Transcriptional analysis of wheat seedlings inoculated with Fusarium culmorum under continual exposure to disease defence inductors
A facultative parasite of cereals, Fusarium culmorum is a soil-, air- and seed-borne fungus causing foot and root rot, fusarium seedling blight, and especially Fusarium head blight, a spike disease leading to decreased yield and mycotoxin contamination of grain. In the present study, we tested chang...
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| creator | Antalová, Zuzana Bleša, Dominik Martinek, Petr Matušinsky, Pavel |
| description | A facultative parasite of cereals, Fusarium culmorum is a soil-, air- and seed-borne fungus causing foot and root rot, fusarium seedling blight, and especially Fusarium head blight, a spike disease leading to decreased yield and mycotoxin contamination of grain. In the present study, we tested changes in expression of wheat genes (B2H2, ICS, PAL, and PR2) involved in defence against diseases. We first compared expression of the analysed genes in seedlings of non-inoculated and artificially inoculated wheat (variety Bohemia). The second part of the experiment compared expression of these genes in seedlings grown under various treatment conditions. These treatments were chosen to determine the effects of prochloraz, sodium bicarbonate, ergosterol, aescin and potassium iodide on expression of the analysed defence genes. In addition to the inoculated and non-inoculated cultivar Bohemia, we additionally examined two other varieties of wheat with contrasting resistance to Fusarium sp. infection. These were the blue aleurone layer variety Scorpion that is susceptible to Fusarium sp. infection and variety V2-49-17 with yellow endosperm and partial resistance to Fusarium sp. infection. In this manner, we were able to compare potential effects of inductors upon defence gene expression among three varieties with different susceptibility to infection but also between inoculated and non-inoculated seedlings of a single variety. The lowest infection levels were detected in the sodium bicarbonate treatment. Sodium bicarbonate had not only negative influence on Fusarium growth but also positively affected expression of plant defence genes. Expression of the four marker genes shown to be important in plant defence was significantly affected by the treatments. The greatest upregulation in comparison to the water control was identified under all treatments for the B2H2 gene. Only expression of PAL under the ergosterol and prochloraz treatments were not statistically significant. |
| doi_str_mv | 10.1371/journal.pone.0224413 |
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In the present study, we tested changes in expression of wheat genes (B2H2, ICS, PAL, and PR2) involved in defence against diseases. We first compared expression of the analysed genes in seedlings of non-inoculated and artificially inoculated wheat (variety Bohemia). The second part of the experiment compared expression of these genes in seedlings grown under various treatment conditions. These treatments were chosen to determine the effects of prochloraz, sodium bicarbonate, ergosterol, aescin and potassium iodide on expression of the analysed defence genes. In addition to the inoculated and non-inoculated cultivar Bohemia, we additionally examined two other varieties of wheat with contrasting resistance to Fusarium sp. infection. These were the blue aleurone layer variety Scorpion that is susceptible to Fusarium sp. infection and variety V2-49-17 with yellow endosperm and partial resistance to Fusarium sp. infection. In this manner, we were able to compare potential effects of inductors upon defence gene expression among three varieties with different susceptibility to infection but also between inoculated and non-inoculated seedlings of a single variety. The lowest infection levels were detected in the sodium bicarbonate treatment. Sodium bicarbonate had not only negative influence on Fusarium growth but also positively affected expression of plant defence genes. Expression of the four marker genes shown to be important in plant defence was significantly affected by the treatments. The greatest upregulation in comparison to the water control was identified under all treatments for the B2H2 gene. Only expression of PAL under the ergosterol and prochloraz treatments were not statistically significant.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0224413</identifier><identifier>PMID: 32045412</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aleurone layer ; Analysis ; Biology and Life Sciences ; Blight ; Carbonates ; Cereals ; Chitinase ; Contamination ; Crop yield ; Cultivars ; Disease Resistance - genetics ; Disease susceptibility ; Diseases ; Electronic components ; Endosperm ; Ergosterol ; Experiments ; Fungi ; Fusarium ; Fusarium - immunology ; Fusarium culmorum ; Gene expression ; Gene Expression Profiling ; Genes ; Genetic aspects ; Halides ; Inductors ; Infections ; Iodides ; Medicine and Health Sciences ; Mycotoxins ; Parasites ; Physical Sciences ; Phytosterols ; Plant Diseases - genetics ; Plant Diseases - immunology ; Plant Diseases - microbiology ; Plant genetics ; Plant lipids ; Plant pathology ; Plant sciences ; Potassium ; Potassium iodide ; Potassium iodides ; Prochloraz ; Root rot ; Seed-borne fungi ; Seedling blight ; Seedlings ; Seedlings - genetics ; Seedlings - microbiology ; Seeds ; Sodium ; Sodium bicarbonate ; Sodium Bicarbonate - pharmacology ; Soil contamination ; Statistical analysis ; Transcription ; Transcription (Genetics) ; Triticum - genetics ; Water ; Water control ; Wheat</subject><ispartof>PloS one, 2020-02, Vol.15 (2), p.e0224413-e0224413</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Antalová et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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genetics</subject><subject>Plant Diseases - immunology</subject><subject>Plant Diseases - microbiology</subject><subject>Plant genetics</subject><subject>Plant lipids</subject><subject>Plant pathology</subject><subject>Plant sciences</subject><subject>Potassium</subject><subject>Potassium iodide</subject><subject>Potassium iodides</subject><subject>Prochloraz</subject><subject>Root rot</subject><subject>Seed-borne fungi</subject><subject>Seedling blight</subject><subject>Seedlings</subject><subject>Seedlings - genetics</subject><subject>Seedlings - microbiology</subject><subject>Seeds</subject><subject>Sodium</subject><subject>Sodium bicarbonate</subject><subject>Sodium Bicarbonate - pharmacology</subject><subject>Soil contamination</subject><subject>Statistical analysis</subject><subject>Transcription</subject><subject>Transcription (Genetics)</subject><subject>Triticum - genetics</subject><subject>Water</subject><subject>Water control</subject><subject>Wheat</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk01v1DAQhiMEoqXwDxBEQkJw2MUfiZNckKqKwkqVKkHhajn2ZNdVYm_9Qdszfxynm1Yb1AOK5IzGz7z2zHiy7DVGS0wr_OnSRmdEv9xaA0tESFFg-iQ7xA0lC0YQfbpnH2QvvL9EqKQ1Y8-zA0pQURaYHGZ_LpwwXjq9DdomuVyk5dZrn9suv96ACLkHUL02a59rY2XsRQCVX-uwyU-jF07HIU_ewbpkRKPA5dKaoE1ManCztT46yIPNlfYgPOQKOjASkpqKMljnX2bPOtF7eDX9j7Kfp18uTr4tzs6_rk6OzxaSNSQsmqqlDKWlkUJKXKqKgMQCQY3bZJXQVRWSVVkVNUBb06JpawGipiVLqUpFj7K3O91tbz2f6uc5oSUty5qUKBGrHaGsuORbpwfhbrkVmt85rFtz4YKWPXAmmxQATYFbVCjCWta2bdkJrBgiTSWS1ufptNgOoCSY4EQ_E53vGL3ha_ubVwgT2oyX-TAJOHsVwQc-aC-h74UBG-_uXeCmqGqW0Hf_oI9nN1FrkRLQprPpXDmK8mOGKWsQo3Wilo9Q6VMw6NRZ6HTyzwI-zgLG7sNNWIvoPV_9-P7_7PmvOft-j01PsQ8bb_s4PlQ_B4sdKJ313kH3UGSM-Dgr99Xg46zwaVZS2Jv9Bj0E3Q8H_QuutBID</recordid><startdate>20200211</startdate><enddate>20200211</enddate><creator>Antalová, Zuzana</creator><creator>Bleša, Dominik</creator><creator>Martinek, Petr</creator><creator>Matušinsky, Pavel</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-9095-3934</orcidid></search><sort><creationdate>20200211</creationdate><title>Transcriptional analysis of wheat seedlings inoculated with Fusarium culmorum under continual exposure to disease defence inductors</title><author>Antalová, Zuzana ; Bleša, Dominik ; Martinek, Petr ; Matušinsky, Pavel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-97b3607b39cacc15d72ec1a0e81b2ec5ef770c75748eeb8349b8aea8356412cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aleurone layer</topic><topic>Analysis</topic><topic>Biology and Life Sciences</topic><topic>Blight</topic><topic>Carbonates</topic><topic>Cereals</topic><topic>Chitinase</topic><topic>Contamination</topic><topic>Crop yield</topic><topic>Cultivars</topic><topic>Disease Resistance - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Antalová, Zuzana</au><au>Bleša, Dominik</au><au>Martinek, Petr</au><au>Matušinsky, Pavel</au><au>Sahu, Binod Bihari</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptional analysis of wheat seedlings inoculated with Fusarium culmorum under continual exposure to disease defence inductors</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2020-02-11</date><risdate>2020</risdate><volume>15</volume><issue>2</issue><spage>e0224413</spage><epage>e0224413</epage><pages>e0224413-e0224413</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>A facultative parasite of cereals, Fusarium culmorum is a soil-, air- and seed-borne fungus causing foot and root rot, fusarium seedling blight, and especially Fusarium head blight, a spike disease leading to decreased yield and mycotoxin contamination of grain. In the present study, we tested changes in expression of wheat genes (B2H2, ICS, PAL, and PR2) involved in defence against diseases. We first compared expression of the analysed genes in seedlings of non-inoculated and artificially inoculated wheat (variety Bohemia). The second part of the experiment compared expression of these genes in seedlings grown under various treatment conditions. These treatments were chosen to determine the effects of prochloraz, sodium bicarbonate, ergosterol, aescin and potassium iodide on expression of the analysed defence genes. In addition to the inoculated and non-inoculated cultivar Bohemia, we additionally examined two other varieties of wheat with contrasting resistance to Fusarium sp. infection. These were the blue aleurone layer variety Scorpion that is susceptible to Fusarium sp. infection and variety V2-49-17 with yellow endosperm and partial resistance to Fusarium sp. infection. In this manner, we were able to compare potential effects of inductors upon defence gene expression among three varieties with different susceptibility to infection but also between inoculated and non-inoculated seedlings of a single variety. The lowest infection levels were detected in the sodium bicarbonate treatment. Sodium bicarbonate had not only negative influence on Fusarium growth but also positively affected expression of plant defence genes. Expression of the four marker genes shown to be important in plant defence was significantly affected by the treatments. The greatest upregulation in comparison to the water control was identified under all treatments for the B2H2 gene. Only expression of PAL under the ergosterol and prochloraz treatments were not statistically significant.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32045412</pmid><doi>10.1371/journal.pone.0224413</doi><tpages>e0224413</tpages><orcidid>https://orcid.org/0000-0002-9095-3934</orcidid><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_2353558250 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aleurone layer Analysis Biology and Life Sciences Blight Carbonates Cereals Chitinase Contamination Crop yield Cultivars Disease Resistance - genetics Disease susceptibility Diseases Electronic components Endosperm Ergosterol Experiments Fungi Fusarium Fusarium - immunology Fusarium culmorum Gene expression Gene Expression Profiling Genes Genetic aspects Halides Inductors Infections Iodides Medicine and Health Sciences Mycotoxins Parasites Physical Sciences Phytosterols Plant Diseases - genetics Plant Diseases - immunology Plant Diseases - microbiology Plant genetics Plant lipids Plant pathology Plant sciences Potassium Potassium iodide Potassium iodides Prochloraz Root rot Seed-borne fungi Seedling blight Seedlings Seedlings - genetics Seedlings - microbiology Seeds Sodium Sodium bicarbonate Sodium Bicarbonate - pharmacology Soil contamination Statistical analysis Transcription Transcription (Genetics) Triticum - genetics Water Water control Wheat |
| title | Transcriptional analysis of wheat seedlings inoculated with Fusarium culmorum under continual exposure to disease defence inductors |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T01%3A24%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Transcriptional%20analysis%20of%20wheat%20seedlings%20inoculated%20with%20Fusarium%20culmorum%20under%20continual%20exposure%20to%20disease%20defence%20inductors&rft.jtitle=PloS%20one&rft.au=Antalov%C3%A1,%20Zuzana&rft.date=2020-02-11&rft.volume=15&rft.issue=2&rft.spage=e0224413&rft.epage=e0224413&rft.pages=e0224413-e0224413&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0224413&rft_dat=%3Cgale_plos_%3EA613690638%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2353558250&rft_id=info:pmid/32045412&rft_galeid=A613690638&rft_doaj_id=oai_doaj_org_article_6c9503e941b04d26b6bbb5fa1d60297a&rfr_iscdi=true |