Active Notch signaling is required for arm regeneration in a brittle star

Cell signaling pathways play key roles in coordinating cellular events in development. The Notch signaling pathway is highly conserved across all multicellular animals and is known to coordinate a multitude of diverse cellular events, including proliferation, differentiation, fate specification, and...

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Veröffentlicht in:PloS one 2020-05, Vol.15 (5), p.e0232981-e0232981
Hauptverfasser: Mashanov, Vladimir, Akiona, Jennifer, Khoury, Maleana, Ferrier, Jacob, Reid, Robert, Machado, Denis Jacob, Zueva, Olga, Janies, Daniel
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container_title PloS one
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creator Mashanov, Vladimir
Akiona, Jennifer
Khoury, Maleana
Ferrier, Jacob
Reid, Robert
Machado, Denis Jacob
Zueva, Olga
Janies, Daniel
description Cell signaling pathways play key roles in coordinating cellular events in development. The Notch signaling pathway is highly conserved across all multicellular animals and is known to coordinate a multitude of diverse cellular events, including proliferation, differentiation, fate specification, and cell death. Specific functions of the pathway are, however, highly context-dependent and are not well characterized in post-traumatic regeneration. Here, we use a small-molecule inhibitor of the pathway (DAPT) to demonstrate that Notch signaling is required for proper arm regeneration in the brittle star Ophioderma brevispina, a highly regenerative member of the phylum Echinodermata. We also employ a transcriptome-wide gene expression analysis (RNA-seq) to characterize the downstream genes controlled by the Notch pathway in the brittle star regeneration. We demonstrate that arm regeneration involves an extensive cross-talk between the Notch pathway and other cell signaling pathways. In the regrowing arm, Notch regulates the composition of the extracellular matrix, cell migration, proliferation, and apoptosis, as well as components of the innate immune response. We also show for the first time that Notch signaling regulates the activity of several transposable elements. Our data also suggests that one of the possible mechanisms through which Notch sustains its activity in the regenerating tissues is via suppression of Neuralized1.
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subjects Analysis
Apoptosis
Biochemistry
Biology
Biology and Life Sciences
Brittle stars
Cell cycle
Cell death
Cell differentiation
Cell migration
Cellular signal transduction
Crosstalk
Enzymes
Extracellular matrix
Fate
Gene expression
Genes
Genetic aspects
Genetic research
Growth
Immune response
Innate immunity
Ligands
Medicine and Health Sciences
Physiological aspects
Regeneration
Regeneration (Biology)
Ribonucleic acid
RNA
Signal transduction
Signaling
Stem cells
Time
Transcription factors
Transposons
Wildlife conservation
title Active Notch signaling is required for arm regeneration in a brittle star
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