Dietary supplementation of recombinant antimicrobial peptide Epinephelus lanceolatus piscidin improves growth performance and immune response in Gallus gallus domesticus
Supplementing chicken feed with antibiotics can improve survival and prevent disease outbreaks. However, overuse of antibiotics may promote the development of antibiotic-resistant bacteria. Recently, antimicrobial peptides have been proposed as alternatives to antibiotics in animal husbandry. Here,...
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| creator | Tai, Hsueh-Ming Huang, Han-Ning Tsai, Tsung-Yu You, Ming-Feng Wu, Hung-Yi Rajanbabu, Venugopal Chang, Hsiao-Yun Pan, Chieh-Yu Chen, Jyh-Yih |
| description | Supplementing chicken feed with antibiotics can improve survival and prevent disease outbreaks. However, overuse of antibiotics may promote the development of antibiotic-resistant bacteria. Recently, antimicrobial peptides have been proposed as alternatives to antibiotics in animal husbandry. Here, we evaluate the effects of antimicrobial peptide, Epinephelus lanceolatus piscidin (EP), in Gallus gallus domesticus. The gene encoding EP was isolated, sequenced, codon-optimized and cloned into a Pichia pastoris recombinant protein expression system. The expressed recombinant EP (rEP) was then used as a dietary supplement for G. g. domesticus; overall health, growth performance and immunity were assessed. Supernatant from rEP-expressing yeast showed in vitro antimicrobial activity against Gram-positive and Gram-negative bacteria, according to an inhibition-zone diameter (mm) assay. Moreover, the antimicrobial peptide function of rEP was temperature independent. The fermentation broth yielded a spray-dried powder formulation containing 262.9 μg EP/g powder, and LC-MS/MS (tandem MS) analysis confirmed that rEP had a molecular weight of 4279 Da, as expected for the 34-amino acid peptide; the DNA sequence of the expression vector was also validated. We then evaluated rEP as a feed additive for G. g. domesticus. Treatment groups included control, basal diet and rEP at different doses (0.75, 1.5, 3.0, 6.0 and 12%). Compared to control, rEP supplementation increased G. g. domesticus weight gain, feed efficiency, IL-10 and IFN-γ production. Our results suggest that crude rEP could provide an alternative to traditional antibiotic feed additives for G. g. domesticus, serving to enhance growth and health of the animals. |
| doi_str_mv | 10.1371/journal.pone.0230021 |
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However, overuse of antibiotics may promote the development of antibiotic-resistant bacteria. Recently, antimicrobial peptides have been proposed as alternatives to antibiotics in animal husbandry. Here, we evaluate the effects of antimicrobial peptide, Epinephelus lanceolatus piscidin (EP), in Gallus gallus domesticus. The gene encoding EP was isolated, sequenced, codon-optimized and cloned into a Pichia pastoris recombinant protein expression system. The expressed recombinant EP (rEP) was then used as a dietary supplement for G. g. domesticus; overall health, growth performance and immunity were assessed. Supernatant from rEP-expressing yeast showed in vitro antimicrobial activity against Gram-positive and Gram-negative bacteria, according to an inhibition-zone diameter (mm) assay. Moreover, the antimicrobial peptide function of rEP was temperature independent. The fermentation broth yielded a spray-dried powder formulation containing 262.9 μg EP/g powder, and LC-MS/MS (tandem MS) analysis confirmed that rEP had a molecular weight of 4279 Da, as expected for the 34-amino acid peptide; the DNA sequence of the expression vector was also validated. We then evaluated rEP as a feed additive for G. g. domesticus. Treatment groups included control, basal diet and rEP at different doses (0.75, 1.5, 3.0, 6.0 and 12%). Compared to control, rEP supplementation increased G. g. domesticus weight gain, feed efficiency, IL-10 and IFN-γ production. Our results suggest that crude rEP could provide an alternative to traditional antibiotic feed additives for G. g. domesticus, serving to enhance growth and health of the animals.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0230021</identifier><identifier>PMID: 32160226</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Additives ; Amino acid sequence ; Amino acids ; Animal feeding and feeds ; Animal husbandry ; Antibiotic resistance ; Antibiotics ; Antiinfectives and antibacterials ; Antimicrobial activity ; Antimicrobial agents ; Antimicrobial peptides ; Bacteria ; Bacterial infections ; Base sequence ; Biology ; Biology and Life Sciences ; Biotechnology ; Body weight gain ; Cloning ; Codons ; Deoxyribonucleic acid ; Diet ; Dietary supplements ; Diseases ; DNA ; DNA sequencing ; Epidemics ; Epinephelus lanceolatus ; Feed additives ; Feed conversion ; Feed efficiency ; Feeds ; Fermentation ; Fishes ; Food additives ; Gallus gallus domesticus ; Gene expression ; Genes ; Genetic engineering ; Gram-negative bacteria ; Growth ; Health ; Immune response ; Immune system ; Interleukin 10 ; Legal fees ; Medicine and Health Sciences ; Methicillin ; Microbial drug resistance ; Microbiota ; Molecular weight ; Nucleotide sequence ; Pathogens ; Peptides ; Poultry ; Poultry feed ; Powders (Particulate matter) ; Protein expression ; Proteins ; Recombinant proteins ; Research and Analysis Methods ; Veterinary medicine ; Yeasts ; γ-Interferon</subject><ispartof>PloS one, 2020-03, Vol.15 (3), p.e0230021-e0230021</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Tai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Tai et al 2020 Tai et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-ee7712bbea9bc747256cdf92b0f541c5442a3bb194ca39bccee40f3ad38538973</citedby><cites>FETCH-LOGICAL-c692t-ee7712bbea9bc747256cdf92b0f541c5442a3bb194ca39bccee40f3ad38538973</cites><orcidid>0000-0001-8761-3333 ; 0000-0001-7858-8236</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065771/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065771/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32160226$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tai, Hsueh-Ming</creatorcontrib><creatorcontrib>Huang, Han-Ning</creatorcontrib><creatorcontrib>Tsai, Tsung-Yu</creatorcontrib><creatorcontrib>You, Ming-Feng</creatorcontrib><creatorcontrib>Wu, Hung-Yi</creatorcontrib><creatorcontrib>Rajanbabu, Venugopal</creatorcontrib><creatorcontrib>Chang, Hsiao-Yun</creatorcontrib><creatorcontrib>Pan, Chieh-Yu</creatorcontrib><creatorcontrib>Chen, Jyh-Yih</creatorcontrib><title>Dietary supplementation of recombinant antimicrobial peptide Epinephelus lanceolatus piscidin improves growth performance and immune response in Gallus gallus domesticus</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Supplementing chicken feed with antibiotics can improve survival and prevent disease outbreaks. However, overuse of antibiotics may promote the development of antibiotic-resistant bacteria. Recently, antimicrobial peptides have been proposed as alternatives to antibiotics in animal husbandry. Here, we evaluate the effects of antimicrobial peptide, Epinephelus lanceolatus piscidin (EP), in Gallus gallus domesticus. The gene encoding EP was isolated, sequenced, codon-optimized and cloned into a Pichia pastoris recombinant protein expression system. The expressed recombinant EP (rEP) was then used as a dietary supplement for G. g. domesticus; overall health, growth performance and immunity were assessed. Supernatant from rEP-expressing yeast showed in vitro antimicrobial activity against Gram-positive and Gram-negative bacteria, according to an inhibition-zone diameter (mm) assay. Moreover, the antimicrobial peptide function of rEP was temperature independent. The fermentation broth yielded a spray-dried powder formulation containing 262.9 μg EP/g powder, and LC-MS/MS (tandem MS) analysis confirmed that rEP had a molecular weight of 4279 Da, as expected for the 34-amino acid peptide; the DNA sequence of the expression vector was also validated. We then evaluated rEP as a feed additive for G. g. domesticus. Treatment groups included control, basal diet and rEP at different doses (0.75, 1.5, 3.0, 6.0 and 12%). Compared to control, rEP supplementation increased G. g. domesticus weight gain, feed efficiency, IL-10 and IFN-γ production. Our results suggest that crude rEP could provide an alternative to traditional antibiotic feed additives for G. g. domesticus, serving to enhance growth and health of the animals.</description><subject>Additives</subject><subject>Amino acid sequence</subject><subject>Amino acids</subject><subject>Animal feeding and feeds</subject><subject>Animal husbandry</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial activity</subject><subject>Antimicrobial agents</subject><subject>Antimicrobial peptides</subject><subject>Bacteria</subject><subject>Bacterial infections</subject><subject>Base sequence</subject><subject>Biology</subject><subject>Biology and Life Sciences</subject><subject>Biotechnology</subject><subject>Body weight gain</subject><subject>Cloning</subject><subject>Codons</subject><subject>Deoxyribonucleic acid</subject><subject>Diet</subject><subject>Dietary supplements</subject><subject>Diseases</subject><subject>DNA</subject><subject>DNA sequencing</subject><subject>Epidemics</subject><subject>Epinephelus lanceolatus</subject><subject>Feed additives</subject><subject>Feed conversion</subject><subject>Feed efficiency</subject><subject>Feeds</subject><subject>Fermentation</subject><subject>Fishes</subject><subject>Food additives</subject><subject>Gallus gallus domesticus</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Gram-negative bacteria</subject><subject>Growth</subject><subject>Health</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Interleukin 10</subject><subject>Legal fees</subject><subject>Medicine and Health Sciences</subject><subject>Methicillin</subject><subject>Microbial drug resistance</subject><subject>Microbiota</subject><subject>Molecular weight</subject><subject>Nucleotide sequence</subject><subject>Pathogens</subject><subject>Peptides</subject><subject>Poultry</subject><subject>Poultry feed</subject><subject>Powders (Particulate matter)</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Recombinant proteins</subject><subject>Research and Analysis Methods</subject><subject>Veterinary 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peptide Epinephelus lanceolatus piscidin improves growth performance and immune response in Gallus gallus domesticus</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2020-03-11</date><risdate>2020</risdate><volume>15</volume><issue>3</issue><spage>e0230021</spage><epage>e0230021</epage><pages>e0230021-e0230021</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Supplementing chicken feed with antibiotics can improve survival and prevent disease outbreaks. However, overuse of antibiotics may promote the development of antibiotic-resistant bacteria. Recently, antimicrobial peptides have been proposed as alternatives to antibiotics in animal husbandry. Here, we evaluate the effects of antimicrobial peptide, Epinephelus lanceolatus piscidin (EP), in Gallus gallus domesticus. The gene encoding EP was isolated, sequenced, codon-optimized and cloned into a Pichia pastoris recombinant protein expression system. The expressed recombinant EP (rEP) was then used as a dietary supplement for G. g. domesticus; overall health, growth performance and immunity were assessed. Supernatant from rEP-expressing yeast showed in vitro antimicrobial activity against Gram-positive and Gram-negative bacteria, according to an inhibition-zone diameter (mm) assay. Moreover, the antimicrobial peptide function of rEP was temperature independent. The fermentation broth yielded a spray-dried powder formulation containing 262.9 μg EP/g powder, and LC-MS/MS (tandem MS) analysis confirmed that rEP had a molecular weight of 4279 Da, as expected for the 34-amino acid peptide; the DNA sequence of the expression vector was also validated. We then evaluated rEP as a feed additive for G. g. domesticus. Treatment groups included control, basal diet and rEP at different doses (0.75, 1.5, 3.0, 6.0 and 12%). Compared to control, rEP supplementation increased G. g. domesticus weight gain, feed efficiency, IL-10 and IFN-γ production. Our results suggest that crude rEP could provide an alternative to traditional antibiotic feed additives for G. g. domesticus, serving to enhance growth and health of the animals.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32160226</pmid><doi>10.1371/journal.pone.0230021</doi><orcidid>https://orcid.org/0000-0001-8761-3333</orcidid><orcidid>https://orcid.org/0000-0001-7858-8236</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2020-03, Vol.15 (3), p.e0230021-e0230021 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2376207249 |
| source | Public Library of Science (PLoS) Journals Open Access; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Additives Amino acid sequence Amino acids Animal feeding and feeds Animal husbandry Antibiotic resistance Antibiotics Antiinfectives and antibacterials Antimicrobial activity Antimicrobial agents Antimicrobial peptides Bacteria Bacterial infections Base sequence Biology Biology and Life Sciences Biotechnology Body weight gain Cloning Codons Deoxyribonucleic acid Diet Dietary supplements Diseases DNA DNA sequencing Epidemics Epinephelus lanceolatus Feed additives Feed conversion Feed efficiency Feeds Fermentation Fishes Food additives Gallus gallus domesticus Gene expression Genes Genetic engineering Gram-negative bacteria Growth Health Immune response Immune system Interleukin 10 Legal fees Medicine and Health Sciences Methicillin Microbial drug resistance Microbiota Molecular weight Nucleotide sequence Pathogens Peptides Poultry Poultry feed Powders (Particulate matter) Protein expression Proteins Recombinant proteins Research and Analysis Methods Veterinary medicine Yeasts γ-Interferon |
| title | Dietary supplementation of recombinant antimicrobial peptide Epinephelus lanceolatus piscidin improves growth performance and immune response in Gallus gallus domesticus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T14%3A40%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dietary%20supplementation%20of%20recombinant%20antimicrobial%20peptide%20Epinephelus%20lanceolatus%20piscidin%20improves%20growth%20performance%20and%20immune%20response%20in%20Gallus%20gallus%20domesticus&rft.jtitle=PloS%20one&rft.au=Tai,%20Hsueh-Ming&rft.date=2020-03-11&rft.volume=15&rft.issue=3&rft.spage=e0230021&rft.epage=e0230021&rft.pages=e0230021-e0230021&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0230021&rft_dat=%3Cgale_plos_%3EA617101239%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2376207249&rft_id=info:pmid/32160226&rft_galeid=A617101239&rft_doaj_id=oai_doaj_org_article_e4e639a532004148b3f82b3c79bbd338&rfr_iscdi=true |