protein content led to increased LAT4 expression in jejunum and its internalization in ileum. Ex vivo treatments of isolated intestinal villi fraction demonstrated that S274 phosphorylation was stimulated by protein kinase A. Rapamycin-sensitive insulin treatment and amino acids increased S297 phosphorylation, suggesting that the response to food intake might be regulated via the insulin-mTORC1 pathway. Ghrelin, an oscillating orexigenic hormone, did not affect phosphorylation of intestinal LAT4. Overall, we show that phosphorylation, expression and localization of intestinal mouse LAT4 responds to diurnal and dietary stimuli in location-specific manner.</description><subject>Adaptation</subject><subject>Amino acid metabolism</subject><subject>Amino acids</subject><subject>Biology and Life Sciences</subject><subject>Cell surface</subject><subject>Circadian rhythms</subject><subject>Dephosphorylation</subject><subject>Diet</subject><subject>Diurnal</subject><subject>Duodenum</subject><subject>Energy costs</subject><subject>Entrainment</subject><subject>Food</subject><subject>Food intake</subject><subject>Gametocytes</subject><subject>Ghrelin</subject><subject>Hypotheses</subject><subject>Ileum</subject><subject>Insulin</subject><subject>Internalization</subject><subject>Intestine</subject><subject>Jejunum</subject><subject>Kinases</subject><subject>Localization</subject><subject>Mammals</subject><subject>Medicine and Health Sciences</subject><subject>mRNA</subject><subject>Nutrient content</subject><subject>Oocytes</subject><subject>Phosphorylation</subject><subject>Physiological aspects</subject><subject>Physiological research</subject><subject>Physiology</subject><subject>Protein kinase A</subject><subject>Proteins</subject><subject>Rapamycin</subject><subject>Research and Analysis Methods</subject><subject>Rhythm</subject><subject>Serine</subject><subject>Small intestine</subject><subject>Studies</subject><subject>Transport 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Urs</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phosphorylation of mouse intestinal basolateral amino acid uniporter LAT4 is controlled by food-entrained diurnal rhythm and dietary proteins</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2020-05-29</date><risdate>2020</risdate><volume>15</volume><issue>5</issue><spage>e0233863</spage><epage>e0233863</epage><pages>e0233863-e0233863</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Adaptive regulation of epithelial transporters to nutrient intake is essential to decrease energy costs of their synthesis and maintenance, however such regulation is understudied. Previously we demonstrated that the transport function of the basolateral amino acid uniporter LAT4 (Slc43a2) is increased by dephosphorylation of serine 274 (S274) and nearly abolished by dephosphorylation of serine 297 (S297) when expressed in Xenopus oocytes. Phosphorylation changes in the jejunum of food-entrained mice suggested an increase in LAT4 transport function during food expectation. Thus, we investigated further how phosphorylation, expression and localization of mouse intestinal LAT4 respond to food-entrained diurnal rhythm and dietary protein content. In mice entrained with 18% protein diet, LAT4 mRNA was not submitted to diurnal regulation, unlike mRNAs of luminal symporters and antiporters. Only in duodenum, LAT4 protein expression increased during food intake. Concurrently, S274 phosphorylation was decreased in all three small intestinal segments, whereas S297 phosphorylation was increased only in jejunum. Interestingly, during food intake, S274 phosphorylation was nearly absent in ileum and accompanied by strong phosphorylation of mTORC1 target S6. Entraining mice with 8% protein diet provoked a shift in jejunal LAT4 localization from the cell surface to intracellular stores and increased S274 phosphorylation in both jejunum and ileum during food anticipation, suggesting decreased transport function. In contrast, 40% dietary protein content led to increased LAT4 expression in jejunum and its internalization in ileum. Ex vivo treatments of isolated intestinal villi fraction demonstrated that S274 phosphorylation was stimulated by protein kinase A. Rapamycin-sensitive insulin treatment and amino acids increased S297 phosphorylation, suggesting that the response to food intake might be regulated via the insulin-mTORC1 pathway. Ghrelin, an oscillating orexigenic hormone, did not affect phosphorylation of intestinal LAT4. Overall, we show that phosphorylation, expression and localization of intestinal mouse LAT4 responds to diurnal and dietary stimuli in location-specific manner.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32470053</pmid><doi>10.1371/journal.pone.0233863</doi><tpages>e0233863</tpages><orcidid>https://orcid.org/0000-0003-3250-9824</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adaptation Amino acid metabolism Amino acids Biology and Life Sciences Cell surface Circadian rhythms Dephosphorylation Diet Diurnal Duodenum Energy costs Entrainment Food Food intake Gametocytes Ghrelin Hypotheses Ileum Insulin Internalization Intestine Jejunum Kinases Localization Mammals Medicine and Health Sciences mRNA Nutrient content Oocytes Phosphorylation Physiological aspects Physiological research Physiology Protein kinase A Proteins Rapamycin Research and Analysis Methods Rhythm Serine Small intestine Studies Transport proteins |
| title | Phosphorylation of mouse intestinal basolateral amino acid uniporter LAT4 is controlled by food-entrained diurnal rhythm and dietary proteins |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T11%3A58%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Phosphorylation%20of%20mouse%20intestinal%20basolateral%20amino%20acid%20uniporter%20LAT4%20is%20controlled%20by%20food-entrained%20diurnal%20rhythm%20and%20dietary%20proteins&rft.jtitle=PloS%20one&rft.au=Oparija-Rogenmozere,%20Lalita&rft.date=2020-05-29&rft.volume=15&rft.issue=5&rft.spage=e0233863&rft.epage=e0233863&rft.pages=e0233863-e0233863&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0233863&rft_dat=%3Cgale_plos_%3EA627434817%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2407761570&rft_id=info:pmid/32470053&rft_galeid=A627434817&rft_doaj_id=oai_doaj_org_article_d19e10d9c51446f3a326c129d9f4ef35&rfr_iscdi=true |