Development of a minigenome cassette for Lettuce necrotic yellows virus: A first step in rescuing a plant cytorhabdovirus

Rhabdoviruses are enveloped negative-sense RNA viruses that have numerous biotechnological applications. However, recovering plant rhabdoviruses from cDNA remains difficult due to technical difficulties such as the need for concurrent in planta expression of the viral genome together with the viral...

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Veröffentlicht in:	PloS one 2020-03, Vol.15 (3), p.e0229877-e0229877
Hauptverfasser:	Ibrahim, Ahmad E C, van Dolleweerd, Craig J, Drake, Pascal M W, Ma, Julian K-C
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium tumefaciens - genetics Binding sites Biology and Life Sciences Biotechnology Catalytic RNA Cloning Deoxyribonucleic acid DNA DNA, Complementary - genetics DNA-directed RNA polymerase E coli Escherichia coli Escherichia coli - genetics Fluorescence Gene expression Genes Genes, Viral Genome, Viral - genetics Genomes Genomic instability Genomics Glycoproteins Hepatitis Infections Introns Lettuce Luminescent Proteins - genetics Luminescent Proteins - metabolism Nicotiana - genetics Nicotiana - virology Nucleoproteins - genetics Open Reading Frames Peptides Phosphoproteins - genetics Plant Diseases - genetics Plant Diseases - virology Plasmids Plasmids - genetics Polymerase chain reaction Proteins Red fluorescent protein Research and Analysis Methods Rhabdoviridae - genetics Rhabdoviridae Infections - genetics Rhabdoviridae Infections - virology Ribonucleic acid Ribozymes RNA RNA polymerase RNA viruses RNA, Viral - genetics RNA, Viral - isolation & purification RNA-Dependent RNA Polymerase - genetics RNA-directed RNA polymerase Scientific equipment industry Sequence Analysis, DNA Splicing Viral Proteins - genetics Virus Replication - genetics Viruses Western blotting Yellows
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