Effect of ovarian stimulation on the expression of piRNA pathway proteins

PIWI-interacting RNAs (piRNAs) play an important role in gametogenesis, fertility and embryonic development. The current study investigated the effect of different doses of pregnant mare serum gonadotrophin/human chorionic gonadotrophin (PMSG/hCG) and repeated ovarian stimulation (OS) on the express...

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Veröffentlicht in:	PloS one 2020-05, Vol.15 (5), p.e0232629-e0232629
Hauptverfasser:	Sari, Ismail, Gumus, Erkan, Taskiran, Ahmet Sevki, Karakoc Sokmensuer, Lale
Format:	Artikel
Sprache:	eng
Schlagworte:	Biology and Life Sciences Biosynthesis Deoxyribonucleic acid DNA DNA methylation EDTA Embryogenesis Embryology Embryonic development Embryonic growth stage Embryos Epigenetics Fertility Gametogenesis Gene expression Gene loci Genes Genomes Genomics Gonadotropins Histology In vitro fertilization Infertility Medicine Messenger RNA mRNA Operating systems (Software) Pituitary (anterior) Placental hormones Pregnant women Proteins Research and Analysis Methods RNA Silence Stimulation Table tennis Transposons
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Zusammenfassung:	PIWI-interacting RNAs (piRNAs) play an important role in gametogenesis, fertility and embryonic development. The current study investigated the effect of different doses of pregnant mare serum gonadotrophin/human chorionic gonadotrophin (PMSG/hCG) and repeated ovarian stimulation (OS) on the expression of the Mili, Miwi, Mael, Tdrd1, Tdrd9, qnd Mitopld genes, which have crucial roles in the biogenesis and function of piRNAs. Here, we found that after treatment with 7.5 I.U. PMSG/hCG and two repeated rounds of OS, both the mRNA and protein levels of Tdrd9, Tdrd1 and Mael showed the greatest decrease in the ovarian tissue, but the plasma E2 levels showed the strongest increases (p
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0232629