A high infectious simian adenovirus type 23 vector based vaccine efficiently protects common marmosets against Zika virus infection
Zika virus (ZIKV) has spread in many countries or territories causing severe neurologic complications with potential fatal outcomes. The small primate common marmosets are susceptible to ZIKV, mimicking key features of human infection. Here, a novel simian adenovirus type 23 vector-based vaccine exp...
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| Veröffentlicht in: | PLoS neglected tropical diseases 2020-02, Vol.14 (2), p.e0008027-e0008027 |
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| creator | Luo, Shengxue Zhao, Wei Ma, Xiaorui Zhang, Panli Liu, Bochao Zhang, Ling Wang, Wenjing Wang, Yuanzhan Fu, Yongshui Allain, Jean-Pierre Li, Tingting Li, Chengyao |
| description | Zika virus (ZIKV) has spread in many countries or territories causing severe neurologic complications with potential fatal outcomes. The small primate common marmosets are susceptible to ZIKV, mimicking key features of human infection. Here, a novel simian adenovirus type 23 vector-based vaccine expressing ZIKV pre-membrane-envelope proteins (Sad23L-prM-E) was produced in high infectious titer. Due to determination of immunogenicity in mice, a single-dose of 3×108 PFU Sad23L-prM-E vaccine was intramuscularly inoculated to marmosets. This vaccine raised antibody titers of 104.07 E-specific and 103.13 neutralizing antibody (NAb), as well as robust specific IFN-γ secreting T-cell response (1,219 SFCs/106 cells) to E peptides. The vaccinated marmosets, upon challenge with a high dose of ZIKV (105 PFU) six weeks post prime immunization, reduced viremia by more than 100 folds, and the low level of detectable viral RNA (103.66) and T-cell response (>726 SFCs/106 PBMCs) were acquired 1-2 weeks post exposure to ZIKV, while non-vaccinated control marmosets developed long-term high titer of ZIKV (105.73 copies/ml) (P |
| doi_str_mv | 10.1371/journal.pntd.0008027 |
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The small primate common marmosets are susceptible to ZIKV, mimicking key features of human infection. Here, a novel simian adenovirus type 23 vector-based vaccine expressing ZIKV pre-membrane-envelope proteins (Sad23L-prM-E) was produced in high infectious titer. Due to determination of immunogenicity in mice, a single-dose of 3×108 PFU Sad23L-prM-E vaccine was intramuscularly inoculated to marmosets. This vaccine raised antibody titers of 104.07 E-specific and 103.13 neutralizing antibody (NAb), as well as robust specific IFN-γ secreting T-cell response (1,219 SFCs/106 cells) to E peptides. The vaccinated marmosets, upon challenge with a high dose of ZIKV (105 PFU) six weeks post prime immunization, reduced viremia by more than 100 folds, and the low level of detectable viral RNA (<103 copies/ml) in blood, saliva, urine and feces was promptly eliminated when the secondary NAb (titer >103.66) and T-cell response (>726 SFCs/106 PBMCs) were acquired 1-2 weeks post exposure to ZIKV, while non-vaccinated control marmosets developed long-term high titer of ZIKV (105.73 copies/ml) (P<0.05). No significant pathological lesions were observed in marmoset tissues. Sad23L-prM-E vaccine was detectable in spleen, liver and PBMCs at least 4 months post challenge. In conclusion, a prime immunization with Sad23L-prM-E vaccine was able to protect marmosets against ZIKV infection when exposed to a high dose of ZIKV. This Sad23L-prM-E vaccine is a promising vaccine candidate for prevention of ZIKV infection in humans.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0008027</identifier><identifier>PMID: 32049958</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adenoviridae Infections - immunology ; Adenoviridae Infections - veterinary ; Adenoviridae Infections - virology ; Adenoviruses ; Adenoviruses, Simian - classification ; Animals ; Antibodies ; Biology and life sciences ; Biopharmaceuticals ; Biotechnology ; Callithrix ; Complications ; Disease control ; Immunization ; Immunogenicity ; Infection ; Infections ; Inoculation ; Laboratories ; Lesions ; Low level ; Lymphocytes T ; Medicine ; Medicine and Health Sciences ; Mimicry ; Monkey Diseases - immunology ; Monkey Diseases - virology ; Neurological complications ; Novels ; Nucleic acids ; Peptides ; Primates ; Proteins ; Public health ; Research and Analysis Methods ; Ribonucleic acid ; RNA ; Saliva ; Spleen ; T cells ; Tropical diseases ; Vaccination ; Vaccines ; Vector-borne diseases ; Viremia ; Viruses ; Zika virus ; Zika Virus Infection - immunology ; Zika Virus Infection - veterinary ; γ-Interferon</subject><ispartof>PLoS neglected tropical diseases, 2020-02, Vol.14 (2), p.e0008027-e0008027</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Luo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Luo et al 2020 Luo et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c624t-e34da508803446f81367a3c26ddb26e2bb0394bca6ba1d1355d6396cca546053</citedby><cites>FETCH-LOGICAL-c624t-e34da508803446f81367a3c26ddb26e2bb0394bca6ba1d1355d6396cca546053</cites><orcidid>0000-0001-5727-2179</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7015313/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7015313/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23868,27926,27927,53793,53795</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32049958$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Beasley, David W.C.</contributor><creatorcontrib>Luo, Shengxue</creatorcontrib><creatorcontrib>Zhao, Wei</creatorcontrib><creatorcontrib>Ma, Xiaorui</creatorcontrib><creatorcontrib>Zhang, Panli</creatorcontrib><creatorcontrib>Liu, Bochao</creatorcontrib><creatorcontrib>Zhang, Ling</creatorcontrib><creatorcontrib>Wang, Wenjing</creatorcontrib><creatorcontrib>Wang, Yuanzhan</creatorcontrib><creatorcontrib>Fu, Yongshui</creatorcontrib><creatorcontrib>Allain, Jean-Pierre</creatorcontrib><creatorcontrib>Li, Tingting</creatorcontrib><creatorcontrib>Li, Chengyao</creatorcontrib><title>A high infectious simian adenovirus type 23 vector based vaccine efficiently protects common marmosets against Zika virus infection</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>Zika virus (ZIKV) has spread in many countries or territories causing severe neurologic complications with potential fatal outcomes. The small primate common marmosets are susceptible to ZIKV, mimicking key features of human infection. Here, a novel simian adenovirus type 23 vector-based vaccine expressing ZIKV pre-membrane-envelope proteins (Sad23L-prM-E) was produced in high infectious titer. Due to determination of immunogenicity in mice, a single-dose of 3×108 PFU Sad23L-prM-E vaccine was intramuscularly inoculated to marmosets. This vaccine raised antibody titers of 104.07 E-specific and 103.13 neutralizing antibody (NAb), as well as robust specific IFN-γ secreting T-cell response (1,219 SFCs/106 cells) to E peptides. The vaccinated marmosets, upon challenge with a high dose of ZIKV (105 PFU) six weeks post prime immunization, reduced viremia by more than 100 folds, and the low level of detectable viral RNA (<103 copies/ml) in blood, saliva, urine and feces was promptly eliminated when the secondary NAb (titer >103.66) and T-cell response (>726 SFCs/106 PBMCs) were acquired 1-2 weeks post exposure to ZIKV, while non-vaccinated control marmosets developed long-term high titer of ZIKV (105.73 copies/ml) (P<0.05). No significant pathological lesions were observed in marmoset tissues. Sad23L-prM-E vaccine was detectable in spleen, liver and PBMCs at least 4 months post challenge. In conclusion, a prime immunization with Sad23L-prM-E vaccine was able to protect marmosets against ZIKV infection when exposed to a high dose of ZIKV. This Sad23L-prM-E vaccine is a promising vaccine candidate for prevention of ZIKV infection in humans.</description><subject>Adenoviridae Infections - immunology</subject><subject>Adenoviridae Infections - veterinary</subject><subject>Adenoviridae Infections - virology</subject><subject>Adenoviruses</subject><subject>Adenoviruses, Simian - classification</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Biology and life sciences</subject><subject>Biopharmaceuticals</subject><subject>Biotechnology</subject><subject>Callithrix</subject><subject>Complications</subject><subject>Disease control</subject><subject>Immunization</subject><subject>Immunogenicity</subject><subject>Infection</subject><subject>Infections</subject><subject>Inoculation</subject><subject>Laboratories</subject><subject>Lesions</subject><subject>Low level</subject><subject>Lymphocytes T</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Mimicry</subject><subject>Monkey Diseases - immunology</subject><subject>Monkey Diseases - virology</subject><subject>Neurological complications</subject><subject>Novels</subject><subject>Nucleic acids</subject><subject>Peptides</subject><subject>Primates</subject><subject>Proteins</subject><subject>Public health</subject><subject>Research and Analysis Methods</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Saliva</subject><subject>Spleen</subject><subject>T cells</subject><subject>Tropical diseases</subject><subject>Vaccination</subject><subject>Vaccines</subject><subject>Vector-borne diseases</subject><subject>Viremia</subject><subject>Viruses</subject><subject>Zika virus</subject><subject>Zika Virus Infection - immunology</subject><subject>Zika Virus Infection - veterinary</subject><subject>γ-Interferon</subject><issn>1935-2735</issn><issn>1935-2727</issn><issn>1935-2735</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>DOA</sourceid><recordid>eNptkk1rGzEQhpfS0qRp_0FpBYXSi119a_cSMKEfgUAvOfUiZiWtLXdXcqW1Ief-8cr1Otgl6CAxeuad0aupqrcEzwlT5PM6blOAfr4Jo51jjGtM1bPqkjRMzKhi4vnJ-aJ6lfMaY9GImrysLhjFvCnny-rPAq38coV86JwZfdxmlP3gISCwLsSdTyUyPmwcogztChITaiE7i3ZgjA8Oua7zxrsw9g9ok-JYmIxMHIYY0ABpiNmVACzBhzyin_4XoIPqsWR4Xb3ooM_uzbRfVfdfv9zffJ_d_fh2e7O4mxlJ-ThzjFsQuK4x41x2NWFSATNUWttS6WjbYtbw1oBsgVjChLCSNdIYEFxiwa6q9wfZTR-znuzLmjKlFKYMq0LcHggbYa03yZf-H3QEr_8FYlpqSKM3vdPQtUxa3DpLKKeC1GCarjbAmLU1I6xoXU_Vtu3grCkGJejPRM9vgl_pZdxphYk4CHyaBFL8vXV51IPPxvU9BFe-qfQtuGJSEFrQD_-hT79uopZQHlDcj6Wu2YvqhSRSUK6wLNT8Caos6wZvYnCdL_GzhI8nCSsH_bjKsd_ufzafg_wAmhRzTq57NINgvR_pY9d6P9J6GumS9u7UyMek4wyzv24S9LI</recordid><startdate>20200201</startdate><enddate>20200201</enddate><creator>Luo, Shengxue</creator><creator>Zhao, Wei</creator><creator>Ma, Xiaorui</creator><creator>Zhang, Panli</creator><creator>Liu, Bochao</creator><creator>Zhang, Ling</creator><creator>Wang, Wenjing</creator><creator>Wang, Yuanzhan</creator><creator>Fu, Yongshui</creator><creator>Allain, Jean-Pierre</creator><creator>Li, Tingting</creator><creator>Li, Chengyao</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SS</scope><scope>7T2</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-5727-2179</orcidid></search><sort><creationdate>20200201</creationdate><title>A high infectious simian adenovirus type 23 vector based vaccine efficiently protects common marmosets against Zika virus infection</title><author>Luo, Shengxue ; Zhao, Wei ; Ma, Xiaorui ; Zhang, Panli ; Liu, Bochao ; Zhang, Ling ; Wang, Wenjing ; Wang, Yuanzhan ; Fu, Yongshui ; Allain, Jean-Pierre ; Li, Tingting ; Li, Chengyao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c624t-e34da508803446f81367a3c26ddb26e2bb0394bca6ba1d1355d6396cca546053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adenoviridae Infections - 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The small primate common marmosets are susceptible to ZIKV, mimicking key features of human infection. Here, a novel simian adenovirus type 23 vector-based vaccine expressing ZIKV pre-membrane-envelope proteins (Sad23L-prM-E) was produced in high infectious titer. Due to determination of immunogenicity in mice, a single-dose of 3×108 PFU Sad23L-prM-E vaccine was intramuscularly inoculated to marmosets. This vaccine raised antibody titers of 104.07 E-specific and 103.13 neutralizing antibody (NAb), as well as robust specific IFN-γ secreting T-cell response (1,219 SFCs/106 cells) to E peptides. The vaccinated marmosets, upon challenge with a high dose of ZIKV (105 PFU) six weeks post prime immunization, reduced viremia by more than 100 folds, and the low level of detectable viral RNA (<103 copies/ml) in blood, saliva, urine and feces was promptly eliminated when the secondary NAb (titer >103.66) and T-cell response (>726 SFCs/106 PBMCs) were acquired 1-2 weeks post exposure to ZIKV, while non-vaccinated control marmosets developed long-term high titer of ZIKV (105.73 copies/ml) (P<0.05). No significant pathological lesions were observed in marmoset tissues. Sad23L-prM-E vaccine was detectable in spleen, liver and PBMCs at least 4 months post challenge. In conclusion, a prime immunization with Sad23L-prM-E vaccine was able to protect marmosets against ZIKV infection when exposed to a high dose of ZIKV. This Sad23L-prM-E vaccine is a promising vaccine candidate for prevention of ZIKV infection in humans.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32049958</pmid><doi>10.1371/journal.pntd.0008027</doi><orcidid>https://orcid.org/0000-0001-5727-2179</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1935-2735 |
| ispartof | PLoS neglected tropical diseases, 2020-02, Vol.14 (2), p.e0008027-e0008027 |
| issn | 1935-2735 1935-2727 1935-2735 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central Open Access; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Adenoviridae Infections - immunology Adenoviridae Infections - veterinary Adenoviridae Infections - virology Adenoviruses Adenoviruses, Simian - classification Animals Antibodies Biology and life sciences Biopharmaceuticals Biotechnology Callithrix Complications Disease control Immunization Immunogenicity Infection Infections Inoculation Laboratories Lesions Low level Lymphocytes T Medicine Medicine and Health Sciences Mimicry Monkey Diseases - immunology Monkey Diseases - virology Neurological complications Novels Nucleic acids Peptides Primates Proteins Public health Research and Analysis Methods Ribonucleic acid RNA Saliva Spleen T cells Tropical diseases Vaccination Vaccines Vector-borne diseases Viremia Viruses Zika virus Zika Virus Infection - immunology Zika Virus Infection - veterinary γ-Interferon |
| title | A high infectious simian adenovirus type 23 vector based vaccine efficiently protects common marmosets against Zika virus infection |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T04%3A42%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20high%20infectious%20simian%20adenovirus%20type%2023%20vector%20based%20vaccine%20efficiently%20protects%20common%20marmosets%20against%20Zika%20virus%20infection&rft.jtitle=PLoS%20neglected%20tropical%20diseases&rft.au=Luo,%20Shengxue&rft.date=2020-02-01&rft.volume=14&rft.issue=2&rft.spage=e0008027&rft.epage=e0008027&rft.pages=e0008027-e0008027&rft.issn=1935-2735&rft.eissn=1935-2735&rft_id=info:doi/10.1371/journal.pntd.0008027&rft_dat=%3Cgale_plos_%3EA616524706%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2377702307&rft_id=info:pmid/32049958&rft_galeid=A616524706&rft_doaj_id=oai_doaj_org_article_afb36d0bed1242518ac9f8ca33dd8313&rfr_iscdi=true |