16S rDNA droplet digital PCR for monitoring bacterial DNAemia in bloodstream infections

Repeated quantitative measurement of bacterial DNA on whole blood has been shown to be a promising method for monitoring bloodstream infection (BSI) with selected bacterial species. To enable broad use of this method, we developed a quantitative droplet digital PCR (ddPCR) method for 16S rDNA. It wa...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	PloS one 2019-11, Vol.14 (11), p.e0224656-e0224656
Hauptverfasser:	Ziegler, Ingrid, Lindström, Sofia, Källgren, Magdalena, Strålin, Kristoffer, Mölling, Paula
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibiotics Bacteria Bacterial genetics Bacterial infections Biology and Life Sciences Blood Deoxyribonucleic acid DNA DNA, Bacterial - blood DNA, Ribosomal - analysis Droplets E coli EDTA Escherichia coli Escherichia coli - isolation & purification Genes Genomes Health aspects HIV Hospitals Human immunodeficiency virus Humans Infection Infections Infectious diseases Instrument industry (Equipment) Laboratories Medicin och hälsovetenskap Medicine Medicine and Health Sciences Methods Monitoring Monitoring methods Pathogenic microorganisms Pathogens Patients Pneumonia Polymerase chain reaction Process controls Research and Analysis Methods rRNA 16S Sepsis Sepsis - diagnosis Sepsis - microbiology Species Staphylococcal Infections - diagnosis Staphylococcal Infections - microbiology Staphylococcus aureus Staphylococcus aureus - isolation & purification Staphylococcus aureus infections Staphylococcus infections Streptococcus infections Streptococcus pneumoniae
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!