Divulging diazotrophic bacterial community structure in Kuwait desert ecosystems and their N2-fixation potential
Kuwait is a semi-arid region with soils that are relatively nitrogen-poor. Thus, biological nitrogen fixation is an important natural process in which N2-fixing bacteria (diazotrophs) convert atmospheric nitrogen into plant-usable forms such as ammonium and nitrate. Currently, there is limited infor...
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| creator | Suleiman, M K Quoreshi, A M Bhat, N R Manuvel, A J Sivadasan, M T |
| description | Kuwait is a semi-arid region with soils that are relatively nitrogen-poor. Thus, biological nitrogen fixation is an important natural process in which N2-fixing bacteria (diazotrophs) convert atmospheric nitrogen into plant-usable forms such as ammonium and nitrate. Currently, there is limited information on free-living and root-associated nitrogen-fixing bacteria and their potential to fix nitrogen and aid natural plant communities in the Kuwait desert. In this study, free living N2-fixing diazotrophs were enriched and isolated from the rhizosphere soil associated with three native keystone plant species; Rhanterium epapposum, Farsetia aegyptia, and Haloxylon salicornicum. Root-associated bacteria were isolated from the root nodules of Vachellia pachyceras. The result showed that the strains were clustered in five groups represented by class: γ-proteobacteria, and α-proteobacteria; phyla: Actinobacteria being the most dominant, followed by phyla: Firmicutes, and class: β-proteobacteria. This study initially identified 50 nitrogen-fixers by16S rRNA gene sequencing, of which 78% were confirmed to be nitrogen-fixers using the acetylene reduction assay. Among the nitrogen fixers identified, the genus Rhizobium was predominant in the rhizosphere soil of R. epapposum and H. salicornicum, whereas Pseudomonas was predominant in the rhizosphere soil of F. aegyptia, The species Agrobacterium tumefaciens was mainly found to be dominant among the root nodules of V. pachyceras and followed by Cellulomonas, Bacillus, and Pseudomonas genera as root-associated bacteria. The variety of diazotrophs revealed in this study, signifying the enormous importance of free-living and root-associated bacteria in extreme conditions and suggesting potential ecological importance of diazotrophs in arid ecosystem. To our knowledge, this study is the first to use culture-based isolation, molecular identification, and evaluation of N2-fixing ability to detail diazotroph diversity in Kuwaiti desert soils. |
| doi_str_mv | 10.1371/journal.pone.0220679 |
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Thus, biological nitrogen fixation is an important natural process in which N2-fixing bacteria (diazotrophs) convert atmospheric nitrogen into plant-usable forms such as ammonium and nitrate. Currently, there is limited information on free-living and root-associated nitrogen-fixing bacteria and their potential to fix nitrogen and aid natural plant communities in the Kuwait desert. In this study, free living N2-fixing diazotrophs were enriched and isolated from the rhizosphere soil associated with three native keystone plant species; Rhanterium epapposum, Farsetia aegyptia, and Haloxylon salicornicum. Root-associated bacteria were isolated from the root nodules of Vachellia pachyceras. The result showed that the strains were clustered in five groups represented by class: γ-proteobacteria, and α-proteobacteria; phyla: Actinobacteria being the most dominant, followed by phyla: Firmicutes, and class: β-proteobacteria. This study initially identified 50 nitrogen-fixers by16S rRNA gene sequencing, of which 78% were confirmed to be nitrogen-fixers using the acetylene reduction assay. Among the nitrogen fixers identified, the genus Rhizobium was predominant in the rhizosphere soil of R. epapposum and H. salicornicum, whereas Pseudomonas was predominant in the rhizosphere soil of F. aegyptia, The species Agrobacterium tumefaciens was mainly found to be dominant among the root nodules of V. pachyceras and followed by Cellulomonas, Bacillus, and Pseudomonas genera as root-associated bacteria. The variety of diazotrophs revealed in this study, signifying the enormous importance of free-living and root-associated bacteria in extreme conditions and suggesting potential ecological importance of diazotrophs in arid ecosystem. To our knowledge, this study is the first to use culture-based isolation, molecular identification, and evaluation of N2-fixing ability to detail diazotroph diversity in Kuwaiti desert soils.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0220679</identifier><identifier>PMID: 31877136</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acetylene ; Acetylene reduction ; Actinobacteria - genetics ; Ammonia ; Ammonium ; Arid regions ; Arid zones ; Bacteria ; Bacteria - genetics ; Biology and Life Sciences ; Cell culture ; Community structure ; Desert Climate ; Desert soils ; Deserts ; DNA, Ribosomal - genetics ; Ecology and Environmental Sciences ; Ecosystem ; Flowers & plants ; Gene sequencing ; Indigenous species ; Kuwait ; Life sciences ; Microbiota - genetics ; Microorganisms ; Nitrogen ; Nitrogen - metabolism ; Nitrogen fixation ; Nitrogen Fixation - physiology ; Nitrogen-fixing bacteria ; Nitrogenation ; Nodules ; Nutrients ; Phylogeny ; Physical Sciences ; Plant communities ; Plant growth ; Plant populations ; Pseudomonas ; Rhizosphere ; RNA, Ribosomal, 16S - genetics ; Root nodules ; rRNA ; Sandy soils ; Semi arid areas ; Semiarid lands ; Semiarid zones ; Soil - chemistry ; Soil Microbiology ; Terrestrial ecosystems ; Yeast</subject><ispartof>PloS one, 2019-12, Vol.14 (12), p.e0220679-e0220679</ispartof><rights>2019 Suleiman et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Suleiman et al 2019 Suleiman et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-fcf431f3232d0a8c55ac5d1a462b31a6e4095ec6c78734b475d45162fbb36eec3</citedby><cites>FETCH-LOGICAL-c526t-fcf431f3232d0a8c55ac5d1a462b31a6e4095ec6c78734b475d45162fbb36eec3</cites><orcidid>0000-0001-9060-5457 ; 0000-0002-4128-1553</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6932743/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6932743/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31877136$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Zimmerman, Andrew R.</contributor><creatorcontrib>Suleiman, M K</creatorcontrib><creatorcontrib>Quoreshi, A M</creatorcontrib><creatorcontrib>Bhat, N R</creatorcontrib><creatorcontrib>Manuvel, A J</creatorcontrib><creatorcontrib>Sivadasan, M T</creatorcontrib><title>Divulging diazotrophic bacterial community structure in Kuwait desert ecosystems and their N2-fixation potential</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Kuwait is a semi-arid region with soils that are relatively nitrogen-poor. Thus, biological nitrogen fixation is an important natural process in which N2-fixing bacteria (diazotrophs) convert atmospheric nitrogen into plant-usable forms such as ammonium and nitrate. Currently, there is limited information on free-living and root-associated nitrogen-fixing bacteria and their potential to fix nitrogen and aid natural plant communities in the Kuwait desert. In this study, free living N2-fixing diazotrophs were enriched and isolated from the rhizosphere soil associated with three native keystone plant species; Rhanterium epapposum, Farsetia aegyptia, and Haloxylon salicornicum. Root-associated bacteria were isolated from the root nodules of Vachellia pachyceras. The result showed that the strains were clustered in five groups represented by class: γ-proteobacteria, and α-proteobacteria; phyla: Actinobacteria being the most dominant, followed by phyla: Firmicutes, and class: β-proteobacteria. This study initially identified 50 nitrogen-fixers by16S rRNA gene sequencing, of which 78% were confirmed to be nitrogen-fixers using the acetylene reduction assay. Among the nitrogen fixers identified, the genus Rhizobium was predominant in the rhizosphere soil of R. epapposum and H. salicornicum, whereas Pseudomonas was predominant in the rhizosphere soil of F. aegyptia, The species Agrobacterium tumefaciens was mainly found to be dominant among the root nodules of V. pachyceras and followed by Cellulomonas, Bacillus, and Pseudomonas genera as root-associated bacteria. The variety of diazotrophs revealed in this study, signifying the enormous importance of free-living and root-associated bacteria in extreme conditions and suggesting potential ecological importance of diazotrophs in arid ecosystem. To our knowledge, this study is the first to use culture-based isolation, molecular identification, and evaluation of N2-fixing ability to detail diazotroph diversity in Kuwaiti desert soils.</description><subject>Acetylene</subject><subject>Acetylene reduction</subject><subject>Actinobacteria - genetics</subject><subject>Ammonia</subject><subject>Ammonium</subject><subject>Arid regions</subject><subject>Arid zones</subject><subject>Bacteria</subject><subject>Bacteria - genetics</subject><subject>Biology and Life Sciences</subject><subject>Cell culture</subject><subject>Community structure</subject><subject>Desert Climate</subject><subject>Desert soils</subject><subject>Deserts</subject><subject>DNA, Ribosomal - genetics</subject><subject>Ecology and Environmental Sciences</subject><subject>Ecosystem</subject><subject>Flowers & plants</subject><subject>Gene sequencing</subject><subject>Indigenous species</subject><subject>Kuwait</subject><subject>Life sciences</subject><subject>Microbiota - 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Thus, biological nitrogen fixation is an important natural process in which N2-fixing bacteria (diazotrophs) convert atmospheric nitrogen into plant-usable forms such as ammonium and nitrate. Currently, there is limited information on free-living and root-associated nitrogen-fixing bacteria and their potential to fix nitrogen and aid natural plant communities in the Kuwait desert. In this study, free living N2-fixing diazotrophs were enriched and isolated from the rhizosphere soil associated with three native keystone plant species; Rhanterium epapposum, Farsetia aegyptia, and Haloxylon salicornicum. Root-associated bacteria were isolated from the root nodules of Vachellia pachyceras. The result showed that the strains were clustered in five groups represented by class: γ-proteobacteria, and α-proteobacteria; phyla: Actinobacteria being the most dominant, followed by phyla: Firmicutes, and class: β-proteobacteria. This study initially identified 50 nitrogen-fixers by16S rRNA gene sequencing, of which 78% were confirmed to be nitrogen-fixers using the acetylene reduction assay. Among the nitrogen fixers identified, the genus Rhizobium was predominant in the rhizosphere soil of R. epapposum and H. salicornicum, whereas Pseudomonas was predominant in the rhizosphere soil of F. aegyptia, The species Agrobacterium tumefaciens was mainly found to be dominant among the root nodules of V. pachyceras and followed by Cellulomonas, Bacillus, and Pseudomonas genera as root-associated bacteria. The variety of diazotrophs revealed in this study, signifying the enormous importance of free-living and root-associated bacteria in extreme conditions and suggesting potential ecological importance of diazotrophs in arid ecosystem. To our knowledge, this study is the first to use culture-based isolation, molecular identification, and evaluation of N2-fixing ability to detail diazotroph diversity in Kuwaiti desert soils.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31877136</pmid><doi>10.1371/journal.pone.0220679</doi><orcidid>https://orcid.org/0000-0001-9060-5457</orcidid><orcidid>https://orcid.org/0000-0002-4128-1553</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2019-12, Vol.14 (12), p.e0220679-e0220679 |
| issn | 1932-6203 1932-6203 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acetylene Acetylene reduction Actinobacteria - genetics Ammonia Ammonium Arid regions Arid zones Bacteria Bacteria - genetics Biology and Life Sciences Cell culture Community structure Desert Climate Desert soils Deserts DNA, Ribosomal - genetics Ecology and Environmental Sciences Ecosystem Flowers & plants Gene sequencing Indigenous species Kuwait Life sciences Microbiota - genetics Microorganisms Nitrogen Nitrogen - metabolism Nitrogen fixation Nitrogen Fixation - physiology Nitrogen-fixing bacteria Nitrogenation Nodules Nutrients Phylogeny Physical Sciences Plant communities Plant growth Plant populations Pseudomonas Rhizosphere RNA, Ribosomal, 16S - genetics Root nodules rRNA Sandy soils Semi arid areas Semiarid lands Semiarid zones Soil - chemistry Soil Microbiology Terrestrial ecosystems Yeast |
| title | Divulging diazotrophic bacterial community structure in Kuwait desert ecosystems and their N2-fixation potential |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T11%3A11%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Divulging%20diazotrophic%20bacterial%20community%20structure%20in%20Kuwait%20desert%20ecosystems%20and%20their%20N2-fixation%20potential&rft.jtitle=PloS%20one&rft.au=Suleiman,%20M%20K&rft.date=2019-12-26&rft.volume=14&rft.issue=12&rft.spage=e0220679&rft.epage=e0220679&rft.pages=e0220679-e0220679&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0220679&rft_dat=%3Cproquest_plos_%3E2330775377%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2330775377&rft_id=info:pmid/31877136&rft_doaj_id=oai_doaj_org_article_9f7c3d3cdaf54b308c8bba6f736b12b2&rfr_iscdi=true |