Effect of tracheal antimicrobial peptide on the development of Mannheimia haemolytica pneumonia in cattle
Bacterial pneumonia causes significant economic loss to the beef industry and occurs at times of stress and viral infection. Administering antibiotics to at-risk calves is often used to prevent the disease, but alternatives to mass treatment with antibiotics are needed. Tracheal antimicrobial peptid...
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| creator | Vulikh, Ksenia Bassel, Laura L Sergejewich, Lauren Kaufman, Emily I Hewson, Joanne MacInnes, Janet I Tabatabaei, Saeid Caswell, Jeff L |
| description | Bacterial pneumonia causes significant economic loss to the beef industry and occurs at times of stress and viral infection. Administering antibiotics to at-risk calves is often used to prevent the disease, but alternatives to mass treatment with antibiotics are needed. Tracheal antimicrobial peptide (TAP), a β-defensin naturally produced by bovine airways, has bactericidal activity against the pathogens that cause pneumonia in cattle. However, TAP expression is suppressed by glucocorticoid (stress) and viral infection. We hypothesized that delivering TAP to the respiratory tract would prevent development of pneumonia in calves infected with Mannheimia haemolytica. Clean-catch calves (i.e. obtained prior to contact with the dam) were challenged by aerosol with M. haemolytica, and TAP or water was delivered to the respiratory tract at 0.3, 2 and 6 hours post-infection. TAP treatment did not protect against development of disease. Calves treated with TAP had similar bacterial loads in the nasal cavity and lung compared to calves treated with water. Similarly, TAP treatment did not affect the development of clinical signs, elevated rectal temperatures, or increased levels of blood neutrophils, haptoglobin and fibrinogen that occurred after bacterial challenge. Postmortem gross and histologic lung lesions were also similar in the two groups. To determine why there was a lack of protective effect, we tested the effect of substances in respiratory lining fluid on the bactericidal activity of TAP. Physiologic concentrations of sodium chloride inhibited TAP bactericidal activity in vitro, as did serum at concentrations of 0.62 to 2.5%, but concentrated bronchoalveolar lavage fluid had no consistent effect. These findings suggest that TAP does not have in vivo bactericidal activity against M. haemolytica because of interference by physiological sodium chloride levels and by serum. Thus, administration of TAP may not be effective for prevention of M. haemolytica pneumonia. |
| doi_str_mv | 10.1371/journal.pone.0225533 |
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Administering antibiotics to at-risk calves is often used to prevent the disease, but alternatives to mass treatment with antibiotics are needed. Tracheal antimicrobial peptide (TAP), a β-defensin naturally produced by bovine airways, has bactericidal activity against the pathogens that cause pneumonia in cattle. However, TAP expression is suppressed by glucocorticoid (stress) and viral infection. We hypothesized that delivering TAP to the respiratory tract would prevent development of pneumonia in calves infected with Mannheimia haemolytica. Clean-catch calves (i.e. obtained prior to contact with the dam) were challenged by aerosol with M. haemolytica, and TAP or water was delivered to the respiratory tract at 0.3, 2 and 6 hours post-infection. TAP treatment did not protect against development of disease. Calves treated with TAP had similar bacterial loads in the nasal cavity and lung compared to calves treated with water. Similarly, TAP treatment did not affect the development of clinical signs, elevated rectal temperatures, or increased levels of blood neutrophils, haptoglobin and fibrinogen that occurred after bacterial challenge. Postmortem gross and histologic lung lesions were also similar in the two groups. To determine why there was a lack of protective effect, we tested the effect of substances in respiratory lining fluid on the bactericidal activity of TAP. Physiologic concentrations of sodium chloride inhibited TAP bactericidal activity in vitro, as did serum at concentrations of 0.62 to 2.5%, but concentrated bronchoalveolar lavage fluid had no consistent effect. These findings suggest that TAP does not have in vivo bactericidal activity against M. haemolytica because of interference by physiological sodium chloride levels and by serum. Thus, administration of TAP may not be effective for prevention of M. haemolytica pneumonia.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0225533</identifier><identifier>PMID: 31770402</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Alveoli ; Animals ; Anti-Infective Agents - chemistry ; Anti-Infective Agents - pharmacology ; Anti-Infective Agents - therapeutic use ; Antibiotics ; Antiinfectives and antibacterials ; Antimicrobial agents ; Antimicrobial Cationic Peptides - chemistry ; Antimicrobial Cationic Peptides - pharmacology ; Antimicrobial Cationic Peptides - therapeutic use ; Antimicrobial peptides ; Autopsy ; Bacteria ; Bacterial infections ; Bactericidal activity ; Beef ; Beef cattle ; Beef industry ; Biology and Life Sciences ; Bronchoalveolar lavage ; Bronchoalveolar Lavage Fluid - microbiology ; Bronchus ; Calves ; Cattle ; Cattle Diseases - drug therapy ; Chemical bonds ; Chlorides ; Chlorine compounds ; Cystic fibrosis ; Development and progression ; Economic impact ; Fibrin ; Fibrinogen ; Fibrinogen - analysis ; Glucocorticoids ; Haptoglobin ; Haptoglobins - analysis ; Immunization ; Infection ; Infections ; Leukocytes (neutrophilic) ; Lung diseases ; Lungs ; Mannheimia haemolytica ; Mannheimia haemolytica - drug effects ; Mannheimia haemolytica - isolation & purification ; Mannheimia haemolytica - pathogenicity ; Medical treatment ; Medicine and Health Sciences ; Methods ; Molecular weight ; Nose ; Oxidation-Reduction ; Pasteurellaceae Infections - drug therapy ; Pasteurellaceae Infections - veterinary ; Pathogenic microorganisms ; Pathogens ; Peptides ; Physical Sciences ; Physiological aspects ; Pneumonia ; Rectum ; Research and Analysis Methods ; Respiratory diseases ; Respiratory tract ; Sodium chloride ; Sodium Chloride - pharmacology ; Vaccines ; Viral infections ; Viruses ; Water ; Water treatment</subject><ispartof>PloS one, 2019-11, Vol.14 (11), p.e0225533-e0225533</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Vulikh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Vulikh et al 2019 Vulikh et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-becea3abd289df4b3a487ec636e2a542d7375312642376bef40a86e67e9d45f53</citedby><cites>FETCH-LOGICAL-c692t-becea3abd289df4b3a487ec636e2a542d7375312642376bef40a86e67e9d45f53</cites><orcidid>0000-0002-1991-3219</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879128/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879128/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2106,2932,23875,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31770402$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Buczinski, Sebastien</contributor><creatorcontrib>Vulikh, Ksenia</creatorcontrib><creatorcontrib>Bassel, Laura L</creatorcontrib><creatorcontrib>Sergejewich, Lauren</creatorcontrib><creatorcontrib>Kaufman, Emily I</creatorcontrib><creatorcontrib>Hewson, Joanne</creatorcontrib><creatorcontrib>MacInnes, Janet I</creatorcontrib><creatorcontrib>Tabatabaei, Saeid</creatorcontrib><creatorcontrib>Caswell, Jeff L</creatorcontrib><title>Effect of tracheal antimicrobial peptide on the development of Mannheimia haemolytica pneumonia in cattle</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Bacterial pneumonia causes significant economic loss to the beef industry and occurs at times of stress and viral infection. Administering antibiotics to at-risk calves is often used to prevent the disease, but alternatives to mass treatment with antibiotics are needed. Tracheal antimicrobial peptide (TAP), a β-defensin naturally produced by bovine airways, has bactericidal activity against the pathogens that cause pneumonia in cattle. However, TAP expression is suppressed by glucocorticoid (stress) and viral infection. We hypothesized that delivering TAP to the respiratory tract would prevent development of pneumonia in calves infected with Mannheimia haemolytica. Clean-catch calves (i.e. obtained prior to contact with the dam) were challenged by aerosol with M. haemolytica, and TAP or water was delivered to the respiratory tract at 0.3, 2 and 6 hours post-infection. TAP treatment did not protect against development of disease. Calves treated with TAP had similar bacterial loads in the nasal cavity and lung compared to calves treated with water. Similarly, TAP treatment did not affect the development of clinical signs, elevated rectal temperatures, or increased levels of blood neutrophils, haptoglobin and fibrinogen that occurred after bacterial challenge. Postmortem gross and histologic lung lesions were also similar in the two groups. To determine why there was a lack of protective effect, we tested the effect of substances in respiratory lining fluid on the bactericidal activity of TAP. Physiologic concentrations of sodium chloride inhibited TAP bactericidal activity in vitro, as did serum at concentrations of 0.62 to 2.5%, but concentrated bronchoalveolar lavage fluid had no consistent effect. These findings suggest that TAP does not have in vivo bactericidal activity against M. haemolytica because of interference by physiological sodium chloride levels and by serum. Thus, administration of TAP may not be effective for prevention of M. haemolytica pneumonia.</description><subject>Alveoli</subject><subject>Animals</subject><subject>Anti-Infective Agents - chemistry</subject><subject>Anti-Infective Agents - pharmacology</subject><subject>Anti-Infective Agents - therapeutic use</subject><subject>Antibiotics</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial agents</subject><subject>Antimicrobial Cationic Peptides - chemistry</subject><subject>Antimicrobial Cationic Peptides - pharmacology</subject><subject>Antimicrobial Cationic Peptides - therapeutic use</subject><subject>Antimicrobial peptides</subject><subject>Autopsy</subject><subject>Bacteria</subject><subject>Bacterial infections</subject><subject>Bactericidal activity</subject><subject>Beef</subject><subject>Beef cattle</subject><subject>Beef industry</subject><subject>Biology and Life Sciences</subject><subject>Bronchoalveolar lavage</subject><subject>Bronchoalveolar Lavage Fluid - microbiology</subject><subject>Bronchus</subject><subject>Calves</subject><subject>Cattle</subject><subject>Cattle Diseases - drug therapy</subject><subject>Chemical bonds</subject><subject>Chlorides</subject><subject>Chlorine compounds</subject><subject>Cystic fibrosis</subject><subject>Development and progression</subject><subject>Economic impact</subject><subject>Fibrin</subject><subject>Fibrinogen</subject><subject>Fibrinogen - analysis</subject><subject>Glucocorticoids</subject><subject>Haptoglobin</subject><subject>Haptoglobins - analysis</subject><subject>Immunization</subject><subject>Infection</subject><subject>Infections</subject><subject>Leukocytes (neutrophilic)</subject><subject>Lung diseases</subject><subject>Lungs</subject><subject>Mannheimia haemolytica</subject><subject>Mannheimia haemolytica - drug effects</subject><subject>Mannheimia haemolytica - isolation & purification</subject><subject>Mannheimia haemolytica - pathogenicity</subject><subject>Medical treatment</subject><subject>Medicine and Health Sciences</subject><subject>Methods</subject><subject>Molecular weight</subject><subject>Nose</subject><subject>Oxidation-Reduction</subject><subject>Pasteurellaceae Infections - 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Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vulikh, Ksenia</au><au>Bassel, Laura L</au><au>Sergejewich, Lauren</au><au>Kaufman, Emily I</au><au>Hewson, Joanne</au><au>MacInnes, Janet I</au><au>Tabatabaei, Saeid</au><au>Caswell, Jeff L</au><au>Buczinski, Sebastien</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of tracheal antimicrobial peptide on the development of Mannheimia haemolytica pneumonia in cattle</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2019-11-26</date><risdate>2019</risdate><volume>14</volume><issue>11</issue><spage>e0225533</spage><epage>e0225533</epage><pages>e0225533-e0225533</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Bacterial pneumonia causes significant economic loss to the beef industry and occurs at times of stress and viral infection. Administering antibiotics to at-risk calves is often used to prevent the disease, but alternatives to mass treatment with antibiotics are needed. Tracheal antimicrobial peptide (TAP), a β-defensin naturally produced by bovine airways, has bactericidal activity against the pathogens that cause pneumonia in cattle. However, TAP expression is suppressed by glucocorticoid (stress) and viral infection. We hypothesized that delivering TAP to the respiratory tract would prevent development of pneumonia in calves infected with Mannheimia haemolytica. Clean-catch calves (i.e. obtained prior to contact with the dam) were challenged by aerosol with M. haemolytica, and TAP or water was delivered to the respiratory tract at 0.3, 2 and 6 hours post-infection. TAP treatment did not protect against development of disease. Calves treated with TAP had similar bacterial loads in the nasal cavity and lung compared to calves treated with water. Similarly, TAP treatment did not affect the development of clinical signs, elevated rectal temperatures, or increased levels of blood neutrophils, haptoglobin and fibrinogen that occurred after bacterial challenge. Postmortem gross and histologic lung lesions were also similar in the two groups. To determine why there was a lack of protective effect, we tested the effect of substances in respiratory lining fluid on the bactericidal activity of TAP. Physiologic concentrations of sodium chloride inhibited TAP bactericidal activity in vitro, as did serum at concentrations of 0.62 to 2.5%, but concentrated bronchoalveolar lavage fluid had no consistent effect. These findings suggest that TAP does not have in vivo bactericidal activity against M. haemolytica because of interference by physiological sodium chloride levels and by serum. Thus, administration of TAP may not be effective for prevention of M. haemolytica pneumonia.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31770402</pmid><doi>10.1371/journal.pone.0225533</doi><tpages>e0225533</tpages><orcidid>https://orcid.org/0000-0002-1991-3219</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2019-11, Vol.14 (11), p.e0225533-e0225533 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2318707384 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Alveoli Animals Anti-Infective Agents - chemistry Anti-Infective Agents - pharmacology Anti-Infective Agents - therapeutic use Antibiotics Antiinfectives and antibacterials Antimicrobial agents Antimicrobial Cationic Peptides - chemistry Antimicrobial Cationic Peptides - pharmacology Antimicrobial Cationic Peptides - therapeutic use Antimicrobial peptides Autopsy Bacteria Bacterial infections Bactericidal activity Beef Beef cattle Beef industry Biology and Life Sciences Bronchoalveolar lavage Bronchoalveolar Lavage Fluid - microbiology Bronchus Calves Cattle Cattle Diseases - drug therapy Chemical bonds Chlorides Chlorine compounds Cystic fibrosis Development and progression Economic impact Fibrin Fibrinogen Fibrinogen - analysis Glucocorticoids Haptoglobin Haptoglobins - analysis Immunization Infection Infections Leukocytes (neutrophilic) Lung diseases Lungs Mannheimia haemolytica Mannheimia haemolytica - drug effects Mannheimia haemolytica - isolation & purification Mannheimia haemolytica - pathogenicity Medical treatment Medicine and Health Sciences Methods Molecular weight Nose Oxidation-Reduction Pasteurellaceae Infections - drug therapy Pasteurellaceae Infections - veterinary Pathogenic microorganisms Pathogens Peptides Physical Sciences Physiological aspects Pneumonia Rectum Research and Analysis Methods Respiratory diseases Respiratory tract Sodium chloride Sodium Chloride - pharmacology Vaccines Viral infections Viruses Water Water treatment |
| title | Effect of tracheal antimicrobial peptide on the development of Mannheimia haemolytica pneumonia in cattle |
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