Quantitative PCR provides a simple and accessible method for quantitative microbiota profiling

The use of relative abundance data from next generation sequencing (NGS) can lead to misinterpretations of microbial community structures, as the increase of one taxon leads to the concurrent decrease of the other(s) in compositional data. Although different DNA- and cell-based methods as well as st...

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Veröffentlicht in:	PloS one 2020-01, Vol.15 (1), p.e0227285-e0227285
Hauptverfasser:	Jian, Ching, Luukkonen, Panu, Yki-Järvinen, Hannele, Salonen, Anne, Korpela, Katri
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Biology and Life Sciences Deoxyribonucleic acid DNA DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Feasibility Studies Feces Gene Library High-Throughput Nucleotide Sequencing High-Throughput Screening Assays - methods Humans Intervention Medical research Medicine Microbiomes Microbiota Microbiota - genetics Microorganisms Next-generation sequencing Physical Sciences Polymerase chain reaction Production capacity Profiling Randomized Controlled Trials as Topic Real-Time Polymerase Chain Reaction Relative abundance Research and analysis methods RNA, Ribosomal, 16S - genetics Statistical methods Studies Taxonomy Thermal cycling
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