High performance of an enzyme linked immunosorbent assay for American tegumentary leishmaniasis diagnosis with Leishmania (Viannia) braziliensis amastigotes membrane crude antigens
The diagnosis of American tegumentary leishmaniasis (ATL) still requires the design of more effective tools. Leishmania (Viannia) braziliensis is the causal agent of the 90% of Argentinean ATL cases. Considering the current knowledge, an ELISA based crude antigen (CA) for the diagnosis was designed....
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| creator | Bracamonte, María E Álvarez, Agustín Moya Sosa, Andrea M Hoyos, Carlos L Lauthier, Juan J Cajal, Silvana P Juarez, Marisa Uncos, Renato E Sánchez-Valdéz, Fernando J Acuña, Leonardo Diosque, Patricio Basombrío, Miguel A Nasser, Julio R Hashiguchi, Yoshihisa Korenaga, Masataka Barroso, Paola A Marco, Jorge D |
| description | The diagnosis of American tegumentary leishmaniasis (ATL) still requires the design of more effective tools. Leishmania (Viannia) braziliensis is the causal agent of the 90% of Argentinean ATL cases. Considering the current knowledge, an ELISA based crude antigen (CA) for the diagnosis was designed. Ninety-nine subjects diagnosed as ATL, 27 as no-ATL, and 84 donors from non-ATL-endemic areas were included in this study. The current ATL diagnosis was based four techniques, dermal smear microscopic examination (parasitological test), PCR, Leishmanin skin test, and clinical records. We obtained CA extracts from promastigotes and amastigotes from macrophage cultures of different zymodemes of endemic Leishmania species circulating in the study area. Crude antigens from the 'local' main zymodeme of L. (V.) braziliensis showed the highest reactivity against anti-Leishmania antibodies compared to the other included species. The CA of amastigotes of this zymodeme was 3.4 fold more reactive than promastigotes one. Moreover, amastigote-membrane CA (MCA) were 3.6 fold more reactive than the soluble antigens. The MCA-ELISA reached a sensitivity and specificity of 98% (CI = 94.7%-100%) and 63.6% (53.9-73.1), respectively. When anti-Trypanosoma cruzi reactive sera were excluded, the specificity reached 98.4% (94.4-100), while the sensitivity was similar, with a positive predictive value (PV) of 98.6% (94.6-100) and negative PV of 96.3% (91.6-100). The performance of the MCA-ELISA results strongly contribute to the final diagnostic decision, since a non-reactive serological result almost discards the suspected ATL, because of its high negative PV. The developed MCA-ELISA showed a high diagnostic performance, which makes it a good candidate for ATL diagnosis, for seroprevalence studies, or for monitoring treatments efficacy. |
| doi_str_mv | 10.1371/journal.pone.0232829 |
| format | Article |
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Leishmania (Viannia) braziliensis is the causal agent of the 90% of Argentinean ATL cases. Considering the current knowledge, an ELISA based crude antigen (CA) for the diagnosis was designed. Ninety-nine subjects diagnosed as ATL, 27 as no-ATL, and 84 donors from non-ATL-endemic areas were included in this study. The current ATL diagnosis was based four techniques, dermal smear microscopic examination (parasitological test), PCR, Leishmanin skin test, and clinical records. We obtained CA extracts from promastigotes and amastigotes from macrophage cultures of different zymodemes of endemic Leishmania species circulating in the study area. Crude antigens from the 'local' main zymodeme of L. (V.) braziliensis showed the highest reactivity against anti-Leishmania antibodies compared to the other included species. The CA of amastigotes of this zymodeme was 3.4 fold more reactive than promastigotes one. Moreover, amastigote-membrane CA (MCA) were 3.6 fold more reactive than the soluble antigens. The MCA-ELISA reached a sensitivity and specificity of 98% (CI = 94.7%-100%) and 63.6% (53.9-73.1), respectively. When anti-Trypanosoma cruzi reactive sera were excluded, the specificity reached 98.4% (94.4-100), while the sensitivity was similar, with a positive predictive value (PV) of 98.6% (94.6-100) and negative PV of 96.3% (91.6-100). The performance of the MCA-ELISA results strongly contribute to the final diagnostic decision, since a non-reactive serological result almost discards the suspected ATL, because of its high negative PV. The developed MCA-ELISA showed a high diagnostic performance, which makes it a good candidate for ATL diagnosis, for seroprevalence studies, or for monitoring treatments efficacy.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0232829</identifier><identifier>PMID: 32379842</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amastigotes ; Antibodies ; Antigenic determinants ; Antigens ; Biology and Life Sciences ; Diagnosis ; Diagnostic systems ; Endemic species ; Enzyme-linked immunosorbent assay ; Enzymes ; Laboratories ; Leishmania ; Leishmaniasis ; Leishmanin ; Macrophages ; Medical diagnosis ; Medical records ; Medical schools ; Medical tests ; Medicine and Health Sciences ; Membranes ; Methods ; Microscopy ; Molecular diagnostic techniques ; Parasite antigens ; Parasites ; Parasitic diseases ; Parasitology ; Promastigotes ; Research and Analysis Methods ; Sensitivity ; Serology ; Skin ; Skin tests ; Tegumentary leishmaniasis ; Testing ; Tropical diseases ; Vector-borne diseases</subject><ispartof>PloS one, 2020-05, Vol.15 (5), p.e0232829-e0232829</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Bracamonte et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Leishmania (Viannia) braziliensis is the causal agent of the 90% of Argentinean ATL cases. Considering the current knowledge, an ELISA based crude antigen (CA) for the diagnosis was designed. Ninety-nine subjects diagnosed as ATL, 27 as no-ATL, and 84 donors from non-ATL-endemic areas were included in this study. The current ATL diagnosis was based four techniques, dermal smear microscopic examination (parasitological test), PCR, Leishmanin skin test, and clinical records. We obtained CA extracts from promastigotes and amastigotes from macrophage cultures of different zymodemes of endemic Leishmania species circulating in the study area. Crude antigens from the 'local' main zymodeme of L. (V.) braziliensis showed the highest reactivity against anti-Leishmania antibodies compared to the other included species. The CA of amastigotes of this zymodeme was 3.4 fold more reactive than promastigotes one. Moreover, amastigote-membrane CA (MCA) were 3.6 fold more reactive than the soluble antigens. The MCA-ELISA reached a sensitivity and specificity of 98% (CI = 94.7%-100%) and 63.6% (53.9-73.1), respectively. When anti-Trypanosoma cruzi reactive sera were excluded, the specificity reached 98.4% (94.4-100), while the sensitivity was similar, with a positive predictive value (PV) of 98.6% (94.6-100) and negative PV of 96.3% (91.6-100). The performance of the MCA-ELISA results strongly contribute to the final diagnostic decision, since a non-reactive serological result almost discards the suspected ATL, because of its high negative PV. The developed MCA-ELISA showed a high diagnostic performance, which makes it a good candidate for ATL diagnosis, for seroprevalence studies, or for monitoring treatments efficacy.</description><subject>Amastigotes</subject><subject>Antibodies</subject><subject>Antigenic determinants</subject><subject>Antigens</subject><subject>Biology and Life Sciences</subject><subject>Diagnosis</subject><subject>Diagnostic systems</subject><subject>Endemic species</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzymes</subject><subject>Laboratories</subject><subject>Leishmania</subject><subject>Leishmaniasis</subject><subject>Leishmanin</subject><subject>Macrophages</subject><subject>Medical diagnosis</subject><subject>Medical records</subject><subject>Medical schools</subject><subject>Medical tests</subject><subject>Medicine and Health Sciences</subject><subject>Membranes</subject><subject>Methods</subject><subject>Microscopy</subject><subject>Molecular diagnostic techniques</subject><subject>Parasite antigens</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>Parasitology</subject><subject>Promastigotes</subject><subject>Research and Analysis Methods</subject><subject>Sensitivity</subject><subject>Serology</subject><subject>Skin</subject><subject>Skin tests</subject><subject>Tegumentary leishmaniasis</subject><subject>Testing</subject><subject>Tropical diseases</subject><subject>Vector-borne 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performance of an enzyme linked immunosorbent assay for American tegumentary leishmaniasis diagnosis with Leishmania (Viannia) braziliensis amastigotes membrane crude antigens</title><author>Bracamonte, María E ; Álvarez, Agustín Moya ; Sosa, Andrea M ; Hoyos, Carlos L ; Lauthier, Juan J ; Cajal, Silvana P ; Juarez, Marisa ; Uncos, Renato E ; Sánchez-Valdéz, Fernando J ; Acuña, Leonardo ; Diosque, Patricio ; Basombrío, Miguel A ; Nasser, Julio R ; Hashiguchi, Yoshihisa ; Korenaga, Masataka ; Barroso, Paola A ; Marco, Jorge D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c593t-a1fef0b213a47927b807bcc50bd2239d0ed55b35df0c93023dbf6bbc998ed7a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Amastigotes</topic><topic>Antibodies</topic><topic>Antigenic determinants</topic><topic>Antigens</topic><topic>Biology and Life 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Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bracamonte, María E</au><au>Álvarez, Agustín Moya</au><au>Sosa, Andrea M</au><au>Hoyos, Carlos L</au><au>Lauthier, Juan J</au><au>Cajal, Silvana P</au><au>Juarez, Marisa</au><au>Uncos, Renato E</au><au>Sánchez-Valdéz, Fernando J</au><au>Acuña, Leonardo</au><au>Diosque, Patricio</au><au>Basombrío, Miguel A</au><au>Nasser, Julio R</au><au>Hashiguchi, Yoshihisa</au><au>Korenaga, Masataka</au><au>Barroso, Paola A</au><au>Marco, Jorge D</au><au>Dikhit, Manas Ranjan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High performance of an enzyme linked immunosorbent assay for American tegumentary leishmaniasis diagnosis with Leishmania (Viannia) braziliensis amastigotes membrane crude antigens</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2020-05-07</date><risdate>2020</risdate><volume>15</volume><issue>5</issue><spage>e0232829</spage><epage>e0232829</epage><pages>e0232829-e0232829</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The diagnosis of American tegumentary leishmaniasis (ATL) still requires the design of more effective tools. Leishmania (Viannia) braziliensis is the causal agent of the 90% of Argentinean ATL cases. Considering the current knowledge, an ELISA based crude antigen (CA) for the diagnosis was designed. Ninety-nine subjects diagnosed as ATL, 27 as no-ATL, and 84 donors from non-ATL-endemic areas were included in this study. The current ATL diagnosis was based four techniques, dermal smear microscopic examination (parasitological test), PCR, Leishmanin skin test, and clinical records. We obtained CA extracts from promastigotes and amastigotes from macrophage cultures of different zymodemes of endemic Leishmania species circulating in the study area. Crude antigens from the 'local' main zymodeme of L. (V.) braziliensis showed the highest reactivity against anti-Leishmania antibodies compared to the other included species. The CA of amastigotes of this zymodeme was 3.4 fold more reactive than promastigotes one. Moreover, amastigote-membrane CA (MCA) were 3.6 fold more reactive than the soluble antigens. The MCA-ELISA reached a sensitivity and specificity of 98% (CI = 94.7%-100%) and 63.6% (53.9-73.1), respectively. When anti-Trypanosoma cruzi reactive sera were excluded, the specificity reached 98.4% (94.4-100), while the sensitivity was similar, with a positive predictive value (PV) of 98.6% (94.6-100) and negative PV of 96.3% (91.6-100). The performance of the MCA-ELISA results strongly contribute to the final diagnostic decision, since a non-reactive serological result almost discards the suspected ATL, because of its high negative PV. The developed MCA-ELISA showed a high diagnostic performance, which makes it a good candidate for ATL diagnosis, for seroprevalence studies, or for monitoring treatments efficacy.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32379842</pmid><doi>10.1371/journal.pone.0232829</doi><orcidid>https://orcid.org/0000-0002-9125-8124</orcidid><orcidid>https://orcid.org/0000-0002-4408-0941</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2020-05, Vol.15 (5), p.e0232829-e0232829 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2399837305 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Amastigotes Antibodies Antigenic determinants Antigens Biology and Life Sciences Diagnosis Diagnostic systems Endemic species Enzyme-linked immunosorbent assay Enzymes Laboratories Leishmania Leishmaniasis Leishmanin Macrophages Medical diagnosis Medical records Medical schools Medical tests Medicine and Health Sciences Membranes Methods Microscopy Molecular diagnostic techniques Parasite antigens Parasites Parasitic diseases Parasitology Promastigotes Research and Analysis Methods Sensitivity Serology Skin Skin tests Tegumentary leishmaniasis Testing Tropical diseases Vector-borne diseases |
| title | High performance of an enzyme linked immunosorbent assay for American tegumentary leishmaniasis diagnosis with Leishmania (Viannia) braziliensis amastigotes membrane crude antigens |
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