ATR-FTIR spectroscopy as adjunct method to the microscopic examination of hematoxylin and eosin-stained tissues in diagnosing lung cancer
Lung cancer remains the leading cause of cancer-related death worldwide. Since prognosis and treatment outcomes rely on fast and accurate diagnosis, there is a need for more cost-effective, sensitive, and specific method for lung cancer detection. Thus, this study aimed to determine the ability of A...
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| creator | Bangaoil, Ruth Santillan, Abegail Angeles, Lara Mae Abanilla, Lorenzo Lim, Jr, Antonio Ramos, Ma Cristina Fellizar, Allan Guevarra, Jr, Leonardo Albano, Pia Marie |
| description | Lung cancer remains the leading cause of cancer-related death worldwide. Since prognosis and treatment outcomes rely on fast and accurate diagnosis, there is a need for more cost-effective, sensitive, and specific method for lung cancer detection. Thus, this study aimed to determine the ability of ATR-FTIR in discriminating malignant from benign lung tissues and evaluate its concordance with H&E staining. Three (3) 5μm-thick sections were cut from formalin fixed paraffin embedded (FFPE) cell or tissue blocks from patients with lung lesions. The outer sections were H&E-stained and sent to two (2) pathologists to confirm the histopathologic diagnosis. The inner section was deparaffinized by standard xylene method and then subjected to ATR-FTIR analysis. Distinct spectral profiles that distinguished (p |
| doi_str_mv | 10.1371/journal.pone.0233626 |
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Since prognosis and treatment outcomes rely on fast and accurate diagnosis, there is a need for more cost-effective, sensitive, and specific method for lung cancer detection. Thus, this study aimed to determine the ability of ATR-FTIR in discriminating malignant from benign lung tissues and evaluate its concordance with H&E staining. Three (3) 5μm-thick sections were cut from formalin fixed paraffin embedded (FFPE) cell or tissue blocks from patients with lung lesions. The outer sections were H&E-stained and sent to two (2) pathologists to confirm the histopathologic diagnosis. The inner section was deparaffinized by standard xylene method and then subjected to ATR-FTIR analysis. Distinct spectral profiles that distinguished (p<0.05) one sample from another, called the "fingerprint region", were observed in five (5) peak patterns representing the amides, lipids, and nucleic acids. Principal component analysis and hierarchical cluster analysis evidently clustered the benign from malignant tissues. ATR-FTIR showed 97.73% sensitivity, 92.45% specificity, 94.85% accuracy, 91.49% positive predictive value and 98.00% negative predictive value in discriminating benign from malignant lung tissue. Further, strong agreement was observed between histopathologic readings and ATR-FTIR analysis. This study shows the potential of ATR-FTIR spectroscopy as a potential adjunct method to the gold standard, the microscopic examination of hematoxylin and eosin (H&E)-stained tissues, in diagnosing lung cancer.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0233626</identifier><identifier>PMID: 32469931</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amides ; Biology and Life Sciences ; Cluster analysis ; Cytodiagnosis ; Diagnosis ; Fourier transform infrared spectroscopy ; Fourier transforms ; Health aspects ; Hospitals ; Infrared spectroscopy ; Lipids ; Lung cancer ; Lung diseases ; Magnetic resonance imaging ; Medical diagnosis ; Medical prognosis ; Medicine and Health Sciences ; Methods ; Nucleic acids ; Paraffin ; Paraffins ; Patients ; People and Places ; Physical Sciences ; Principal components analysis ; Research and Analysis Methods ; Spectroscopy ; Spectrum analysis ; Stains & staining ; Tissues ; Xylene</subject><ispartof>PloS one, 2020-05, Vol.15 (5), p.e0233626-e0233626</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Bangaoil et al. 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Since prognosis and treatment outcomes rely on fast and accurate diagnosis, there is a need for more cost-effective, sensitive, and specific method for lung cancer detection. Thus, this study aimed to determine the ability of ATR-FTIR in discriminating malignant from benign lung tissues and evaluate its concordance with H&E staining. Three (3) 5μm-thick sections were cut from formalin fixed paraffin embedded (FFPE) cell or tissue blocks from patients with lung lesions. The outer sections were H&E-stained and sent to two (2) pathologists to confirm the histopathologic diagnosis. The inner section was deparaffinized by standard xylene method and then subjected to ATR-FTIR analysis. Distinct spectral profiles that distinguished (p<0.05) one sample from another, called the "fingerprint region", were observed in five (5) peak patterns representing the amides, lipids, and nucleic acids. 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This study shows the potential of ATR-FTIR spectroscopy as a potential adjunct method to the gold standard, the microscopic examination of hematoxylin and eosin (H&E)-stained tissues, in diagnosing lung cancer.</description><subject>Amides</subject><subject>Biology and Life Sciences</subject><subject>Cluster analysis</subject><subject>Cytodiagnosis</subject><subject>Diagnosis</subject><subject>Fourier transform infrared spectroscopy</subject><subject>Fourier transforms</subject><subject>Health aspects</subject><subject>Hospitals</subject><subject>Infrared spectroscopy</subject><subject>Lipids</subject><subject>Lung cancer</subject><subject>Lung diseases</subject><subject>Magnetic resonance imaging</subject><subject>Medical diagnosis</subject><subject>Medical prognosis</subject><subject>Medicine and Health Sciences</subject><subject>Methods</subject><subject>Nucleic acids</subject><subject>Paraffin</subject><subject>Paraffins</subject><subject>Patients</subject><subject>People and 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Yuval</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ATR-FTIR spectroscopy as adjunct method to the microscopic examination of hematoxylin and eosin-stained tissues in diagnosing lung cancer</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2020-05-29</date><risdate>2020</risdate><volume>15</volume><issue>5</issue><spage>e0233626</spage><epage>e0233626</epage><pages>e0233626-e0233626</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Lung cancer remains the leading cause of cancer-related death worldwide. Since prognosis and treatment outcomes rely on fast and accurate diagnosis, there is a need for more cost-effective, sensitive, and specific method for lung cancer detection. Thus, this study aimed to determine the ability of ATR-FTIR in discriminating malignant from benign lung tissues and evaluate its concordance with H&E staining. Three (3) 5μm-thick sections were cut from formalin fixed paraffin embedded (FFPE) cell or tissue blocks from patients with lung lesions. The outer sections were H&E-stained and sent to two (2) pathologists to confirm the histopathologic diagnosis. The inner section was deparaffinized by standard xylene method and then subjected to ATR-FTIR analysis. Distinct spectral profiles that distinguished (p<0.05) one sample from another, called the "fingerprint region", were observed in five (5) peak patterns representing the amides, lipids, and nucleic acids. Principal component analysis and hierarchical cluster analysis evidently clustered the benign from malignant tissues. ATR-FTIR showed 97.73% sensitivity, 92.45% specificity, 94.85% accuracy, 91.49% positive predictive value and 98.00% negative predictive value in discriminating benign from malignant lung tissue. Further, strong agreement was observed between histopathologic readings and ATR-FTIR analysis. This study shows the potential of ATR-FTIR spectroscopy as a potential adjunct method to the gold standard, the microscopic examination of hematoxylin and eosin (H&E)-stained tissues, in diagnosing lung cancer.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32469931</pmid><doi>10.1371/journal.pone.0233626</doi><tpages>e0233626</tpages><orcidid>https://orcid.org/0000-0002-7329-4594</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Amides Biology and Life Sciences Cluster analysis Cytodiagnosis Diagnosis Fourier transform infrared spectroscopy Fourier transforms Health aspects Hospitals Infrared spectroscopy Lipids Lung cancer Lung diseases Magnetic resonance imaging Medical diagnosis Medical prognosis Medicine and Health Sciences Methods Nucleic acids Paraffin Paraffins Patients People and Places Physical Sciences Principal components analysis Research and Analysis Methods Spectroscopy Spectrum analysis Stains & staining Tissues Xylene |
| title | ATR-FTIR spectroscopy as adjunct method to the microscopic examination of hematoxylin and eosin-stained tissues in diagnosing lung cancer |
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