Sealing agent reduces formation of single and dual-species biofilms of Candida albicans and Enterococcus faecalis on screw joints at the abutment/implant interface
The aim of this research was to evaluate the efficacy of a commercial sealing agent at the abutment/implant interface against microleakage of single and dual-species biofilms of Candida albicans and Enterococcus faecalis into external hexagon (EH) and Morse taper (MT) prosthetic connections. A total...
Gespeichert in:
| Veröffentlicht in: | PloS one 2019-10, Vol.14 (10), p.e0223148-e0223148 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0223148 |
|---|---|
| container_issue | 10 |
| container_start_page | e0223148 |
| container_title | PloS one |
| container_volume | 14 |
| creator | Sousa, Cecília Alves de Conforte, Jadison Junio Caiaffa, Karina Sampaio Duque, Cristiane Assunção, Wirley Gonçalves |
| description | The aim of this research was to evaluate the efficacy of a commercial sealing agent at the abutment/implant interface against microleakage of single and dual-species biofilms of Candida albicans and Enterococcus faecalis into external hexagon (EH) and Morse taper (MT) prosthetic connections. A total of 216 samples of implants and their abutments were tested. Six groups (n = 36) were evaluated based on biofilm and period of incubation (7 and 14 days). The implant connections EH and MT (n = 18) were divided according to the use of the material (n = 9) (EH-T and MT-T: with the sealing agent; EH-C and MT-C: control). The biofilms were analyzed by microbial counting (CFU/mL) and SEM analysis and photographs of the material in the screw joints were also taken. Data were analyzed by Student t test, two-way ANOVA and Bonferroni test. For the single-species biofilms, there was a significant reduction in the growth of E. faecalis when compared MT-C and MT-T or EH-C and EH-T at 7 and 14 days. The same was observed for C. albicans biofilms. For dual-species biofilms of E. faecalis and C. albicans, the sealing agent was more effective in preventing microbial infiltration into the MT connection at 14 days, while microbial infiltration did not occur into EH connections even in absence of the sealing agent for both periods of evaluation. Overall, these data suggest that the presence of the sealing agent reduces or eliminates the microleakage of E. faecalis and C. albicans biofilms into the implants regardless of the period of incubation. |
| doi_str_mv | 10.1371/journal.pone.0223148 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2307721824</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A603472013</galeid><doaj_id>oai_doaj_org_article_6af4fae4f235445eaa22bcb8fb61a02c</doaj_id><sourcerecordid>A603472013</sourcerecordid><originalsourceid>FETCH-LOGICAL-c758t-f904c37915832dc304ae8671f4dd6579305b837c21f2a61b28342b3281f6abf73</originalsourceid><addsrcrecordid>eNqNk1trFDEUxwdRbK1-A9EBQfRht7lNJvMilKVqoVCw6ms4k0l2s2Qm2yTj5fP4Rc1st2VX-iDzkCHnd_7nknOK4iVGc0xrfLr2YxjAzTd-0HNECMVMPCqOcUPJjBNEH-_9HxXPYlwjVFHB-dPiiGJOG0ya4-LPtQZnh2UJSz2kMuhuVDqWxocekvVD6U0Zs93pEoau7EZws7jRymaotd5Y18eJWWSr7aAE11oFQ9zS50PSwSuv1JglQascKtNDGVXQP8u1t0PKZCrTKsu3Y-pzDqe23zjIudjJ24DSz4snBlzUL3bnSfHt4_nXxefZ5dWni8XZ5UzVlUgz0yCmaN3gSlDSKYoYaMFrbFjX8apuKKpaQWtFsCHAcUsEZaSlRGDDoTU1PSle3-punI9y198oCUV1TbAgLBMXt0TnYS03wfYQfksPVm4vfFhKCMkqpyUHw3LJzBBaMVZpAEJa1QrTcgyIqKz1YRdtbHvdqVx6AHcgemgZ7Eou_Q_JBWINn9J9txMI_mbUMcneRqVdbp724zZvgQUmaELf_IM-XN2OWkIuwA7G57hqEpVnHFFWE4RppuYPUPnrdG9VHsY8E_rQ4f2BQ2aS_pWWMMYoL66__D979f2QfbvHrvIcp1X0bpzGNh6C7BZUwccYtLlvMkZy2qW7bshpl-Rul7Lbq_0Hune6Wx76F-sBG3U</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2307721824</pqid></control><display><type>article</type><title>Sealing agent reduces formation of single and dual-species biofilms of Candida albicans and Enterococcus faecalis on screw joints at the abutment/implant interface</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Sousa, Cecília Alves de ; Conforte, Jadison Junio ; Caiaffa, Karina Sampaio ; Duque, Cristiane ; Assunção, Wirley Gonçalves</creator><contributor>Sturtevant, Joy</contributor><creatorcontrib>Sousa, Cecília Alves de ; Conforte, Jadison Junio ; Caiaffa, Karina Sampaio ; Duque, Cristiane ; Assunção, Wirley Gonçalves ; Sturtevant, Joy</creatorcontrib><description>The aim of this research was to evaluate the efficacy of a commercial sealing agent at the abutment/implant interface against microleakage of single and dual-species biofilms of Candida albicans and Enterococcus faecalis into external hexagon (EH) and Morse taper (MT) prosthetic connections. A total of 216 samples of implants and their abutments were tested. Six groups (n = 36) were evaluated based on biofilm and period of incubation (7 and 14 days). The implant connections EH and MT (n = 18) were divided according to the use of the material (n = 9) (EH-T and MT-T: with the sealing agent; EH-C and MT-C: control). The biofilms were analyzed by microbial counting (CFU/mL) and SEM analysis and photographs of the material in the screw joints were also taken. Data were analyzed by Student t test, two-way ANOVA and Bonferroni test. For the single-species biofilms, there was a significant reduction in the growth of E. faecalis when compared MT-C and MT-T or EH-C and EH-T at 7 and 14 days. The same was observed for C. albicans biofilms. For dual-species biofilms of E. faecalis and C. albicans, the sealing agent was more effective in preventing microbial infiltration into the MT connection at 14 days, while microbial infiltration did not occur into EH connections even in absence of the sealing agent for both periods of evaluation. Overall, these data suggest that the presence of the sealing agent reduces or eliminates the microleakage of E. faecalis and C. albicans biofilms into the implants regardless of the period of incubation.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0223148</identifier><identifier>PMID: 31639129</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Biofilms ; Biofilms - growth & development ; Biology and Life Sciences ; Bone Screws - adverse effects ; Bone Screws - microbiology ; Candida albicans ; Candida albicans - drug effects ; Candida albicans - growth & development ; Candida albicans - pathogenicity ; Composite materials ; Control ; Dental Abutments - microbiology ; Dental Implants - microbiology ; Dental Materials - pharmacology ; Dental Stress Analysis ; Dentistry ; Disease ; Engineering and Technology ; Enterococcus ; Enterococcus faecalis ; Enterococcus faecalis - drug effects ; Enterococcus faecalis - growth & development ; Growth ; Humans ; Infiltration ; Materials Testing - methods ; Medicine and Health Sciences ; Microbial mats ; Microbiota ; Microorganisms ; Pediatrics ; Prostheses ; Prostheses and implants ; Public health ; Research and Analysis Methods ; Sealants ; Sealing ; Species ; Studies ; Surgical implants ; Testing ; Titanium - chemistry ; Titanium - therapeutic use ; Transplants & implants ; Variance analysis</subject><ispartof>PloS one, 2019-10, Vol.14 (10), p.e0223148-e0223148</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Alves de Sousa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Alves de Sousa et al 2019 Alves de Sousa et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-f904c37915832dc304ae8671f4dd6579305b837c21f2a61b28342b3281f6abf73</citedby><cites>FETCH-LOGICAL-c758t-f904c37915832dc304ae8671f4dd6579305b837c21f2a61b28342b3281f6abf73</cites><orcidid>0000-0002-4192-5486</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804967/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804967/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31639129$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Sturtevant, Joy</contributor><creatorcontrib>Sousa, Cecília Alves de</creatorcontrib><creatorcontrib>Conforte, Jadison Junio</creatorcontrib><creatorcontrib>Caiaffa, Karina Sampaio</creatorcontrib><creatorcontrib>Duque, Cristiane</creatorcontrib><creatorcontrib>Assunção, Wirley Gonçalves</creatorcontrib><title>Sealing agent reduces formation of single and dual-species biofilms of Candida albicans and Enterococcus faecalis on screw joints at the abutment/implant interface</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The aim of this research was to evaluate the efficacy of a commercial sealing agent at the abutment/implant interface against microleakage of single and dual-species biofilms of Candida albicans and Enterococcus faecalis into external hexagon (EH) and Morse taper (MT) prosthetic connections. A total of 216 samples of implants and their abutments were tested. Six groups (n = 36) were evaluated based on biofilm and period of incubation (7 and 14 days). The implant connections EH and MT (n = 18) were divided according to the use of the material (n = 9) (EH-T and MT-T: with the sealing agent; EH-C and MT-C: control). The biofilms were analyzed by microbial counting (CFU/mL) and SEM analysis and photographs of the material in the screw joints were also taken. Data were analyzed by Student t test, two-way ANOVA and Bonferroni test. For the single-species biofilms, there was a significant reduction in the growth of E. faecalis when compared MT-C and MT-T or EH-C and EH-T at 7 and 14 days. The same was observed for C. albicans biofilms. For dual-species biofilms of E. faecalis and C. albicans, the sealing agent was more effective in preventing microbial infiltration into the MT connection at 14 days, while microbial infiltration did not occur into EH connections even in absence of the sealing agent for both periods of evaluation. Overall, these data suggest that the presence of the sealing agent reduces or eliminates the microleakage of E. faecalis and C. albicans biofilms into the implants regardless of the period of incubation.</description><subject>Biofilms</subject><subject>Biofilms - growth & development</subject><subject>Biology and Life Sciences</subject><subject>Bone Screws - adverse effects</subject><subject>Bone Screws - microbiology</subject><subject>Candida albicans</subject><subject>Candida albicans - drug effects</subject><subject>Candida albicans - growth & development</subject><subject>Candida albicans - pathogenicity</subject><subject>Composite materials</subject><subject>Control</subject><subject>Dental Abutments - microbiology</subject><subject>Dental Implants - microbiology</subject><subject>Dental Materials - pharmacology</subject><subject>Dental Stress Analysis</subject><subject>Dentistry</subject><subject>Disease</subject><subject>Engineering and Technology</subject><subject>Enterococcus</subject><subject>Enterococcus faecalis</subject><subject>Enterococcus faecalis - drug effects</subject><subject>Enterococcus faecalis - growth & development</subject><subject>Growth</subject><subject>Humans</subject><subject>Infiltration</subject><subject>Materials Testing - methods</subject><subject>Medicine and Health Sciences</subject><subject>Microbial mats</subject><subject>Microbiota</subject><subject>Microorganisms</subject><subject>Pediatrics</subject><subject>Prostheses</subject><subject>Prostheses and implants</subject><subject>Public health</subject><subject>Research and Analysis Methods</subject><subject>Sealants</subject><subject>Sealing</subject><subject>Species</subject><subject>Studies</subject><subject>Surgical implants</subject><subject>Testing</subject><subject>Titanium - chemistry</subject><subject>Titanium - therapeutic use</subject><subject>Transplants & implants</subject><subject>Variance analysis</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1trFDEUxwdRbK1-A9EBQfRht7lNJvMilKVqoVCw6ms4k0l2s2Qm2yTj5fP4Rc1st2VX-iDzkCHnd_7nknOK4iVGc0xrfLr2YxjAzTd-0HNECMVMPCqOcUPJjBNEH-_9HxXPYlwjVFHB-dPiiGJOG0ya4-LPtQZnh2UJSz2kMuhuVDqWxocekvVD6U0Zs93pEoau7EZws7jRymaotd5Y18eJWWSr7aAE11oFQ9zS50PSwSuv1JglQascKtNDGVXQP8u1t0PKZCrTKsu3Y-pzDqe23zjIudjJ24DSz4snBlzUL3bnSfHt4_nXxefZ5dWni8XZ5UzVlUgz0yCmaN3gSlDSKYoYaMFrbFjX8apuKKpaQWtFsCHAcUsEZaSlRGDDoTU1PSle3-punI9y198oCUV1TbAgLBMXt0TnYS03wfYQfksPVm4vfFhKCMkqpyUHw3LJzBBaMVZpAEJa1QrTcgyIqKz1YRdtbHvdqVx6AHcgemgZ7Eou_Q_JBWINn9J9txMI_mbUMcneRqVdbp724zZvgQUmaELf_IM-XN2OWkIuwA7G57hqEpVnHFFWE4RppuYPUPnrdG9VHsY8E_rQ4f2BQ2aS_pWWMMYoL66__D979f2QfbvHrvIcp1X0bpzGNh6C7BZUwccYtLlvMkZy2qW7bshpl-Rul7Lbq_0Hune6Wx76F-sBG3U</recordid><startdate>20191022</startdate><enddate>20191022</enddate><creator>Sousa, Cecília Alves de</creator><creator>Conforte, Jadison Junio</creator><creator>Caiaffa, Karina Sampaio</creator><creator>Duque, Cristiane</creator><creator>Assunção, Wirley Gonçalves</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-4192-5486</orcidid></search><sort><creationdate>20191022</creationdate><title>Sealing agent reduces formation of single and dual-species biofilms of Candida albicans and Enterococcus faecalis on screw joints at the abutment/implant interface</title><author>Sousa, Cecília Alves de ; Conforte, Jadison Junio ; Caiaffa, Karina Sampaio ; Duque, Cristiane ; Assunção, Wirley Gonçalves</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-f904c37915832dc304ae8671f4dd6579305b837c21f2a61b28342b3281f6abf73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Biofilms</topic><topic>Biofilms - growth & development</topic><topic>Biology and Life Sciences</topic><topic>Bone Screws - adverse effects</topic><topic>Bone Screws - microbiology</topic><topic>Candida albicans</topic><topic>Candida albicans - drug effects</topic><topic>Candida albicans - growth & development</topic><topic>Candida albicans - pathogenicity</topic><topic>Composite materials</topic><topic>Control</topic><topic>Dental Abutments - microbiology</topic><topic>Dental Implants - microbiology</topic><topic>Dental Materials - pharmacology</topic><topic>Dental Stress Analysis</topic><topic>Dentistry</topic><topic>Disease</topic><topic>Engineering and Technology</topic><topic>Enterococcus</topic><topic>Enterococcus faecalis</topic><topic>Enterococcus faecalis - drug effects</topic><topic>Enterococcus faecalis - growth & development</topic><topic>Growth</topic><topic>Humans</topic><topic>Infiltration</topic><topic>Materials Testing - methods</topic><topic>Medicine and Health Sciences</topic><topic>Microbial mats</topic><topic>Microbiota</topic><topic>Microorganisms</topic><topic>Pediatrics</topic><topic>Prostheses</topic><topic>Prostheses and implants</topic><topic>Public health</topic><topic>Research and Analysis Methods</topic><topic>Sealants</topic><topic>Sealing</topic><topic>Species</topic><topic>Studies</topic><topic>Surgical implants</topic><topic>Testing</topic><topic>Titanium - chemistry</topic><topic>Titanium - therapeutic use</topic><topic>Transplants & implants</topic><topic>Variance analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sousa, Cecília Alves de</creatorcontrib><creatorcontrib>Conforte, Jadison Junio</creatorcontrib><creatorcontrib>Caiaffa, Karina Sampaio</creatorcontrib><creatorcontrib>Duque, Cristiane</creatorcontrib><creatorcontrib>Assunção, Wirley Gonçalves</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sousa, Cecília Alves de</au><au>Conforte, Jadison Junio</au><au>Caiaffa, Karina Sampaio</au><au>Duque, Cristiane</au><au>Assunção, Wirley Gonçalves</au><au>Sturtevant, Joy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sealing agent reduces formation of single and dual-species biofilms of Candida albicans and Enterococcus faecalis on screw joints at the abutment/implant interface</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2019-10-22</date><risdate>2019</risdate><volume>14</volume><issue>10</issue><spage>e0223148</spage><epage>e0223148</epage><pages>e0223148-e0223148</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The aim of this research was to evaluate the efficacy of a commercial sealing agent at the abutment/implant interface against microleakage of single and dual-species biofilms of Candida albicans and Enterococcus faecalis into external hexagon (EH) and Morse taper (MT) prosthetic connections. A total of 216 samples of implants and their abutments were tested. Six groups (n = 36) were evaluated based on biofilm and period of incubation (7 and 14 days). The implant connections EH and MT (n = 18) were divided according to the use of the material (n = 9) (EH-T and MT-T: with the sealing agent; EH-C and MT-C: control). The biofilms were analyzed by microbial counting (CFU/mL) and SEM analysis and photographs of the material in the screw joints were also taken. Data were analyzed by Student t test, two-way ANOVA and Bonferroni test. For the single-species biofilms, there was a significant reduction in the growth of E. faecalis when compared MT-C and MT-T or EH-C and EH-T at 7 and 14 days. The same was observed for C. albicans biofilms. For dual-species biofilms of E. faecalis and C. albicans, the sealing agent was more effective in preventing microbial infiltration into the MT connection at 14 days, while microbial infiltration did not occur into EH connections even in absence of the sealing agent for both periods of evaluation. Overall, these data suggest that the presence of the sealing agent reduces or eliminates the microleakage of E. faecalis and C. albicans biofilms into the implants regardless of the period of incubation.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31639129</pmid><doi>10.1371/journal.pone.0223148</doi><tpages>e0223148</tpages><orcidid>https://orcid.org/0000-0002-4192-5486</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2019-10, Vol.14 (10), p.e0223148-e0223148 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2307721824 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Biofilms Biofilms - growth & development Biology and Life Sciences Bone Screws - adverse effects Bone Screws - microbiology Candida albicans Candida albicans - drug effects Candida albicans - growth & development Candida albicans - pathogenicity Composite materials Control Dental Abutments - microbiology Dental Implants - microbiology Dental Materials - pharmacology Dental Stress Analysis Dentistry Disease Engineering and Technology Enterococcus Enterococcus faecalis Enterococcus faecalis - drug effects Enterococcus faecalis - growth & development Growth Humans Infiltration Materials Testing - methods Medicine and Health Sciences Microbial mats Microbiota Microorganisms Pediatrics Prostheses Prostheses and implants Public health Research and Analysis Methods Sealants Sealing Species Studies Surgical implants Testing Titanium - chemistry Titanium - therapeutic use Transplants & implants Variance analysis |
| title | Sealing agent reduces formation of single and dual-species biofilms of Candida albicans and Enterococcus faecalis on screw joints at the abutment/implant interface |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T03%3A31%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sealing%20agent%20reduces%20formation%20of%20single%20and%20dual-species%20biofilms%20of%20Candida%20albicans%20and%20Enterococcus%20faecalis%20on%20screw%20joints%20at%20the%20abutment/implant%20interface&rft.jtitle=PloS%20one&rft.au=Sousa,%20Cec%C3%ADlia%20Alves%20de&rft.date=2019-10-22&rft.volume=14&rft.issue=10&rft.spage=e0223148&rft.epage=e0223148&rft.pages=e0223148-e0223148&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0223148&rft_dat=%3Cgale_plos_%3EA603472013%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2307721824&rft_id=info:pmid/31639129&rft_galeid=A603472013&rft_doaj_id=oai_doaj_org_article_6af4fae4f235445eaa22bcb8fb61a02c&rfr_iscdi=true |