Voltammetric analysis for distinguishing portal hypertension-related from malignancy-related ascites: A proof of concept study
Serum-ascites albumin gradient (SAAG) remains the most sensitive and specific marker for the differentiation of ascites due to portal hypertension from ascites due to other causes. SAAG has some limitations and may fail in selected conditions. Voltammetric analysis (VA) has been used for the detecti...
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| creator | Muley, Moises Vespasiani-Gentilucci, Umberto De Vincentis, Antonio Santonico, Marco Pennazza, Giorgio Sanguedolce, Simona De Luca, Cristiana Plotti, Francesco Picardi, Antonio Antonelli-Incalzi, Raffaele |
| description | Serum-ascites albumin gradient (SAAG) remains the most sensitive and specific marker for the differentiation of ascites due to portal hypertension from ascites due to other causes. SAAG has some limitations and may fail in selected conditions. Voltammetric analysis (VA) has been used for the detection of electroactive species of biological significance and has proven effective for detection infections in biological fluids.
In this study, we compared the accuracy of voltammetric analysis (VA) with that of SAAG to differentiate ascites due to portal hypertension from that having a different origin.
80 ascites samples were obtained from patients undergoing paracentesis at the Campus Bio-Medico Hospital of Rome. VA was performed using the BIONOTE device. The ability of VA to discriminate ascitic fluid etiology and biochemical parameters was evaluated using Partial Least Square Discriminant Analysis (PLS-DA), with ten-fold cross-validations.
Mean age was 68.6 years (SD 12.5), 58% were male. Ascites was secondary to only portal hypertension in 72.5% of cases (58 subjects) and it was secondary to a baseline neoplastic disease in 27.5% of cases (22 subjects). Compared to SAAG≥1.1, e-tongue predicted ascites from portal hypertension with a better accuracy (92.5% Vs 87.5%); sensitivity (98.3% Vs 94.8%); specificity (77.3% Vs 68.2%); predictive values (PPV 91.9% Vs 88.7% and NPV 94.4% Vs 83.3%). VA correctly classified ascites etiology in 57/58 (98.2%) of cases with portal hypertension and in 17/22 (77.2%) of cases with malignancy. Instead, VA showed poor predictive capacities towards total white blood count and polymorphonuclear cell count.
According to this proof of concept study, VA qualifies as a promising low-cost and easy method to discriminate between ascites secondary to portal hypertension and ascites due to malignancy. |
| doi_str_mv | 10.1371/journal.pone.0233350 |
| format | Article |
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In this study, we compared the accuracy of voltammetric analysis (VA) with that of SAAG to differentiate ascites due to portal hypertension from that having a different origin.
80 ascites samples were obtained from patients undergoing paracentesis at the Campus Bio-Medico Hospital of Rome. VA was performed using the BIONOTE device. The ability of VA to discriminate ascitic fluid etiology and biochemical parameters was evaluated using Partial Least Square Discriminant Analysis (PLS-DA), with ten-fold cross-validations.
Mean age was 68.6 years (SD 12.5), 58% were male. Ascites was secondary to only portal hypertension in 72.5% of cases (58 subjects) and it was secondary to a baseline neoplastic disease in 27.5% of cases (22 subjects). Compared to SAAG≥1.1, e-tongue predicted ascites from portal hypertension with a better accuracy (92.5% Vs 87.5%); sensitivity (98.3% Vs 94.8%); specificity (77.3% Vs 68.2%); predictive values (PPV 91.9% Vs 88.7% and NPV 94.4% Vs 83.3%). VA correctly classified ascites etiology in 57/58 (98.2%) of cases with portal hypertension and in 17/22 (77.2%) of cases with malignancy. Instead, VA showed poor predictive capacities towards total white blood count and polymorphonuclear cell count.
According to this proof of concept study, VA qualifies as a promising low-cost and easy method to discriminate between ascites secondary to portal hypertension and ascites due to malignancy.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0233350</identifier><identifier>PMID: 32437441</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abdomen ; Albumin ; Albumins ; Ascites ; Ascitic fluid ; Biology and Life Sciences ; Blood ; Cancer ; Causes of ; Chemical properties ; College campuses ; Complications and side effects ; Discriminant analysis ; Diseases ; Electrodes ; Electronic tongues ; Etiology ; Etiology (Medicine) ; Fluids ; Gynecology ; Heart failure ; Hepatology ; Hypertension ; Identification and classification ; Infection ; Internal medicine ; Liver cirrhosis ; Malignancy ; Medical diagnosis ; Medical prognosis ; Medical research ; Medicine ; Medicine and Health Sciences ; Obstetrics ; Pancreatitis ; Pathology ; Portal hypertension ; Proteins ; Research and Analysis Methods ; Sensors ; Tuberculosis ; Ultrasonic imaging ; Veterans hospitals ; Voltammetry</subject><ispartof>PloS one, 2020-05, Vol.15 (5), p.e0233350-e0233350</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Muley et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Muley et al 2020 Muley et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-93e143bdd9847b44432a24ab665d9ee7a63cfe853a60b24cd187f22ee15dc70f3</citedby><cites>FETCH-LOGICAL-c692t-93e143bdd9847b44432a24ab665d9ee7a63cfe853a60b24cd187f22ee15dc70f3</cites><orcidid>0000-0001-9723-7116</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241828/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241828/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32437441$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Avila, Matias A.</contributor><creatorcontrib>Muley, Moises</creatorcontrib><creatorcontrib>Vespasiani-Gentilucci, Umberto</creatorcontrib><creatorcontrib>De Vincentis, Antonio</creatorcontrib><creatorcontrib>Santonico, Marco</creatorcontrib><creatorcontrib>Pennazza, Giorgio</creatorcontrib><creatorcontrib>Sanguedolce, Simona</creatorcontrib><creatorcontrib>De Luca, Cristiana</creatorcontrib><creatorcontrib>Plotti, Francesco</creatorcontrib><creatorcontrib>Picardi, Antonio</creatorcontrib><creatorcontrib>Antonelli-Incalzi, Raffaele</creatorcontrib><title>Voltammetric analysis for distinguishing portal hypertension-related from malignancy-related ascites: A proof of concept study</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Serum-ascites albumin gradient (SAAG) remains the most sensitive and specific marker for the differentiation of ascites due to portal hypertension from ascites due to other causes. SAAG has some limitations and may fail in selected conditions. Voltammetric analysis (VA) has been used for the detection of electroactive species of biological significance and has proven effective for detection infections in biological fluids.
In this study, we compared the accuracy of voltammetric analysis (VA) with that of SAAG to differentiate ascites due to portal hypertension from that having a different origin.
80 ascites samples were obtained from patients undergoing paracentesis at the Campus Bio-Medico Hospital of Rome. VA was performed using the BIONOTE device. The ability of VA to discriminate ascitic fluid etiology and biochemical parameters was evaluated using Partial Least Square Discriminant Analysis (PLS-DA), with ten-fold cross-validations.
Mean age was 68.6 years (SD 12.5), 58% were male. Ascites was secondary to only portal hypertension in 72.5% of cases (58 subjects) and it was secondary to a baseline neoplastic disease in 27.5% of cases (22 subjects). Compared to SAAG≥1.1, e-tongue predicted ascites from portal hypertension with a better accuracy (92.5% Vs 87.5%); sensitivity (98.3% Vs 94.8%); specificity (77.3% Vs 68.2%); predictive values (PPV 91.9% Vs 88.7% and NPV 94.4% Vs 83.3%). VA correctly classified ascites etiology in 57/58 (98.2%) of cases with portal hypertension and in 17/22 (77.2%) of cases with malignancy. Instead, VA showed poor predictive capacities towards total white blood count and polymorphonuclear cell count.
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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Muley, Moises</au><au>Vespasiani-Gentilucci, Umberto</au><au>De Vincentis, Antonio</au><au>Santonico, Marco</au><au>Pennazza, Giorgio</au><au>Sanguedolce, Simona</au><au>De Luca, Cristiana</au><au>Plotti, Francesco</au><au>Picardi, Antonio</au><au>Antonelli-Incalzi, Raffaele</au><au>Avila, Matias A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Voltammetric analysis for distinguishing portal hypertension-related from malignancy-related ascites: A proof of concept study</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2020-05-21</date><risdate>2020</risdate><volume>15</volume><issue>5</issue><spage>e0233350</spage><epage>e0233350</epage><pages>e0233350-e0233350</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Serum-ascites albumin gradient (SAAG) remains the most sensitive and specific marker for the differentiation of ascites due to portal hypertension from ascites due to other causes. SAAG has some limitations and may fail in selected conditions. Voltammetric analysis (VA) has been used for the detection of electroactive species of biological significance and has proven effective for detection infections in biological fluids.
In this study, we compared the accuracy of voltammetric analysis (VA) with that of SAAG to differentiate ascites due to portal hypertension from that having a different origin.
80 ascites samples were obtained from patients undergoing paracentesis at the Campus Bio-Medico Hospital of Rome. VA was performed using the BIONOTE device. The ability of VA to discriminate ascitic fluid etiology and biochemical parameters was evaluated using Partial Least Square Discriminant Analysis (PLS-DA), with ten-fold cross-validations.
Mean age was 68.6 years (SD 12.5), 58% were male. Ascites was secondary to only portal hypertension in 72.5% of cases (58 subjects) and it was secondary to a baseline neoplastic disease in 27.5% of cases (22 subjects). Compared to SAAG≥1.1, e-tongue predicted ascites from portal hypertension with a better accuracy (92.5% Vs 87.5%); sensitivity (98.3% Vs 94.8%); specificity (77.3% Vs 68.2%); predictive values (PPV 91.9% Vs 88.7% and NPV 94.4% Vs 83.3%). VA correctly classified ascites etiology in 57/58 (98.2%) of cases with portal hypertension and in 17/22 (77.2%) of cases with malignancy. Instead, VA showed poor predictive capacities towards total white blood count and polymorphonuclear cell count.
According to this proof of concept study, VA qualifies as a promising low-cost and easy method to discriminate between ascites secondary to portal hypertension and ascites due to malignancy.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32437441</pmid><doi>10.1371/journal.pone.0233350</doi><tpages>e0233350</tpages><orcidid>https://orcid.org/0000-0001-9723-7116</orcidid><oa>free_for_read</oa></addata></record> |
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| language | eng |
| recordid | cdi_plos_journals_2405599189 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Abdomen Albumin Albumins Ascites Ascitic fluid Biology and Life Sciences Blood Cancer Causes of Chemical properties College campuses Complications and side effects Discriminant analysis Diseases Electrodes Electronic tongues Etiology Etiology (Medicine) Fluids Gynecology Heart failure Hepatology Hypertension Identification and classification Infection Internal medicine Liver cirrhosis Malignancy Medical diagnosis Medical prognosis Medical research Medicine Medicine and Health Sciences Obstetrics Pancreatitis Pathology Portal hypertension Proteins Research and Analysis Methods Sensors Tuberculosis Ultrasonic imaging Veterans hospitals Voltammetry |
| title | Voltammetric analysis for distinguishing portal hypertension-related from malignancy-related ascites: A proof of concept study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T07%3A48%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Voltammetric%20analysis%20for%20distinguishing%20portal%20hypertension-related%20from%20malignancy-related%20ascites:%20A%20proof%20of%20concept%20study&rft.jtitle=PloS%20one&rft.au=Muley,%20Moises&rft.date=2020-05-21&rft.volume=15&rft.issue=5&rft.spage=e0233350&rft.epage=e0233350&rft.pages=e0233350-e0233350&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0233350&rft_dat=%3Cgale_plos_%3EA624573687%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2405599189&rft_id=info:pmid/32437441&rft_galeid=A624573687&rft_doaj_id=oai_doaj_org_article_8c715e8a11f54f2d8f6917ae1dcd9af0&rfr_iscdi=true |