iTRAQ proteomics reveals the regulatory response to Magnaporthe oryzae in durable resistant vs. susceptible rice genotypes
Rice blast disease caused by Magnaporthe oryzae (M. oryzae) is one of the most serious diseases. Although previous research using two-dimensional gel-based proteomics to assess the proteins related to the rice blast resistance had been done, few proteins were identified. Here, we used the iTRAQ meth...
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| creator | Ma, Zuobin Wang, Lili Zhao, Mingzhu Gu, Shuang Wang, Changhua Zhao, Jiaming Tang, Zhiqiang Gao, Hong Zhang, Liying Fu, Liang Yin, Yongan He, Na Zheng, Wenjing Xu, Zhengjin |
| description | Rice blast disease caused by Magnaporthe oryzae (M. oryzae) is one of the most serious diseases. Although previous research using two-dimensional gel-based proteomics to assess the proteins related to the rice blast resistance had been done, few proteins were identified. Here, we used the iTRAQ method to detect the differentially expressed proteins (DEPs) in the durable resistant rice variety Gangyuan8 (GY8) and the susceptible rice variety Lijiangxintuanheigu (LTH) in response to M. oryzae invasion, and then transcriptome sequencing was used to assist analysis A total of 193 and 672 DEPs were specifically identified in GY8 and LTH, respectively, with only 46 similarly expressed DEPs being shared by GY8 and LTH.39 DEPs involved in plant-pathogen interaction, plant hormone signal transduction, fatty acid metabolism and peroxisome biosynthesis were significantly different between compatible interaction (LTH) and incompatible interaction (GY8). Some proteins participated in peroxide signal transduction and biosynthesis was down-regulated in GY8 but up-regulated in LTH. A lot of genes encoding pathogenesis-related gene (PR), such as chitinase and glucanase, were significantly up-regulated at both the transcriptome and proteome levels at 24 hours post-inoculation in GY8, but up-regulated at the transcriptome level and down-regulated at the proteome level in LTH. Our study reveals that the pathogen-associated molecular pattern (PAMP)-triggered immunity defense system may be activated at the transcriptome level but was inhibited at the protein level in susceptible rice varieties after inoculation. The results may facilitate future studies of the molecular mechanisms of rice blast resistance. |
| doi_str_mv | 10.1371/journal.pone.0227470 |
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Although previous research using two-dimensional gel-based proteomics to assess the proteins related to the rice blast resistance had been done, few proteins were identified. Here, we used the iTRAQ method to detect the differentially expressed proteins (DEPs) in the durable resistant rice variety Gangyuan8 (GY8) and the susceptible rice variety Lijiangxintuanheigu (LTH) in response to M. oryzae invasion, and then transcriptome sequencing was used to assist analysis A total of 193 and 672 DEPs were specifically identified in GY8 and LTH, respectively, with only 46 similarly expressed DEPs being shared by GY8 and LTH.39 DEPs involved in plant-pathogen interaction, plant hormone signal transduction, fatty acid metabolism and peroxisome biosynthesis were significantly different between compatible interaction (LTH) and incompatible interaction (GY8). Some proteins participated in peroxide signal transduction and biosynthesis was down-regulated in GY8 but up-regulated in LTH. A lot of genes encoding pathogenesis-related gene (PR), such as chitinase and glucanase, were significantly up-regulated at both the transcriptome and proteome levels at 24 hours post-inoculation in GY8, but up-regulated at the transcriptome level and down-regulated at the proteome level in LTH. Our study reveals that the pathogen-associated molecular pattern (PAMP)-triggered immunity defense system may be activated at the transcriptome level but was inhibited at the protein level in susceptible rice varieties after inoculation. The results may facilitate future studies of the molecular mechanisms of rice blast resistance.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0227470</identifier><identifier>PMID: 31923921</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agriculture ; Biology and Life Sciences ; Biosynthesis ; Chitinase ; Chromatography, High Pressure Liquid ; Chromatography, Ion Exchange ; Disease ; Disease Resistance - genetics ; Fatty acids ; Gene expression ; Gene Expression Regulation, Plant ; Genotype ; Genotypes ; Host-Pathogen Interactions - genetics ; Inoculation ; Magnaporthe - physiology ; Magnaporthe oryzae ; Metabolism ; Molecular modelling ; Oryza - metabolism ; Oryza - microbiology ; Pathogenesis ; Pathogens ; Peroxide ; Plant Diseases - genetics ; Plant Diseases - microbiology ; Plant hormones ; Plant Proteins - metabolism ; Plant resistance ; Protein Interaction Maps - genetics ; Protein-Serine-Threonine Kinases - metabolism ; Proteins ; Proteome - analysis ; Proteomes ; Proteomics ; Proteomics - methods ; Research and Analysis Methods ; Rice ; Rice blast ; Signal transduction ; Spectrometry, Mass, Electrospray Ionization ; Studies</subject><ispartof>PloS one, 2020-01, Vol.15 (1), p.e0227470-e0227470</ispartof><rights>2020 Ma et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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The results may facilitate future studies of the molecular mechanisms of rice blast resistance.</description><subject>Agriculture</subject><subject>Biology and Life Sciences</subject><subject>Biosynthesis</subject><subject>Chitinase</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chromatography, Ion Exchange</subject><subject>Disease</subject><subject>Disease Resistance - genetics</subject><subject>Fatty acids</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Host-Pathogen Interactions - genetics</subject><subject>Inoculation</subject><subject>Magnaporthe - physiology</subject><subject>Magnaporthe oryzae</subject><subject>Metabolism</subject><subject>Molecular modelling</subject><subject>Oryza - metabolism</subject><subject>Oryza - microbiology</subject><subject>Pathogenesis</subject><subject>Pathogens</subject><subject>Peroxide</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - microbiology</subject><subject>Plant hormones</subject><subject>Plant Proteins - metabolism</subject><subject>Plant resistance</subject><subject>Protein Interaction Maps - genetics</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>Proteins</subject><subject>Proteome - analysis</subject><subject>Proteomes</subject><subject>Proteomics</subject><subject>Proteomics - methods</subject><subject>Research and Analysis Methods</subject><subject>Rice</subject><subject>Rice blast</subject><subject>Signal transduction</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Studies</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUktv1DAQjhCIlsI_QGCpFy67-BU7uSBVFY9KRQhUzpbjjFOvsnGwnZW2vx5nN61axMkjz_eYGX1F8ZbgNWGSfNz4KQy6X49-gDWmVHKJnxWnpGZ0JShmzx_VJ8WrGDcYl6wS4mVxwkhNWU3JaXHnbn5d_ERj8An81pmIAuxA9xGlW8h1N_U6-bDPZcxOEVDy6LvuBj36MENy704DcgNqp6CbfiZFF5MeEtrFNYpTNDAmd-g4A6iDwaf9CPF18cJmI3izvGfF7y-fby6_ra5_fL26vLhemZKKtLJYWtAVkUILbhmWgNvaYlNhwxvDSssJYGNsWTaEYcwIo6I2jbSMWm6rkp0V74-6Y--jWs4WFWWsFBktZUZcHRGt1xs1BrfVYa-8durw4UOndEjO9KA01TWUJbZNS7gkVVVraInhhDeMAqZZ69PiNjVbaA0MKej-iejTzuBuVed3StQlx5JlgQ-LQPB_JohJbV0-Yd_rAfx0mFtQLjCfoef_QP-_HT-iTPAxBrAPwxCs5ijds9QcJbVEKdPePV7kgXSfHfYXnwTJ3w</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>Ma, Zuobin</creator><creator>Wang, Lili</creator><creator>Zhao, Mingzhu</creator><creator>Gu, Shuang</creator><creator>Wang, Changhua</creator><creator>Zhao, Jiaming</creator><creator>Tang, Zhiqiang</creator><creator>Gao, Hong</creator><creator>Zhang, Liying</creator><creator>Fu, Liang</creator><creator>Yin, Yongan</creator><creator>He, Na</creator><creator>Zheng, Wenjing</creator><creator>Xu, Zhengjin</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-8692-7090</orcidid></search><sort><creationdate>20200101</creationdate><title>iTRAQ proteomics reveals the regulatory response to Magnaporthe oryzae in durable resistant vs. susceptible rice genotypes</title><author>Ma, Zuobin ; Wang, Lili ; Zhao, Mingzhu ; Gu, Shuang ; Wang, Changhua ; Zhao, Jiaming ; Tang, Zhiqiang ; Gao, Hong ; Zhang, Liying ; Fu, Liang ; Yin, Yongan ; He, Na ; Zheng, Wenjing ; Xu, Zhengjin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-f07fea8176a64f307e0d9f0c80c4bc35f41e0ccf55b1300313269cb7f32f4f853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Agriculture</topic><topic>Biology and Life Sciences</topic><topic>Biosynthesis</topic><topic>Chitinase</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Chromatography, Ion Exchange</topic><topic>Disease</topic><topic>Disease Resistance - 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Although previous research using two-dimensional gel-based proteomics to assess the proteins related to the rice blast resistance had been done, few proteins were identified. Here, we used the iTRAQ method to detect the differentially expressed proteins (DEPs) in the durable resistant rice variety Gangyuan8 (GY8) and the susceptible rice variety Lijiangxintuanheigu (LTH) in response to M. oryzae invasion, and then transcriptome sequencing was used to assist analysis A total of 193 and 672 DEPs were specifically identified in GY8 and LTH, respectively, with only 46 similarly expressed DEPs being shared by GY8 and LTH.39 DEPs involved in plant-pathogen interaction, plant hormone signal transduction, fatty acid metabolism and peroxisome biosynthesis were significantly different between compatible interaction (LTH) and incompatible interaction (GY8). Some proteins participated in peroxide signal transduction and biosynthesis was down-regulated in GY8 but up-regulated in LTH. A lot of genes encoding pathogenesis-related gene (PR), such as chitinase and glucanase, were significantly up-regulated at both the transcriptome and proteome levels at 24 hours post-inoculation in GY8, but up-regulated at the transcriptome level and down-regulated at the proteome level in LTH. Our study reveals that the pathogen-associated molecular pattern (PAMP)-triggered immunity defense system may be activated at the transcriptome level but was inhibited at the protein level in susceptible rice varieties after inoculation. The results may facilitate future studies of the molecular mechanisms of rice blast resistance.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31923921</pmid><doi>10.1371/journal.pone.0227470</doi><orcidid>https://orcid.org/0000-0001-8692-7090</orcidid><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_2335631377 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Agriculture Biology and Life Sciences Biosynthesis Chitinase Chromatography, High Pressure Liquid Chromatography, Ion Exchange Disease Disease Resistance - genetics Fatty acids Gene expression Gene Expression Regulation, Plant Genotype Genotypes Host-Pathogen Interactions - genetics Inoculation Magnaporthe - physiology Magnaporthe oryzae Metabolism Molecular modelling Oryza - metabolism Oryza - microbiology Pathogenesis Pathogens Peroxide Plant Diseases - genetics Plant Diseases - microbiology Plant hormones Plant Proteins - metabolism Plant resistance Protein Interaction Maps - genetics Protein-Serine-Threonine Kinases - metabolism Proteins Proteome - analysis Proteomes Proteomics Proteomics - methods Research and Analysis Methods Rice Rice blast Signal transduction Spectrometry, Mass, Electrospray Ionization Studies |
| title | iTRAQ proteomics reveals the regulatory response to Magnaporthe oryzae in durable resistant vs. susceptible rice genotypes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T09%3A57%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=iTRAQ%20proteomics%20reveals%20the%20regulatory%20response%20to%20Magnaporthe%20oryzae%20in%20durable%20resistant%20vs.%20susceptible%20rice%20genotypes&rft.jtitle=PloS%20one&rft.au=Ma,%20Zuobin&rft.date=2020-01-01&rft.volume=15&rft.issue=1&rft.spage=e0227470&rft.epage=e0227470&rft.pages=e0227470-e0227470&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0227470&rft_dat=%3Cproquest_plos_%3E2335631377%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2335631377&rft_id=info:pmid/31923921&rft_doaj_id=oai_doaj_org_article_a2a9e550fbd1471889aed1c414b32e02&rfr_iscdi=true |