How exogenous nitric oxide regulates nitrogen assimilation in wheat seedlings under different nitrogen sources and levels
Nitrogen (N) is one of the most important nutrients for plants and nitric oxide (NO) as a signaling plant growth regulator involved in nitrogen assimilation. Understanding the influence of exogenous NO on nitrogen metabolism at the gene expression and enzyme activity levels under different sources o...
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| creator | Balotf, Sadegh Islam, Shahidul Kavoosi, Gholamreza Kholdebarin, Bahman Juhasz, Angela Ma, Wujun |
| description | Nitrogen (N) is one of the most important nutrients for plants and nitric oxide (NO) as a signaling plant growth regulator involved in nitrogen assimilation. Understanding the influence of exogenous NO on nitrogen metabolism at the gene expression and enzyme activity levels under different sources of nitrogen is vitally important for increasing nitrogen use efficiency (NUE). This study investigated the expression of key genes and enzymes in relation to nitrogen assimilation in two Australian wheat cultivars, a popular high NUE cv. Spitfire and a normal NUE cv. Westonia, under different combinations of nitrogen and sodium nitroprusside (SNP) as the NO donor. Application of NO increased the gene expressions and activities of nitrogen assimilation pathway enzymes in both cultivars at low levels of nitrogen. At high nitrogen supplies, the expressions and activities of N assimilation genes increased in response to exogenous NO only in cv. Spitfire but not in cv. Westonia. Exogenous NO caused an increase in leaf NO content at low N supplies in both cultivars, while under high nitrogen treatments, cv. Spitfire showed an increase under ammonium nitrate (NH4NO3) treatment but cv. Westonia was not affected. N assimilation gene expression and enzyme activity showed a clear relationship between exogenous NO, N concentration and N forms in primary plant nitrogen assimilation. Results reveal the possible role of NO and different nitrogen sources on nitrogen assimilation in Triticum aestivum plants. |
| doi_str_mv | 10.1371/journal.pone.0190269 |
| format | Article |
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Understanding the influence of exogenous NO on nitrogen metabolism at the gene expression and enzyme activity levels under different sources of nitrogen is vitally important for increasing nitrogen use efficiency (NUE). This study investigated the expression of key genes and enzymes in relation to nitrogen assimilation in two Australian wheat cultivars, a popular high NUE cv. Spitfire and a normal NUE cv. Westonia, under different combinations of nitrogen and sodium nitroprusside (SNP) as the NO donor. Application of NO increased the gene expressions and activities of nitrogen assimilation pathway enzymes in both cultivars at low levels of nitrogen. At high nitrogen supplies, the expressions and activities of N assimilation genes increased in response to exogenous NO only in cv. Spitfire but not in cv. Westonia. Exogenous NO caused an increase in leaf NO content at low N supplies in both cultivars, while under high nitrogen treatments, cv. Spitfire showed an increase under ammonium nitrate (NH4NO3) treatment but cv. Westonia was not affected. N assimilation gene expression and enzyme activity showed a clear relationship between exogenous NO, N concentration and N forms in primary plant nitrogen assimilation. Results reveal the possible role of NO and different nitrogen sources on nitrogen assimilation in Triticum aestivum plants.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0190269</identifier><identifier>PMID: 29320529</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agricultural production ; Ammonium ; Ammonium nitrate ; Assimilation ; Australia ; Biological assimilation ; Biology and Life Sciences ; Crops ; Cultivars ; Efficiency ; Enzymatic activity ; Enzyme activity ; Enzymes ; Gene expression ; Gene Expression Regulation, Plant ; Genes ; Genes, Plant ; Glutamate-Ammonia Ligase - metabolism ; Glycerol ; Growth regulators ; Health aspects ; Levels ; Life sciences ; Metabolism ; Nitrate Reductase - metabolism ; Nitrates ; Nitric oxide ; Nitric Oxide - pharmacology ; Nitrogen ; Nitrogen - metabolism ; Nitrogen sources ; Nutrients ; Physical Sciences ; Physiology ; Plant growth ; Plant Leaves - metabolism ; Plants (Organisms) ; Polymorphism, Single Nucleotide ; Seedlings ; Seedlings - drug effects ; Seedlings - metabolism ; Single-nucleotide polymorphism ; Sodium nitroprusside ; Triticum - enzymology ; Triticum - genetics ; Triticum - growth & development ; Triticum aestivum ; Wheat</subject><ispartof>PloS one, 2018-01, Vol.13 (1), p.e0190269-e0190269</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Balotf et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Balotf et al 2018 Balotf et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-59646b45dec8c85df55f721c055511301c18d076c871c90592bac87ef0b9b573</citedby><cites>FETCH-LOGICAL-c692t-59646b45dec8c85df55f721c055511301c18d076c871c90592bac87ef0b9b573</cites><orcidid>0000-0002-1264-866X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761883/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761883/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29320529$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Balotf, Sadegh</creatorcontrib><creatorcontrib>Islam, Shahidul</creatorcontrib><creatorcontrib>Kavoosi, Gholamreza</creatorcontrib><creatorcontrib>Kholdebarin, Bahman</creatorcontrib><creatorcontrib>Juhasz, Angela</creatorcontrib><creatorcontrib>Ma, Wujun</creatorcontrib><title>How exogenous nitric oxide regulates nitrogen assimilation in wheat seedlings under different nitrogen sources and levels</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Nitrogen (N) is one of the most important nutrients for plants and nitric oxide (NO) as a signaling plant growth regulator involved in nitrogen assimilation. Understanding the influence of exogenous NO on nitrogen metabolism at the gene expression and enzyme activity levels under different sources of nitrogen is vitally important for increasing nitrogen use efficiency (NUE). This study investigated the expression of key genes and enzymes in relation to nitrogen assimilation in two Australian wheat cultivars, a popular high NUE cv. Spitfire and a normal NUE cv. Westonia, under different combinations of nitrogen and sodium nitroprusside (SNP) as the NO donor. Application of NO increased the gene expressions and activities of nitrogen assimilation pathway enzymes in both cultivars at low levels of nitrogen. At high nitrogen supplies, the expressions and activities of N assimilation genes increased in response to exogenous NO only in cv. Spitfire but not in cv. Westonia. Exogenous NO caused an increase in leaf NO content at low N supplies in both cultivars, while under high nitrogen treatments, cv. Spitfire showed an increase under ammonium nitrate (NH4NO3) treatment but cv. Westonia was not affected. N assimilation gene expression and enzyme activity showed a clear relationship between exogenous NO, N concentration and N forms in primary plant nitrogen assimilation. Results reveal the possible role of NO and different nitrogen sources on nitrogen assimilation in Triticum aestivum plants.</description><subject>Agricultural production</subject><subject>Ammonium</subject><subject>Ammonium nitrate</subject><subject>Assimilation</subject><subject>Australia</subject><subject>Biological assimilation</subject><subject>Biology and Life Sciences</subject><subject>Crops</subject><subject>Cultivars</subject><subject>Efficiency</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Enzymes</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genes, Plant</subject><subject>Glutamate-Ammonia Ligase - metabolism</subject><subject>Glycerol</subject><subject>Growth regulators</subject><subject>Health aspects</subject><subject>Levels</subject><subject>Life sciences</subject><subject>Metabolism</subject><subject>Nitrate Reductase - metabolism</subject><subject>Nitrates</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - pharmacology</subject><subject>Nitrogen</subject><subject>Nitrogen - metabolism</subject><subject>Nitrogen sources</subject><subject>Nutrients</subject><subject>Physical Sciences</subject><subject>Physiology</subject><subject>Plant growth</subject><subject>Plant Leaves - metabolism</subject><subject>Plants (Organisms)</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Seedlings</subject><subject>Seedlings - drug effects</subject><subject>Seedlings - metabolism</subject><subject>Single-nucleotide polymorphism</subject><subject>Sodium nitroprusside</subject><subject>Triticum - enzymology</subject><subject>Triticum - genetics</subject><subject>Triticum - growth & development</subject><subject>Triticum aestivum</subject><subject>Wheat</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk01v1DAQhiMEoqXwDxBEQkJw2MV2Yie-IFUV0JUqVYKKq-U4k6xXXnuxk378e2bZtOyiHlAOmYyfeR2_nsmy15TMaVHRT6swRq_dfBM8zAmVhAn5JDumsmAzwUjxdC8-yl6ktCKEF7UQz7MjhguEM3mc3Z2HmxxuQw8-jCn3dojW5OHWtpBH6EenB9ilt0iuU7Jri0kbfG59frMEPeQJoHXW9ykffQsxb23XQQQ__C1M-LcGlbRvcwfX4NLL7FmnXYJX0_sku_r65ersfHZx-W1xdnoxM0KyYcalKEVT8hZMbWredpx3FaOGcM4pLQg1tG5JJUxdUSMJl6zRGENHGtnwqjjJ3u5kNy4kNZmWFCskEUygaUgsdkQb9Eptol3reKeCtupPIsRe6ThY40AxUVaNEQz1y7I0ssbQ8AaANvjVcNT6PO02NmtoDXoQtTsQPVzxdqn6cK14JWhdFyjwYRKI4dcIaVBrmww4pz3gBSkqa8kFZyVB9N0_6OOnm6he4wGs7wLua7ai6pQzxmlRlBSp-SMUPi2srcEO6yzmDwo-HhQgM8Dt0OsxJbX48f3_2cufh-z7PRa7yw3LFNy4bbh0CJY70MSQUoTuwWRK1HZA7t1Q2wFR04Bg2Zv9C3ooup-I4jfDiQxZ</recordid><startdate>20180110</startdate><enddate>20180110</enddate><creator>Balotf, Sadegh</creator><creator>Islam, Shahidul</creator><creator>Kavoosi, Gholamreza</creator><creator>Kholdebarin, Bahman</creator><creator>Juhasz, Angela</creator><creator>Ma, Wujun</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-1264-866X</orcidid></search><sort><creationdate>20180110</creationdate><title>How exogenous nitric oxide regulates nitrogen assimilation in wheat seedlings under different nitrogen sources and levels</title><author>Balotf, Sadegh ; Islam, Shahidul ; Kavoosi, Gholamreza ; Kholdebarin, Bahman ; Juhasz, Angela ; Ma, Wujun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-59646b45dec8c85df55f721c055511301c18d076c871c90592bac87ef0b9b573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Agricultural production</topic><topic>Ammonium</topic><topic>Ammonium nitrate</topic><topic>Assimilation</topic><topic>Australia</topic><topic>Biological assimilation</topic><topic>Biology and Life Sciences</topic><topic>Crops</topic><topic>Cultivars</topic><topic>Efficiency</topic><topic>Enzymatic activity</topic><topic>Enzyme activity</topic><topic>Enzymes</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Genes, Plant</topic><topic>Glutamate-Ammonia Ligase - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Balotf, Sadegh</au><au>Islam, Shahidul</au><au>Kavoosi, Gholamreza</au><au>Kholdebarin, Bahman</au><au>Juhasz, Angela</au><au>Ma, Wujun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>How exogenous nitric oxide regulates nitrogen assimilation in wheat seedlings under different nitrogen sources and levels</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-01-10</date><risdate>2018</risdate><volume>13</volume><issue>1</issue><spage>e0190269</spage><epage>e0190269</epage><pages>e0190269-e0190269</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Nitrogen (N) is one of the most important nutrients for plants and nitric oxide (NO) as a signaling plant growth regulator involved in nitrogen assimilation. Understanding the influence of exogenous NO on nitrogen metabolism at the gene expression and enzyme activity levels under different sources of nitrogen is vitally important for increasing nitrogen use efficiency (NUE). This study investigated the expression of key genes and enzymes in relation to nitrogen assimilation in two Australian wheat cultivars, a popular high NUE cv. Spitfire and a normal NUE cv. Westonia, under different combinations of nitrogen and sodium nitroprusside (SNP) as the NO donor. Application of NO increased the gene expressions and activities of nitrogen assimilation pathway enzymes in both cultivars at low levels of nitrogen. At high nitrogen supplies, the expressions and activities of N assimilation genes increased in response to exogenous NO only in cv. Spitfire but not in cv. Westonia. Exogenous NO caused an increase in leaf NO content at low N supplies in both cultivars, while under high nitrogen treatments, cv. Spitfire showed an increase under ammonium nitrate (NH4NO3) treatment but cv. Westonia was not affected. N assimilation gene expression and enzyme activity showed a clear relationship between exogenous NO, N concentration and N forms in primary plant nitrogen assimilation. Results reveal the possible role of NO and different nitrogen sources on nitrogen assimilation in Triticum aestivum plants.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29320529</pmid><doi>10.1371/journal.pone.0190269</doi><tpages>e0190269</tpages><orcidid>https://orcid.org/0000-0002-1264-866X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Agricultural production Ammonium Ammonium nitrate Assimilation Australia Biological assimilation Biology and Life Sciences Crops Cultivars Efficiency Enzymatic activity Enzyme activity Enzymes Gene expression Gene Expression Regulation, Plant Genes Genes, Plant Glutamate-Ammonia Ligase - metabolism Glycerol Growth regulators Health aspects Levels Life sciences Metabolism Nitrate Reductase - metabolism Nitrates Nitric oxide Nitric Oxide - pharmacology Nitrogen Nitrogen - metabolism Nitrogen sources Nutrients Physical Sciences Physiology Plant growth Plant Leaves - metabolism Plants (Organisms) Polymorphism, Single Nucleotide Seedlings Seedlings - drug effects Seedlings - metabolism Single-nucleotide polymorphism Sodium nitroprusside Triticum - enzymology Triticum - genetics Triticum - growth & development Triticum aestivum Wheat |
| title | How exogenous nitric oxide regulates nitrogen assimilation in wheat seedlings under different nitrogen sources and levels |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T16%3A49%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=How%20exogenous%20nitric%20oxide%20regulates%20nitrogen%20assimilation%20in%20wheat%20seedlings%20under%20different%20nitrogen%20sources%20and%20levels&rft.jtitle=PloS%20one&rft.au=Balotf,%20Sadegh&rft.date=2018-01-10&rft.volume=13&rft.issue=1&rft.spage=e0190269&rft.epage=e0190269&rft.pages=e0190269-e0190269&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0190269&rft_dat=%3Cgale_plos_%3EA522513341%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2390626026&rft_id=info:pmid/29320529&rft_galeid=A522513341&rft_doaj_id=oai_doaj_org_article_2647bc6287e444c98628c5bee1b4c9b5&rfr_iscdi=true |