Wine yeast phenomics: A standardized fermentation method for assessing quantitative traits of Saccharomyces cerevisiae strains in enological conditions
This work describes the set up of a small scale fermentation methodology for measuring quantitative traits of hundreds of samples in an enological context. By using standardized screw cap vessels, the alcoholic fermentation kinetics of Saccharomyces cerevisiae strains were measured by following thei...
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| Veröffentlicht in: | PloS one 2018-01, Vol.13 (1), p.e0190094-e0190094 |
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| creator | Peltier, Emilien Bernard, Margaux Trujillo, Marine Prodhomme, Duyên Barbe, Jean-Christophe Gibon, Yves Marullo, Philippe |
| description | This work describes the set up of a small scale fermentation methodology for measuring quantitative traits of hundreds of samples in an enological context. By using standardized screw cap vessels, the alcoholic fermentation kinetics of Saccharomyces cerevisiae strains were measured by following their weight loss over the time. This dispositive was coupled with robotized enzymatic assays for measuring metabolites of enological interest in natural grape juices. Despite the small volume used, kinetic parameters and fermentation end products measured are similar with those observed in larger scale vats. The vessel used also offers the possibility to assay 32 volatiles compounds using a headspace solid-phase micro-extraction coupled to gas chromatography and mass spectrometry. The vessel shaking applied strongly impacted most of the phenotypes investigated due to oxygen transfer occuring in the first hours of the alcoholic fermentation. The impact of grape must and micro-oxygenation was investigated illustrating some relevant genetic x environmental interactions. By phenotyping a wide panel of commercial wine starters in five grape juices, broad phenotypic correlations between kinetics and metabolic end products were evidentiated. Moreover, a multivariate analysis illustrates that some grape musts are more able than others to discriminate commercial strains since some are less robust to environmental changes. |
| doi_str_mv | 10.1371/journal.pone.0190094 |
| format | Article |
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By using standardized screw cap vessels, the alcoholic fermentation kinetics of Saccharomyces cerevisiae strains were measured by following their weight loss over the time. This dispositive was coupled with robotized enzymatic assays for measuring metabolites of enological interest in natural grape juices. Despite the small volume used, kinetic parameters and fermentation end products measured are similar with those observed in larger scale vats. The vessel used also offers the possibility to assay 32 volatiles compounds using a headspace solid-phase micro-extraction coupled to gas chromatography and mass spectrometry. The vessel shaking applied strongly impacted most of the phenotypes investigated due to oxygen transfer occuring in the first hours of the alcoholic fermentation. The impact of grape must and micro-oxygenation was investigated illustrating some relevant genetic x environmental interactions. 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By using standardized screw cap vessels, the alcoholic fermentation kinetics of Saccharomyces cerevisiae strains were measured by following their weight loss over the time. This dispositive was coupled with robotized enzymatic assays for measuring metabolites of enological interest in natural grape juices. Despite the small volume used, kinetic parameters and fermentation end products measured are similar with those observed in larger scale vats. The vessel used also offers the possibility to assay 32 volatiles compounds using a headspace solid-phase micro-extraction coupled to gas chromatography and mass spectrometry. The vessel shaking applied strongly impacted most of the phenotypes investigated due to oxygen transfer occuring in the first hours of the alcoholic fermentation. The impact of grape must and micro-oxygenation was investigated illustrating some relevant genetic x environmental interactions. 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yeast phenomics: A standardized fermentation method for assessing quantitative traits of Saccharomyces cerevisiae strains in enological conditions</title><author>Peltier, Emilien ; Bernard, Margaux ; Trujillo, Marine ; Prodhomme, Duyên ; Barbe, Jean-Christophe ; Gibon, Yves ; Marullo, Philippe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c726t-344cf790b9ef0ee3365a4936a7a18ba3eebdca36c3246619afef00a9dae5372f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Biology and Life Sciences</topic><topic>Cellulose acetate</topic><topic>Chemical and Process Engineering</topic><topic>Engineering Sciences</topic><topic>Environmental changes</topic><topic>Environmental Sciences</topic><topic>Fermentation</topic><topic>Food engineering</topic><topic>Gas chromatography</topic><topic>Genetic 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describes the set up of a small scale fermentation methodology for measuring quantitative traits of hundreds of samples in an enological context. By using standardized screw cap vessels, the alcoholic fermentation kinetics of Saccharomyces cerevisiae strains were measured by following their weight loss over the time. This dispositive was coupled with robotized enzymatic assays for measuring metabolites of enological interest in natural grape juices. Despite the small volume used, kinetic parameters and fermentation end products measured are similar with those observed in larger scale vats. The vessel used also offers the possibility to assay 32 volatiles compounds using a headspace solid-phase micro-extraction coupled to gas chromatography and mass spectrometry. The vessel shaking applied strongly impacted most of the phenotypes investigated due to oxygen transfer occuring in the first hours of the alcoholic fermentation. The impact of grape must and micro-oxygenation was investigated illustrating some relevant genetic x environmental interactions. 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| subjects | Biology and Life Sciences Cellulose acetate Chemical and Process Engineering Engineering Sciences Environmental changes Environmental Sciences Fermentation Food engineering Gas chromatography Genetic aspects Genomes Glycerol Grapes Headspace Kinetics Laboratories Life Sciences Mass spectrometry Mass spectroscopy Medicine and Health Sciences Metabolism Metabolites Methods Multivariate analysis Musts Oxygen Oxygen transfer Oxygenation Phenotypes Phenotyping Physical Sciences Physiological aspects Quantitative genetics Research and Analysis Methods Saccharomyces cerevisiae Shaking Solid phases Starters Sugar Vegetal Biology Vessels Volatile compounds Volatiles Weight loss measurement Wine Wine microbiology Wines Yeast Yeasts |
| title | Wine yeast phenomics: A standardized fermentation method for assessing quantitative traits of Saccharomyces cerevisiae strains in enological conditions |
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