Use of Nanotrap particles for the capture and enrichment of Zika, chikungunya and dengue viruses in urine
Nanotrap® (NT) particles are hydrogel microspheres developed for target analyte separation and discovery applications. NT particles consist of cross-linked N-isopropylacrylamide (NIPAm) copolymers that are functionalized with a variety of chemical affinity baits to enable broad-spectrum collection a...
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| Veröffentlicht in: | PloS one 2020-01, Vol.15 (1), p.e0227058-e0227058 |
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| creator | Lin, Shih-Chao Carey, Brian D Callahan, Victoria Lee, Ji-Hyun Bracci, Nicole Patnaik, Anurag Smith, Amy K Narayanan, Aarthi Lepene, Benjamin Kehn-Hall, Kylene |
| description | Nanotrap® (NT) particles are hydrogel microspheres developed for target analyte separation and discovery applications. NT particles consist of cross-linked N-isopropylacrylamide (NIPAm) copolymers that are functionalized with a variety of chemical affinity baits to enable broad-spectrum collection and retention of target proteins, nucleic acids, and pathogens. NT particles have been previously shown to capture and enrich arboviruses including Rift Valley fever and Venezuelan equine encephalitis viruses. Yet, there is still a need to enhance the detection ability for other re-emerging viruses such as Zika (ZIKV), chikungunya (CHIKV), and dengue (DENV) viruses. In this study, we exploited NT particles with different affinity baits, including cibacron blue, acrylic acid, and reactive red 120, to evaluate their capturing and enrichment capability for ZIKV, DENV and CHIKV in human fluids. Our results demonstrate that CN1030, a NT particle conjugated with reactive red 120, can recover between 8-16-fold greater genomic copies of ZIKV, CHIKV and DENV in virus spiked urine samples via RT-qPCR, superior to the other chemical baits. Also, we observed that CN1030 simultaneously enriched ZIKV, CHIKV and DENV in co-infection-based settings and could stabilize ZIKV, but not CHIKV infectivity in saliva spiked samples. CN1030 enriched viral detection at various viral concentrations, with significant enhancement observed at viral titers as low as 100 PFU/mL for ZIKV and 10 PFU/mL for CHIKV. The detection of ZIKV was further enhanced with NT particles by processing of larger volume urine samples. Furthermore, we developed a magnetic NT particle, CN3080, based on the same backbone of CN1030, and demonstrated that CN3080 could also capture and enrich ZIKV and CHIKV in a dose-dependent manner. Finally, in silico docking predictions support that the affinity between reactive red 120 and ZIKV or CHIKV envelope proteins appeared to be greater than acrylic acid. Overall, our data show that NT particles along with reactive red 120 can be utilized as a pre-processing technology for enhancement of detecting febrile-illness causing viruses. |
| doi_str_mv | 10.1371/journal.pone.0227058 |
| format | Article |
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NT particles consist of cross-linked N-isopropylacrylamide (NIPAm) copolymers that are functionalized with a variety of chemical affinity baits to enable broad-spectrum collection and retention of target proteins, nucleic acids, and pathogens. NT particles have been previously shown to capture and enrich arboviruses including Rift Valley fever and Venezuelan equine encephalitis viruses. Yet, there is still a need to enhance the detection ability for other re-emerging viruses such as Zika (ZIKV), chikungunya (CHIKV), and dengue (DENV) viruses. In this study, we exploited NT particles with different affinity baits, including cibacron blue, acrylic acid, and reactive red 120, to evaluate their capturing and enrichment capability for ZIKV, DENV and CHIKV in human fluids. Our results demonstrate that CN1030, a NT particle conjugated with reactive red 120, can recover between 8-16-fold greater genomic copies of ZIKV, CHIKV and DENV in virus spiked urine samples via RT-qPCR, superior to the other chemical baits. Also, we observed that CN1030 simultaneously enriched ZIKV, CHIKV and DENV in co-infection-based settings and could stabilize ZIKV, but not CHIKV infectivity in saliva spiked samples. CN1030 enriched viral detection at various viral concentrations, with significant enhancement observed at viral titers as low as 100 PFU/mL for ZIKV and 10 PFU/mL for CHIKV. The detection of ZIKV was further enhanced with NT particles by processing of larger volume urine samples. Furthermore, we developed a magnetic NT particle, CN3080, based on the same backbone of CN1030, and demonstrated that CN3080 could also capture and enrich ZIKV and CHIKV in a dose-dependent manner. Finally, in silico docking predictions support that the affinity between reactive red 120 and ZIKV or CHIKV envelope proteins appeared to be greater than acrylic acid. Overall, our data show that NT particles along with reactive red 120 can be utilized as a pre-processing technology for enhancement of detecting febrile-illness causing viruses.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0227058</identifier><identifier>PMID: 31910225</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Acrylic acid ; Affinity ; Arbovirus Infections - diagnosis ; Arbovirus Infections - urine ; Arbovirus Infections - virology ; Baits ; Biology ; Biology and life sciences ; Chikungunya virus ; Chikungunya virus - genetics ; Chikungunya virus - isolation & purification ; Chikungunya virus - pathogenicity ; Coccidioidomycosis ; Coloring Agents - chemistry ; Copolymers ; Crosslinking ; Dengue ; Dengue fever ; Dengue virus ; Dengue Virus - genetics ; Dengue Virus - isolation & purification ; Dengue Virus - pathogenicity ; Encephalitis ; Enrichment ; Equine encephalomyelitis ; Experiments ; Humans ; Hydrogels ; Hydrogels - chemistry ; Infections ; Infectious diseases ; Infectivity ; Isopropylacrylamide ; Medicine and Health Sciences ; Methods ; Microspheres ; Molecular Diagnostic Techniques - methods ; Nanoparticles - chemistry ; Nanoparticles - metabolism ; Nucleic acids ; Organic chemistry ; Pathogenic microorganisms ; Pathogens ; Physical Sciences ; Polymerase Chain Reaction - methods ; Protein Binding ; Proteins ; Rift Valley fever ; Saliva ; Saliva - virology ; Technology ; Technology application ; Urine ; Urine - virology ; Vector-borne diseases ; Venezuelan equine encephalitis ; Viral diseases ; Viral Envelope Proteins - chemistry ; Viral Envelope Proteins - metabolism ; Viruses ; Zika virus ; Zika Virus - genetics ; Zika Virus - isolation & purification ; Zika Virus - pathogenicity</subject><ispartof>PloS one, 2020-01, Vol.15 (1), p.e0227058-e0227058</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Lin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Lin et al 2020 Lin et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-ed5b9f8389fbb6943a0d413a90acb0131d4ba8ce00149501688712ed44ef23a3</citedby><cites>FETCH-LOGICAL-c692t-ed5b9f8389fbb6943a0d413a90acb0131d4ba8ce00149501688712ed44ef23a3</cites><orcidid>0000-0003-2942-5937 ; 0000-0002-4576-3998 ; 0000-0001-8036-7213</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946132/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946132/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31910225$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Shih-Chao</creatorcontrib><creatorcontrib>Carey, Brian D</creatorcontrib><creatorcontrib>Callahan, Victoria</creatorcontrib><creatorcontrib>Lee, Ji-Hyun</creatorcontrib><creatorcontrib>Bracci, Nicole</creatorcontrib><creatorcontrib>Patnaik, Anurag</creatorcontrib><creatorcontrib>Smith, Amy K</creatorcontrib><creatorcontrib>Narayanan, Aarthi</creatorcontrib><creatorcontrib>Lepene, Benjamin</creatorcontrib><creatorcontrib>Kehn-Hall, Kylene</creatorcontrib><title>Use of Nanotrap particles for the capture and enrichment of Zika, chikungunya and dengue viruses in urine</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Nanotrap® (NT) particles are hydrogel microspheres developed for target analyte separation and discovery applications. NT particles consist of cross-linked N-isopropylacrylamide (NIPAm) copolymers that are functionalized with a variety of chemical affinity baits to enable broad-spectrum collection and retention of target proteins, nucleic acids, and pathogens. NT particles have been previously shown to capture and enrich arboviruses including Rift Valley fever and Venezuelan equine encephalitis viruses. Yet, there is still a need to enhance the detection ability for other re-emerging viruses such as Zika (ZIKV), chikungunya (CHIKV), and dengue (DENV) viruses. In this study, we exploited NT particles with different affinity baits, including cibacron blue, acrylic acid, and reactive red 120, to evaluate their capturing and enrichment capability for ZIKV, DENV and CHIKV in human fluids. Our results demonstrate that CN1030, a NT particle conjugated with reactive red 120, can recover between 8-16-fold greater genomic copies of ZIKV, CHIKV and DENV in virus spiked urine samples via RT-qPCR, superior to the other chemical baits. Also, we observed that CN1030 simultaneously enriched ZIKV, CHIKV and DENV in co-infection-based settings and could stabilize ZIKV, but not CHIKV infectivity in saliva spiked samples. CN1030 enriched viral detection at various viral concentrations, with significant enhancement observed at viral titers as low as 100 PFU/mL for ZIKV and 10 PFU/mL for CHIKV. The detection of ZIKV was further enhanced with NT particles by processing of larger volume urine samples. Furthermore, we developed a magnetic NT particle, CN3080, based on the same backbone of CN1030, and demonstrated that CN3080 could also capture and enrich ZIKV and CHIKV in a dose-dependent manner. Finally, in silico docking predictions support that the affinity between reactive red 120 and ZIKV or CHIKV envelope proteins appeared to be greater than acrylic acid. Overall, our data show that NT particles along with reactive red 120 can be utilized as a pre-processing technology for enhancement of detecting febrile-illness causing viruses.</description><subject>Acids</subject><subject>Acrylic acid</subject><subject>Affinity</subject><subject>Arbovirus Infections - diagnosis</subject><subject>Arbovirus Infections - urine</subject><subject>Arbovirus Infections - virology</subject><subject>Baits</subject><subject>Biology</subject><subject>Biology and life sciences</subject><subject>Chikungunya virus</subject><subject>Chikungunya virus - genetics</subject><subject>Chikungunya virus - isolation & purification</subject><subject>Chikungunya virus - pathogenicity</subject><subject>Coccidioidomycosis</subject><subject>Coloring Agents - chemistry</subject><subject>Copolymers</subject><subject>Crosslinking</subject><subject>Dengue</subject><subject>Dengue fever</subject><subject>Dengue virus</subject><subject>Dengue Virus - genetics</subject><subject>Dengue Virus - isolation & purification</subject><subject>Dengue Virus - pathogenicity</subject><subject>Encephalitis</subject><subject>Enrichment</subject><subject>Equine encephalomyelitis</subject><subject>Experiments</subject><subject>Humans</subject><subject>Hydrogels</subject><subject>Hydrogels - chemistry</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Infectivity</subject><subject>Isopropylacrylamide</subject><subject>Medicine and Health Sciences</subject><subject>Methods</subject><subject>Microspheres</subject><subject>Molecular Diagnostic Techniques - methods</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - metabolism</subject><subject>Nucleic acids</subject><subject>Organic chemistry</subject><subject>Pathogenic microorganisms</subject><subject>Pathogens</subject><subject>Physical Sciences</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Protein Binding</subject><subject>Proteins</subject><subject>Rift Valley fever</subject><subject>Saliva</subject><subject>Saliva - virology</subject><subject>Technology</subject><subject>Technology application</subject><subject>Urine</subject><subject>Urine - virology</subject><subject>Vector-borne diseases</subject><subject>Venezuelan equine encephalitis</subject><subject>Viral diseases</subject><subject>Viral Envelope Proteins - chemistry</subject><subject>Viral Envelope Proteins - metabolism</subject><subject>Viruses</subject><subject>Zika virus</subject><subject>Zika Virus - genetics</subject><subject>Zika Virus - isolation & purification</subject><subject>Zika Virus - pathogenicity</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1-PlDAUxYnRuOvoNzBKYmI0ccaWQikvJpuNfybZuImuPvjSlPZ26CzTYgsb99tbdtjNYPZBeADK7xzuPeUmyXOMVpiU-P3WDd6KdtU5CyuUZSUq2IPkGFckW9IMkYcH90fJkxC2CBWEUfo4OSK4wlFSHCfmR4DU6fSrsK73oks74XsjWwipdj7tG0il6PrBQyqsSsF6I5sd2H4U_TKX4l0qG3M52M1gr8UNoyA-QHpl_BCijbHp4I2Fp8kjLdoAz6brIrn49PHi9Mvy7Pzz-vTkbClplfVLUEVdaUZYpeuaVjkRSOWYiAoJWSNMsMprwSQghPOqQJgyVuIMVJ6Dzoggi-Tl3rZrXeBTSIFnhOSYlYiVkVjvCeXElnfe7IS_5k4YfrPg_IZPGXBVYK2BxEPTHBUFUwxqKglhCjIRq1kkH6avDfUOlIzBeNHOTOdvrGn4xl3x2BnFJIsGbyYD734PEHq-M0FC2woLbtjXXSJC6Vj3q3_Q-7ubqI2IDRirx22Voyk_oRiVhOA8j9TqHiqeCnZGxj9Km7g-E7ydCSLTw59-I4YQ-Pr7t_9nz3_O2dcHbAOi7Zvg2qE3zoY5mO9B6V0IHvRdyBjxcSBu0-DjQPBpIKLsxeEG3YluJ4D8BeCLBSo</recordid><startdate>20200107</startdate><enddate>20200107</enddate><creator>Lin, Shih-Chao</creator><creator>Carey, Brian D</creator><creator>Callahan, Victoria</creator><creator>Lee, Ji-Hyun</creator><creator>Bracci, Nicole</creator><creator>Patnaik, Anurag</creator><creator>Smith, Amy K</creator><creator>Narayanan, Aarthi</creator><creator>Lepene, Benjamin</creator><creator>Kehn-Hall, Kylene</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-2942-5937</orcidid><orcidid>https://orcid.org/0000-0002-4576-3998</orcidid><orcidid>https://orcid.org/0000-0001-8036-7213</orcidid></search><sort><creationdate>20200107</creationdate><title>Use of Nanotrap particles for the capture and enrichment of Zika, chikungunya and dengue viruses in urine</title><author>Lin, Shih-Chao ; Carey, Brian D ; Callahan, Victoria ; Lee, Ji-Hyun ; Bracci, Nicole ; Patnaik, Anurag ; Smith, Amy K ; Narayanan, Aarthi ; Lepene, Benjamin ; Kehn-Hall, Kylene</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-ed5b9f8389fbb6943a0d413a90acb0131d4ba8ce00149501688712ed44ef23a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acids</topic><topic>Acrylic acid</topic><topic>Affinity</topic><topic>Arbovirus Infections - diagnosis</topic><topic>Arbovirus Infections - urine</topic><topic>Arbovirus Infections - virology</topic><topic>Baits</topic><topic>Biology</topic><topic>Biology and life sciences</topic><topic>Chikungunya virus</topic><topic>Chikungunya virus - genetics</topic><topic>Chikungunya virus - isolation & purification</topic><topic>Chikungunya virus - pathogenicity</topic><topic>Coccidioidomycosis</topic><topic>Coloring Agents - chemistry</topic><topic>Copolymers</topic><topic>Crosslinking</topic><topic>Dengue</topic><topic>Dengue fever</topic><topic>Dengue virus</topic><topic>Dengue Virus - genetics</topic><topic>Dengue Virus - isolation & purification</topic><topic>Dengue Virus - pathogenicity</topic><topic>Encephalitis</topic><topic>Enrichment</topic><topic>Equine encephalomyelitis</topic><topic>Experiments</topic><topic>Humans</topic><topic>Hydrogels</topic><topic>Hydrogels - chemistry</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Infectivity</topic><topic>Isopropylacrylamide</topic><topic>Medicine and Health Sciences</topic><topic>Methods</topic><topic>Microspheres</topic><topic>Molecular Diagnostic Techniques - methods</topic><topic>Nanoparticles - chemistry</topic><topic>Nanoparticles - metabolism</topic><topic>Nucleic acids</topic><topic>Organic chemistry</topic><topic>Pathogenic microorganisms</topic><topic>Pathogens</topic><topic>Physical Sciences</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Protein Binding</topic><topic>Proteins</topic><topic>Rift Valley fever</topic><topic>Saliva</topic><topic>Saliva - virology</topic><topic>Technology</topic><topic>Technology application</topic><topic>Urine</topic><topic>Urine - virology</topic><topic>Vector-borne diseases</topic><topic>Venezuelan equine encephalitis</topic><topic>Viral diseases</topic><topic>Viral Envelope Proteins - chemistry</topic><topic>Viral Envelope Proteins - metabolism</topic><topic>Viruses</topic><topic>Zika virus</topic><topic>Zika Virus - genetics</topic><topic>Zika Virus - isolation & purification</topic><topic>Zika Virus - pathogenicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Shih-Chao</creatorcontrib><creatorcontrib>Carey, Brian D</creatorcontrib><creatorcontrib>Callahan, Victoria</creatorcontrib><creatorcontrib>Lee, Ji-Hyun</creatorcontrib><creatorcontrib>Bracci, Nicole</creatorcontrib><creatorcontrib>Patnaik, Anurag</creatorcontrib><creatorcontrib>Smith, Amy K</creatorcontrib><creatorcontrib>Narayanan, Aarthi</creatorcontrib><creatorcontrib>Lepene, Benjamin</creatorcontrib><creatorcontrib>Kehn-Hall, Kylene</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Shih-Chao</au><au>Carey, Brian D</au><au>Callahan, Victoria</au><au>Lee, Ji-Hyun</au><au>Bracci, Nicole</au><au>Patnaik, Anurag</au><au>Smith, Amy K</au><au>Narayanan, Aarthi</au><au>Lepene, Benjamin</au><au>Kehn-Hall, Kylene</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of Nanotrap particles for the capture and enrichment of Zika, chikungunya and dengue viruses in urine</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2020-01-07</date><risdate>2020</risdate><volume>15</volume><issue>1</issue><spage>e0227058</spage><epage>e0227058</epage><pages>e0227058-e0227058</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Nanotrap® (NT) particles are hydrogel microspheres developed for target analyte separation and discovery applications. NT particles consist of cross-linked N-isopropylacrylamide (NIPAm) copolymers that are functionalized with a variety of chemical affinity baits to enable broad-spectrum collection and retention of target proteins, nucleic acids, and pathogens. NT particles have been previously shown to capture and enrich arboviruses including Rift Valley fever and Venezuelan equine encephalitis viruses. Yet, there is still a need to enhance the detection ability for other re-emerging viruses such as Zika (ZIKV), chikungunya (CHIKV), and dengue (DENV) viruses. In this study, we exploited NT particles with different affinity baits, including cibacron blue, acrylic acid, and reactive red 120, to evaluate their capturing and enrichment capability for ZIKV, DENV and CHIKV in human fluids. Our results demonstrate that CN1030, a NT particle conjugated with reactive red 120, can recover between 8-16-fold greater genomic copies of ZIKV, CHIKV and DENV in virus spiked urine samples via RT-qPCR, superior to the other chemical baits. Also, we observed that CN1030 simultaneously enriched ZIKV, CHIKV and DENV in co-infection-based settings and could stabilize ZIKV, but not CHIKV infectivity in saliva spiked samples. CN1030 enriched viral detection at various viral concentrations, with significant enhancement observed at viral titers as low as 100 PFU/mL for ZIKV and 10 PFU/mL for CHIKV. The detection of ZIKV was further enhanced with NT particles by processing of larger volume urine samples. Furthermore, we developed a magnetic NT particle, CN3080, based on the same backbone of CN1030, and demonstrated that CN3080 could also capture and enrich ZIKV and CHIKV in a dose-dependent manner. Finally, in silico docking predictions support that the affinity between reactive red 120 and ZIKV or CHIKV envelope proteins appeared to be greater than acrylic acid. Overall, our data show that NT particles along with reactive red 120 can be utilized as a pre-processing technology for enhancement of detecting febrile-illness causing viruses.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31910225</pmid><doi>10.1371/journal.pone.0227058</doi><tpages>e0227058</tpages><orcidid>https://orcid.org/0000-0003-2942-5937</orcidid><orcidid>https://orcid.org/0000-0002-4576-3998</orcidid><orcidid>https://orcid.org/0000-0001-8036-7213</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2020-01, Vol.15 (1), p.e0227058-e0227058 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2334187087 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acids Acrylic acid Affinity Arbovirus Infections - diagnosis Arbovirus Infections - urine Arbovirus Infections - virology Baits Biology Biology and life sciences Chikungunya virus Chikungunya virus - genetics Chikungunya virus - isolation & purification Chikungunya virus - pathogenicity Coccidioidomycosis Coloring Agents - chemistry Copolymers Crosslinking Dengue Dengue fever Dengue virus Dengue Virus - genetics Dengue Virus - isolation & purification Dengue Virus - pathogenicity Encephalitis Enrichment Equine encephalomyelitis Experiments Humans Hydrogels Hydrogels - chemistry Infections Infectious diseases Infectivity Isopropylacrylamide Medicine and Health Sciences Methods Microspheres Molecular Diagnostic Techniques - methods Nanoparticles - chemistry Nanoparticles - metabolism Nucleic acids Organic chemistry Pathogenic microorganisms Pathogens Physical Sciences Polymerase Chain Reaction - methods Protein Binding Proteins Rift Valley fever Saliva Saliva - virology Technology Technology application Urine Urine - virology Vector-borne diseases Venezuelan equine encephalitis Viral diseases Viral Envelope Proteins - chemistry Viral Envelope Proteins - metabolism Viruses Zika virus Zika Virus - genetics Zika Virus - isolation & purification Zika Virus - pathogenicity |
| title | Use of Nanotrap particles for the capture and enrichment of Zika, chikungunya and dengue viruses in urine |
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