Prevalence and pollution characteristics of antibiotic resistant genes in one high anthropogenically-impacted river
The objectives of this study were to comprehensively investigate the occurrence, distribution, and mobility of antibiotic resistant genes (ARGs) in the biofilm, water, and sediment from a section of the Weihe-river, in the northern Henan province, China. The abundances of nine ARGs belonging to four...
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| Veröffentlicht in: | PloS one 2020-04, Vol.15 (4), p.e0231128-e0231128 |
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| description | The objectives of this study were to comprehensively investigate the occurrence, distribution, and mobility of antibiotic resistant genes (ARGs) in the biofilm, water, and sediment from a section of the Weihe-river, in the northern Henan province, China. The abundances of nine ARGs belonging to four commonly used antibiotic classes (tetracyclines, sulfonamides, fluoroquinolones, and multidrug) and class 1 integron-integrase gene (intI1) were quantified. Sulfonamides gene (sulI) accounted for the highest percentage of detected ARGs in most sampling sites, including in water, biofilm, and sediment. Among the resistance genes, IntI1 and sul1 were significantly correlated (r>0.800, p |
| doi_str_mv | 10.1371/journal.pone.0231128 |
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The abundances of nine ARGs belonging to four commonly used antibiotic classes (tetracyclines, sulfonamides, fluoroquinolones, and multidrug) and class 1 integron-integrase gene (intI1) were quantified. Sulfonamides gene (sulI) accounted for the highest percentage of detected ARGs in most sampling sites, including in water, biofilm, and sediment. Among the resistance genes, IntI1 and sul1 were significantly correlated (r>0.800, p<0.01) with a fecal coliform (FC) detected in the biofilm, and there was also a significantly positive correlation between the abundances of 16SrRNA and intI1 in the biofilms. Compared with the sediment and water samples, the biofilms contained sufficient nutrients to promote bacterial reproduction. Under sufficient total nitrogen and phosphorus concentrations, the horizontal gene transfer due to intI1 plays a key role in the formation and migration of ARGs within biofilms.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0231128</identifier><identifier>PMID: 32271821</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abundance ; Amides ; Anthropogenic factors ; Antibiotic resistance ; Antibiotics ; Bacteria ; Biofilms ; Biology and Life Sciences ; Deoxyribonucleic acid ; DNA ; Earth Sciences ; Ecology and Environmental Sciences ; Ecosystems ; Endangered & extinct species ; Fluoroquinolones ; Gene transfer ; Genes ; Genetic testing ; Horizontal transfer ; Integrase ; Laboratories ; Medicine and Health Sciences ; Microbial drug resistance ; Microorganisms ; Nutrients ; Phosphorus ; Pollutants ; Pollution ; Potassium ; River ecology ; Rivers ; Sediments ; Sediments (Geology) ; Studies ; Sulfonamides ; Tetracyclines ; Water ; Water analysis ; Water pollution ; Water sampling ; Watershed management ; Watersheds</subject><ispartof>PloS one, 2020-04, Vol.15 (4), p.e0231128-e0231128</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Li, Zhang. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Li, Zhang 2020 Li, Zhang</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c743t-df75e37613b4244a522df487fbe79f85ef75cae518cd973a21babff5b45065a53</citedby><cites>FETCH-LOGICAL-c743t-df75e37613b4244a522df487fbe79f85ef75cae518cd973a21babff5b45065a53</cites><orcidid>0000-0003-1383-7160</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7145097/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7145097/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32271821$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Zhao, Hui</contributor><creatorcontrib>Li, Qingzhao</creatorcontrib><creatorcontrib>Zhang, Qiuling</creatorcontrib><title>Prevalence and pollution characteristics of antibiotic resistant genes in one high anthropogenically-impacted river</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The objectives of this study were to comprehensively investigate the occurrence, distribution, and mobility of antibiotic resistant genes (ARGs) in the biofilm, water, and sediment from a section of the Weihe-river, in the northern Henan province, China. The abundances of nine ARGs belonging to four commonly used antibiotic classes (tetracyclines, sulfonamides, fluoroquinolones, and multidrug) and class 1 integron-integrase gene (intI1) were quantified. Sulfonamides gene (sulI) accounted for the highest percentage of detected ARGs in most sampling sites, including in water, biofilm, and sediment. Among the resistance genes, IntI1 and sul1 were significantly correlated (r>0.800, p<0.01) with a fecal coliform (FC) detected in the biofilm, and there was also a significantly positive correlation between the abundances of 16SrRNA and intI1 in the biofilms. Compared with the sediment and water samples, the biofilms contained sufficient nutrients to promote bacterial reproduction. Under sufficient total nitrogen and phosphorus concentrations, the horizontal gene transfer due to intI1 plays a key role in the formation and migration of ARGs within biofilms.</description><subject>Abundance</subject><subject>Amides</subject><subject>Anthropogenic factors</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Biofilms</subject><subject>Biology and Life Sciences</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Earth Sciences</subject><subject>Ecology and Environmental Sciences</subject><subject>Ecosystems</subject><subject>Endangered & extinct species</subject><subject>Fluoroquinolones</subject><subject>Gene transfer</subject><subject>Genes</subject><subject>Genetic testing</subject><subject>Horizontal transfer</subject><subject>Integrase</subject><subject>Laboratories</subject><subject>Medicine and Health Sciences</subject><subject>Microbial drug resistance</subject><subject>Microorganisms</subject><subject>Nutrients</subject><subject>Phosphorus</subject><subject>Pollutants</subject><subject>Pollution</subject><subject>Potassium</subject><subject>River ecology</subject><subject>Rivers</subject><subject>Sediments</subject><subject>Sediments (Geology)</subject><subject>Studies</subject><subject>Sulfonamides</subject><subject>Tetracyclines</subject><subject>Water</subject><subject>Water analysis</subject><subject>Water pollution</subject><subject>Water sampling</subject><subject>Watershed 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One</addtitle><date>2020-04-09</date><risdate>2020</risdate><volume>15</volume><issue>4</issue><spage>e0231128</spage><epage>e0231128</epage><pages>e0231128-e0231128</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The objectives of this study were to comprehensively investigate the occurrence, distribution, and mobility of antibiotic resistant genes (ARGs) in the biofilm, water, and sediment from a section of the Weihe-river, in the northern Henan province, China. The abundances of nine ARGs belonging to four commonly used antibiotic classes (tetracyclines, sulfonamides, fluoroquinolones, and multidrug) and class 1 integron-integrase gene (intI1) were quantified. Sulfonamides gene (sulI) accounted for the highest percentage of detected ARGs in most sampling sites, including in water, biofilm, and sediment. Among the resistance genes, IntI1 and sul1 were significantly correlated (r>0.800, p<0.01) with a fecal coliform (FC) detected in the biofilm, and there was also a significantly positive correlation between the abundances of 16SrRNA and intI1 in the biofilms. Compared with the sediment and water samples, the biofilms contained sufficient nutrients to promote bacterial reproduction. Under sufficient total nitrogen and phosphorus concentrations, the horizontal gene transfer due to intI1 plays a key role in the formation and migration of ARGs within biofilms.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32271821</pmid><doi>10.1371/journal.pone.0231128</doi><tpages>e0231128</tpages><orcidid>https://orcid.org/0000-0003-1383-7160</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Abundance Amides Anthropogenic factors Antibiotic resistance Antibiotics Bacteria Biofilms Biology and Life Sciences Deoxyribonucleic acid DNA Earth Sciences Ecology and Environmental Sciences Ecosystems Endangered & extinct species Fluoroquinolones Gene transfer Genes Genetic testing Horizontal transfer Integrase Laboratories Medicine and Health Sciences Microbial drug resistance Microorganisms Nutrients Phosphorus Pollutants Pollution Potassium River ecology Rivers Sediments Sediments (Geology) Studies Sulfonamides Tetracyclines Water Water analysis Water pollution Water sampling Watershed management Watersheds |
| title | Prevalence and pollution characteristics of antibiotic resistant genes in one high anthropogenically-impacted river |
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