Characterization of the immune cell landscape of patients with NAFLD
Multiple factors are involved in the pathogenesis of non-alcoholic fatty liver disease (NAFLD), but the exact immunological mechanisms that cause inflammation and fibrosis of the liver remain enigmatic. In this current study, cellular samples of a cohort of NAFLD patients (peripheral blood mononucle...
Gespeichert in:
| Veröffentlicht in: | PloS one 2020-03, Vol.15 (3), p.e0230307-e0230307 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0230307 |
|---|---|
| container_issue | 3 |
| container_start_page | e0230307 |
| container_title | PloS one |
| container_volume | 15 |
| creator | Diedrich, Tom Kummer, Silke Galante, Antonio Drolz, Andreas Schlicker, Veronika Lohse, Ansgar W Kluwe, Johannes Eberhard, Johanna Maria Schulze Zur Wiesch, Julian |
| description | Multiple factors are involved in the pathogenesis of non-alcoholic fatty liver disease (NAFLD), but the exact immunological mechanisms that cause inflammation and fibrosis of the liver remain enigmatic. In this current study, cellular samples of a cohort of NAFLD patients (peripheral blood mononuclear cells (PBMC): n = 27, liver samples: n = 15) and healthy individuals (PBMC: n = 26, liver samples: n = 3) were analyzed using 16-color flow cytometry, and the frequency and phenotype of 23 immune cell subtypes was assessed. PBMC of NAFLD patients showed decreased frequencies of total CD3+, CD8+ T cells, CD56dim NK cells and MAIT cells, but elevated frequencies of CD4+ T cells and Th2 cells compared to healthy controls. Intrahepatic lymphocytes (IHL) of NAFLD patients showed decreased frequencies of total T cells, total CD8+ T cells, Vd2+γδ T cells, and CD56bright NK cells, but elevated frequencies of Vδ2-γδ T cells and CD56dim NK cells compared to healthy controls. The activating receptor NKG2D was significantly less frequently expressed among iNKT cells, total NK cells and CD56dim NK cells of PBMC of NAFLD patients compared to healthy controls. More strikingly, hepatic fibrosis as measured by fibroscan elastography negatively correlated with the intrahepatic frequency of total NK cells (r2 = 0,3737, p = 0,02). Hepatic steatosis as measured by controlled attenuation parameter (CAP) value negatively correlated with the frequency of circulating NKG2D+ iNKT cells (r2 = 0,3365, p = 0,0047). Our data provide an overview of the circulating and intrahepatic immune cell composition of NAFLD patients, and point towards a potential role of NK cells and iNKT cells for the regulation of hepatic fibrosis and steatosis in NAFLD. |
| doi_str_mv | 10.1371/journal.pone.0230307 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2376946146</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A617357532</galeid><doaj_id>oai_doaj_org_article_7fd18f23a4be413c861b446dc162f973</doaj_id><sourcerecordid>A617357532</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-6f318952f0926ba5b5a2398cc26b87cf481a05480a309b1e74308bad9b66e9973</originalsourceid><addsrcrecordid>eNqNkktv1DAUhSMEoqXwDxBEQkKwmMGvOPYGaTSlMNKISry2luPYE1dJPNgOr1-P00mrCeoCZZHY_u45vjcny55CsIS4hG-u3OB72S73rtdLgDDAoLyXnUKO0YIigO8ffZ9kj0K4AqDAjNKH2QlGkDJMitPsfN1IL1XU3v6R0bo-dyaPjc5t1w29zpVu27yVfR2U3OvxcJ8w3ceQ_7SxyT-uLrbnj7MHRrZBP5neZ9nXi3df1h8W28v3m_Vqu1CUo7igBkPGC2QAR7SSRVVIhDlTKq1YqQxhUIKCMCAx4BXUJcGAVbLmFaWa8xKfZc8PuvvWBTENIAiES8oJhYQmYnMgaievxN7bTvrfwkkrrjec3wnpo1WtFqWpITMIS1JpArFiFFaE0FpBikwyS1pvJ7eh6nStUtNetjPR-UlvG7FzP0QJKKcIJYFXk4B33wcdouhsGAcqe-2G63snG0LYeO8X_6B3dzdRO5kasL1xyVeNomJFYYmLssCj7fIOKj217qxKaTE27c8KXs8KEhP1r7iTQwhi8_nT_7OX3-bsyyO20bKNTXDtMMYszEFyAJV3IXhtbocMgRjDfjMNMYZdTGFPZc-Of9Bt0U268V8qi_b0</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2376946146</pqid></control><display><type>article</type><title>Characterization of the immune cell landscape of patients with NAFLD</title><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Diedrich, Tom ; Kummer, Silke ; Galante, Antonio ; Drolz, Andreas ; Schlicker, Veronika ; Lohse, Ansgar W ; Kluwe, Johannes ; Eberhard, Johanna Maria ; Schulze Zur Wiesch, Julian</creator><creatorcontrib>Diedrich, Tom ; Kummer, Silke ; Galante, Antonio ; Drolz, Andreas ; Schlicker, Veronika ; Lohse, Ansgar W ; Kluwe, Johannes ; Eberhard, Johanna Maria ; Schulze Zur Wiesch, Julian</creatorcontrib><description>Multiple factors are involved in the pathogenesis of non-alcoholic fatty liver disease (NAFLD), but the exact immunological mechanisms that cause inflammation and fibrosis of the liver remain enigmatic. In this current study, cellular samples of a cohort of NAFLD patients (peripheral blood mononuclear cells (PBMC): n = 27, liver samples: n = 15) and healthy individuals (PBMC: n = 26, liver samples: n = 3) were analyzed using 16-color flow cytometry, and the frequency and phenotype of 23 immune cell subtypes was assessed. PBMC of NAFLD patients showed decreased frequencies of total CD3+, CD8+ T cells, CD56dim NK cells and MAIT cells, but elevated frequencies of CD4+ T cells and Th2 cells compared to healthy controls. Intrahepatic lymphocytes (IHL) of NAFLD patients showed decreased frequencies of total T cells, total CD8+ T cells, Vd2+γδ T cells, and CD56bright NK cells, but elevated frequencies of Vδ2-γδ T cells and CD56dim NK cells compared to healthy controls. The activating receptor NKG2D was significantly less frequently expressed among iNKT cells, total NK cells and CD56dim NK cells of PBMC of NAFLD patients compared to healthy controls. More strikingly, hepatic fibrosis as measured by fibroscan elastography negatively correlated with the intrahepatic frequency of total NK cells (r2 = 0,3737, p = 0,02). Hepatic steatosis as measured by controlled attenuation parameter (CAP) value negatively correlated with the frequency of circulating NKG2D+ iNKT cells (r2 = 0,3365, p = 0,0047). Our data provide an overview of the circulating and intrahepatic immune cell composition of NAFLD patients, and point towards a potential role of NK cells and iNKT cells for the regulation of hepatic fibrosis and steatosis in NAFLD.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0230307</identifier><identifier>PMID: 32168345</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Attenuation ; Biology and Life Sciences ; Biopsy ; CD3 antigen ; CD4 antigen ; CD8 antigen ; Characterization ; Cloning ; Compensation ; Correlation analysis ; Cytotoxicity ; Development and progression ; Diseases ; Fatty liver ; Fibrosis ; Flow cytometry ; Genotype & phenotype ; Health care ; Helper cells ; Immune system ; Immunology ; Inflammation ; Insulin resistance ; Killer cells ; Leukocytes (mononuclear) ; Liver ; Liver cancer ; Liver diseases ; Lymphocytes ; Lymphocytes T ; Medical research ; Medicine and Health Sciences ; NKG2 antigen ; Pathogenesis ; Peripheral blood mononuclear cells ; Phenotypes ; Research and Analysis Methods ; Steatosis ; T cells ; Ultrasonic imaging</subject><ispartof>PloS one, 2020-03, Vol.15 (3), p.e0230307-e0230307</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Diedrich et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Diedrich et al 2020 Diedrich et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-6f318952f0926ba5b5a2398cc26b87cf481a05480a309b1e74308bad9b66e9973</citedby><cites>FETCH-LOGICAL-c692t-6f318952f0926ba5b5a2398cc26b87cf481a05480a309b1e74308bad9b66e9973</cites><orcidid>0000-0002-5033-1938</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069622/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069622/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32168345$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Diedrich, Tom</creatorcontrib><creatorcontrib>Kummer, Silke</creatorcontrib><creatorcontrib>Galante, Antonio</creatorcontrib><creatorcontrib>Drolz, Andreas</creatorcontrib><creatorcontrib>Schlicker, Veronika</creatorcontrib><creatorcontrib>Lohse, Ansgar W</creatorcontrib><creatorcontrib>Kluwe, Johannes</creatorcontrib><creatorcontrib>Eberhard, Johanna Maria</creatorcontrib><creatorcontrib>Schulze Zur Wiesch, Julian</creatorcontrib><title>Characterization of the immune cell landscape of patients with NAFLD</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Multiple factors are involved in the pathogenesis of non-alcoholic fatty liver disease (NAFLD), but the exact immunological mechanisms that cause inflammation and fibrosis of the liver remain enigmatic. In this current study, cellular samples of a cohort of NAFLD patients (peripheral blood mononuclear cells (PBMC): n = 27, liver samples: n = 15) and healthy individuals (PBMC: n = 26, liver samples: n = 3) were analyzed using 16-color flow cytometry, and the frequency and phenotype of 23 immune cell subtypes was assessed. PBMC of NAFLD patients showed decreased frequencies of total CD3+, CD8+ T cells, CD56dim NK cells and MAIT cells, but elevated frequencies of CD4+ T cells and Th2 cells compared to healthy controls. Intrahepatic lymphocytes (IHL) of NAFLD patients showed decreased frequencies of total T cells, total CD8+ T cells, Vd2+γδ T cells, and CD56bright NK cells, but elevated frequencies of Vδ2-γδ T cells and CD56dim NK cells compared to healthy controls. The activating receptor NKG2D was significantly less frequently expressed among iNKT cells, total NK cells and CD56dim NK cells of PBMC of NAFLD patients compared to healthy controls. More strikingly, hepatic fibrosis as measured by fibroscan elastography negatively correlated with the intrahepatic frequency of total NK cells (r2 = 0,3737, p = 0,02). Hepatic steatosis as measured by controlled attenuation parameter (CAP) value negatively correlated with the frequency of circulating NKG2D+ iNKT cells (r2 = 0,3365, p = 0,0047). Our data provide an overview of the circulating and intrahepatic immune cell composition of NAFLD patients, and point towards a potential role of NK cells and iNKT cells for the regulation of hepatic fibrosis and steatosis in NAFLD.</description><subject>Analysis</subject><subject>Attenuation</subject><subject>Biology and Life Sciences</subject><subject>Biopsy</subject><subject>CD3 antigen</subject><subject>CD4 antigen</subject><subject>CD8 antigen</subject><subject>Characterization</subject><subject>Cloning</subject><subject>Compensation</subject><subject>Correlation analysis</subject><subject>Cytotoxicity</subject><subject>Development and progression</subject><subject>Diseases</subject><subject>Fatty liver</subject><subject>Fibrosis</subject><subject>Flow cytometry</subject><subject>Genotype & phenotype</subject><subject>Health care</subject><subject>Helper cells</subject><subject>Immune system</subject><subject>Immunology</subject><subject>Inflammation</subject><subject>Insulin resistance</subject><subject>Killer cells</subject><subject>Leukocytes (mononuclear)</subject><subject>Liver</subject><subject>Liver cancer</subject><subject>Liver diseases</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Medical research</subject><subject>Medicine and Health Sciences</subject><subject>NKG2 antigen</subject><subject>Pathogenesis</subject><subject>Peripheral blood mononuclear cells</subject><subject>Phenotypes</subject><subject>Research and Analysis Methods</subject><subject>Steatosis</subject><subject>T cells</subject><subject>Ultrasonic imaging</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkktv1DAUhSMEoqXwDxBEQkKwmMGvOPYGaTSlMNKISry2luPYE1dJPNgOr1-P00mrCeoCZZHY_u45vjcny55CsIS4hG-u3OB72S73rtdLgDDAoLyXnUKO0YIigO8ffZ9kj0K4AqDAjNKH2QlGkDJMitPsfN1IL1XU3v6R0bo-dyaPjc5t1w29zpVu27yVfR2U3OvxcJ8w3ceQ_7SxyT-uLrbnj7MHRrZBP5neZ9nXi3df1h8W28v3m_Vqu1CUo7igBkPGC2QAR7SSRVVIhDlTKq1YqQxhUIKCMCAx4BXUJcGAVbLmFaWa8xKfZc8PuvvWBTENIAiES8oJhYQmYnMgaievxN7bTvrfwkkrrjec3wnpo1WtFqWpITMIS1JpArFiFFaE0FpBikwyS1pvJ7eh6nStUtNetjPR-UlvG7FzP0QJKKcIJYFXk4B33wcdouhsGAcqe-2G63snG0LYeO8X_6B3dzdRO5kasL1xyVeNomJFYYmLssCj7fIOKj217qxKaTE27c8KXs8KEhP1r7iTQwhi8_nT_7OX3-bsyyO20bKNTXDtMMYszEFyAJV3IXhtbocMgRjDfjMNMYZdTGFPZc-Of9Bt0U268V8qi_b0</recordid><startdate>20200313</startdate><enddate>20200313</enddate><creator>Diedrich, Tom</creator><creator>Kummer, Silke</creator><creator>Galante, Antonio</creator><creator>Drolz, Andreas</creator><creator>Schlicker, Veronika</creator><creator>Lohse, Ansgar W</creator><creator>Kluwe, Johannes</creator><creator>Eberhard, Johanna Maria</creator><creator>Schulze Zur Wiesch, Julian</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-5033-1938</orcidid></search><sort><creationdate>20200313</creationdate><title>Characterization of the immune cell landscape of patients with NAFLD</title><author>Diedrich, Tom ; Kummer, Silke ; Galante, Antonio ; Drolz, Andreas ; Schlicker, Veronika ; Lohse, Ansgar W ; Kluwe, Johannes ; Eberhard, Johanna Maria ; Schulze Zur Wiesch, Julian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-6f318952f0926ba5b5a2398cc26b87cf481a05480a309b1e74308bad9b66e9973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Analysis</topic><topic>Attenuation</topic><topic>Biology and Life Sciences</topic><topic>Biopsy</topic><topic>CD3 antigen</topic><topic>CD4 antigen</topic><topic>CD8 antigen</topic><topic>Characterization</topic><topic>Cloning</topic><topic>Compensation</topic><topic>Correlation analysis</topic><topic>Cytotoxicity</topic><topic>Development and progression</topic><topic>Diseases</topic><topic>Fatty liver</topic><topic>Fibrosis</topic><topic>Flow cytometry</topic><topic>Genotype & phenotype</topic><topic>Health care</topic><topic>Helper cells</topic><topic>Immune system</topic><topic>Immunology</topic><topic>Inflammation</topic><topic>Insulin resistance</topic><topic>Killer cells</topic><topic>Leukocytes (mononuclear)</topic><topic>Liver</topic><topic>Liver cancer</topic><topic>Liver diseases</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Medical research</topic><topic>Medicine and Health Sciences</topic><topic>NKG2 antigen</topic><topic>Pathogenesis</topic><topic>Peripheral blood mononuclear cells</topic><topic>Phenotypes</topic><topic>Research and Analysis Methods</topic><topic>Steatosis</topic><topic>T cells</topic><topic>Ultrasonic imaging</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Diedrich, Tom</creatorcontrib><creatorcontrib>Kummer, Silke</creatorcontrib><creatorcontrib>Galante, Antonio</creatorcontrib><creatorcontrib>Drolz, Andreas</creatorcontrib><creatorcontrib>Schlicker, Veronika</creatorcontrib><creatorcontrib>Lohse, Ansgar W</creatorcontrib><creatorcontrib>Kluwe, Johannes</creatorcontrib><creatorcontrib>Eberhard, Johanna Maria</creatorcontrib><creatorcontrib>Schulze Zur Wiesch, Julian</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Diedrich, Tom</au><au>Kummer, Silke</au><au>Galante, Antonio</au><au>Drolz, Andreas</au><au>Schlicker, Veronika</au><au>Lohse, Ansgar W</au><au>Kluwe, Johannes</au><au>Eberhard, Johanna Maria</au><au>Schulze Zur Wiesch, Julian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the immune cell landscape of patients with NAFLD</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2020-03-13</date><risdate>2020</risdate><volume>15</volume><issue>3</issue><spage>e0230307</spage><epage>e0230307</epage><pages>e0230307-e0230307</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Multiple factors are involved in the pathogenesis of non-alcoholic fatty liver disease (NAFLD), but the exact immunological mechanisms that cause inflammation and fibrosis of the liver remain enigmatic. In this current study, cellular samples of a cohort of NAFLD patients (peripheral blood mononuclear cells (PBMC): n = 27, liver samples: n = 15) and healthy individuals (PBMC: n = 26, liver samples: n = 3) were analyzed using 16-color flow cytometry, and the frequency and phenotype of 23 immune cell subtypes was assessed. PBMC of NAFLD patients showed decreased frequencies of total CD3+, CD8+ T cells, CD56dim NK cells and MAIT cells, but elevated frequencies of CD4+ T cells and Th2 cells compared to healthy controls. Intrahepatic lymphocytes (IHL) of NAFLD patients showed decreased frequencies of total T cells, total CD8+ T cells, Vd2+γδ T cells, and CD56bright NK cells, but elevated frequencies of Vδ2-γδ T cells and CD56dim NK cells compared to healthy controls. The activating receptor NKG2D was significantly less frequently expressed among iNKT cells, total NK cells and CD56dim NK cells of PBMC of NAFLD patients compared to healthy controls. More strikingly, hepatic fibrosis as measured by fibroscan elastography negatively correlated with the intrahepatic frequency of total NK cells (r2 = 0,3737, p = 0,02). Hepatic steatosis as measured by controlled attenuation parameter (CAP) value negatively correlated with the frequency of circulating NKG2D+ iNKT cells (r2 = 0,3365, p = 0,0047). Our data provide an overview of the circulating and intrahepatic immune cell composition of NAFLD patients, and point towards a potential role of NK cells and iNKT cells for the regulation of hepatic fibrosis and steatosis in NAFLD.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32168345</pmid><doi>10.1371/journal.pone.0230307</doi><tpages>e0230307</tpages><orcidid>https://orcid.org/0000-0002-5033-1938</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2020-03, Vol.15 (3), p.e0230307-e0230307 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2376946146 |
| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Analysis Attenuation Biology and Life Sciences Biopsy CD3 antigen CD4 antigen CD8 antigen Characterization Cloning Compensation Correlation analysis Cytotoxicity Development and progression Diseases Fatty liver Fibrosis Flow cytometry Genotype & phenotype Health care Helper cells Immune system Immunology Inflammation Insulin resistance Killer cells Leukocytes (mononuclear) Liver Liver cancer Liver diseases Lymphocytes Lymphocytes T Medical research Medicine and Health Sciences NKG2 antigen Pathogenesis Peripheral blood mononuclear cells Phenotypes Research and Analysis Methods Steatosis T cells Ultrasonic imaging |
| title | Characterization of the immune cell landscape of patients with NAFLD |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T13%3A55%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20the%20immune%20cell%20landscape%20of%20patients%20with%20NAFLD&rft.jtitle=PloS%20one&rft.au=Diedrich,%20Tom&rft.date=2020-03-13&rft.volume=15&rft.issue=3&rft.spage=e0230307&rft.epage=e0230307&rft.pages=e0230307-e0230307&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0230307&rft_dat=%3Cgale_plos_%3EA617357532%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2376946146&rft_id=info:pmid/32168345&rft_galeid=A617357532&rft_doaj_id=oai_doaj_org_article_7fd18f23a4be413c861b446dc162f973&rfr_iscdi=true |