Multiple Wolbachia strains provide comparative levels of protection against dengue virus infection in Aedes aegypti
The insect bacterium Wolbachia pipientis is being introgressed into Aedes aegypti populations as an intervention against the transmission of medically important arboviruses. Here we compare Ae. aegypti mosquitoes infected with wMelCS or wAlbB to the widely used wMel Wolbachia strain on an Australian...
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| creator | Flores, Heather A Taneja de Bruyne, Jyotika O'Donnell, Tanya B Tuyet Nhu, Vu Thi Giang, Nguyen Thi Xuan Trang, Huynh Thi Thuy Van, Huynh Thi Long, Vo Thi Dui, Le Le Anh Huy, Huynh Thi Le Duyen, Huynh Thi Van Thuy, Nguyen Thanh Phong, Nguyen Van Vinh Chau, Nguyen Thi Hue Kien, Duong Thuy Vi, Tran Wills, Bridget O'Neill, Scott L Simmons, Cameron P Carrington, Lauren B |
| description | The insect bacterium Wolbachia pipientis is being introgressed into Aedes aegypti populations as an intervention against the transmission of medically important arboviruses. Here we compare Ae. aegypti mosquitoes infected with wMelCS or wAlbB to the widely used wMel Wolbachia strain on an Australian nuclear genetic background for their susceptibility to infection by dengue virus (DENV) genotypes spanning all four serotypes. All Wolbachia-infected mosquitoes were more resistant to intrathoracic DENV challenge than their wildtype counterparts. Blocking of DENV replication was greatest by wMelCS. Conversely, wAlbB-infected mosquitoes were more susceptible to whole body infection than wMel and wMelCS. We extended these findings via mosquito oral feeding experiments, using viremic blood from 36 acute, hospitalised dengue cases in Vietnam, additionally including wMel and wildtype mosquitoes on a Vietnamese nuclear genetic background. As above, wAlbB was less effective at blocking DENV replication in the abdomen compared to wMel and wMelCS. The transmission potential of all Wolbachia-infected mosquito lines (measured by the presence/absence of infectious DENV in mosquito saliva) after 14 days, was significantly reduced compared to their wildtype counterparts, and lowest for wMelCS and wAlbB. These data support the use of wAlbB and wMelCS strains for introgression field trials and the biocontrol of DENV transmission. Furthermore, despite observing significant differences in transmission potential between wildtype mosquitoes from Australia and Vietnam, no difference was observed between wMel-infected mosquitoes from each background suggesting that Wolbachia may override any underlying variation in DENV transmission potential. |
| doi_str_mv | 10.1371/journal.ppat.1008433 |
| format | Article |
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Here we compare Ae. aegypti mosquitoes infected with wMelCS or wAlbB to the widely used wMel Wolbachia strain on an Australian nuclear genetic background for their susceptibility to infection by dengue virus (DENV) genotypes spanning all four serotypes. All Wolbachia-infected mosquitoes were more resistant to intrathoracic DENV challenge than their wildtype counterparts. Blocking of DENV replication was greatest by wMelCS. Conversely, wAlbB-infected mosquitoes were more susceptible to whole body infection than wMel and wMelCS. We extended these findings via mosquito oral feeding experiments, using viremic blood from 36 acute, hospitalised dengue cases in Vietnam, additionally including wMel and wildtype mosquitoes on a Vietnamese nuclear genetic background. As above, wAlbB was less effective at blocking DENV replication in the abdomen compared to wMel and wMelCS. The transmission potential of all Wolbachia-infected mosquito lines (measured by the presence/absence of infectious DENV in mosquito saliva) after 14 days, was significantly reduced compared to their wildtype counterparts, and lowest for wMelCS and wAlbB. These data support the use of wAlbB and wMelCS strains for introgression field trials and the biocontrol of DENV transmission. Furthermore, despite observing significant differences in transmission potential between wildtype mosquitoes from Australia and Vietnam, no difference was observed between wMel-infected mosquitoes from each background suggesting that Wolbachia may override any underlying variation in DENV transmission potential.</description><identifier>ISSN: 1553-7374</identifier><identifier>ISSN: 1553-7366</identifier><identifier>EISSN: 1553-7374</identifier><identifier>DOI: 10.1371/journal.ppat.1008433</identifier><identifier>PMID: 32282862</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aedes aegypti ; Aquatic insects ; Australia ; Biological control ; Biological pest control ; Biology and Life Sciences ; Comparative analysis ; Culicidae ; Dengue ; Dengue fever ; Dengue virus ; Disease susceptibility ; Disease transmission ; Diseases ; Genetic aspects ; Genotypes ; Health aspects ; Infection ; Infections ; Insects ; Medicine and Health Sciences ; Mosquitoes ; Replication ; Research and Analysis Methods ; Saliva ; Serotypes ; Strains (organisms) ; Tropical diseases ; Vector-borne diseases ; Vietnam ; Virus diseases ; Viruses ; Wolbachia</subject><ispartof>PLoS pathogens, 2020-04, Vol.16 (4), p.e1008433-e1008433</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Flores et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Flores et al 2020 Flores et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c727t-7dfddd5809102d470dad7fad97e06bbc241134aa3ceb2d1da1be7eeed2e040c53</citedby><cites>FETCH-LOGICAL-c727t-7dfddd5809102d470dad7fad97e06bbc241134aa3ceb2d1da1be7eeed2e040c53</cites><orcidid>0000-0001-9273-7912 ; 0000-0001-7298-5724 ; 0000-0001-9086-8804 ; 0000-0001-5003-7547 ; 0000-0003-4668-1019 ; 0000-0002-4131-3615</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179939/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179939/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32282862$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Flores, Heather A</creatorcontrib><creatorcontrib>Taneja de Bruyne, Jyotika</creatorcontrib><creatorcontrib>O'Donnell, Tanya B</creatorcontrib><creatorcontrib>Tuyet Nhu, Vu</creatorcontrib><creatorcontrib>Thi Giang, Nguyen</creatorcontrib><creatorcontrib>Thi Xuan Trang, Huynh</creatorcontrib><creatorcontrib>Thi Thuy Van, Huynh</creatorcontrib><creatorcontrib>Thi Long, Vo</creatorcontrib><creatorcontrib>Thi Dui, Le</creatorcontrib><creatorcontrib>Le Anh Huy, Huynh</creatorcontrib><creatorcontrib>Thi Le Duyen, Huynh</creatorcontrib><creatorcontrib>Thi Van Thuy, Nguyen</creatorcontrib><creatorcontrib>Thanh Phong, Nguyen</creatorcontrib><creatorcontrib>Van Vinh Chau, Nguyen</creatorcontrib><creatorcontrib>Thi Hue Kien, Duong</creatorcontrib><creatorcontrib>Thuy Vi, Tran</creatorcontrib><creatorcontrib>Wills, Bridget</creatorcontrib><creatorcontrib>O'Neill, Scott L</creatorcontrib><creatorcontrib>Simmons, Cameron P</creatorcontrib><creatorcontrib>Carrington, Lauren B</creatorcontrib><title>Multiple Wolbachia strains provide comparative levels of protection against dengue virus infection in Aedes aegypti</title><title>PLoS pathogens</title><addtitle>PLoS Pathog</addtitle><description>The insect bacterium Wolbachia pipientis is being introgressed into Aedes aegypti populations as an intervention against the transmission of medically important arboviruses. Here we compare Ae. aegypti mosquitoes infected with wMelCS or wAlbB to the widely used wMel Wolbachia strain on an Australian nuclear genetic background for their susceptibility to infection by dengue virus (DENV) genotypes spanning all four serotypes. All Wolbachia-infected mosquitoes were more resistant to intrathoracic DENV challenge than their wildtype counterparts. Blocking of DENV replication was greatest by wMelCS. Conversely, wAlbB-infected mosquitoes were more susceptible to whole body infection than wMel and wMelCS. We extended these findings via mosquito oral feeding experiments, using viremic blood from 36 acute, hospitalised dengue cases in Vietnam, additionally including wMel and wildtype mosquitoes on a Vietnamese nuclear genetic background. As above, wAlbB was less effective at blocking DENV replication in the abdomen compared to wMel and wMelCS. The transmission potential of all Wolbachia-infected mosquito lines (measured by the presence/absence of infectious DENV in mosquito saliva) after 14 days, was significantly reduced compared to their wildtype counterparts, and lowest for wMelCS and wAlbB. These data support the use of wAlbB and wMelCS strains for introgression field trials and the biocontrol of DENV transmission. Furthermore, despite observing significant differences in transmission potential between wildtype mosquitoes from Australia and Vietnam, no difference was observed between wMel-infected mosquitoes from each background suggesting that Wolbachia may override any underlying variation in DENV transmission potential.</description><subject>Aedes aegypti</subject><subject>Aquatic insects</subject><subject>Australia</subject><subject>Biological control</subject><subject>Biological pest control</subject><subject>Biology and Life Sciences</subject><subject>Comparative analysis</subject><subject>Culicidae</subject><subject>Dengue</subject><subject>Dengue fever</subject><subject>Dengue virus</subject><subject>Disease susceptibility</subject><subject>Disease transmission</subject><subject>Diseases</subject><subject>Genetic aspects</subject><subject>Genotypes</subject><subject>Health aspects</subject><subject>Infection</subject><subject>Infections</subject><subject>Insects</subject><subject>Medicine and Health Sciences</subject><subject>Mosquitoes</subject><subject>Replication</subject><subject>Research and Analysis Methods</subject><subject>Saliva</subject><subject>Serotypes</subject><subject>Strains (organisms)</subject><subject>Tropical diseases</subject><subject>Vector-borne diseases</subject><subject>Vietnam</subject><subject>Virus 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Wolbachia strains provide comparative levels of protection against dengue virus infection in Aedes aegypti</title><author>Flores, Heather A ; Taneja de Bruyne, Jyotika ; O'Donnell, Tanya B ; Tuyet Nhu, Vu ; Thi Giang, Nguyen ; Thi Xuan Trang, Huynh ; Thi Thuy Van, Huynh ; Thi Long, Vo ; Thi Dui, Le ; Le Anh Huy, Huynh ; Thi Le Duyen, Huynh ; Thi Van Thuy, Nguyen ; Thanh Phong, Nguyen ; Van Vinh Chau, Nguyen ; Thi Hue Kien, Duong ; Thuy Vi, Tran ; Wills, Bridget ; O'Neill, Scott L ; Simmons, Cameron P ; Carrington, Lauren B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c727t-7dfddd5809102d470dad7fad97e06bbc241134aa3ceb2d1da1be7eeed2e040c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aedes aegypti</topic><topic>Aquatic insects</topic><topic>Australia</topic><topic>Biological control</topic><topic>Biological pest control</topic><topic>Biology and Life 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Here we compare Ae. aegypti mosquitoes infected with wMelCS or wAlbB to the widely used wMel Wolbachia strain on an Australian nuclear genetic background for their susceptibility to infection by dengue virus (DENV) genotypes spanning all four serotypes. All Wolbachia-infected mosquitoes were more resistant to intrathoracic DENV challenge than their wildtype counterparts. Blocking of DENV replication was greatest by wMelCS. Conversely, wAlbB-infected mosquitoes were more susceptible to whole body infection than wMel and wMelCS. We extended these findings via mosquito oral feeding experiments, using viremic blood from 36 acute, hospitalised dengue cases in Vietnam, additionally including wMel and wildtype mosquitoes on a Vietnamese nuclear genetic background. As above, wAlbB was less effective at blocking DENV replication in the abdomen compared to wMel and wMelCS. The transmission potential of all Wolbachia-infected mosquito lines (measured by the presence/absence of infectious DENV in mosquito saliva) after 14 days, was significantly reduced compared to their wildtype counterparts, and lowest for wMelCS and wAlbB. These data support the use of wAlbB and wMelCS strains for introgression field trials and the biocontrol of DENV transmission. 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| fulltext | fulltext |
| identifier | ISSN: 1553-7374 |
| ispartof | PLoS pathogens, 2020-04, Vol.16 (4), p.e1008433-e1008433 |
| issn | 1553-7374 1553-7366 1553-7374 |
| language | eng |
| recordid | cdi_plos_journals_2403776405 |
| source | Public Library of Science (PLoS) Journals Open Access; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Aedes aegypti Aquatic insects Australia Biological control Biological pest control Biology and Life Sciences Comparative analysis Culicidae Dengue Dengue fever Dengue virus Disease susceptibility Disease transmission Diseases Genetic aspects Genotypes Health aspects Infection Infections Insects Medicine and Health Sciences Mosquitoes Replication Research and Analysis Methods Saliva Serotypes Strains (organisms) Tropical diseases Vector-borne diseases Vietnam Virus diseases Viruses Wolbachia |
| title | Multiple Wolbachia strains provide comparative levels of protection against dengue virus infection in Aedes aegypti |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T19%3A50%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Multiple%20Wolbachia%20strains%20provide%20comparative%20levels%20of%20protection%20against%20dengue%20virus%20infection%20in%20Aedes%20aegypti&rft.jtitle=PLoS%20pathogens&rft.au=Flores,%20Heather%20A&rft.date=2020-04-13&rft.volume=16&rft.issue=4&rft.spage=e1008433&rft.epage=e1008433&rft.pages=e1008433-e1008433&rft.issn=1553-7374&rft.eissn=1553-7374&rft_id=info:doi/10.1371/journal.ppat.1008433&rft_dat=%3Cgale_plos_%3EA622509555%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2403776405&rft_id=info:pmid/32282862&rft_galeid=A622509555&rft_doaj_id=oai_doaj_org_article_be7c73101fec419c80b43c2d214a2ac3&rfr_iscdi=true |