A convenient renewable surface plasmon resonance chip for relative quantification of genetically modified soybean in food and feed
The cultivation of genetically modified organisms (GMO) continues to expand worldwide. Still, many consumers express concerns about the use of GMO in food or feed, and many countries have legislated on labelling systems to indicate the presence of GMO in commercial products. To deal with the increas...
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| Veröffentlicht in: | PloS one 2020-02, Vol.15 (2), p.e0229659-e0229659 |
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| creator | Plácido, Alexandra Ferreira-da-Silva, Frederico Leite, José Roberto S A de-Los-Santos-Álvarez, Noemí Delerue-Matos, Cristina |
| description | The cultivation of genetically modified organisms (GMO) continues to expand worldwide. Still, many consumers express concerns about the use of GMO in food or feed, and many countries have legislated on labelling systems to indicate the presence of GMO in commercial products. To deal with the increased number of GMO events and to address related regulations, alternative detection methods for GMO inspection are required. In this work, a genosensor based on Surface Plasmon Resonance under continuous flow was developed for the detection and quantification of a genetically modified soybean (event GTS 40-3-2). In a single chip, the simultaneous detection of the event-specific and the taxon-specific samples were achieved, whose detection limits were 20 pM and 16 pM, respectively. The reproducibility was 1.4%, which supports the use of the chip as a reliable and cost-effective alternative to other DNA-based techniques. The results indicate that the proposed method is a versatile tool for GMO quantification in food and feed samples. |
| doi_str_mv | 10.1371/journal.pone.0229659 |
| format | Article |
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Still, many consumers express concerns about the use of GMO in food or feed, and many countries have legislated on labelling systems to indicate the presence of GMO in commercial products. To deal with the increased number of GMO events and to address related regulations, alternative detection methods for GMO inspection are required. In this work, a genosensor based on Surface Plasmon Resonance under continuous flow was developed for the detection and quantification of a genetically modified soybean (event GTS 40-3-2). In a single chip, the simultaneous detection of the event-specific and the taxon-specific samples were achieved, whose detection limits were 20 pM and 16 pM, respectively. The reproducibility was 1.4%, which supports the use of the chip as a reliable and cost-effective alternative to other DNA-based techniques. 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Still, many consumers express concerns about the use of GMO in food or feed, and many countries have legislated on labelling systems to indicate the presence of GMO in commercial products. To deal with the increased number of GMO events and to address related regulations, alternative detection methods for GMO inspection are required. In this work, a genosensor based on Surface Plasmon Resonance under continuous flow was developed for the detection and quantification of a genetically modified soybean (event GTS 40-3-2). In a single chip, the simultaneous detection of the event-specific and the taxon-specific samples were achieved, whose detection limits were 20 pM and 16 pM, respectively. The reproducibility was 1.4%, which supports the use of the chip as a reliable and cost-effective alternative to other DNA-based techniques. The results indicate that the proposed method is a versatile tool for GMO quantification in food and feed samples.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32101588</pmid><doi>10.1371/journal.pone.0229659</doi><tpages>e0229659</tpages><orcidid>https://orcid.org/0000-0003-2706-7777</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Analytical methods Backup software Biology and Life Sciences Biosensors Continuous flow Crops Cultivation Deoxyribonucleic acid Detection limits DNA DNA, Plant - genetics EDTA Engineering and Technology Enzymes Experiments Food Food, Genetically Modified - classification Genetic modification Genetically altered foods Genetically engineered foods Genetically engineered organisms Genetically modified crops Genetically modified organisms Glycine max - genetics Hybridization Inspection Kinetics Labeling Labelling Legal fees Oligonucleotide Array Sequence Analysis - methods Organisms, Genetically Modified - chemistry Organisms, Genetically Modified - genetics Plants, Genetically Modified - genetics Proteins Reproducibility of Results Research and analysis methods Resonance Sensors Soybeans Surface plasmon resonance Surface Plasmon Resonance - methods Surfactants |
| title | A convenient renewable surface plasmon resonance chip for relative quantification of genetically modified soybean in food and feed |
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